• Korean Journal of Animal Reproduction
• /
• v.21 no.1
• /
• pp.25-30
• /
• 1997

This study was done to find out the preservation possibility of liquid boar semen at variabel room temperature of 9 to 16$^{\circ}C$. The percentages of sperm motility and NAR acrosome were highest in B tschwiler extender compared to B tschwiler＋Hepes, Andro＋Hepes and Andro extenders. The extenders with Hepes buffer showed detrimental effect for preservation of liquid boar semen. The pH of ejaculated sperm-rich fraction was 7.5. The pH of B tschwiler＋Hepes, B tschwiler, Andro＋Hepes and Andro extenders was 6.9, 7.5, 7.1 and 8.1, respectively. The pH of liquid boar semen with B tschwiler＋Hepes, B tschwiler, Andro＋Hepes and Andro extenders was 6.6, 6.9, 6.7 and 6.9 at 1st day of storage, and 5.5, 5.7, 5.6 and 5.8 at 7th day of storage, respectively. Gilts and sows were inseminated twice with liquid boar semen stored at 9~16$^{\circ}C$ in B tschwiler extender for 3~4 days. Farrowing rate, litter size and average pig weight at birth between AI and natural service did not differ significantly in gilt and sow, respectively. However, sow showed higher farrowing rate and litter size compared to gilt both in AI and in natural service. As a result of this study, we found out that liquid boar semen can be stored for 5~7 days at uncontrolled room temperature of 9~16$^{\circ}C$ in B tschwiler extender.

• Journal of Embryo Transfer
• /
• v.17 no.2
• /
• pp.91-100
• /
• 2002

The experiment was divided into two phases. In phase-I fresh embryos were transferred and in Phase-II frozen embryos were transferred. Embryos were collected by using Dulbecco's phosphate buffered saline. In phase-I total of 65 ova were collected out of 107 ovulation in 18 goats. Recovery of ova was 60.74％, of which 51 (78.46％) was fertilized. Sixteen embryos were transferred to 10 recipient goats and kidding was observed in 6 goats, that produced 10 kids. Thus, 62.50％ embryo survival and 60％ kidding were achieved in phase-I. In phase-II of the experiment, 17 regular cyclic Black Bengal goats were used. The main purpose was to study the viability of caprine embryos after cryopreservation. In this phase the embryos were collected and frozen using Bio-cool freezers. A two step addition of cryoprotectants (5％ glycerol and 10％ glycerol) and three-step dilution of cryoprotectants with 1mole (M) sucrose was used. Embryos were preserved for 10 to 45 days. Out of 27 embryos preserved, 18 were recovered after freezing and thawing (37$^{\circ}C$ water bath) with 33.33％ embryonic loss. Seventeen frozen and thawed embryos were transferred in 9 recipient goats, out of which kidding was observed in 6 goats and 7 kids were produced, giving a 66.66％ kidding and embryo survival of 41.17％. The technique utilized for fresh and frozen embryo transfer can be successfully utilized to produce goats of superior genetic merits. The protocol used for addition of cryoprotectant, freezing, thawing and dilution was found suitable for caprine embryo freezing.

• Korean Journal of Agricultural Science
• /
• v.2 no.1
• /
• pp.241-255
• /
• 1975

This experiment was conducted to observe the effects of fermented feed by Aspergillus oryzae and Aspergillus niger on the improvement of feed value and the effect of fermented feed additive for meat production of broiler. The results of fermented feed on the improvement of feed value were as follows; I; The effects of fermented feed value improvement were as follaws; 1) There were little difference between fermented feeds by Asp. oryzae and Asp. niger, compared with wheat bran, crude protein contents of Koji was highly increased and its nitrogen free extract and crude fat contents were decreased, but crude fiber and ash were little difference. 2) Total amino acids were highly increased as to fermented feeds but proline in Asp. niger koji feed, and proline and valine in Asp. oryzae koji feed were decreased and other amino acid were increased 2) The effect of fermented feeds on meat production of broiler were as follows; 1) Fermented feeds groups appeared higher weight (p<0.01)than weight of control on end of experimental period, but little difference were recognized between 5% and 10% fermented feed groups. 2) On the weight gain per day, highly significant were recognized(p<0.05) between control and test groups, 10% Asp. oryzae koji group was highest ($12.15{\pm}0.46g$) between all groups. 3) On the yield of carcass, there were significant highly difference (p<0.01) between control and test groups but little difference were recognized between each of 5% groups and 10% groups of fermented feeds. 4) Fermented feed groups appeared higher carcass yield (p (0<0.05) than control. But between all fermented feed groups were a little difference in partly. 5) On the influence of fowl meat composition, amount of moisture contents was a little decrease in fermented feed groups, and crude protein and crude fat were increased. 6) Feed conversion rate resulted a little amount decreasing. Specially, 10% Asp oryzae koji group was lowest (2.89) compare with control (3.35) 3. As a result of economical analysis appeared highest low income in koji groups. Low income were more gained percent of 40.22 in 10% Asp oryzae koji and 33.19 in 10% Asp. niger koji than control.

• Journal of Animal Science and Technology
• /
• v.63 no.4
• /
• pp.693-724
• /
• 2021

The in-vitro meat is a novel concept in food biotechnology comprising field of tissue engineering and cellular agriculture. It involves production of edible biomass by in-vitro culture of stem cells harvested from the muscle of live animals by self-organizing or scaffolding methodology. It is considered as efficient, environmental friendly, better ensuring public safety and nutritional security, as well as ethical way of producing meat. Source of stem cells, media ingredients, supply of large size bioreactors, skilled manpower, sanitary requirements, production of products with similar sensory and textural attributes as of conventional meat, consumer acceptance, and proper set up of regulatory framework are challenges faced in commercialization and consumer acceptance of in-vitro meat. To realize any perceivable change in various socio-economic and environmental spheres, the technology should be commercialized and should be cost-effective as conventional meat and widely accepted among consumers. The new challenges of increasing demand of meat with the increasing population could be fulfill by the establishment of in-vitro meat production at large scale and its popularization. The adoption of in-vitro meat production at an industrial scale will lead to self-sufficiency in the developed world.

• Asian-Australasian Journal of Animal Sciences
• /
• v.10 no.1
• /
• pp.98-105
• /
• 1997

Sudax fodder (Sorghum sudanense ${\times}$ Sorhum vulgare) was ensiled in laboratory silos with or without 20, 30, or 40 percent broiler litter and 6 percent molasses with or without Candida yeast. The samples were analyzed for pH, lactic acid and nitrogen fractions at the start of the experiment and at 5 days interval, thereafter till 40 days. A sharp decline in pH and increase in lactic acid content was observed on fifth day of ensiling. Thereafter, the rate of pH decline decreased till 20 days and that of lactic acid increase till 25 days and the remained constant. Increasing levels of broiler litter had adverse effect on pH drop and lactic acid increase of silages. Total-N content of the silages had little variation throughout the ensiling period. A sharp decline in protein-N and increase in ammonia-N content was observed on day 5 of ensiling. Thereafter, the content of protein-N increased till 20 days and that of ammonia-N decreased till 15 days, but these changes were very small compared to that occurred during the first 5 days of ensiling. The level of broiler litter had inverse relationship with protein degradation and direct relationship with ammonia production. The yeast inoculum failed to produce any significant effect.

• Animal Bioscience
• /
• v.37 no.5
• /
• pp.929-943
• /
• 2024

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results: The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p<0.05) reduced the lipid droplets and TG content, and their down-regulation (siFGF10) increased the measurement of lipid droplets and TG in differentiated bovine adipocytes. Furthermore, the overexpression of the FGF10 gene down regulated the mRNA levels of adipogenic marker genes such as CCAAT enhancer binding protein alpha (C/EBPα), fatty acid binding protein (FABP4), peroxisome proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), and Fas cell surface death receptor (FAS), similarly, down-regulation of the FGF10 gene enriched the mRNA levels of C/EBPα, PPARγ, FABP4, and LPL genes (p<0.01). Additionally, the protein levels of PPARγ and FABP4 were reduced (p<0.05) in adipocytes infected with Ad-FGF10 gene and enriched in adipocytes transfected with siFGF10. Moreover, a total of 1,774 differentially expressed genes (DEGs) including 157 up regulated and 1,617 down regulated genes were explored in adipocytes infected with Ad-FGF10 or Ad-NC through deep RNA-sequencing. The top Kyoto encyclopedia of genes and genomes pathways regulated through DEGs were the PPAR signaling pathway, cell cycle, base excision repair, DNA replication, apoptosis, and regulation of lipolysis in adipocytes. Conclusion: Therefore, we can conclude that the FGF10 gene is a negative regulator of bovine adipogenesis and could be used as a candidate gene in marker-assisted selection.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.1
• /
• pp.36-42
• /
• 2013

The average twin lambing rate of Bayanbulak sheep is 2% to 3%. However, a flock of sheep with a close genetic relationship and an average of 2 to 3 lambs per birth has been found recently. To determine the major genes controlling the prolificacy of the flock in the present study, the flock was designated A while 100 normal Bayanbulak sheep were randomly selected to comprise the control flock B. Ligase detection reaction method was applied to detect and analyze the 10 mutational loci of the 3 candidate prolificacy genes including bone morphogenetic protein type I receptors, bone morphogenetic protein 15, and growth differentiation factor 9. The 10 mutational loci are as follows: FecB locus of the BMPR-IB gene; $FecX^I$, $FecX^B$, $FecX^L$, $FecX^H$, $FecX^G$, and $FecX^R$ of the BMP15 gene; and G1, G8, and FecTT of the GDF9 gene. Two mutations including BMPR-IB/FecB and GDF9/G1 were found in Bayanbulak sheep. Independence test results of the two flocks demonstrate that the FecB locus has a significant effect on the lambing number of Bayanbulak sheep. However, the mutation frequency of the G1 locus in GDF9 is very low. Independence test results demonstrate that the GDF9 locus does not have a significant impact on the lambing performance of Bayanbulak sheep. Among the 10 detected loci, BMPR-IB/FecB is the major gene that influences the high lambing rate of Bayanbulak sheep.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.2
• /
• pp.216-224
• /
• 2009

In Japan, since rice consumption has been decreasing with the westernization of Japanese eating habits, surplus paddy fields have been increasing. If these surplus paddy fields can be utilized for forage rice production as feed for animal production and excretions (feces and urine) from animal production can be applied to the paddy fields as manure, then the problems of surplus paddy fields and excretions from animal production may be solved, and the environment kept sustainable. The objectives of the present study were to apply a bio-economic model to dairy and forage rice integration systems in Japan and to examine the merit of introducing whole crop rice silage (WCRS), as well as economic and environmental effects of various economic and management options in the systems. Five simulations were conducted using this model. The use of WCRS as a home-grown feed increased environmental loads and decreased economic benefit because of the higher amount of purchased feed, when compared to the use of typical crops such as maize, alfalfa and timothy silage (simulation 1). Higher economic benefits from higher forage rice yields and higher milk production of a dairy cow were obtained (simulations 2, 3). There were no economic and environmental incentives for utilizing crude protein (CP) rich WCRS, because an increase in the CP content in WCRS led to the use of more chemical fertilizers, resulting in high production costs and nitrogen outputs (simulation 4). When evaluated under the situation of a fixed herd size, increasing forage rice yields decreased the total benefit of the production, in spite of the fact that the amount of subsidies per unit of land increased (simulation 5). It was indicated that excess subsidy support may not promote yield of forage rice. It was, however, observed in most cases that dairy and forage rice integration systems could not be economically established without subsidies.

• Journal of the korean veterinary medical association
• /
• v.7 no.1
• /
• pp.74-77
• /
• 1963

The histological and cytological characteristics of pancreatic islets of chickens were studied along with the distribution and size of islets and weight of chicken pancreas versus body weight. The following is a summarg of the present work. 1. The weight

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.4
• /
• pp.523-531
• /
• 2008

Buffalo are a major source of milk production, contributing 12.1% in the World and 38.0% in Asia. The buffaloes are kept under peri-urban farming systems to produce milk for urban populations. Breeding is delayed in these herds to get more economic benefit because farmers believe that the pregnancy decreases milk production. The lactation milk value has been studied in this paper as an economic indicator. Complete milk yield records of 3,304 buffaloes was collected from a group of state farms. Economic traits including lactation yield, lactation length, calving interval (CI), dry period and milk yield per day of calving interval (MYPDCI) were derived from the data. The animals were grouped according to parity number (1-3), service period (G1 to G4, conceiving during <150, 150-200, 200-300 and >300 days post calving) and yield levels (HMY>2,500; MMY 2,001-2,500; and LMY 1,500-2,000 liters/ lactation). To study the effect of pregnancy on milk composition a research trial was conducted at a medium size private dairy farm, using forty lactating buffaloes of three yield levels and four service period groups, as described already. Milk was sampled on alternate weeks and analyzed for fat and protein contents (%). For quantifying the value of milk produced during a lactation period, the value corrected milk (VCM) was determined and converted to lactation milk value (LMV). Group means were compared for varicous parameters. Highest milk yield ($2,836.50{\pm}15.68$ liters/lactation) was recorded in the HMY animals of G4 group while lowest milk yield of $1,657.04{\pm}8.34$ liters/lactation was found in LMY of G1. Lactation was significantly increased with the extending of service period. The shortest dry period was recorded in HMY, parity 1, G1 animals and the longest in parity 2, MMY, G4.The CI was shortest in HMY, parity 1, and G1 animals and longest in LMY, parity 3, G4 buffaloes. The HMY, parity 2, G1 buffaloes showed the highest MYPDCI and the lowest value was recorded ($6.53{\pm}0.17$ vs. $2.76{\pm}0.04$ liter/day) for LMY, parity 1, G4 buffaloes. The VCM decreased with the delayed conception. This decreasing trend was higher in respect of the total yield but decrease in the VCM was smaller due to the increasing levels of fat and protein in the milk. The gap between the various production classes was reduced based on the VCM as compared with the yield per day of CI. LMV showed a consistent decline with extending service period in all three production groups. The study suggests that CI increased with delayed conception, showing a consistent trend in the low, moderate and high yielding buffaloes. There was a coherent declining pattern of milk yield with delaying conception, associated with prolonged CI. An animal conceiving at a later stage of lactation showed a decline in financial returns of 24 to 27% compared with those conceiving earlier.