• 제목/요약/키워드: Agriculture and Forestry

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일본(日本)의 임목육종(林木育種) 및 삼림경영연구동향(森林經營硏究動向) (The Tendency of Scientific Research of Tree Improvement and Forest Management in Japan)

  • 김영호;손두식
    • Current Research on Agriculture and Life Sciences
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    • 제2권
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    • pp.42-55
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    • 1984

  • The direction of scientific researches on tree improvement and forest management in several universities and research institutes in Japan can be summarized as follows: They put a great emphasis on sugi, Cryptomeria japonica and cypress, Chamaecyparus oblusa which are two major conifer species largerly planted in the Japanese forestry. In the research of sugi, a great concern has been made in evaluating inheritance of forest tree, quantitative characters and genetic parameter of growth, and in breeding for resistance to diseases and insects and to all the natural calamities. Interaction between environmental conditions and genetic nature of tree can be concerned factors in relation with forest damage, together with silvicultural conditions and pest infestation. Selfing hybrids of $F_1$ made from crossing twisted-leaf sugi, defomity leaf type and midori sugi, normal leaf type segregated the normal needle, twisted needle, green leaf and albino leaf type. It seemed that separation of many defomity individuals can be governed by two dominant complementary genes and from the near loci of which it was detected lethal genes. 52% of Japanese forestry is occupied by the small forest landowners like Korean forestry. This made difficulty for forest improvement such as progressive afforestation and for capital accumulation form forestry. The Forest Corporation was established at first in 1959 to aming at productive forestry structure and forest management, and afforestation. For these purpose, 35 Forest Corporations are at moment operating throughout Japan. However, investment in forestry business becomes less attractive since the wage in forest production duction increased in higher trend. than timber price. Therefore, an artifical afforestation becomes yearly decreased. At present. the self-sufficient rate of timber production in Japan is about 35%, and so a great effort is being made to increase self-sufficient rate of timber production.

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Expression of Codon Optimized β2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of β-Agonist Residues in Pork

  • Liu, Yuan;Wang, Jian;Liu, Yang;Yang, Liting;Zhu, Xuran;Wang, Wei;Zhang, Jiaxiao;Wei, Dong
    • Journal of Microbiology and Biotechnology
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    • 제29권9호
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    • pp.1470-1477
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    • 2019

  • ${\beta}_2$-adrenergic receptor (${\beta}_2-AR$) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of ${\beta}$-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and $59.57{\mu}g/l$, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was $3.2{\mu}g/l$ and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified ${\beta}_2-AR$ would have great application potential in detection of ${\beta}$-agonist residues.

  • https://doi.org/10.4014/jmb.1906.06022 인용 PDF KSCI