• Title/Summary/Keyword: Agriculture Biotechnology

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In vitro and in vivo Transient Expression in Insect Cells Mediated by the Cationic Liposome DDAB/DOPE

  • Xiao, Qing-Li;Zhou, Ya-Jing;Zhang, Zhi-Fang;He, Jia-Lu
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.1
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    • pp.57-62
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    • 2002

  • Cationic liposomes complexed with DNA have been extensively utilized for the delivery of reporter or therapeutic genes both in culture and in vivo. We investigated and determined the optimum conditions of a cationic liposome, composed of dimethyldioctadecy-lammonium bromide (DDAB) and dioleoyl phosphati-dylethanolamine UOPE), mediated a reporter plasmid expressing luciferase into insect cell lines (Sf-21 and Bm-N) and silkworm larvae. Together the data demonstrated that Bombyx mori nuclear polyhedrosis virus (BmNPV) genomic DNA (128 kb) was successfully transfected into Bm-5 cells using this liposome. These results suggest that DDAB/DOPE liposome will be useful as delivery agents for gene transfer to insect cells both in vitro and in vivo.

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Transovarial Transmission of Bombyx mori Nuclear Polyhedrosis Virus in the Silkworm

  • Xiao, Qing-Li;Zhang, Zhi-Fang;Yi, Yong-Zhu;He, Jia-Lu
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.119-122
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    • 2001

  • Whether Bombyx mori nuclear polyhedrosis virus (BmNPV) can be transmitted to offspring, has been a noticeable question for a long time. When fifth instar larvae of the silkworm were orally inoculated with BmNPV dot hybridization and PCR amplification analysis demonstrated that BmNPV was not detected in the eggs laid by BmNPV productively infected female moths. The results indicated that BmNPV could not be transovarially transmitted.

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