• Title/Summary/Keyword: Alkali

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In-Vitro Development of Early Stage Mouse and Bovine Embryos to Blastocysts in TCM 199 Supplemented with nonspecific Immunostimulator $Barodon-FX^{(R)}$ (비특이 면역증강제 $Barodon-FX^{(R)}$ 첨가 TCM199에서 생쥐 및 소 초기배의 체외 배반포 발달에 관한 연구)

  • 정영채;나광빈;김창근;류재원;최수일;전경수;류범룡
    • Korean Journal of Animal Reproduction
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    • v.25 no.2
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    • pp.131-138
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    • 2001
  • This experiment was designed to evaluate effects of nonspecific immunostimulator(NIS) Barodon-FX(equation omitted), anionic alkali mineral complex and far-infrared radiation solution on in vivo-produced mouse and in vitro-produced bovine embryos to blastocyst development. Proportion of mouse embryos developing into blastocyst was not greater in BSA- and Barodon-added medium than in BSA-control, but there was signifcantly different(P < 0.05) in hatching and hatched blastocyst development between 0.25% Barodon-and PVP-contained medium(54.7%) than PVP-control(32.5%). BOEC and GC resulted in higher proliferation rate(24∼40% and 17∼22%, respectively) in 0.25∼0.5% Barodon-added medium than in controls, but proliferation of GC and CC greatly decreased in 1∼2% Barodon-added medium. Effect of Barodon on cell proliferation greatly varied among somatic cells. Proportion of early bovine embryos developing into morula and blastocyst was significantly greater(P < 0.05) in 0.5% Barodon-added medium(50% and 63.6%) than in control(31.6% and 27.4%) under co-culture with BOEC and GC, but developmental rate was not different between other Barodon treatments and control. These data indicate that effect of Barodon on cell proliferation significantly varied between somatic cells and that addition of 0.5% Barodon in BOEC-coculture system may further improve blastocyst development in early bovine embryos.

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Study on the Damage Mechanism by Salt of White Porcelain Figurine in Underglaze Iron (백자 철화 인물형 명기의 염 손상 메커니즘 연구)

  • Lee, Sun Myung;Jin, Hong Ju;Yun, Ji Hyeon;Kwon, Oh Young
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.368-382
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    • 2020
  • It was confirmed that a white porcelain figurine in underglaze iron was damaged after exhibition. This study analyzes the current state of salt damage on the artifact and identifies the factors contributing to its deterioration by examining the material characteristics of the artifact and exhibition environment. The analysis will thus assist in preparing a conservation scheme for artifacts. The crystallized carbonate on the surface of the white porcelain figurine is a water-soluble alkali salt with high hygroscopicity and high solubility in water. This solubility increases as the temperature increases. The figurine was low-fired at approximately 1000℃. A lead glaze was applied, and thin cracks were formed on the glazed surface, indicating poor surface properties. Our analysis suggested that the showcase used in the exhibition likely created a moist environment resulting from condensation, as it was exposed to high temperature and relative humidity, particularly in comparison to the exhibition room where the temperature was regulated using an air conditioner. In addition, the artifacts in the showcase were exposed to sudden changes in temperature and relative humidity as the air conditioner was repeatedly turned on and off. Therefore, it can be deduced that the soluble salt remaining on the white porcelain figurine moved toward the surface of the relatively weak glaze as a result of the temperature, and the crystallized salt exacerbated surface damage as the moisture evaporated in a dry environment.

A Study on the Silica Removal in Primary System using the Membrane Process (막 분리 공정을 이용한 1차 계통 실리카 제거에 관한 연구)

  • Kim Bong-Jin;Lee Sang-Jin;Yang Ho-Yeon;Kim Kyung-Duk;Jung Hee-Chul;Jo Hang-Rae
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.06a
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    • pp.137-144
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    • 2005
  • Silica in primary system combines with an alkali grammatical particle metal and forms the zeolite layer which is hindering the heat transfer on the surface of the cladding. Zeolite layer becomes the cause of the damage in this way. The problems of the NPP's primary system have been issued steadily by EPRI. Through a series of experiments of the laboratory scale, we confirmed the applicability of NF membrane for silica removal, as silica rejection rate of NF membrane is about $60\;{\sim}\;70\%$ and boron rejection rate is about $10\;{\sim}\;20\%$. We accomplished a site experiment about four NF membranes manufactured by FilmTec and Osmonics Inc. In experiment using 400L of SFP water, when operation pressure is $10kg_{f}/cm^2$, we confirmed that the silica rejection rate of NF90-2540 manufactured by FilmTec Inc. is about $98\%$, boron rejection rate is about $43\%$. The silica rejection rate of NF270-2540 is about $38\%$, boron rejection rate is about $3.5\%$. Afterward, through additional experiments, such as long term characteristic experiments, we are going to design a optimum NF membrane system for silica removal.

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Comparison of Rice Yield and Grain Quality Characteristics between Mature and Reclaimed Paddy Fields in Honam Plain Area (호남평야지 숙답과 간척답의 수량 및 미질 특성 비교)

  • 정진일;이선용;김종호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.3
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    • pp.382-390
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    • 1995
  • To compare the rice yield and grain quality characteristics between mature(MPF) and reclaimed paddy field(RPF), grain yield and its components, some physicochemical properties of rice grain and palatability of cooked rice were investigated for Dongjinbyeo grown at three different locations of mature and reclaimed paddy field, respectively, in south western part of Korea. Variation of culm length in mature paddy area was larger than that in reclaimed saline area. Panicle number per m$^2$ in RPF was more then that in MPF but grain number per m$^2$, filled grain ratio and 1, 000-grain weight in RPF were lower than those in MPT. Average yield of milled rice in RPF was 12% lower than that in MPF (5.l1t /ha). Daejeon showed the highest yielding among MPF and Yeongam reveald the highest grain production among RPF. RPF rices had slightly higher carbohydrate content and alkali digestibility but lower protein, fat and amylose content compared with MPF rices. RPF rices also exhibited higher mineral contents, Mg/K ratio, peak and hot viscosity compared with MPF rices. RPF rice showed slightly poor apparent grain quality but a little better eating quality of cooked rice compared with MPF rice.

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Studies on the Ripening Characteristics, Germination Speed and Quality among Different Floret of Japonica and Tongil Type of Rice under Different Growing Season (수도재배시기별 Japonica 및 Tongil형 품종의 영화간 등숙특성 발아세 및 미질연구)

  • 권규칠;박성규
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.310-323
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    • 1989
  • This study was carried out to find out the difference of ripening and growth characteristics among the different florets in same panicle. There were 2 different transplanting date (May 25 and June 10) and 6 varieties were used. Physiological ripening period, ripening speed, 1000 grain weight, germination speed and grain quality were studied among the different florets (1, 5 and 8 days after heading) in same panicle. Physiological ripening period was long in late transplanting, late flowering floret, and late varieties by 3-9 days in Japonica. Ripening speed and 1000 grain weight were high in early transplanting and early flowering florets. There was positive correlation among the ripening speed, 1000 grain weight and a average temperature but negative correlation between the ripening and physiological ripening period. Germination speed was excellent in early flowering floret after heading. There was difference in amylose content within varieties and different flowering grains and there was significant correlation between grain quality and meteorological factors in some varieties.

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A Preliminary Survey Result of Cu Occurrence in Tsogttsetsii Area, Mongolia (몽골 촉트체치 지역 동 산출지 예비조사결과)

  • Kim, In Joon;Lee, Jae Ho;Ryoo, Chung-Ryul;Lee, Bum-Han;Jin, Kwang Min;Davaasuren, Otgon-Erdene;Heo, Chul-Ho;Nam, Hyeong Tae
    • Economic and Environmental Geology
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    • v.50 no.4
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    • pp.313-324
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    • 2017
  • Tsogttsetsii area, an intrusive complex associated with Cu mineralization, is located in the South Gobi, Mongolia. We performed the cross geochemical and extended exploration survey in Tsogttsetsii area. Cu mineralization in Tsogttsetsii area is porphyry Cu type related with alkali granite intruded in Late Carboniferous to Early Permian. In the concentrated occurring to malachite appears extensively prophylitic alteration zone having a chlorite and epidote. As results of the survey, Cu contents of potable XRF and of chemical composition for altered rocks ranges 1.08 to 18.3% in the 30 points and 1.08 to 32.9% in the 13 points, respectively. Ore minerals identified in XRD analysis and polarizing microscope that samples of copper oxides were composed mainly of malachite, azurite, permingeatite and cuprite and the other minerals are pyrite, chalcopyrite, pyrargyrite, dickite, calcite, chlorite and epidote. Mineralization can be considered occurring to selectively some granite of the surrounding aplite and faults in the only upper part coming up the hydrothermal solution of the remaining residual magma after the aplite intrusion.

Evaluation of DNA Damage by Mercury Chloride (II) and Ionizing Radiation in HeLa Cells (이온화 방사선 및 염화수은(II)에 의한 자궁경부암 세포의 DNA 손상 평가)

  • Woo Hyun-Jung;Kim Ji-Hyang;Antonina Cebulska-Wasilewska;Kim Jin-Kyu
    • Korean Journal of Environmental Biology
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    • v.24 no.1 s.61
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    • pp.46-52
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    • 2006
  • The mercury is among the most highly bioconcentrated toxic trace metals. Many national and international agencies and organisations have targeted mercury for the possible emission control. The mercury toxicity depends on its chemical form, among which alkylmercury compounds are the most toxic. A human cervix uterus cancer cell line HeLa cells was employed to investigate the effect of the toxic heavy metal mercury (Hg) and ionizing radiation. In the in vitro comet assays for the genotoxicity in the HeLa cells, the group of Hg treatment after irradiation showed higher DNA breakage than the other groups. The tail extent moment and olive tail moment of the control group were $4.88{\pm}1.00\;and\;3.50{\pm}0.52$ while the values of the only Hg treatment group were $26.90{\pm}2.67\;and\;13.16{\pm}1.82$, respectively. The tail extent moment and olive tail moment of the only 0.001, 0.005, 0.01 Hg group were $12.24{\pm}1.82,\;8.20{\pm}2.15,\;20.30{\pm}1.30,\;12.26{\pm}0.52,\;40.65{\pm}2.94\;and \;20.38{\pm}1.49$, respectively. In the case of Hg treatment after irradiation, the tail extent moment and olive tail moment of the 0.001, 0.005, 0.01 Hg group were $56.50{\pm}3.93,\;32.69{\pm}2.48,\;62.03{\pm}5.14,\;31.56{\pm}1.97,\;72.73{\pm}3.70\;and \;39.44{\pm}3.23$, respectively. The results showed that Hg induced DNA single-strand breaks or alkali labile sites as assessed by the Comet assay. It is in good agreement with the reported results. The mercury inhibits the repair of DNA. The bacterial formamidopyrimidine-DNA glycosylase (Epg protein) recognizes and removes some oxidative DNA base modifications. Enzyme inactivation by Hg (II) may therefore be due either to interactions with rysteine residues outside the metal binding domain or to very high-affinity binding of Hg (II) which readily removes Zn (II) from the zinc finger.

A Study of Catalysts for Hydrogen Generation from Alkali Borohydride Solution (알칼리 붕소 수소화물 용액에서 수소발생을 위한 촉매에 관한 연구)

  • 정성욱;조은애;오인환;김성현;남석우
    • Proceedings of the Korea Society for Energy Engineering kosee Conference
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    • 2004.05a
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    • pp.119-124
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    • 2004
  • 최근 소형 다기능, 고기능 전자장비의 개발로 인하여 고밀도 전원이 필요성이 대두되고 있다. 연료전지는 높은 전력 밀도를 가짐으로 인하여 소형 전원 장비에 가능성을 가지는 것으로 여겨지고 있다. 연료전지 중 특히 고분자 전해질 연료전지는 높은 전력밀도와 낮은 작동온도 등으로 인하여 이차전지의 자리를 대체할 수 있을 것으로 보여지지만 현재까지 연료 공급장치의 문제로 인하여 실용화되지 못하고 있다. 본 연구에서는 여러 가지 수소저장 물질 중에서 알칼리 붕소 수소화물을 이용하여 연료전지에 수소를 공급하고자 하며, 수소발생에 사용되는 촉매에 대한 연구를 진행하였다. 알칼리 붕소 수소화물의 수소발생 반응에 사용되는 촉매로는 Pt, Ru, Co, Ni 등이 사용되어질 수 있다. 이중에서 가장 수소발생 능력이 높은 촉매는 Ru이며, 비귀금속 촉매 중에는 Co가 높은 활성을 나타내었다. 본 연구에서는 Ru 촉매와 Co 촉매의 특성을 비교하였으며 연료전지에 수소를 공급할 수 있는 가능성을 확인하였다. Ru와 Co 촉매의 공통적인 특징은 알칼리 붕소화물인 NaBH$_4$의 농도가 높아질수록, 온도가 높아질수록 수소의 발생속도를 높이는 현상을 보였다. 또한 안정화제인 NaOH에 대하여, Ru의 경우는 농도가 높아질수록 수소발생 속도가 낮아졌으며, Co는 그 반대의 결과를 보였다. NaBH$_4$의 분해 반응으로 발생된 수소를 연료전지에 공급하여 2W급의 휴대폰용 연료전지를 구동할 수 있었다. 이로써 알칼리 붕소수소화물로부터 발생된 수소를 이용하여 안정적으로 연료전지를 구동할 수 있는 가능성을 확인하였다. 유류 분해정도를 파악하는 지시자로써 특정 무기 오염물질을 이용할 수 있을 가능성이 있으므로 좀더 이들 관계성에 대한 연구가 진행될 필요성이 있다고 판단된다.고 과학적으로 분석할 수 있는 방법이 될 수 있을 것으로 기대된다. 의미를 되새기는 것으로 짧은 연구를 시작하겠다. 등은 활성 값이 70% 이상으로 퇴적물 독성이 상대적으로 낮았다. 이중나선 DNA 함량은 28.4 % - 49%로 대조군에 비해서 감소가 크다. 대부분의 정점이 대조군의 30% 내외로 정점 간의 차이는 크지는 않다. 그러나 다른 측정자료와 같이 정점 22에서 18%로 최소치를 나타내고, 정점 2, 12에서 20% 내외의 값을 보인다. 종합적으로 볼 때 오염물질의 유입이 크고, 광양제철 인근 정점 들이 모두 다른 정점에 비해서 낮아서, 퇴적물 독성이 높은 정점으로 조사되었다.hiwo의 광합성 능력은 낮은 농도들에서는 대조구와 유사하였으나, 5 $\mu\textrm{g}$/l의 높은 농도에서는 초기에 매우 낮은 광합성 능력을 보이다가 시간이 경과하면서 대조군보다 더 높은 경향을 나타냈다. 이러한 결과는 식물플랑크톤이 benso[a]pyrene의 낮은 농도에서 노출될 때는 이 물질을 탄소원으로 사용할 가능성이 있음을 시사한다. 본 연구의 결과들은 연안해역에 benso[a]pyrene과 같은 지속성 유기오염물질이 유입되었을 때 내정여부에 따라 식물플랑크톤 군집내 종 천이와 일차생산력에 크게 영향을 미칠 수 있음을 시사한다.TEX>5.2개)였으며, 등급별 회수율은 각각 GI(8.5%), GII(13.4%), GIII(43.9%), GIV(34.2%)로 나타

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Analytical Method of Silicon Dioxide in Health Functional Food Products using ICP-OES

  • Ka, Mi-Hyun;Lee, Kwang-Geun;Lim, Heung-Youl;Lee, Gunyoung;Yun, Sang Soon;Lim, Ho Soo;Kim, Yong-Suk
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.343-347
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    • 2017
  • The analytical method of silicon dioxide ($SiO_2$) in health functional food products was developed employing inductively coupled plasma optical emission spectrometry (ICP-OES) method assisted by acid (hydrofluoric acid and boric acid) digestion in open system without alkali fusion. The limit of detection (LOD) and limit of quantification (LOQ) of this method were found to be 0.07 and 0.20 mg/L, respectively. Linearity ($r^2$) and linear range were 0.99 and 0.20~20.0 mg/L, respectively. The accuracy and precision of $SiO_2$ (0.4, 1.0, and 2.0%, w/w) in spiked glucosamine exhibited to be the range of 90.22~94.14% and 0.72~1.67%, respectively. The contents of $SiO_2$ in 11 health functional food products were detected in range of 0.02~1.80% (w/w). Every sample showed below content of the permitted use level (2%, w/w) of $SiO_2$. Therefore ICP-OES method with acid can analyze the content of $SiO_2$ in health functional food products easily and rapidly. Consequently, the application of specification analysis of $SiO_2$ in health functional food products could be a significant work.

Effect of Green Tea(Camellia sinensins) and Maesil(Prunus mime) Extracts on Endogenous Formation of N-Nitrosodimethylamine (녹차 및 매실 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향)

  • 성낙주;최선영;정미자;신정혜;김행자
    • The Korean Journal of Food And Nutrition
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    • v.15 no.1
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    • pp.16-22
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    • 2002
  • The effects of nitrite scavenging. electron donating and N-nitrosodimethylamine(NDMA) formation in vitro arid green tea(Camellia sinensis) and Maesil(Prunus mume) were studied. The green tea and Maesil extracts were tested for their nitrite-scavenging effect under the different pH conditions such as pH 1.2, 4.2 aud 6.0. The effects of nitrite-scavenging in all concentrations were diminished in the alkali condition, while its effects in the acidic condition of pH 1.2 were reached of more than 99.0% by adding above 0.5ml of green tea extract. And also, nitrite-scavenging effect by adding 3ml of Maesil extract was about 77.0%. The electron donating ability(EDA) of green tea and Maesil extracts was 70.6%, 75.1%, respectively. The formation of NDMA was very effectiveness which was inhibited 82.1%, 73.2% at reaction mixture of pH 2.5 adding 3ml of above extracts. respectively . The ground CW. TW1 and TW2 (refer to Table 1) diets were incubated with 10m1 simulated saliva and 40m1 gastric juice at 37$^{\circ}C$ for 2hrs. NDMA formation was inhibited at all levels of green tea and Measil extracts.