• The Korean Journal of Ecology
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• 제19권3호
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• pp.201-207
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• 1996

Selection of Al tolerant woody plants, and possibility of reforestation with the Al tolerant plants in soil conditions with different Al content, topography and slope exposures were studied on the slopes around Yeocheon Industrial Complex. Root growth in length of plants grown in 1/2 Steinberg solution decreased with increased Al concentrations of the solution. Relative root length showed that Paulownia coreana, Celtis sinensis and Firmiana simplex were sensitive to $500\muM$ Al, Pinus koraiensis, Alnus japonica and Ligustrum japonicum were intermediate, and Pinus rigida, P. densiflora and P. thunbergii were tolerant to $1, 000\muM$ Al. Coniferous plants appeared to be more tolerant to Al than deciduous ones. Soil pH was 4.2 and Al content was 509 ppm in average around the Yeocheon industrial complex. Al content and soil acidity were more deteriorated on the foothill and slope facing to the pollution source from the industrial complex than on the ridge and opposite slope.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.805-814
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• 2008

Liming of acidic soils can prevent aluminum toxicity and improve crop production. Some maize lines show aluminum (Al) tolerance, and exudation of organic acids by roots has been considered to represent an important mechanism involved in the tolerance. However, there is no information about the impact of liming on the structures of bacterial and fungal communities in Cerrado soil, nor if there are differences between the microbial communities from the rhizospheres of Al-tolerant and Al-sensitive maize lines. This study evaluated the effects of liming on the structure of bacterial and fungal communities in bulk soil and rhizospheres of Al-sensitive and Al-tolerant maize (Zea mays L.) lines cultivated in Cerrado soil by PCR-DGGE, 30 and 90 days after sowing. Bacterial fingerprints revealed that the bacterial communities from rhizospheres were more affected by aluminum stress in soil than by the maize line (Al-sensitive or Al-tolerant). Differences in bacterial communities were also observed over time (30 and 90 days after sowing), and these occurred mainly in the Actinobacteria. Conversely, fungal communities from the rhizosphere were weakly affected either by liming or by the rhizosphere, as observed from the DGGE profiles. Furthermore, only a few differences were observed in the DGGE profiles of the fungal populations during plant development when compared with bacterial communities. Cloning and sequencing of 16S rRNA gene fragments obtained from dominant DGGE bands detected in the bacterial profiles of the Cerrado bulk soil revealed that Actinomycetales and Rhizobiales were among the dominant ribotypes.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1169-1179
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• 2020

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
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• pp.12-12
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• 2010

We developed droplet-vitrification protocol, a combination of droplet-freezing and solution-based vitrification, and applied to germplasm collections of garlic, potato, lily as well as cell lines, including hairy roots, somatic embryos. To establish a garlic cryobank, four Korean garlic field collections at Danyang, Suwon, Mokpo and Namhae were cryopreserved last five years. The protocol applied consisted of preculture for 3-4 days at $10^{\circ}C$ on solid MS medium with 0.3M sucrose, loading for 40 min in liquid medium with 35% PVS3, dehydration with PVS3 for 150 min, cooling in $5{\mu}l$ droplets of PVS3 placed on aluminum foil strips by dipping these strips in liquid nitrogen, warming them by plunging the foil strips into pre-heated($40^{\circ}C$) 0.8M sucrose solution for 30s. A total of over 900 accessions of garlic were stored in liquid nitrogen for long-term conservation using unripe inflorescences, cloves or bulbils. Twelve alternative plant vitrification solutions were designed by modifying cryoprotectant concentrations from the original PVS2 and PVS3. The results suggest that PVS2-based vitrification solutions with increased glycerol and sucrose and/or decreased DMSO and EG concentrations can be applied for medium size explants which are tolerant to chemical toxicity and moderately sensitive to osmotic stress. PVS3 and variants can be used widely when samples are heterogeneous, of large size and/or very sensitive to chemical toxicity and tolerant to osmotic stress.

• The Korean Journal of Ecology
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• 제26권3호
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• pp.143-150
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• 2003

한국은 양적으로 강수량이 풍부하지만 강우현상이 편중되어 매년 일정기간의 가뭄기를 겪는다. 더구나 그 토양은 대부분 화강암에서 유래한 거친 입자의 것으로서 보수력이 낮다. 수 종의 참나무류와 소나무의 수분스트레스에 대한 반응 결과는 수분수지가 식생의 분포를 결정하는 주 요인임을 보여주었다. 뿐만 아니라 몇몇 상록식물의 월동기간 중 내한성 기작에 기인한 탈수 정도는 자연상태에서 그들의 분포와 밀접하게 관계되었다. 수분스트레스 처리 실험 결과 소나무는 건조에 대해 강한 내성을 보였지만 그 유식물은 가뭄기에 높은 고사율을 나타내었다. 그 고사율은 지소의 토양수분함량에 비례하는 경향이었다. 가뭄이 심한 6월 중 삼림토양의 수분함량과 수분스트레스 처리 실험에서 소나무 유식물이 영구위조점에 도달한 시점에서 배지토양의 수분함량을 비교한 결과는 가뭄기에 소나무 유식물의 높은 고사율이 수분부족에 기인함을 입증하였다. 대기오염물질 폭로실험 중 측정된 시료식물의 수분포텐셜은 시료식물에서 가시피해의 발생과 그 내성에 수분요인이 관계될 가능성을 보여주었다. 야외 현장 조사와 실내실험 결과 둘 다에서 심한 대기오염에 노출된 식물은 그렇지 않은 것과 비교하여 수분소실이 빠르게 일어나는데, 그것은 오염물질에 의한 잎 표면의 손상 정도와 관계되었다. 산성 토양에서 높은 함유량을 보이는 알루미늄은 식물의 뿌리생장을 억 제하거나 비정상적 분포를 가져와 수분스트레스를 유발하였다. 대기오염과 토양 산성화에 기인한 이러한 수분스트레스 효과는 한국의 기후와 토양이 가지는 기존의 수분결핍효과에 덧붙여져 삼림쇠퇴를 유발하는 주 요인으로 작용할 가능성이 있다. 토양의 유기물 함량을 높여 포장용수량 증가에 기여할 수 있는 sludge는 산성토양을 개량하는데 기존의 개량제 못지않은 효과를 보였고, 낙엽 추출물 또한 알루미늄에 의한 수분스트레스 효과를 완화시키는데 기여하였다. 이러한 결과를 종합하여 한국에서 삼림쇠퇴를 유발할 수 있는 스트레스요인들의 잠재적 상호작용을 모식화하였다. 나아가 그것을 완화할 수 있는 복원방안을 토양개량과 식생복원의 측면에서 제시하였다.