• Journal of Embryo Transfer
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• v.31 no.3
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• pp.191-197
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• 2016

Historically, Korea old cattle had been consisted with various lines of coat color brindle, black and white-brown breeds or more. The two rare lines of black and white coat color are maintained for animal resources and preserved critically. The present study was carried out to evaluate potential usage of cysteamine supplementation during in vitro matration (IVM) and in vitro culture/production of embryo (IVP) by transvaginal ultrasound-guided follicle aspiration (Ovum Pick-Up: OPU) for the establishment of cryo-banking system. Immature slaughterhouse-derived cumulus-oocyte complexes (SL-COCs) were matured in IVM medium supplemented with 0, 0.1, 0.3 or 0.9 mM cysteamine, and then cultured in mSOF-BAS for 8 days after in vitro fertilization. The treatment of 0.1 mM cysteamine on SL-COCs showed higher rate of blastocyst, so OPU-derived COCs from rare breeds were matured in TCM media supplemented with or without 0.1 mM cysteamine, FSH and 5% FBS. The embryos were evaluated their developmental stages on day 8. During IVM, cysteamine treatment significantly increased the embryo production rate of slaughterhouse-derived COCs (19.6% vs. 30.5%). The presence of cysteamine during IVM of OPU-derived COCs from rare Korean cattle breeds (albino white and black line) also increased embryo production rates than those from SL-COCs (27.4% vs. 41.9% and 36.4%). With these results, cysteamine treatment during IVM is one of key factors IVP of blastocysts to establish banking system of endangered rare Koarean cattle with OPU derived transferable blastocysts.

• Journal of Nutrition and Health
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• v.7 no.4
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• pp.1-5
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• 1974

Forty weaning albino rats were used to find the effects of alternate feeding of high protein diet with different intervals on growth, feed efficiency, protein efficiency ratio, weight of some internal organ, protein digestibility and nitrogen balance. Results obtained are summarized as follows: 1. The growth rate and feed efficiency of group B fed high protein and low protein alternately in every other day was equal to that of A group fed medium protein diet daily. However, the rats fed high protein diet once every three days or every four days could not result in same growth that obtained by group A. 2. The protein efficiency ratio of each group was the same without significant difference. 3. The liver weight appeared to be in proportion to final body weight, but intestine weight and length appeared not to be affected by treatments. 4. The protein digestibility in each group was insignificant. However, urinary nitrogen loss of intermittent high protein feeding group was lower than that of control group and the nitrogen retention ratio decreased with increased nitrogen intake.

• Korean Journal of Environmental Agriculture
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• v.7 no.1
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• pp.58-64
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• 1988

The acute oral, intraperitoneal and subacute toxicity of monocrotophos, an insecticide, was studied in albino rats. The acute oral $LD_{50}$ values for female and male rats were 5.0mg/kg and 8.7mg/kg, respectively. Conversely the intraperitoneal $LD_{50}$ values for female and male rats were 4.9mg/kg and 6.0mg/kg, respectively. Plasma cholinesterase (ChE) activity in rats treated with a single dose was the most depressed 4 hours after administration, but returned to normal within 72 hours of administration. Significant depression of body weight gain and food consumption was observed in rats receiving the 3.5mg/kg/day dose level for 28 days as compared with the controls. Additionally plasma ChE activity depression was also observed.

• The Korean Journal of Zoology
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• v.22 no.1
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• pp.1-10
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• 1979

From the experimental results of cellulose acetate membrane electrophoresis we concluded the followings in explaining the LDH isozyme patterns found in the retina and cerebrum of vertebrata. Lactate dehydrogenase of the retina and cerebrum of both Carassinus carassinus and Cyprinus carpio was found to have one diffused band located between $LDH_2$ and $LDH_1$. LDH isozyme patterns of heart, pectoral muscle, liver and stomach of the Cyprinus carpio had the same diffused band in all organs. LDH isozyme patterns of the cerebrum of Hynobius leechii and Rana nigromaculata were observed to be different, in Hynobius leeichi a single band moved to the negative pole and two bands of $LDH_5$ and $LDH_4$ were obtained in the Rana nigromaculata. The retina and cerebrum of Natrix tigrina lateralis were observed as one band but amyda maakii had different LDH isozymes of the retina and cerebrum. The retina of Amyda maakii had five distinct LDH isozyme bands which had decreasing activity in the order of $LDH_5, LDH_4, LDH_3, LDH_2 and LDH_1$. The cerebrum of Amyda maakii had one band like Natrix tigrina lateralis but it moved to the negative pole. LDH isozymes in the retina and cerebrum of Gallus gallus domesticus and Melopsittacus undulatus showed one band. Five characteristic LDH isozyme bands were obtained from the retina of mammals, Oryctolagus cuniclus, Canis familiaris, Sus scrofa bos taurus and in the cerebrum of mouse, albino rat, Rhinolophus ferrum-equinum kokai.

• Journal of the Korean Society of Food Science and Nutrition
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• v.6 no.1
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• pp.41-47
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• 1977

This study was designed to evaluate the nutritive value of pearl barley. Forty males and the same number of females of albino rats, the Leuise strain, weighing from 52.1g to 59.1g were divided into eight experimental groups, five males and females were composed of one group. A group: casein 15%+starch 75%+oil 8%+vitamin mixture 1%+salt mixture 1% B group: pearl barley 98%+vitamin mixture 1%+salt mixture 1% C group: rice 98%+vitamin mixture 1%+salt mixture 1% D group: barley 98%+vitamin mixture 1%+salt mixture 1% E group: rice 49%+pearl barley 49%+vitamin mixture 1%+salt mixture 1% F group: rice 73.5%+pearl barley 24.5%+vitamin mixture 1% +salt mixture 1% G group: barley 49%+pearl barley 49%+vitamin mixture 1% +salt mixture 1% H group: barley 73.5%+Pearl barley 24.5%+vitamin mixture 1%+salt mixture 1% The rats were kept in an individual cage and were fed 8 different diets for 7 weeks and fed by ad-libitum feeding method. The results of this study were eluciated as followings. 1. B group was significantly lower in weight gain than the other groups. 2. Polished pearl barley showed higher digestion rate in protein and carbohydrate than brown pearl barley but lower in fat. 3. Liver weight was obserbed significant differences between B group and C group. but no significant differences between the others. 4. B group was significantly lower in the total protein content of liver and A/G ratio in plasma than other groups. 5. B group was also the lowest in the hematocrit and hemoglobin contents among the experimental groups. 6. Free amino acid composition in plasma was similar to those in diet.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.5
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• pp.365-369
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• 2014

Cedrus deodara (Pinaceae) has been used traditionally in Ayurveda for the treatment of central nervous system disorders. 3,4-bis(3,4-dimethoxyphenyl)furan-2,5-dione (BDFD) was isolated from heart wood of Cedrus deodara and was shown to have antiepileptic and anxiolytic activity. Thus, the present study was aimed to explore its anti-depressant effect and to correlate the effect with serotonin and nor adrenaline levels of brain. Albino mice were used as experimental animal. Animals were divided in to three groups; vehicle control, imipramine (30 mg/kg i.p.), BDFD (100 mg/kg i.p.). Tail suspension test (TST) and forced swim test (FST) was performed to evaluate antidepressant effect of BDFD. BDFD (100 mg/kg, i.p.) showed a significant decrease in immobility time when subjected to FST whereas immobility time was not significantly altered in TST. BDFD treatment increased serotonin and noradrenaline levels in the brain which is indicative of BDFD having possible atypical antidepressant action.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.115-118
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• 1988

Mating experiments were performed to, elucidate the distribution of perfect state of Microsporum gypseum complex originated from animals and soils in Korea. A total of 30 strains of M. gypseum complex, composed of 16 from animals and 14 from soils, ere mated with the tester strains of Nannizzia incurvata, N. gypsea and N. fulva. Among 30 strains of M. gypseum complex examined, 16 strains(53.3%) were N. incurvata, 13 strains(43.3%) N. gypsea and 1 strain (3.3%) N. fulva. Among 13 strains of dog isolates, 8 strains(61.5%) were N. incurvata and 5 strains(38.5%) N. gypsea. And each strain isolated from Korean native goat, monkey and albino rat was N. gypsea. Among 14 strains of soil isolates, 8 strains(57.2%) were N. incurvata, 5 strains (35.7%) N. gypsea and 1 strain(7.2%) N. fulva. And -mating type was more frequently observed than +mating type in the strains of N. incurvata and N. gypsea. By this study, N. fulva was isolated the first time in Korea.

• Advances in Traditional Medicine
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• v.3 no.3
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• pp.133-140
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• 2003

Petroleum ether, chloroform and ethanol extracts of the seeds of Melia azedarach Linn. administered orally to male rats at the dose level of 25 mg/100 g body weight for 48 days showed antispermatogenic activity, as the number of spermatocytes, spermatids and spermatozoa was decreased. The total cholesterol content was increased while protein and glycogen contents were decreased. The acid phosphatase content was also decreased while that of alkaline phosphatase increased. At the same time the weight of caput and cauda epididymis, prostate gland, seminal vesicle and Levator Ani muscle was decreased indicating its antiandrogenic property. Of the three extracts, the petroleum ether extract was more potent in its antispermatogenic and antiandrogenic activities and did not produce any sings of toxicity upto a dose of 25 mg/100 g body weight oral administration. After subjecting it to preliminary phytochemical screening the petroleum ether extract showed positive tests for steroids and saponins.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.4
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• pp.310-315
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• 1983

The effects of DFP(Dietary Fiber Preparation) extracted from naked barley on the lipid metabolism of rats were investigated. 1. The composition of DFP was determined as cellulose 19.3%, hemicellulose 58.7%, lignin 10.5 % on dry weight basis. 2. DFP had no significant effect on the growth of rats. (p>0.05) 3. DFP had no significant effects on the composition of serum lipid and the lipid content per g of liver in rats, but caused significant decrease in serum and liver cholesterol levels of rats to a certain degree. (p<0.05)

• Applied Microscopy
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• v.24 no.1
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• pp.77-85
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• 1994

In order to investigating the pulmonary toxicity of the O-chlorobenzyledene malononitrile (CS), lacrimating agent, $2.6g/m^3$ of CS was inhalated to Sprague-Dawley rats in the plastic chamber for 20 minutes. The ultrastructural changes of type II pneumocytes in the lung were observed with Hitachi 600 transmission electron microscope. The results obtained were as follows: 1. 3 hours after exposure to CS the fusion of surface microvilli, dilatation of cristernae of the rough endoplasmic reticulum, atrophy of Golgi complex and condensation, deletion of lamellated membranes in lamellar bodies were observed in type II pneumocytes. 2. One and 2 days after CS-exposure, disorganization of mitochondrial double membranes, fragmentations of rough endoplasmic reticulum were found in the great alveolar cells. In addition, decrease in amount of polyribosome granules and deletion or condensation of lamellated membranes in lamellar bodies were also observed. 3. 4 days after exposure to CS, the type II pneumocyte revealed new whorled lamellar membranes in lamellar bodies, a few intact rough endoplasmic reticulum and restoration of polyribosome granules. It is consequently suggested that CS induces degenerative changes of cytoplasmic organelles in the type II pneumocytes.