• 한국조경학회지
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• 제30권1호
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• pp.75-86
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• 2002

This study was conducted to analyze the change of soil characteristics effect on the condition of urban forest in Sasang parti located near Sasang Industrial Complex. The results of this study are as follows; 1. Soil hardness is increasing from the area of forest, to the entrance, to facilities in that order. Soil acidity pH4.19∼4.23 in Sasang park indicated a high acidity condition. High levers of K, Na, Mg, Ca are shown in the areas composed of high soil hardness. 2. Pinus thunbergii in the overstory tree layer, Alnus japonica in the understory tree layer, and Rhus sylvestris in the shurb layer are shown respectively as dominant species based on the ground survey and the compution of important value. Pinus thunbergii is decreasing, while Alnus japonica and increasing. 3. Oplismenus undulatifolius which has a strong tolerance for air pollution, is shown as a dominant species of herbaceous plants in Sasang park. There are 10 species of Harbaceous in Sasang park compared to 20 species in Molundae park. This shows that deversity in herbaceous plants are imported by air pollution. 4. Species diversity indices of Sasang park is 0.8738∼0.9700 compared to 1.0817∼ 1.233 in Molundae park is due to the good condition of soil environment in addition to air pollution effects. 5. The vitality of Pinus thunbergii is 16.41∼20.42ER in Sasang park, and 12.42∼ 16.81ER, in Molundae park. This shows that tree vitality are impacted by soil characteristics. The regression analysis between tree vitality and soil environment shows the effects of is soil hardness, soil moisture, soil acidity, K, Na, Mg, Ca.

• Journal of Pharmaceutical Investigation
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• 제37권4호
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• pp.211-216
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• 2007

The bark of Alnus japonica has been used for the treatment of fever, hemorrhage and diarrehea in oriental traditional medicine. Recently, it was revealed that the diarylheptanoids from the bark of Alnus japonica possess anti-inflammatory activity and are expected to be applicable for atopic dermatitis. In this study, oregonin, one of major active components in the bark of Alnus japonica, was developed in the form of semisolid formulations for topical delivery. Oregonin was incorporated into four ointment bases: O/W cream, W/O cream, hydrophilic ointment and lipophilic ointment. Oregonin release from all formulation prepared was evaluated. Franz cell method and immersion method were employed to characterize the release patterns of drug from each formulation based on solvent availability. O/W cream showed a better release profile than the other formulations when evaluated with Franz cell method with an order of O/W cream, hydrophilic ointment, W/O cream and lipophilic ointment. In the immersion method, hydrophilic ointment showed the greatest release rate at times 1 hour exceeding compared to other bases with an order of hydrophilic ointment, O/W cream, W/O cream and lipophilic ointment. Hydrophilicity and solvent availability of formulation seems to significantly influence the release rate of oregonin from ointment bases. In this study, we successfully characterized the oregon in ointment and found that o/w cream is a promising formulation for the topical delivery of oregonin.

• 약학회지
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• 제56권2호
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• pp.99-107
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• 2012

The protective effect of AI-1367 (Alnus japonica extract) on liver injury was investigated. Primary rat hepatocyte intoxication was induced by tert-butyl hydroperoxide (tBH), carbon tetrachloride ($CCl_4$), or D-glactosamine (D-GalN). Liver injury was induced by $CCl_4$, D-GalN or MCD (methionine choline deficient)-diet in mouse. The cellular leakage of lactate dehyrogenase and cell viability followed by the treatment of hepatotoxicants were significantly improved by AI-1367 treatment at a concentration range of 5~50 ${\mu}g/ml$ for tBH, 5~50 ${\mu}g/ml$ for D-GalN, and 5~100 ${\mu}g/ml$ for $CCl_4$, respectively. Treatment with AI-1367 (20, 10, 5 mg/kg, p.o.) on liver injury induced by subcutaneous injection of $CCl_4$ or D-GalN reduced significantly the levels of aspartate transaminase and alanine transaminase in serum. Histological observations revealed that fatty acid changes, hepatocyte necrosis and inflammatory cell infiltration in $CCl_4$ (D-GalN)-induced liver injury was improved by administration of AI-1367. AI-1367 treatment (10, 5, 2.5 mg/kg, p.o.) also significantly recovered the body weight change and serum levels of aspartate transaminase, alanine transaminase and triglyceride in liver injury induced by MCD diet. From these results, AI-1367 shows protective effects against tBH, $CCl_4$, D-GalN, or MCD diet-induced hepatotoxicity in vitro or in vivo.

• Archives of Pharmacal Research
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• 제28권2호
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• pp.177-179
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• 2005

Nine known diarylheptanoids (1-9) isolated from the barks of Alnus japonica were evaluated for their inhibitory activities on nitric oxide (NO) and prostagrandin $E_2$ (COX-2) production in interferon-${\gamma}$ (INF-${\gamma}$) and lipopolysaccharide (LPS)-activated RAW 264.7 cells in vitro. The NO and COX-2 levels were moderately reduced by the addition of compounds (1-9). Among these compounds, compounds 6 and 8 inhibited NO production in a dose dependent manner with an $IC_{50}$ of 16.7 and $27.2\;{\mu}g/mL$, respectively (positive control, L-NMMA; $22.8\;{\mu}g/mL$), and compounds 6, 7, 8, and 9 reduced the COX-2 level in a dose dependent manner with an $IC_{50}$ of 20.7, 25.7, 25.0, and $27.3\;{\mu}g/mL$, respectively (positive control, indomethacin; $26.2\;{\mu}g/mL$). An analysis of the structural activity relationship among these diarylheptanoids suggests that the presence of a keto-enol group in the heptane moiety or a caffeoyl group in the aromatic ring were important for the efficacy on the inhibitory activities of NO and COX-2 production.

• 한국균학회지
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• 제26권2호통권85호
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• pp.265-274
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• 1998

목질진흙버섯(Phellinus sp.) 자실체의 인공재배를 위한 기초자료마련의 일환으로 자실체로부터 순수분리한 상황속의 한 균주를 이용하여 참나무 (Quercus aliena), 뽕나무(Morus alba) 및 오리나무(Alnus japonica)의 3종 톱밥기질에 따른 균사생육의 최적조건을 반응표면분석방법으로 조사하였다. 균사생육에 영향을 미치는 3종의 독립변수(쌀겨첨가량, 배지의 초기 pH 및 수분함량)와 그 최적값을 검토한 결과, 쌀겨첨가량은 3종의 톱밥배지 모두에서 약 30%(w/w) 첨가시에 가장 좋았으며, 수분함량은 $65{\sim}70%(w/v)$ 범위 에서 좋았다. 배지의 초기 pH는 참나무와 뽕나무에서는 $pH\;5{\sim}6$, 오리나무 톱밥배지의 경우는 pH 6에서 가장 좋았다. 균사의 생육온도는 모든 톱밥배지에서 $25{\sim}30^{\circ}C$의 온도범위에서 좋았으나, 톱밥수종에 따른 차이를 보였다. 균사생육에 영향을 미치는 쌀겨첨가량$(X_1)$, 배지의 초기 $pH(X_2)$ 및 수분함량 $(X_3)$을 각각 종속변수로 하고, 균사의 생육길이를 독립변수(Y)로 하여 반응 표면분석한 결과, 참나무 톱밥의 경우, 쌀겨첨가량 31.01%(w/w), 배지의 PH 5.31, 그리고 수분함량 69.03%(w/v)에서 얻어졌으며, 이때 기대되는 균사의 생육길이는 8.32cm이었다. 특히, 쌀겨 첨가량$(X_1)$과 수분함량$(X_3)$)의 회귀계수가 높아, 이 변수들이 균사생육에 큰 영향을 미침을 알 수 있었다. 뽕나무 톱밥배지의 경우, 쌀겨첨가량, 배지의 초기 pH 및 수분함량의 정상점은 각각 28.77%(w/w), 5.28및 69.8%(w/v)로, 예측되는 균사의 생육길이는 7.60 cm이었다. 오리나무 톱밥 배지의 정상점은 쌀겨첨가량 28.74%(w/w), 배지의 pH 6.04및 수분함량 66.96%(w/v)으로, 이때, 예상되는 균사의 생육길이는 5.38cm이었다. 이상의 결과로부터 목질진흙버섯의 균사 생육과 독립변수들 사이에는 상관관계가 높았고, 쌀겨첨가량과 배지의 초기 pH가 수분함량보다 균사생육에 더 큰 영향을 미쳤으며, 목질진흙버섯의 균사 생육을 위한 최적의 톱밥 수종은 참나무(Quercus aliena)>뽕나무(Morus alba)>오리나무(Alnus japonica)의 순이었다.

• Journal of Plant Biology
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• 제28권3호
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• pp.207-216
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• 1985

Radial immunodiffusion, rocket immunoelectrophoresis, and Boyden procedure have been employed as quantitative analysis of pollen proteins in a systematic investigation of selected amentiferous plant taxa. Data presented here are continued and supplementary to the previous qualitative analysis of immunoprecipitin systems for the same purpose. Although the number of taxa tested has been limited, the serological evidence indicates that the Betulaceae has the greatest similarity to the Fagaceae, next to the Juglandaceae, the least to the Salicaceae, when antisera against Alnus hirsuta and Betula platyphylla var. japonica were used for tests. Within the Betulaceae Alnus and Betula show greatly similar affinities together, but less similar to the rest of genera: Carpinus, Carya and Corylus. When antisera against Quercus aliena, Q. dentata, and Q. glauca were used for tests, the following decreasing order to serological affinities was obtained: Quercus Alnus, Betula Carpinus, Carya, Corylus Juglans, Pterocarya Populus. Overall serological data come closer to supportint the classification systems of Cronquist, Takhtajan, and Hutchinson; but less of Thorne and Bessey. In addition this investigation indicated that pollen, with its high protein content, provided an excellent source of extractable antigens for serosystematic researches.

• Journal of Plant Biology
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• 제28권3호
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• pp.179-190
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• 1985

A review of taxonomic treatments of the amentiferous plant taxa reveals diverse classification. It appears to be necessary to investigate new characteristics and attempt to determine the significance of these characteristics in terms of amentiferous systematics. Serological techniques have been empolyed in a systematic investigation of selected taxa of the Amentiferae: Betulaceae, Fagaceae, Juglandaceae, Myricaceae, and Salicaceae. Data by qualitative analysis of pollen proteins, double immunodiffusion and conventional immunoelectrophoresis, have proved to be valuable in delimitation of taxa tested. When the antisera against Alnus hirsuta, Betula platyphylla var. japonica, Quercus aliena, Q. dentata, Q. glauca were tested; Alnus and Betula have the greatest protein similarity to one another; and next to the rest of betulaceous genera. Relatively strong protein similarity obtained with most representatives of Quercus and Fagus when reacted with antisera against Alnus and Betula is very much in contrast to the weak protein similarity obtained for the genera of Juglandaceae, Myricaceae, particularly of Salicaceae Tested with the same antisera. When Quercus antisera were used for various genera, the weakest protein similarity was obtained with Populus.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.271.2-271.2
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• 2001

• 한국환경복원기술학회지
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• 제7권2호
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• pp.62-67
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• 2004

Forest community structure analysis in the Seooreung area, Goyang-si was carried out to supply basic data for reasonable management and conservation. The forest vegetation of the Seooreung area was classified into Alnus japonica community, Quercus mongolica - Carpinus laxiflora community, Quercus serrata community, and Pinus densiflora community. The dominant species in the area were in the order of Quercus mongolica, Quercus serrata, Robinia pseudoacacia, Quercus variabilis, Quercus aliena, Styrax japonica, Pinus rigida, and Alnus hirsuta. According to the DBH analysis result, Pinus densiflora was dominance species in Tomb area. Pinus rigida had a density of normal distribution style in the entire forests, therefore, their dominance are expected to continue for now. However, Quercus serrata is expected to extend their influence in the future in competition with Pinus rigida. Quercus mongolica had a density of normal distribution style in the Quercus mongolica - Carpinus laxiflora community, therefore, their dominance are expected to continue for now. Carpinus laxiflora is expected to extend their influence in the future in competition with Quercus mongolica. The relationship between the distribution of dominant species of forest vegetation and soil condition in Seooreung area was investigated by analyzing soil nutrition and moisture gradient. Alnus hirsuta community was distributed in the mesic and medium nutrition area and Quercus mongolica - Carpinus laxiflora community in the serix and relative good nutrition area. Pinus densiflora community was distributed in the serix and poor nutrition area and Quercus serrata community in the subserix and medium nutrition area.

• 한국자원식물학회지
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• 제37권2호
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• pp.120-129
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• 2024

활성산소종은 미토콘드리아 대사활동을 통해 생성되는 반응성이 강한 산소 함유 분자로서 특히 간에서 복잡한 세포 분자와 반응하여 산화적 스트레스를 유발하는 물질이다(Choi et al., 2012). 에탄올 대사 과정에서는 ROS가 생성되어 산화스트레스를 유발하며, 과도한 에탄올은 ROS를 제거하는 항산화 시스템을 억제함으로써 지방, 단백질 또는 DNA와 같은 생체 분자에 산화 손상을 유발하여 세포를 손상시킨다(Albano, 2006; Koch et al., 2004). 본 연구에서는 오리나무 잎 추출물(AJL)에서 알코올(EtOH)로 유발된 산화적 스트레스에 대한 간세포 손상의 보호 효과를 평가하기 위해 HepG2/2E1 세포에서 EtOH와 AJL 및 대조군silymarin을 각각 처리한 후 세포생존율을 평가하고 세포독성이 없는 범위를 설정하여 활성산소종 생성량, 항산화 관련 지표(DPPH, ABTS, MDA, GSH) 및 간 손상 지표(AST 및 ALT)를 측정하였다. 알코올에 대한 간 보호 효과에서는 알코올만 처리한 군의 세포생존율이 53.8%로 감소하였으나 AJL을 함께 처리 군에서 세포생존율이 85.1%의 유의적인 증가로 간 보호효과를 확인하였다. 다음으로 AJL에서 세포 내 ROS 생성 억제능은 에탄올 처리군에서 정상군에 비해 유의적으로 ROS 생성이 증가하였으나 AJL 농도별 처리시 정상군 수준으로 ROS 생성을 현저하게 감소시켰다. MDA 함량은 에탄올 처리군에서 무처리군에 비해 유의적으로 증가하였으나 AJL 처리시 농도 의존적으로 에탄올 처리군에 비해 유의적인 차이를 보이며 MDA 함량을 감소시켰다. GSH 함량은 에탄올 처리군에서 정상군보다 유의적으로 함량이 감소하였으나 AJL 농도별 처리군 모두 에탄올 처리군에 비해 GSH 함량을 증가시켰다. 또한 간손상 지표로 활용되는 AST 및 ALT 활성 변화 확인 시, 에탄올 처리에 따른 간세포 손상군에 비하여 AJL 처리군에서는 농도 의존적으로 현저하게 감소시켰으며, 대조군silymarin과 비교하여 유사한 감소된 결과를 관찰하였다. 이상의 결과로부터 종합해 볼때AJL은 간손상에 대한 보호 및 간손상 억제 가능성을 기대할 수 있으며, 간기능 개선 효과에 대한 건강기능식품개발의 기초자료로서 활용될 수 있을 것이라 사료된다.