• Journal of radiological science and technology
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• v.8 no.2
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• pp.47-63
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• 1985

This paper was aimed to study the effects of Cobalt-60 gamma ray irradiation on Albino rat blood cells and the activity of enzymes were measured using blood cell auto analyzer (Cell-Dyn 900) and enzyme autoanalyzer (Gilford 3500) respectively. Cobalt-60 gamma rays those are grouped into 200R, 400R, 600R, 800R, 1,000R, 1,200R, 1,300R, 1,400R, 1,500R and 1,600R to the rats of male and female and measured the numeral varieties of blood corpuscles of the rats and the active varieties of enzyme of acid, alkaline phosphatase (ACP, ALP), lactic acid dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase. (GPT) those came out after 1, 3, 5 or 7 days. The results are summarized as follows: 1. In both case of male and female, the numeral varieties of the erythrocytes, in the 200R group of irradiation showed the minimum value in the 3 days group and a tendency of increase in the 5 and 7 days groups, while the numeral varieties of the leucocytes in every groups of irradiation showed a tendency of a rapid decrease after a day. The numeral varieties of the blood platelet in both case of male and female didn't show generally any great change in a day and 3 days groups, but showed a rapid decreasing appearance after the 5 days. 2. In both case of male and female, the activities of ACP were on the decrease gradually after 3 days of the irradiation and the activities of ALP were on the decrease after 5 days. Similarly in both case of mate and female, the activities of LDH showed the decrease after 3 and 5 days, and the phenomenous of GOT showed an appearance of increase in a day, but decreased after 3 days. As for the GPT activities couldn't find any great change in the male rats in comparison with the control groups, but in the female rats a tendency of the increase in 600R, 800R, 1,000R groups after 3 days. 3. In the case of the irradiation of high quantity of ray more than 1,200R, all rats died after 4 days, and by the irradiation of 1,600R all rats died after 3 days, and it showed the sensitive response of a living body to the irradiation of high quantity.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.5
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• pp.417-422
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• 2013

The aim of this study was to evaluate the synergistic potentiation effect of ineffective doses of dexmedetomidine on antinociception induced by morphine and fentanyl in acute pain model in rats. Seventy albino Wistar rats were separated into 7 groups. Data for the control and sham groups were recorded. The ineffective dose of dexmedetomidine was investigated and found to be 3 ${\mu}g/kg$. Each group was administered the following medications: 3 mg/kg morphine (intraperitoneal) to Group 3, 5 ${\mu}g/kg$ fentanyl (intraperitoneal) to Group 4, dexmedetomidine 3 ${\mu}g/kg$ (subcutaneously) to Group 5, dexmedetomidine 3 ${\mu}g/kg$ (subcutaneous)+3 mg/kg morphine (intraperitoneal) to Group 6 and finally 3 ${\mu}g/kg$ dexmedetomidine (subcutaneous)+5 ${\mu}g/kg$ fentanyl (intraperitoneal) to Group 7. Just before the application and 15, 30, 60, 90 and 120 min after the administration of medication, two measurements of tail flick (TF) and hot plate (HP) tests were performed. The averages of the measurements were recorded. TF and HP latencies were the main outcomes. The analgesic effect of the combinations with dexmedetomidine+morphine (Group 6) and dexmedetomidine+fentanyl (Group 7), compared to the analgesic effect of morphine alone and fentanyl alone was significantly higher at 15, 30, 60 and 90 minutes after administration. In this study, dexmedetomidine in ineffective doses, when combined with morphine and fentanyl, potentiates the effects of both morphine and fentanyl.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8271-8279
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• 2016

Background: Hepato-carcinogenesis is multifaceted in its molecular aspects. Among the interplaying agents are altered gap junctions, the proteasome/autophagy system, and mitochondria. The present experimental study was designed to outline the roles of these players and to investigate the tumor suppressive effects of curcumin with or without mesenchymal stem cells (MSCs) in hepatocellular carcinoma (HCC). Materials and Methods: Adult female albino rats were divided into normal controls and animals with HCC induced by diethyl-nitrosamine (DENA) and $CCl_4$. Additional groups treated after HCC induction were: Cur/HCC which received curcumin; MSCs/HCC which received MSCs; and Cur+MSCs/HCC which received both curcumin and MSCs. For all groups there were histopathological examination and assessment of gene expression of connexin43 (Cx43), ubiquitin ligase-E3 (UCP-3), the autophagy marker LC3 and coenzyme-Q10 (Mito.Q10) mRNA by real time, reverse transcription-polymerase chain reaction, along with measurement of LC3II/LC3I ratio for estimation of autophagosome formation in the rat liver tissue. In addition, the serum levels of ALT, AST and alpha fetoprotein (AFP), together with the proinflammatory cytokines $TNF{\alpha}$ and IL-6, were determined in all groups. Results: Histopathological examination of liver tissue from animals which received DENA-$CCl_4$ only revealed the presence of anaplastic carcinoma cells and macro-regenerative nodules. Administration of curcumin, MSCs; each alone or combined into rats after induction of HCC improved the histopathological picture. This was accompanied by significant reduction in ${\alpha}$-fetoprotein together with proinflammatory cytokines and significant decrease of various liver enzymes, in addition to upregulation of Cx43, UCP-3, LC3 and Mito.Q10 mRNA. Conclusions: Improvement of Cx43 expression, nonapoptotic cell death and mitochondrial function can repress tumor growth in HCC. Administration of curcumin and/or MSCs have tumor suppressive effects as they can target these mechanisms. However, further research is still needed to verify their effectiveness.

• Korean Journal of Environment and Ecology
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• v.29 no.3
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• pp.401-409
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• 2015

The fish community and introduced fish were surveyed at eight stations of Lake Soyang from March to October, 2013. A total of 41 species of twelve families were collected from the survey stations and among them, two species: Hemibarbus mylodon and Siniperca scherzeri (albino type) were identified as natural monument. There were fifteen Korean endemic species (36.6 %) including Acheilognathus yamatsutae. When compared with previous data (2003), A. yamatsutae, Pseudorasbora parva, Sarcocheilichthys nigripinnis morii, Sarcocheilichthys variegatus wakiyae, Orthrias nudus, Leiocassisuss ussuriensis, Oncorhynchus mykiss, and Micropterus salmoides appeared new in the lake. Dominant species were Hypomesus olidus (St. 1, 3, 6, 7, 8), Lepomis macrochirus (St. 2, 4) and Zacco platypus (St. 5). Of the 12 introduced species fishes in Lake Soyang, Anguilla japonica, Hemiculter eigenmanni, H. nipponensis, Rhinogobius giurinus and Tridentiger brevispinis were introduced and released from other native river water zones in Korea but Cyprinus carpio (Israeli type), Carassius cuvieri, O. mykiss, Ictalurus puntatus, L. macrochirus, and M. salmoides originated from foreign countries.

• Archives of Plastic Surgery
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• v.49 no.3
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• pp.462-470
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• 2022

Background Reactive oxygen species cause serious damage to the physiological function of tissues. Determination of total antioxidant capacity of skin tissue is one of the determinants of damaged tissue function. Mast cells (MCs) are one of the groups of cells that are invited to the site of injury. The healing process begins with the rapid release of various types of MCs' intermediate factors at the site of injury. Bone marrow mesenchymal stem cell (BMMSC) production and secretion have been shown to regenerate the skin. The aim of this research was to evaluate the wound-healing and antioxidant effects of BMMSCs per MCs. Methods Fifty-four albino Wistar male rats were divided into three groups: (1) nonsurgery, (2) surgery, and (3) surgery + BMMSCs. Groups 2 and 3 were operated with a 3 × 8 cm flap and in group 3, cell injections (7 × 10⁹ cell injection at the time of surgery) were performed. After days 4, 7, and 15, percentage of the surviving tissue, histological characteristics, superoxide dismutase (SOD) activity, and amount of malondialdehyde (MDA) were measured in the groups. For results, Graph Pad Prism 8 software was used, and data were analyzed and compared by analysis of variance and Tukey test. Results BMMSCs' application decreased the amount of MDA, increased SOD activity and survival rate of the flaps, and improved the histological characteristics. Conclusion This study revealed the protective effects BMMSCs alongside MCs against oxidative stress on the survival of the flaps. However, for clinical use, more research is needed to determine its benefits.

• Journal of Ginseng Research
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• v.4 no.2
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• pp.146-164
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• 1980

In this paper it was attempted to observe the effect of defatted panax ginseng supplement of the growth rate, feed and protein efficiency ratios, and the contents of cholesterol, total lipid and protein in the serum, liver and aorta in Sprague-Dowley Albino male rat (weighing 83 ${\pm}$ 4 g). Seven kinds of experimental diets were prepared as follows : Stock (control) diet, ginseng control diets supplemented with 0.5, 1.0 and 3.0% of ginseng powder to the stock diet, and defatted ginseng Powder diets supplemented with 0.5, 1.0 and 3.0% of defatted ginseng powder to the stock diet. .All diets contained same level of lipid and protein, respectively. The results obtained are as follows : 1. The growth rate in the feeding group of 0.5% defatted ginseng powder diet for 16week were higher than other diet groups. 2. Feed and Protein efficiency in 0.5% defatted ginseng group showed similar tendency to that in body growth rate. 3. The total cholesterol contents in the serum of 0.5% defatted ginseng Powder diet$.$ group showed the tendency to decrease gradually for 4 to 12 weeks, maintaining higher level than other groups. The free cholesterol contents in the serum of defatted ginseng powder diet group in 8 the and 16th weeks were higher than all ginseng control diet group. The total and free cholesterol contents in the liver of all defatted ginseng diet groups in 16th week were higher than hose of all ginseng control groups. The total cholesterol content at 12h week and the free cholesterol content at 16th week i n the aorta of all defatted ginseng diet groups were lower than those of ginseng control groups, respectively. 4. The total lipid contents in the serum of 1.0 and 3.0% defatted ginseng diet groups at 2nd to I2th weeks were lower than other groups, and those in the liver and aorta of all defatted ginseng diet groups at 12th weeks were lower than those of ginseng control diet groups. 5. The protein contents of the serum and aorta were continuously increased throughout whole experimental period in all experimental groups. The protein content of the liver of all groups were decreased at 2nd week and after then no change was observed.

• Journal of Nutrition and Health
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• v.8 no.3
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• pp.19-29
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• 1975

This study was designed to compare the metabolic effects of varicous types of Korean diet. 40 males and same number of females of Albino rats, divided into eight groups, 5 rats each. 1 Group; Sugar casein standard Group 2 Group; Rice casein standard Group 3 Group; Rice group 4 Group; 65% Rice +35% Anchovy 5 Group; 65% Rice +35% Chinese cabbage 6 Group; 65% Rice+10% Anchovy +25% Chinese cabbage 7 Group; 65%. Rice+26% Chinese cabbage+3.5% Anchovy+4% Bean+1% Potatoes+0.2% Sea Weeds+0.3% Seasonings 8 Group; 47% Rice+24% Chinese cabbage+20.5% Anchovy+5% Bean+1% Potatoes+1.8% Sea Weeds+0.7% Seasonings The rats were kept in individual cage and given 8 different diet for 10 weeks. The result of this study were elucidated as fallow. Food intake of sugar casein standard group and rice casein standard group and Seoul diet pattern group were high, Rice diet group showed low food intake. F.E.R, P.E.R, body weight, organ weight were the similar results. The nitrogen content in various organs were no great difference, but nitrogen metabolism and total nitrogen retention were significant differences. The lipid content in the liver showed no significant differences, but fecal lipid and serum cholesterol showed significant differences. This study showed the glucose content in urine and feces were due to the dietary carbohydrate content. In other word, the results of this study showed no significant differences between sugar casein standard group and rice casein standard group, but significiant differences between standard group and experimental group.

• Parasites, Hosts and Diseases
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• v.31 no.2
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• pp.83-90
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• 1993

We performed bromodeoxyuridine (BrdU) staining to observe the proliferation pattern of epithelial cells on the biliaJy mucosa in Clonorchis sinensis infection. Albino rats were infected with 100 metacercariae each and their livers were processed for histopathological observation after BrdU injection. Five to six sites in the liver of a rat were selected for paraffin section, and stained immunohistochemically to visualize BrdU incorporating cells. The flukes were mainly in the common bile duct and right or left hepatic bile ducts. The proportion of stained epithelial cells in the infected bile ducts where the worms were found on the section was 2.9-10.2% at 1 week after infection. 7.3-12.8% at 2 weeks, 7.3-13.4% at 5 weeks, and 8.4-14.8% at 15 weeks while in the non-infected ducts o to 2.7% cells were stained. The stained cells were mainly at the base of the mucosal layer. It is suggested that mucosal epithelial cells of the bile ducts infected with C. sinensis become hyperplastic mainly by direct and local stimulation of the worms.

• The Journal of Korean Academy of Prosthodontics
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• v.15 no.1
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• pp.26-33
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• 1977

Present study investigated the effect of Nuva-Lite irradiation on the labial oral mucosa of the albino rats. The specimens were embedded in paraffin wax and stained with histological and histochemical procedures such as hematoxylin-eosin stain, Feulgen reaction, methyl green-pyronin stain, alloxan Schiff reaction, -SH group reaction, PAS reaction, colloidal iron reaction and toluidine blue stain. The specimens 24 hours to 3 days after ultraviolet irradiation exhibited mild intracellular edema in the prickle cell layer. On 6th and 10th day after irradiation rete peg hyperplasia was prominent. Ultraviolet irradiation caused diminution of Feulgen reacted DNA. However, sulfhydril and ${\alpha}$-amino acid radicals were increased at the upper layer of stratified squamous epithelium after irradiation.

• Korean Journal of Ichthyology
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• v.11 no.2
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• pp.163-171
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• 1999

The complete sequences of mtDNA control regions of six salmonines were determined: 1089 bp in lenok (Brachymystax lenok); 999 bp in cherry salmon (Oncorhynchus masou masou) and Ishikawa's cherry salmon (O. masou ishikauiae); 1002 bp in chum salmon (O. keta), and 1003 bp in rainbow trout (O. mykiss) and an albino mutant of rainbow trout. The estimated interspecific sequence divergences from PCR/direct sequencing data ranged from 5.42% to 16.49%. The organization of this region is similar to that of other vertebrates. A 81 bp tandemly repeated sequence, associated with length variation was observed in the 3' end of the salmonids control region in this study. In addition, The phylogenetic tree based on the control region sequences supported that cherry salmon was closer to chum salmon than to rainbow trout, while lenok was most distantly related species among six salmonines.