• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.1
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• pp.53-61
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• 1985

Effects of nitrogen incorporated with 15N-nitrogen, which was applied to grown alone and in mixture with grass and legumes of forage crops, on the yield of dry matter, total nitrogen content, percentage of nitrogen derived from fertilizer (P.N.D.F.), A-value were studied, and the results obtained are as follows: 1. With nitrogen for the yield of dry matter was highly effective only to orchardgrass and were also effect to the early stages of alfalfa and birdsfoot trefoil in case of a mixed sowing. 2. Alone and mixed sowings showed the yield of dry matter in decreasing order of alfalfa<red clover>orchardgrass>birdsfoot trefoil, and orchardgrass + alfalfa>orchardgrass + red clover>orchardgrass + birdsfoot trefoil, respectively. 3. In case of grown alone, orchardgrass gave higher percentage of nitrogen derived from fertilizer (P.N.D.F.) than legume, whereas in case of grown in mixture it was in reverse order throughout the plots. It appears likely that orchardgrass was rather supplied soil nitrogen by legume then by nitrogen application. And P.N.D.F. was getting smaller in the latter stage of all forage crops in case of grown alone. In case of grown in mixture, however, and inconsistent P.N.D.F. was obtained from orchardgrass. 4. The A-value in case of grown alone was decreased in order of alfalfa>red clover>orchardgrass>birdsfoot trefoil. In contrast, however, the A-value in case of grown in mixture wes decreased in order of orchardgasss+alfalfa (3:7)>orchardgrass+red clover (3:7)>orchardgrass+red clover (3:7)>orchardgrass+alfalfa(5:5)>orchardgrass+birdsfoot trefoil (5:5)>orchardgrass+red clover (5:5)>orchardgrass+birdsfoot trefoil (3:7).

• Journal of Environmental Science International
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• v.30 no.2
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• pp.145-152
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• 2021

Soil conservation management is necessary for sustainable agriculture, in highland areas, and cover crops are one of the best soil conservation methods for slopes. In this study, we evaluated the effects of alfalfa cultivation on maize production, as well as soil conservation and quality. There was an outstanding soil conservation effect with alfalfa cultivation in the fallow and maize growing seasons. In particular, alfalfa cultivation reduced soil loss by up to 98% compared with bare field. It also increased the activities of soil microorganisms and the supply of organic matter. Maize production with alfalfa cultivation showed no significant differences in yield. In conclusion, alfalfa is an advantageous perennial cover crop in highland agricultural slope areas, which can have positive effects on soil quality and conservation, as well as maize production.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.177-182
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• 2023

Iron (Fe) is a vital element for plants and other organisms, involving in several physiological processes including respiration, chlorophyll biosynthesis, and photosynthesis. Unfortunately, how Fe accumulation regulates in response to light quality has not been well established in plants. Therefore, the aim of the study was to explore the mechanism of Fe homeostasis by light quality. In this study, we found morpho-physiological attributes were significantly improved in response to blue (λmax: 450) compared to white (λmax: 500) and red (λmax: 660) light. The root-shoot length, plant biomass, photosynthesis efficiency (Fv/Fm) and leafgreen (SPAD) significantly declined in response to white and red light. However, these parameters were improved and iron deficiency was substantially alleviated by blue light exposure in alfalfa seedlings. This study might be useful to the forage breeders and farmers for improving alfalfa yield and nutritional benefits.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.6
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• pp.800-809
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• 2011

The objective of this study was to investigate the effects of different ratios of corn silage (CS): alfalfa hay (AH), and extruded soybeans (ESB) on milk yield, milk composition, blood metabolites, and fatty acids in milk fat and plasma. Ninety multiparous Holstein cows were arranged in a randomized block design experiment which lasted 14 weeks. Treatments were arranged as a $3{\times}3$ factorial with 0%, 5% or 10% ESB (dry matter basis) and three forage treatments: I) 30% CS, 10% AH and 10% Leymus chinense hay (LC); ii) 20% corn silage, 20% alfalfa hay and 10% LC; iii) 10% CS, 30% AH and 10% LC. Cows were allowed to consume a total mixed ration ad libitum. There was no change of dry matter intake when cows were fed the experimental diets. As more AH was added to the diets, milk yield, milk protein content and yield, and trans9, cis11-conjugated linoleic acids (CLA) concentrations in milk fat and plasma increased. When ESB were supplemented to the diets, milk yield, and trans9, cis11-CLA concentration in milk fat and plasma increased. When 10% ESB was added to the diet containing 30% AH the trans9, cis11-CLA content (1.46 g/100 g of total fatty acids) in milk was the highest among all treatments. These results suggests that AH could replace part of a CS diet and be a good forage source of diet for dairy cows to improve milk yield and milk composition. Meanwhile, ESB could be included in the diet with high AH to improve production performance of dairy cows.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.1
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• pp.37-43
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• 1975

Effect of inoculation trial on plant height and dry matter yield of seven alfalfa varieties (Medicago sativa L. var. Washoe, Ranger, Lahontan, Narragensett, Atlantic, Vernal, and Moapa 69) which are considered to be well adapted in these areas were conducted during 1973-1974 in a relatively poor soil at the field station in Kyung Hee University. Root nodule bacteria: Rhizobium meliloti (Noculator Northrup Kings Co. Ltd) was applied at a rate to provide 6 oz/30l of alfalfa seeds. Seven varieties were seeded in pots on May 31, 1973 and in second year, they were transplanted in field. 1. Dry matter yield and plant height of all inoculation plots were significantly greater than those of the no inoculation plots in all varieties. 2. In the 1973, dry matter yield of inoculation plot a in pot experiment was in the order of Moapa 69> Vernal> Lahontan> Washoe> Atlantic> Narragensett> Ranger. 3. There was no difference in dry matter yield between with and without-inoculation after transplanted in a field in the second year (1974). Total dry matter yield of varieties was in the order of Washoe> Lahontan> Moapa 69> Ranger> Narragensett> Vernal> Atlantic.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.168-176
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• 2023

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.1
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• pp.50-55
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• 1983

Effects of nitrogen applied to grown alone and in mixture with grass and legumes of forage crops on the yield of dry matter, absorptivity, and the amount of nitrogen fixation were studied using $^{15}N$ ammonium sulfate. 1. The amount of nitrogen fixation in legume was decreased in order of alfalfa > red clover > birdsfoot trefoil, and red clover gave highest soil-nitrogen dependency. 2. Fertilizer nitrogen for the yield of dry matter was highly effective only in orchardgrass, and effective at the early stages of alfalfa and birdsfoot trefoil in case of a mixed sowing. 3. Alone and mixed sewings showed the yield of dry matter in decreasing order of alfalfa > red clover > orchardgrass > birdsfoot trefoil and orchardgrass + alfalfa > orchardgrass + red clover > orchardgrass + birdsfoot trefoil, respectively. 4. The plot of with nitrogen showed greater nitrogen uptake than the plot of without-nitrogen throughout the plots. In case of grown alone, however, difference in the absorptivity of various forage crops was decreased in order of alfalfa > red clover > orchardgrass > birdsfoot trefoil. 5. Regardless of nitrogen application, nitrogen uptake amount of orchardgrass was higher in the plot of mixture then in the plot grown alone, except the with nitrogen plot of red clover grown in mixture at the ratio of 3 : 7.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.3
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• pp.375-386
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• 2019

Objective: We hypothesized that silage additives may alter the undigested neutral detergent fiber (uNDF) content through ensiling. Therefore, urea and formic acid were applied to corn, whole barley crop (WBC) and alfalfa to change uNDF content of the ensiled forages. Methods: Six experimental diets at two groups of high uNDF (untreated corn and alfalfa silages [CSAS] and untreated whole barley and alfalfa silages [BSAS]) and low uNDF (urea-treated corn silage+untreated alfalfa silage [$CS_UAS$], urea-treated whole barley silage+untreated alfalfa silage [$BS_UAS$], untreated corn silage+formic acid-treated alfalfa silage [$CSAS_F$], and untreated whole barley silage+formic acid-treated alfalfa silage [$BSAS_F$]), were allocated to thirty-six multiparous lactating Holstein dairy cows. Results: The untreated silages were higher in uNDF than additive treated silages, but the uNDF concentrations among silages were variable (corn silage0.05). Milk yield tended to increase in the cows fed high uNDF diets than those fed low uNDF (p = 0.10). The cows fed diet based on urea-treated corn silage had higher milk yield than those fed other silages (p = 0.05). The substitution of corn silage with the WBC silage tended to decrease milk production (p = 0.07). Changing the physical source of NDF supply and the uNDF content from the corn silage to the WBC silage caused a significant increase in ruminal $NH_3-N$ concentration, milk urea-N and fat yield (p<0.05). The cows fed diets based on WBC silage experienced greater rumination time than the cows fed corn silage (p<0.05). Conclusion: Administering additives to silages to reduce uNDF may improve the performance of Holstein dairy cows.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.7
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• pp.960-964
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• 2014

An experiment was conducted to determine the effects of diets containing coarse-texture rice straw and small particle size alfalfa pellets as a part of total mixed ration (TMR) on milk productivity and chewing activity in lactating dairy cows. Sixteen multiparous Holstein dairy cows ($670{\pm}21kg$ body weight) in mid-lactation ($194.1{\pm}13.6$ days in milk) were randomly assigned to TMR containing 50% of timothy hay (TH) or TMR containing 20% of rice straw and 30% of alfalfa pellet mixture (RSAP). Geometric mean lengths of TH and RSAP were found to be 5.8 and 3.6, respectively. Dry matter intake, milk yield and milk composition were measured. Moreover, eating and ruminating times were recorded continuously using infrared digital camcorders. Milk yield and milk composition were not detected to have significant differences between TH and RSAP. Dry matter intake (DMI) did not significantly differ for cows fed with TH or RSAP. Although particle size of TH was larger than RSAP, eating, ruminating and total chewing time (min/d or min/kg of DMI) on TH and RSAP were similar. Taken together, our results suggest that using a proper amount of coarse-texture rice straw with high value nutritive alfalfa pellets may stimulate chewing activity in dairy cows without decreasing milk yield and composition even though the quantity of rice straw was 40% of TH.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.2
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• pp.98-107
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• 1983

This experiment was carried out to determine the optimum fertilizer level of N-P-K for maximum yield of grasses at hill-land pasture. The pasture species tested were orchardgrass, tall fescue, alfalfa and ladino clover. The results are summarized as follows: 1. The difference in yield of treatment 1, 2, and 4, 5 was significant at the 5% level. In other word, alfalfa yields from 6-30-30 kg/10a and 6-40-30 kg/10a (N-P-K) application were significantly increased over the yields from 4-20-20 kg/10a and 4-30-20 kg/10a (N-P-K). In observing the yield from the treatment 5 where 10 kilograms of phosphate was additionally applied over the treatment 4, it was increased in yield but failed to show statistical significance. With these results, the most optimum rates of N-P-K for alfalfa production are 6-30-30 kg/10a. 2. Ladino clover showed the highest yield at treatment 6 with statistic significance at the 1 percent level over the other treatments. Therefore, NPK rates of 6-30-40 kg/10a would be the most optimal rates for maximum production of ladino clover. 3. Orchard grass yield showed significance at the 1 percent level among other treatments except treatment 3 and 4. The highest yield was also shown at treatment 6 where NPK rates applied 40-30-40 kg/10a. 4. Tall fescue yield showed high significance at the 1 percent except treatments between 2 and 3, 5 and 6. The highest yield was observed at treatment 6 where NPK was applied 40-30-40 kilograms per 10a. 5. Nitrogen content of dry matter was 0.91-1.05% in 20 kg of N and 1.05-1.26% in 30-40 kg of N per 10a. The absorption rate of nitrogen applied was 23-31% regardless of the nitrogen level. Potash content of dry matter was increased in accordance with increasing K rates. 6. The gross income was the highest on the N-P-K application of 4-20-30 kg/10a in alfalfa and ladion clover, 40-30-40 kg/10a in orchardgrass and 30-20-20 kg/10a in tall fescus.