• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.125-131
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• 2015

Currently, taxol is mainly extracted from the bark of yews; however, this method can not meet its increasing demand on the market because yews grow very slowly and are a rare and endangered species belonging to first-level conservation plants. Recently, increasing efforts have been made to develop alternative means of taxol production; microbe fermentation would be a very promising method to increase the production scale of taxol. To determine the activities of the taxol extracted from endophytic fungus N. sylviforme HDFS4-26 in inhibiting the growth and causing the apoptosis of cancer cells, on comparison with the taxol extracted from the bark of yew, we used cellular morphology, cell counting kit (CCK-8) assay, staining (HO33258/PI and Giemsa), DNA agarose gel electrophoresis and flow cytometry (FCM) analyses to determine the apoptosis status of breast cancer MCF-7 cells, cervical cancer HeLa cells and ovarian cancer HO8910 cells. Our results showed that the fungal taxol inhibited the growth of MCF-7, HeLa and HO8910 cells in a dose-and time-dependent manner. IC50 values of fungal taxol for HeLa, MCF-7 and HO8910 cells were $0.1-1.0{\mu}g/ml$, $0.001-0.01{\mu}g/ml$ and $0.01-0.1{\mu}g/ml$, respectively. The fungal taxol induced these tumor cells to undergo apoptosis with typical apoptotic characteristics, including morphological changes for chromatin condensation, chromatin crescent formation, nucleus fragmentation, apoptotic body formation and G2/M cell cycle arrest. The fungal taxol at the $0.01-1.0{\mu}g/ml$ had significant effects of inducing apoptosis between 24-48 h, which was the same as that of taxol extracted from yews. This study offers important information and a new resource for the production of an important anticancer drug by endofungus fermentation.

• 대한한의학회지
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• 제17권2호
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• pp.34-47
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• 1996

전국민의료보험제도가 실시된 이후 의료 서비스에 대한 수요는 외래 진료와 입원진 모두 현저한 상승 현상을 보여주고 있다. 그러나 이에 반해 한방의료에 대한 수요는 한방의료보험제도의 부분적 시행에도 불구하고 상대적으로 저조한 실정이며, 의료시장에서의 점유율도 점차 감소하고 있는 추세에 있다. 따라서 한방의료를 국민 속으로 널리 확산시키고 양방의료에 대한 대체 기능을 제고시키려면 소비자들이 보다 쉽게 한방의료에 접근할 수 있는 장치가 마련되어야 하며, 그 중에서 가장 핵심적인 요소는 한방의료의 가격을 적정한 수준으로 유지하는 것이다. 본고에서는 한방의료기관이 현재의 경영 상태를 유지하면서도 한방의료의 대중화를 촉진시킬 수 있는 한방의료의 적정 가격을 추계하여 제시하였다. 본 연구 결과에 의하면 한방의료의 가격탄력성은 약 5.8로 높게 나타나 가격이 하락할 때 한방의료에 대한 수요가 그 이상으로 증가할 것으로 예상되어 전체적으로 경영수지는 호전될 것으로 추계되었다. 첩약가의 인하가 한방의료기관의 경영수지에 미치는 영향을 분석한 결과에 의하면 한의사의 추가공급에 별다른 제한이 없는 경우, 가격탄력성이 6일 때 첩약가를 20%, 40%, 50%씩 인하하면 수입이 각각 76%, 104%, 100% 증가하는 것으로 추계되었다. 또 가격탄력성이 5인 경우에는 수입이 각각 60%, 80%, 75% 증가하고, 가격탄력성이 4인 경우에는 수입이 각각 44%, 56%, 50% 증가하는 것으로 추계되었다. 그리고 이윤율을 첩약가 인하 이전인 50% 수준으로 유지하고자 할 때 최대로 가능한 첩약가의 인하 폭을 산정한 결과, (i)가격탄력성이 현재와 같이 6인 경우에는 최대 가격인하율이 33.3%이며, 이 때 첩약의 이용량은 200% 증가하고 수입은 2배가 되는 것으로 나타난다. (ii)가격탄력성이 5인 경우에는 가격을 30% 인하할 때 지금과 같은 이윤을 누릴 수 있으며, 이 때 한의원의 수입은 75% 증가하는 것으로 추정된다. (iii)가격탄력성이 4일 때의 최대 가격인하율은 25%로서 이 경우에 수입은 50% 증가하는 것으로 추정된다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1684-1688
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• 2017

Objective: From a review of published information on genetic association studies, a meta-analysis was conducted to determine the influence of the genes ${\kappa}-casein$ (CSN3) and ${\beta}-lactoglobulin$ (LGB) on milk yield traits in Holstein, Jersey, Brown Swiss, and Fleckvieh. Methods: The GLIMMIX procedure was used to analyze milk production and percentage of protein and fat in milk. Models included the main effects and all their possible two-way interactions; not estimable effects and non-significant (p>0.05) two-way interactions were dropped from the models. The three traits analyzed used Poisson distribution and a log link function and were determined with the Interactive Data Analysis of SAS software. Least square means and multiple mean comparisons were obtained and performed for significant main effects and their interactions (p<0.0255). Results: Interaction of breed by gene showed that Holstein and Fleckvieh were the breeds on which CSN3 ($6.01%{\pm}0.19%$ and $5.98%{\pm}0.22%$), and LGB ($6.02%{\pm}0.19%$ and $5.70%{\pm}0.22%$) have the greatest influence. Interaction of breed by genotype nested in the analyzed gene indicated that Holstein and Jersey showed greater influence of the CSN3 AA genotype, $6.04%{\pm}0.22%$ and $5.59%{\pm}0.31%$ than the other genotypes, while LGB AA genotype had the largest influence on the traits analyzed, $6.05%{\pm}0.20%$ and $5.60%{\pm}0.19%$, respectively. Furthermore, interaction of type of statistical model by genotype nested in the analyzed gene indicated that CSN3 and LGB genes had similar behavior, maintaining a difference of more than 7% across analyzed genotypes. These results could indicate that both Holstein and Jersey have had lower substitution allele effect in selection programs that include CSN3 and LGB genes than Brown Swiss and Fleckvieh. Conclusion: Breed determined which genotypes had the greatest association with analyzed traits. The mixed model based in Bayesian or Ridge Regression was the best alternative to analyze CSN3 and LGB gene effects on milk yield and protein and fat percentages.

• 한국산학기술학회논문지
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• 제17권2호
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• pp.246-251
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• 2016

도로조명 중 누출광을 고려하지 않은 보안등이 아직도 많이 사용되어 주거지 침입광이 발생되고 있다. 이러한 도로조명의 침입광 저감을 위해 차광판이 설치되고 있지만, 효과가 미미하거나 미적 요소 및 안전성에 문제가 있다. 본 연구에서는 침입광 효율적인 저감을 위해 차광판 구조별 저감 특성을 연구하였으며, 그 방법으로 조명시뮬레이션 프로그램인 Relux 활용하였다. 또한 시뮬레이션의 값의 정확성을 알기 위해 실제 측정값과 검증하였다. 그 결과 (상)도색 글로브를 제외한 모든 차광판에서 후사광 저감 효과가 뛰어났으며, 도장형 차광판의 경우 전사광도 줄어들어 글레어 저감도 효율 적인 것으로 확인되었다. (상)도색 글로브는 위쪽으로 가는 빛을 아래로 반사시켜 후사광 전사광 모두 증가하였지만, 스카이 글로우(Sky glow) 저감에 효율적인 차광판으로 확인되었다. 시뮬레이션 값과 실측값은 90 %의 일치률을 보였다.

• 유통과학연구
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• 제10권11호
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• pp.79-88
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• 2012

Purpose - The objective of this paper is to suggest that a company's CRM activities have an effect on customer loyalty in the Korean retail industry. Typically, Korean customers use large local marts with convenience in the absence of any other choice. Therefore, this study aims to shed light on the fact that customers do not break away from their preferred retail stores, either owing to their stringent loyalty (the lie loyalty) or difficulty in turning to alternative choices. Research design, data, methodology - By surveying a sample of 200 hyper-markets through a questionnaire, and excluding dubious and missing responses, I obtained 181 samples to be included in the empirical analysis. The survey was conducted for two weeks during October 2011. AMOS and SPSS18 statistical packages were used for conducting statistical analysis for this study. This paper was developed using the concept of customer equity on CRM, which is known to have a positive impact on customer loyalty through the satisfaction and switching-barrier parameters. The hypothesis of this paper is that customer equity is composed of relationship equity, value equity and brand equity, and that the relationship equity variable has positive effects on the value equity and brand equity amongst other types of customer equity. Moreover, customer equity influences customer loyalty through parameters including customer satisfaction and switching costs in the Korean retail industry. Results - According to the results of the analysis, it was confirmed that relationship value had a positive effect (+) on all variables, including the perceived QoS (Quality of Service), store brand images, economic value, and store convenience. It was also confirmed that the assumption that the perceived QoS (Quality of Service), economic value, and store convenience had a positive effect on customer satisfaction was shown to be statistically significant, with a p-value below 0.05. Only the store brand value variable had an effect on the switching-cost variable with respect to the causal sequence of the variables, including the perceived QoS, store brand value, economic value, and store convenience. The remaining variables did not seem to influence the switching-cost variable. On the other hand, another effect showed that customer satisfaction had a statistically significant influence on the switching-costs variable. Moreover, the customer satisfaction and switching-cost variables also had a statistical influence on customer loyalty. Conclusions - The CRM activities had an influence on various variables (including perceived QoS, perceived economic value, store brand value, and store convenience) pertaining to customer values. Customer satisfaction and switching-cost had some effects on customer loyalty as a parameter. This confirms that stringent loyalty exists with respect to customer loyalty in the retail industry. The fact that the variable had such a statistically significant influence on the switching-cost and store brand equity variables means that consumers react to the reputation of a brand, confidence about the store, and quality confidence. The implications of this study in the retail industry should be further extended to devise strategies for customer retention.

• 한국지리정보학회지
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• 제13권3호
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• pp.14-28
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• 2010

본 연구의 목적은 공간적 분포 특성만을 고려하고 있는 기존의 핫스팟분석에 대한 대안적인 방법으로서 공간상에서 나타나는 사건간의 인과관계를 시간영역으로까지 확장하여 동시적 분석이 가능한 시공간분석 방법을 제안하는 것이다. 분석방법으로는 먼저 지리정보시스템을 이용하여 지방중소도시인 M시의 범죄자료를 데이터화 하였고, Ripley K함수와 시공간검정통계량 분석을 통해 M시의 범죄분포 패턴을 지도화 하였다. 연구결과, 범죄위험도가 유의미하게 높은 지역들이 나타났으며, 이들 시공간적 범죄 집중지역들은 기존의 공간분포만을 고려한 범죄분포 패턴과는 다소 차이가 있음을 발견할 수 있었다. 본 연구결과는 시공간적인 범죄분포 특성에 맞는 맞춤형의 경찰 인력 배치와 배분, 그리고 치안행정 서비스 등의 조정을 위한 참고자료로서, 또한 시공간적인 집중을 보이는 이들 지역을 중심으로 물리적 환경 변화의 유도와 공간이용의 개선 효과를 통해 범죄율을 줄여나가는 범죄예방 활동 및 정책수립을 위한 기초자료로도 유용하게 활용될 수 있을 것으로 기대된다.

• Archives of Plastic Surgery
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• 제37권1호
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• pp.99-103
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• 2010

Purpose: Blow-out fractures are reduced through transcutaneous or transconjunctival incisions. But the field of orbital surgery is difficult due to lack of visualization of fracture site, blind dissection of orbital floor, susceptibility of injury of orbital structures. In these situations, the former technique of using an antral balloon catheter has advantages over other methods for reconstruction because of its rapidity, simplicity, and inexpensiveness. Furthermore, the antral balloon catheter allows not only elevation of the orbital bone fragment but also expansion of the maxillary sinus in cases where there is a fracture of its walls. But postoperative follow-up method using computed tomography is expensive. Hence, we report a simple and inexpensive follow-up method using radiopaque dye inflation. Methods: We performed endoscopic transantral approach in 5 cases of blow-out fracture under general anesthesia. To accomplish this technique, a rigid 4 mm, 0 or 30 degree angled endoscopy was inserted into the maxillary sinus. Inflation of the catheter started gradually, with 10 to 15 mL of saline mixed radiopaque dye (saline: dye, 5 : 1) by syringe and while observing the elevation of the fracture site with endoscope until a proper contour was reached. For the maintain of the position of fractured site, 12 French urinary balloon foley catheter were used in fracture site for 7 - 10 days. Results: Postoperative assessment was performed by means of clinical and simple radiographic examination to secure the catheter under the inferior orbital wall and in the maxillary sinus. No specific complications occurred related to this procedure. Results of the surgery and follow-up in all cases were satisfactory. Conclusion: It may be a better alternative to the conventional follow-up method, with less cost and effectiveness of the catheter patency. The advantages of using the urinary balloon foley catheter with the radiopaque dye include the following : it is safe, efficacy, simple, and especially low cost. On drawback of this method is the discomfort to the patient caused by the catheter during the treatment.

• 한국임상수의학회지
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• 제23권2호
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• pp.175-179
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• 2006

3.16 kg의 몸무게를 가진, 7년령의 거세하지 않은 수컷 요크셔테리어견이 복부팽만, 설사, 체중감소로 내원하였다. 병력청취, 신체검사, 실험실검사, 방사선학적 검사, 초음파검사, 탐색적 개복술과 조직병리학적 검사 둥을 통해 최종적으로 intestinal lymphangiectasia로 진단되었다. 본 증례에서 나타난 증상과 여러 검사 결과들은 이전에 보고된 증상 및 결과와 대체로 일치하였으나, 저칼슘혈증과 저콜레스테롤혈증을 나타내지 않았다는 점에서 기존의 증례와 차이가 있었다. 처치법으로 프레드니솔론 (Prednisolone $Korns^(R)$, 한국 코러스, 충북, 한국, 2 mg/kg. 경구투여, 1일 2회)을 사용하였지만 효과가 없었다. 따라서, 아자싸이어프린 ($Azaprine^(R)$, 한국 유나이티드 제약, 충남, 한국, 1 mg/kg, 경구투 여, 1일 l회)을 추가하여 복용시킨 결과 임상증상이 개선되었다.

• 한국산업보건학회지
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• 제27권3호
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• pp.187-192
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• 2017

Objectives: It is difficult to identify exposure factors in the semiconductor industry due to low exposure levels to hazardous substances and because various processes take place in fabrication (FAB). Furthermore, a single worker often experiences a variety of job histories, so it is difficult to classify similar exposure groups (SEG) in the semiconductor industry. Therefore, we intend to develop a new exposure index, the period of working in FAB, that is applicable to the semiconductor industry. Methods: First, in specifying the classification of jobs, we clearly distinguished whether they were FAB workers or non-FAB workers. We checked FAB working hours per week through questionnaires administered to FAB workers. We derived an exposure index called FAB-Year that can represent the period of working in FAB. FAB-Year is an index that can quantitatively indicate the period of working in FAB, and one FAB-Year is defined as working in FAB for 40 hours per week for one year. Results: A total of 8,453 persons were surveyed, and male engineers and female operators occupied 90% of the total. The average total years of service of the subjects was 9.7 years, and the average FAB-Year value was 6.8. This means that the FAB-working ratio occupies 70% of total years of service. The average FAB-Year value for female operators was 8.4, for male facility engineers it was 7.7, and for male process engineers it was 3.5. A FAB-Year standardization value according to personal information (gender, job group, entry year, retirement year) for the survey subjects can be calculated, and standardized estimation values can be applied to workers who are not participating in the survey, such as retirees and workers on a leave of absence (LOA). Conclusions: This study suggests an alternative method for overcoming the limitations on epidemiological study of the semiconductor industry where it is difficult to classify exposure groups by developing a new exposure index called FAB-Year. Since FAB-Year is a quantitative index, we expect that various approaches will be possible in future epidemiological studies.

• 한국측량학회지
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• 제16권1호
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• pp.27-40
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• 1998

본 논문은 구조물의 변형과 거동의 모니터링에 GPS 측위기술의 적용가능성을 제시한다. 첫번째 부분은 GPS관측의 반복재현성(repeatability)에 대한 정량적 연구로서 기준망내 기준점들간의 기선벡터를 반복적으로 측정함에 있어서 동일한 결과가 얻어질 수 있는가를 구명하고자 하였으며, 두번째 부분은 한 점당 몇 분이내의 관측시간이 소요되는 kinematic GPS측량의 변형 모니터링 수행성을 평가하고자 하였다. 흙댐을 대상으로 계절 및 수위정도에 따라 총 4차에 걸쳐 모니터링 시험망에 대한 관측을 수행하였다. 기준망은 static 방식으로 관측하였고, 모니터링 점들에 대해서는 kinematic방식은 물론 static 및 rapid-static방식으로 각각 관측을 행하여 종래의 측량기법으로 얻어진 결과와 비교하였다. 기준점들간의 기선벡터 관측에서 평균 7 mm 이내의 3차원 기선벡터의 반복재현성을 성취할 수 있었으며, 기준망들의 폐합오차는 4∼27 ppm를 나타내었다. kinematic 접근법은 종래의 측량기법에 비하여 기준점들과 모니터링 점들간의 경사거리에서 34 m의 차를 나타내었고, 높이에서는 4∼8 mm의 차를 나타내었다. kinematic GPS 측량기법은 5 m정도의 측정능력을 지닌 종래의 측량기법에 의한 변형 모니터링의 효율적인 대안이 될 수 있음을 확인할 수 있었다.