• The Journal of Internal Korean Medicine
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• 제29권1호
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• pp.265-277
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• 2008

In order to investigate the protective effects of Gami Yugan-tang on liver damage in rats induced by $CCl_{4}$ and d-galactosamine, the serum transaminase(ALT & AST), alkaline phosphatase(ALP), lactic dehydrogenase(LDH), superoxide dismutase(SOD), catalase, glutathione S-transferase(GST), glutathione peroxidase(GPX) for enzyme activities, and lipid peroxidation were measured. All animals were divided into 4 groups: normal group (untreated), control group (treated with 0.9% saline solution), sample I group (treated with 740mg/kg Gami Yugan-tang), and sample II group (treated with 1,480mg/kg Gami Yugan-tang). The results were as follows : 1. The results of liver damage in rats induced by $CCl_4$ : The protective effects of ALT were displayed in sample I and sample II, and AST, ALP, LDH, SOD, catalase, GST, GPX, and lipid peroxidation were noted in sample II group. It showed slight necrosis of hepatic cell and pathologic changes, for example, inflammatory cells infiltration were improved in sample II group compared to the control group. 2. The results of liver damage in rats induced by d-galactosamine : The inhibitory effects of AST, ALT, LDH, and ALP activities were noted in both sample I and sample II groups. The findings from this experiment suggests that Gami Yugan-tang has protective effects against liver damage in rats induced by $CCl_{4}$ and d-galactosamine.

• Applied Biological Chemistry
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• 제13권1호
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• pp.65-72
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• 1970

Two peptides (Im pr-M, Im ch-M) derived from Im ${\lambda}-type$ of Bence Jones Protein and one peptide (Ikch-M) from Ik were separated and purified using the Dowex $50{\times}2$ column $(1{\times}20\;cm)$ and Dowex $1{\times}2(0.9{\times}50\;cm)$. The buffer solution was composed of 1% pyridine and IM formic acid in Dowex $1{\times}2$ column. The blocked N-terminal was examined with ninhydrin reaction before and after alkaline hydrolysis, which was fractionated by Dowex $1{\times}2$ column. Pyrro-glutamic acid in N-terminal residue was identified by comparing with the authentic pyrro-glutamic acid through a high voltage electrophoresis (pH 3.5, 3000 V.) after the peptide Im pr-M (PCA. Ser) was cleavaged at the position of serine with cone. (12 N) HCl and the pyrro-glutamic acid was converted to glutamic acid by treating it with N-NaOH for 116 hours at $27^{\circ}C$. The substractive method was applied to find out the sequence of peptides and carboxypeptidase A was employed to release C-terminal residue from the peptide. In present study PCA. Ser in Im Pr-M was isolated from the pronase digested ${\lambda}$-type Bence Jones protein. The yield of the Im Pr-M was 79.6 percent of its theoretical value, based on the molecular weight of Bence Jones Protein. Im ch-M (PCA. Ser Val. Leu) was isolated from the chymotrypsin digested ${\lambda}$-type Bence Jones Protein. The yield of the Im ch-M was 72.2 percent. based on the molecular weight of Bence Jones Protein. Ik ch-M (PCA. Ser. Ala. Leu) was isolated from the chymotrypsin digested ${\lambda}$-type Bence Jones Protein and its yield was 42% based on the molecular weight of Bence Jones Protein.

• Analytical Science and Technology
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• 제13권5호
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• pp.659-665
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• 2000

The changes in $UV_{254}$ and concentrations of $H_2O_2$ formed by ozonation of aqueous humic acid in ozone/high pH, peroxone process and in the presence of radical scavenger, $HCO_3{^-}$ were investigated. This study confirmed that the formation of $H_2O_2$ by ozonation may undergo different reaction pathways compared to those of $UV_{254}$ reduction in the degradation of the humic acid. The concentration of $H_2O_2$ produced by ozonation was found to be increased with decreasing pH of the sample solution due to the higher stability of ozone molecules at acidic conditions. On the while, $UV_{254}$ reduction was found to be higher at alkaline conditions or larger amount of $H_2O_2$ additions as a radical promoter in which the producing of ${\cdot}OH$, ${\cdot}HO_2$ radicals can be more favorable. From the results, it has been suggested that the formation of $H_2O_2$ by ozonation depends mainly on the direct reactions of ozone with humic acid molecules, while $UV_{254}$ reduction is affected by both the indirect reactions of the radicals and direct reactions of ozone with humic acid.

• Analytical Science and Technology
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• 제31권1호
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• pp.47-56
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• 2018

Finding the blood left at a crime scene is very important to reconstruct or solve a criminal case. Although numerous reagents have been developed for use at crime scenes, luminol is the most representative. Bluestar Forensic has been used in recent years, but is expensive and cannot be stored after preparation. This study aims to develop a new luminol reagent that can be stored for a long period of time while maintaining the chemiluminescence intensity at the level of Bluestar Forensic. Because luminol dissolves well in aqueous alkaline solutions, the use of sodium hydroxide in the preparation of luminol reagents can promote the decomposition of hydrogen peroxide. Magnesium sulfate, sodium silicate, and potassium triphosphate have been used as hydrogen peroxide stabilizers. The effects of the addition of these substances on the chemiluminescence emission intensity and the storage period of the luminol reagents were confirmed. The addition of a hydrogen peroxide stabilizer was shown to have no significant affect on the chemiluminescence emissions intensity or stabilized pH of the luminol reagent during storage. It also greatly increases the shelf life of the reagents. The use of magnesium sulfate as a hydrogen peroxide stabilizer is the most appropriate. When sodium perborate is used instead of hydrogen peroxide as an oxidizing agent, there is no significant change in the sensitivity and chemiluminescence emissions intensity, but the storage period is shortened. However, after the reaction with blood, the pH of the mixed solution does not increase significantly, and is judged to be more suitable than a reagent made of hydrogen peroxide.

• Applied Biological Chemistry
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• 제34권4호
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• pp.373-378
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• 1991

The skin and fleshy substance on the net fiber of sponge-gourd fruit pressed mechanically was removed with 0.2% NaOH solution in $3{\sim}5$ hours. The treatment of 0.2% NaOH with 0.02% Monopol(non-ionogenic polyoxethylen derivative) as surfactnat and with 0.1% $Ca(OCl)_2$ as bleaching agent enhanced the effect to remove the fleshy substance and improve the quality of net fiber. Also, the wet hardness and tensile strength of net fiber were controlled by the crosslinkage of the fiber with glu tardialdehyde, glyoxal, and formalin, respectively. The net fiber was stable on the acid and alkaline solutions. Also the range of temperature to degradate the fiber was $338{\sim}385^{\circ}C$. These values indicated a fair stability. The improved net fiber can be used for raw material of bath, dish washing, oil and gas filter, and many kinds of decorations.

• The Korean Journal of Mycology
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• 제37권1호
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• pp.91-95
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• 2009

To examine physico-chemical properties of the polysaccharide extracted from liquid-cultured mycelia of Schizophyllum commune, each the polysaccharide was extracted with hot water treatment and then fractionated with ethanol, alkaline solution and ultrafiltration. And we determined carbohydrate contents, composition of amino acids, infra-red spectrum and viscosity. Carbohydrate contents of polysaccharide treated with ethanol and ultrafiltration were 72.0% and 62.3%, and proteins content were 15.3% and 32.0% respectively. The carbohydrate consisted of four monosaccharides and the protein contained 16 amino acids. The polysaccharide obtained from ultrafiltration was shown an absorption band characteristic of the ${\beta}$-glycosidic linkage by infra red spectra. These results suggest that the polysaccharide extracted from Schizophyllum commune showed the characteristics of proteinbounded polysaccharide, and it was ${\beta}$-glycosidic linkage with strong viscosity.

• Journal of the Mineralogical Society of Korea
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• 제18권3호
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• pp.183-194
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• 2005

The mineralogical, physicochemical and thermal properties of the Myogi bentonite occurring in Japan were measured. A adsorption properties of U, Th, Ce and Eu ions on the Myogi bentonite were also investigated in different solution concentrations and pH conditions. The Myogi bentonite showed a strong alkaline character (pH 10.4), very high swelling, viscosity property and CEC, and a slow flocculation behavior due to the strong hydrophilic property. By the thermal analysis, the dehydroxylation of crystal water in bulk and clay fractions of the Myogi bentonite occur at $591^{\circ}C$ and $658^{\circ}C$, respectively, The adsorption experiments of ions such as U, Th, Ce and Eu were conducted for 0.2 g bentonites with 20mL solutions of various concentrations and different pH conditions with pH 3, 5, 7, 9, and 11. As a result, the Myogi bentonite showed excellent adsorption capacities for Ce, Th and Eu ions, whereas U ion showed very low adsorption capacity. Generally, Ce, Th and Eu ions showed the similar adsorption properties for the different concentrated solutions and pH conditions. These adsorption properties seem to be affected by the formation of various forms of chemical species and precipitation as well as ionic exchange reaction and surface adsorptions on smectite. Some associated zeolite minerals perhaps have some effects on the adsorption of U, Th, Ce and Eu on Myogi bentonite.

• Applied Biological Chemistry
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• 제41권5호
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• pp.399-404
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• 1998

Genipin was obtained from hydrolysis of geniposide isolated from gardenia fruits with ${\beta}-glucosidase$. Reaction of genipin with glycine, alanine, histidine, lysine, phenylalanine and glutamate in aqueous buffer solution converted colorless starting materials to blue pigments. Effect of pH for the formation of blue pigments was tested using UV/Vis spectrophotometer. The optimum pH for the formation of blue pigments was 7.0. No pigment and trace amounts were formed at acidic (pH 3.0) and alkaline (pH 12.0) conditions, respectively. The amount and tincture of blue color were distinct with different amino acids. In contrast with lysine $({\lambda}_{max}=573\;nm)$, glycine $({\lambda}_{max}=595\;nm)$, phenylalanine $({\lambda}_{max}=602\;nm)$ and alanine $({\lambda}_{max}=595\;nm)$, the reaction of genipin with histidine $({\lambda}_{max}=601\;nm)$ and glutamate $({\lambda}_{max}=601\;nm)$ produced relatively small amounts of blue pigments. Rate constants for the formation of blue pigments from genipin with amino acids at various temperatures $(60,\;70,\;80,\;90^{\circ}C,\;pH\;7.0\;phosphate\;buffer)$ were obtained. Rate constants of genipin with basic amino acids were larger than neutral or acidic amino acids. Arrhenius activation energies of the formation of blue pigments indicated that activation energy of glycine $(E_A=9.8\;kcal/mol)$ was especially lower than those of other amino acids $(E_A=13.3{\sim}15.4\;kcal/mol)$.

• Microbiology and Biotechnology Letters
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• 제3권2호
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• pp.63-68
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• 1975

A biological active substance was isolated from the cultured medium of Streptomyces sp. and its biochemical characteristics were investigated. Isolation process of the substance was as follows; the pH of filterate of the cultured medium was adjusted to 3.0 with N-hydrochloric acid and saturated with sodium chloride, then chloroform was added to this filterate in one fifth portions and stirred vigorously. After extracting the active substance with chloroform in 3 stages, the chloroform layer combined and evaporatea after dehydrating with sodium sulfate. The substance was found to be to be toxic to various fresh water fishes; the lethal dose for an average size Pseudorasbora parva T. et. S. was 50ug per ml. In the acidic condition, the toxicity of the substance remained fora long time, while in the alkaline state, the toxicity was decreased very fast. This substance was found to be stable to organic solvents, but labile to heat treatment. The maximal revival time of Pseudorasbora parva T. et. S. was about 20 minutes in 25 ug/ml of the substance solution.

• Journal of the korean academy of Pediatric Dentistry
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• 제29권3호
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• pp.430-438
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• 2002

The aim of this study was to evaluate the resistance to degradation of four commercial composite resins-Prodigy(Kerr, USA), Vitalescence(Ultradent, USA), Z 250(3M, USA), Filtek flow(3M, USA)- in an alkaline solution. Resistance to degradation was evaluated on the basis of following parameters: (a) mass loss(%), (b) Si loss(ppm), (c) degradation depth($\mu}m$). The results were as follows: 1. There was no significant difference between Prodigy and Vitalescence, also Z 250 and Filtek flow. But, there was significant difference between former group and latter group. 2. The sequence of the degree of degradation layer depth was in descending order by Z 250, Filtek flow, Prodigy, Vitalescence. There was significant difference among the materials. 3. The sequence of the Si loss was in descending order by Filtek flow, Z 250, Prodigy, Vitalescence. There was significant difference among the materials. 4. The correlation coefficient between mass loss and degradation layer depth(r=0.714, p<0.05), mass loss and Si loss(r=0.770, p<0.05), and degradation layer depth and Si loss(r=0.930, p<0.05) were relatively high. 5. When observed with SEM, destruction of bonding was observed between resin matrix and filler. 6. When observed with CLSM, degradation layer depth of composite resin surface was observed.