• Korean Journal of Microbiology
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• v.21 no.4
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• pp.185-190
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• 1983

Twelve Microorganisms capable of utilizing diaminododecane were isolated from the soil by enrichment culture technique. Seven strains of these were identified as Corynebacterium. The isolated strains were tested for the ability to utilize as carbon source, 10 different kind of alkane derivatives containing CN, $NH_2$, Cl, and SH groups. Laurylcyanide, dicyanooetane, chlorodecane, and dichlorodecane were not utilized by any of the isolated strains; putrescine dihydrochloride, cadaverine dihydrochloride, diaminododecane, and n-dodecane were utilized by all of the isolated strains; and all of the isolated strains except DAD 2-3 could utilize dodecylmercaptan. The alkane derivatives that did not serve as ,growth substrates were tested further in oxidation tests using resting cell preparation. Alkane derivatives that are being oxidized by all of the isolated strains are laurylcyanide and dichlorodecane. Dicyanooctane was also oxidized by all of the isolated strains except DAD 30L, chlorodecane was the only oxidized by the three isolated strains. The most remarkable substrate that is being oxidized is dichlorodecane containing CN groups diterminally. Evidence obtained with thin layer chromatography of ,ethyl acetate extracts of culture broth of isolated strains grown in some alkane derivatives shows that these alkane derivatives are degraded.

• Journal of the Korean Chemical Society
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• v.13 no.2
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• pp.187-193
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• 1969

As part of an extensive program on the microbiological oxidation of hydrocarbons, reaction conditions and nutrients consumption of candida lipolytica grown on alkane as carbon source were studied. For optimum growth of yeast, the conditions of pH 5, temperature $30^{\circ}C$, carbon number $C_{16}$& $C_{18}$aeration 25.6 1/l/hr, agitation 3000 r.p.m., hydrocarbon concentration 10% were obtained. Carbon and nitrogen balance related to these conditions were also investigated. In the course of this investigation, some significant effects of pH and carbon number of hydrocarbon variation were observed.

• Korean Journal of Microbiology
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• v.32 no.2
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• pp.172-175
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• 1994

Xanthomonas campestris M12 carrying OCT plasmid which could dissimilate octane was able to utilize n-alkanes of eight to sixteen carbon atoms via the capacity of this plasmid. M12 strain could utilize terminal oxidation products of these primary, alkanes, alcohols, aldehydes and fatty acids but not hexanoic acid, adipic acid, pimelic acid and heptanal. This strain also biodegraded n-alkanes by monoterminal or diterminal oxdation of straight-chain fatty acids, and branched-chain alkane.

• Bulletin of the Korean Chemical Society
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• v.24 no.12
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• pp.1835-1837
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• 2003

• Bulletin of the Korean Chemical Society
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• v.18 no.4
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• pp.362-363
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• 1997

• Journal of Environmental Health Sciences
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• v.11 no.2
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• pp.65-75
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• 1985

Microorganisms capable of utilizing diaminododecane containing amine groups diterminally were isolated from the soil by enrichment culture. One strain of these isolated strain, designated as EL-0112L, was selected for this study. The results of this study were as follows. 1. This isolated strain EL-0112L was identified as Microbacterium, from the results of morphological, cultural, and biochemical tests. This isolated strain was named temporarily Microbacterium sp. EL-0112L for convenience. 2. Microbacterium sp. EL-0112L was tested for ability to utilize different kinds of substitued alkanes containing cyan, amine, chloro, and thiol groups(monoterminally or diterminall substituted) as carbon source. Pentamethylenediamine, hexamethylenediamine, n-decane, laurylamine, and alkane derivatives containing cyan, chloro, and thiol groups were not utilized by Microbacterium sp. EL-0112L. 3. The alkane derivatives that did not serve as growth substrates were tested further in oxidation tests using resting cell preparation of Microbacterium sp. EL-0112 L. Alkane derivatives containing cyan, chloro, thiol groups, and n-decane were oxidized by Microbacterium sp. EL-0112 L. It is possible that this isolated strain is also able to degrade their substituted counterparts since they are structually similar to diaminododecane. The remarkable substrates that were being oxidized were dichlorodecane, and 1-dodecanethiol. Microbacterium sp. EL- 0112L could not oxidize pentamethylenediamine, and hexamethylenediamine. 4. The metabolic products formed from diaminododecane by Microbacterium sp. EL-0112 L were acid compound containing carboxyl group and not containing amine group. On the thin layer chromatography, Rf values of these metabolic products were different from that of the product formed by Corynebacterium sp. EL-0112L. These results suggested the specificity of diaminododecane as carbon source.

• Microbiology and Biotechnology Letters
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• v.10 no.2
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• pp.109-115
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• 1982

A Corynebacterium sp. capable of utilizing diaminododecane (DAD) were isolated from the soil by enrichment culture. Among 9 different kinds of substituted alkanes containing CN, NH$_2$, Cl, and SH groups (monoteminally or diterminally substituted) tested as carbon source, the isolate, designated as DAD 2-2. utilized DAD, putrescine dihydrochloride, dodecanethiol, dodecane and lautylamine. Thioanisole, decanedithiol, dicyanooctane, laurylcyanide, and dichlorodecane were not utilized. When emulgen 950 was added to the medium, the growth of DAD 2-2 was greatly accelerated. Isolated DAD 2-2 grown in the medium with DAD as carbon source formed ethyl $\alpha$-ketoglutarate. Metabolic product of DAD 2-2 grown in a medium without nitrogen source was different from that of grown in a medium with NH$_4$NO$_3$. When glucose, putrescine, n-dodecane and other alkane derivatives were tested in place of DAD, isolate DAD 2-2 yielded products different from those they formed with DAD suggesting specificity of DAD as a carbon source.

• Bulletin of the Korean Chemical Society
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• v.17 no.7
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• pp.625-630
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• 1996

The cobalt-substituted polyoxotungstate [(CoPW11O39)5-] has been used as a catalyst in olefin epoxidation and alkane hydroxylation reactions. The epoxidation of olefins by iodosylbenzene in CH3CN yielded epoxides predominantly with trace amounts of allylic oxidation products. cis-Stilbene was streoselectively oxidized to cis-stilbene oxide with small amounts of trans-stilbene oxide and benzaldehyde formation. The epoxidation of carbamazepine (CBZ) by potassium monopersulfate in aqueous solution gave the corresponding CBZ 10,11-oxide product. Other transition metal-substituted polyoxotungstates (M=Mn2+, Fe2+, Ni2+, and Cu2+) were inactive in the CBZ epoxidation reaction. The cobalt-substituted polyoxotungstate also catalyzed the oxidation of alkanes with m-chloroperbenzoic acid to give the corresponding alcohols and ketones. The presence of CH2Br2 in the hydroxylation of cyclohexane afforded the formation of bromocyclohexane, suggesting the participation of cyclohexyl radical. In the 18O-labeled water experiment, there was no incorporation of 18O into the cyclohexanol product when the hydroxylation of cyclohexane by MCPBA was carried out in the presence of H218O. Some mechanistic aspects are discussed as well.

• Journal of ILASS-Korea
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• v.23 no.4
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• pp.175-184
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• 2018

Emission characteristics of regulated (NOx, PM, CO, NMHC) and unregulated (VOCs, aldehydes, PAHs) air pollutants were investigated for diesel heavy duty buses equipped with different after-treatment systems (DPF+EGR and SCR) under urban driving cycle. The combustion temperature and the working temperature of SCR catalysts were important to make impact on NOx emissions, whereas PM emissions were low. The alkane groups dominated NMVOCs emissions, making 42.6~59.4% of sum of the NMVOCs emissions. Especially, alkane emissions of DPF+EGR-equipped vehicle included DOC had 14.9~15.5% higher than those of SCR-equipped vehicle due to low efficiency of oxidation catalyst. In the case of individual NMVOCs, n-nonane and propylene emissions highly occupied for DPF+EGR and SCR, respectively. Formaldehyde emissions among aldehydes were the highest and PAHs emissions were hardly detected except naphthalene and phenanthrene. The NMHC speciation has been shown to be the highest of the formaldehyde ranged 20.8~21.5%. The results of this study will be contributed to establish Korean HAPs emission inventory for automobile source.

• Journal of the Korean Chemical Society
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• v.36 no.5
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• pp.744-752
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• 1992

Polymer bonded metalloporphyrins are synthesized by reaction between Fe(III) protoporphyrin or Mn(II) tetrakis(4-N-carboxyphenyl)porphyrin with polystyrene divinylbenzene copolymer. The spectroscopic properties of synthetic polymer bonded metalloporphyrins are investigated by using resonance Raman spectrometer. By synthetic polymer bonded metalloporphyrins as catalyst, which are model of cytochrome P-450 and peroxidases, epoxidation of olefins and oxidation of alkanes are achieved with H2O2 as oxidant. The catalytic efficiencies with polymer bonded metalloporphyrins are improved on that with corresponding nonpolymer bonded metalloporphyrins. Especially those can be reused because of stability against oxidant. Electron donating imidazole derivatives, which are attached in 5th position of central metal of metalloporphyrins, enhance the catalytic efficiencies.