• 제목/요약/키워드: Agriculture Biotechnology

검색결과 2,957건 처리시간 0.03초

PQQ-Dependent Organic Acid Production and Effect on Common Bean Growth by Rhizobium tropici CIAT 899

  • Cho, Young-Shin;Park, Ro-Dong;Kim, Yong-Woong;Hwangbo, Hoon;Jung, Woo-Jin;Suh, Jang-Sun;Koo, Bon-Sung;Krishnan, Hari-B.;Kim, Kil-Yong
    • Journal of Microbiology and Biotechnology
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    • 제13권6호
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    • pp.955-959
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    • 2003

  • Rhizobium tropici CIAT 899 is capable of synthesizing inactive apo-glucose dehydrogenase (GDH). To become an active holo enzyme, the GDH requires a cofactor, PQQ. When R. tropici CIAT 899 was grown in a broth culture medium containing hydroxyapatite and pyrrolo quinoline quinone (PQQ), pH decreased while the concentration of soluble P increased. The solubilization of hydroxyapatite was associated with the production of gluconic acid and 2-ketogluconic acids. The organic acid production and P solubilization were greatly enhanced when the bacterium was grown with air supply. Effect of R. tropici CIAT 899 with (CI＋PQQ) and without PQQ (CI) on the common bean growth was examined. Shoot and root weight, and N and P contents in CI＋PQQ treatment, were significantly higher than those in control and CI treatment. Nodule weight and acetylene reducing activities were also significantly higher in CI＋PQQ treatment than in other treatments.

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Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture

  • Khaskheli, Allah Jurio;Ali, Muharam;Shah, Syad Zakir Hussain;Memon, Zohra Fatima;Awan, Saleem;Khaskheli, Muhammad Ibrahim;Khaskheli, Mohsin Ali;Magsi, Bilqees;Qambrani, Zareen;Khaskheli, Asad Ali
    • Journal of Plant Biotechnology
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    • 제48권2호
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    • pp.86-92
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    • 2021

  • The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).

  • https://doi.org/10.5010/JPB.2021.48.2.86 인용 PDF KSCI