• Korean Journal of Veterinary Research
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• v.57 no.2
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• pp.113-116
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• 2017

The aim of this study was to examine normal bronchi in dogs by using virtual bronchoscopy (VB) and to evaluate the utility of VB in clinical practice. The bronchi of six dogs without tracheobronchial disease were visualized by VB. Airways from the tracheal bifurcation to the lobar bronchi were well visualized in all dogs. Segmental and subsegmental bronchi were also well identified, but the degree and number of those varied with dog size. The mean numbers of segmental and subsegmental bronchi identified in the six dogs were 41.83 and 50.17, respectively, whereas, the mean numbers in medium- and large-sized dogs were 55.00 and 82.67, respectively, and in small-sized dogs, the means were 28.67 and 17.67, respectively. Although there were size-dependent differences in VB visualization of the bronchi, it was possible to identify peripheral airways to the subsegmental bronchi level, which can rarely be accomplished via conventional bronchoscopy. VB is the noninvasive method that can be used to examine bronchial anatomy, and our results suggest that VB can be useful for evaluating bronchi, including segmental and subsegmental ones that cannot be examined routinely by conventional bronchoscopy. Thus, VB has potential as an alternative to conventional bronchoscopy in the examination of bronchi in dogs.

• Tuberculosis and Respiratory Diseases
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• v.41 no.2
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• pp.73-86
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• 1994

In addition to classic cholinergic and adrenergic pathways, the existence of a third division of autonomic control in the human airways has been proved. It is called a nonadrenergic noncholinergic(NANC) nervous system, and difficult to study in the absence of specific blockers. Neuropeptides are certainly suggested to be transmitters of this NANC nervous system. It is very frustrating to understand the pathophysiologic role of these peptides in the absence of any specific antagonists. However, further studies of neuropeptides might eventually lead to novel forms of treatment for bronchial asthma. Another study of the interaction between different components of the autonomic nervous system, either in ganglionic neurotransmission or by presynaptic modulation of neurotransmitters at the end-organ will elute neural control in airway disease, particularly in asthma. Studies of how autonomic control may be disordered in airway disease should lead to improvements in clinical management. Epithelial damage due to airway inflammation in asthma may induce bronchial hyperresponsiveness. Axon reflex mechanism is one of possible mechanisms in bronchial hyperresponsiveness. Epithelial damage may expose sensory nerve terminals and C-fiber nrve endings are stimulated by inflammatory mediators. Bi-directional communication between the nerves and mast cells may have important roles in allergic process. The psychological factors and conditioning of allergic reactions is suggested that mast cell activation might be partly regulated by the central nervous system via the peripheral nerves. Studies in animal models, in huamn airways in vitro and in patients with airway disease will uncover the interaction between allergic disease processes and psychologic factors or neural mechainsms.

• Tuberculosis and Respiratory Diseases
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• v.54 no.1
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• pp.80-90
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• 2003

Background : Goblet cell hyperplasia is a critical pathological feature in hypersecretory diseases of the airways. A bacterial infection of the lung is also known to induce inflammatory responses, which can lead to the overproduction of mucus. Recently, mucin synthesis in the airways has been reported to be regulated by neutrophilic inflammation-induced epidermal growth factor receptor (EGFR) expression and activation. In addition, it was reported that migration of the activated neutrophils is dependent on the matrix metalloproteinases (MMPs), especially MMP-9. In this study, bacterial lipopolysaccharide (LPS)-induced goblet cell hyperplasia and mucus hypersecretion by EGFR cascade, resulting from the MMPs-dependent neutrophilic inflammation were investigated in the rat airways. Methods : Pathogen-free Sprague-Dawley rats were studied in vivo. Various concentrations of LPS were instilled into the trachea in $300{\mu}{\ell}$ PBS (LPS group). Sterile PBS ($300{\mu}{\ell}$) was instilled into the trachea of the control animals (control group). The airways were examined on different days after instilling LPS. For an examination of the relationship between the LPS-induced goblet cell hyperplasia and MMPs, the animals were pretreated 3 days prior to the LPS instillation and daily thereafter with the matrix metalloproteinase inhibitor (MMPI; 20 mg/Kg/day of CMT-3; Collagenex Pharmaceuticals, USA). The neutrophilic infiltration was quantified as a number in five high power fields (HPF). The alcian blue/periodic acid-Schiff (AB/PAS) stain were performed for the mucus glycoconjugates and the immunohistochemical stains were performed for MUC5AC, EGFR and MMP-9. Their expressions were quantified by an image analysis program and were expressed by the percentage of the total bronchial epithelial area. Results : The instillation of LPS induced AB/PAS and MUC5AC staining in the airway epithelium in a time- and dose-dependent manner. Treatment with the MMPI prevented the LPS-induced goblet cell hyperplasia significantly. The instillation of LPS into the trachea induced also EGFR expression in the airway epithelium. The control airway epithelium contained few leukocytes, but the intratracheal instillation of LPS resulted in a neutrophilic recruitment. A pretreatment with MMPI prevented neutrophilic recruitment, EGFR expression, and goblet cell hyperplasia in the LPS-instilled airway epithelium. Conclusion : Matrix metalloproteinase is involved in LPS-induced mucus hypersecretion, resulting from a neutrophilic inflammation and EGFR cascade. These results suggest a potential therapeutic role of MMPI in the treatment of mucus hypersecretion that were associated with a bacterial infection of the airways.

• The Korean Journal of Nuclear Medicine
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• v.28 no.2
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• pp.192-199
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• 1994

Diffuse pahbronchiolitis(DPB) is a relatively new, chronic, nonspecific, inflammatory disease of the lung that typically involves the airways in the "transitional" zone of the lung. Clinically, DPB strongly resembles chronic obstructive pulmonary disease (COPD) and the distinction between the two is crucial because the former is often fatal, requiring different therapeutic strategies. This study was prospectively carried out to assess diagnostic usefulness of radioaerosol scan(RAS) in DPB. RAS findings were analyzed with respect to the location and extent of abnormal aerosol deposition in the lung divided into the central, intermediary, and transitional airways and the peripheral airspaces. RAS showed mottled aerosol deposits characteristically in the transitional and intermediary airways with peripheral airspace defects. Such a deposition pattern contrasted sharply with central aerosol deposition of COPD. In conclusion, RAS appears to be a convenient, noninvasive, and useful diagnostic method of DPB.

• Journal of KIISE:Software and Applications
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• v.33 no.11
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• pp.942-952
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• 2006

We propose an automatic segmentation method for identifying pulmonary structures using gray-level information of chest CT images. Our method consists of following five steps. First, to segment pulmonary structures based on the difference of gray-level value, we select the threshold using optimal thresholding. Second, we separate the thorax from the background air and then the lungs and airways from the thorax by applying the inverse operation of 2D region growing in chest CT images. To eliminate non-pulmonary structures which has similar intensities with the lungs, we use 3D connected component labeling. Third, we segment the trachea and left and right mainstem bronchi using 3D branch-based region growing in chest CT images. Fourth, we can obtain accurate lung boundaries by subtracting the result of third step from the result of second step. Finally, we select the threshold in accordance with histogram analysis and then segment radio-dense pulmonary vessels by applying gray-level thresholding to the result of the second step. To evaluate the accuracy of proposed method, we make a visual inspection of segmentation result of lungs, airways and pulmonary vessels. We compare the result of the conventional region growing with the result of proposed 3D branch-based region growing. Experimental results show that our proposed method extracts lung boundaries, airways, and pulmonary vessels automatically and accurately.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.14 no.2
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• pp.129-132
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• 2003

Chlorine gas is highly irritating gas that, when inhaled, can damage larger airways as well as distal lung structure. It occurs usually result in mild ocular, oropharyngeal, or respiratory symptom and recovery may proceed slow for several weeks. We reported four cases of dysphonia due to acute chlorine inhalation during a swimming pool accident were treated by voice therapy and medication.

• Journal of Chest Surgery
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• v.26 no.8
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• pp.664-666
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• 1993

A technique for insertion of a long silicone T-tube in patient with critical stenosis and high-risk resection and primary anastomosis of long segment of the distal trachea is presented. It was not easy to insert a long T-tube by existing methods because of flexibility of a T-tube and tightness of stenosis. So we used a silastic endotracheal tube and guiding wire as stylet of a T-tube. During insertion, ventilation was normally maintained through the lumen of endotracheal tube. This provided rapid relief from airway obstruction and asphyxation and is a easy, safe and effective method to restore patency of the major airways.

• Journal of Yeungnam Medical Science
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• v.16 no.1
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• pp.15-24
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• 1999

Although traditionally viewed as a physical barrier between the host and a variety of inhaled irritants and pathogens, it has become clear that the epithelium has a much broader functional scope. Epithelial cells arc metabolically active and can play an important role in the regulation of the allergic inflammatory response. This review provides a consideration of the role of the epithelial cell as both a "target" for exogenous and endogenous stimuli and as an "effector" cell that is capable of producing a variety of products that can influence the inflammatory response in the airways.

• 대한예방의학회:학술대회논문집
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• 1996.10a
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• pp.287-288
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• 1996

• Tuberculosis and Respiratory Diseases
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• v.44 no.6
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• pp.1218-1224
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• 1997

세기관지를 침범하는 많은 질환들이 세기관지에 유사한 병리소견을 일으킴으로 이들의 원인, 병인, 진단을 위하여서는 임상 및 병리소견을 연관시킴이 거의 필수적이다. Small airways disease를 포함한 세기관지 질환의 병리소견 특히 호흡성 세기관지염, bronchiolitis obliterans organizing pneumonia, constrictive bronchiolitis obliterans 등에 대하여 중점적으로 기술하였다. BOOP은 어떤 급성 폐손상에 대한 비특이성 소견이며 조직병리적인 기술이다. 반면 Idiopathic BOOP은 COP와 동의어로써 특정한 질환이며 이의 주된 병리소견이 BOOP인 것으로 이해하는 것이 좋겠다. Constrictive bronchiolitis obliterans는 임상적으로나 병리학적으로 BOOP과는 다르다고 하겠다.