• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.84-85
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• 2005

• Journal of Biosystems Engineering
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• v.24 no.6
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• pp.473-478
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• 1999

Recently, it is necessary fir surface materials to be low cost and to keep well endurable and wear in machinery field. Since most good materials with endurance and wear are expensive, they are being studied hard to modify to surface materials with endurance and wear, which overlay with the surface membrane completely. One of them is PTA(Plasma Transferred Arc Overlaying Process) method, which gets into the spotlight. It is thought to be an optimum method, since this method of overlays properly materials with about 3∼5cm surface membrane. It is necessity for the modified materials with endurance and wear to improve within the agricultural environment. Therefore, this research was used the PTA method to endure and wear a material. This method proved to be a reliable method for overlaying the membrane on the materials of an economical agricultural machinery.

• Mycobiology
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• v.39 no.4
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• pp.317-320
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• 2011

Honey production from approximately 1.6 million colonies owned by about 199,000 Korean beekeepers was almost 23,000 metric tons in 2009. Nosema causes significant losses in honey production and the virus decreases population size. We initiated a survey of honey bee colonies on the blooming period of Acacia to determine the prevalence of Nosema and virus in 2011. Most Korean beekeepers have moved from the south to north of Korea to get Acacia nectar for 2 mon. This provided a valuable opportunity to sample bees originating from diverse areas in one location. Twenty hives owned by 18 beekeepers were sampled in this year. Nosema spore counts ranged from zero to 1,710,000 spores per bee. The average number of nosema spores per bee was 580,000. Approximately 95% of the colonies were infected with Nosema, based on the presence of spores in the flowering period of Acacia. This indicates that Nosema is the predominant species affecting honeybee colonies. Also, the seven most important honeybee viruses were investigated by reverse transcription-PCR. Among them, four different viruses were detected in samples. Black queen cell virus was present in all samples. Chronic bee paralysis virus was detected in 10% of samples. Deformed wing virus was present in only 5% of the samples. Prevalence of Sacbrood virus was 15%. However, Cloudy wing virus, Israel acute paralysis virus and kashmir bee virus were not detected in any of samples.

• Journal of Apiculture
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• v.33 no.4
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• pp.277-282
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• 2018

Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on $EpiDerm^{TM}$, a reconstituted three-dimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study under the given conditions honeypolis showed no irritant effects. Honeypolis meets acceptance criteria if: mean absolute OD 570 nm of the three negative control tissues is ${\geq}0.8$ and ${\leq}2.8$, mean relative tissue viability of the three positive control tissues is ${\leq}20%$, standard deviation of relative tissue viability obtained from each three concurrently tested tissues is ${\leq}18%$. Honeypolis is therefore classified as "non-irritant" in accordance with UN GHS "No Category".

• Agricultural and Biosystems Engineering
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• v.5 no.2
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• pp.50-54
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• 2004

The study aimed to develop a mechanized mushroom growing system for the substrate materials mixing and wetting, materials fermentation and pasteurisation through the design of integrated cultivation machine system for oyster mushroom. The power requirement of the prototype during fermentation operation was measured in the range of 31$\~$33 kg-m and the torque has not so much differ by the kinds and quantity of materials. The work efficiency of conventional method for stacking the heap and turning the heap of cotton waste by tractor rotavator and manual wetting required 78 hours. But the watering, fermentation and sterilisation by the prototype use same operation required 25.5 hours, which can save the operation labour by $67\%$. The machine can be saved the requirement of heating energy by $63\%$, and the machine can also be saved the material cost by $44\%$. It is envisaged that the machine can effectively be used for large mushroom growing farms or joint use mushroom growing group-farmers in a village.

• International Journal of Industrial Entomology and Biomaterials
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• v.24 no.1
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• pp.1-5
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• 2012

A newly developed silkworm variety, GoldenSilk makes bright yellow cocoon. In this article, the basic characteristics of GoldenSilk cocoon were examined through color analysis, degumming characteristics and UV spectrophotometer. The colorant of GoldenSilk cocoon was not distributed evenly throughout the cocoon layer but existed in the outer layer of the cocoon. The colorant has not good resistance against sunlight. The degumming loss percentage of GoldenSilk cocoon was 29.2%, which is relatively higher than that of the authorized one. UV spectrophotometer showed specific absorption band around 400 to 500 nm, which is the specific yellow colorant of the GoldenSilk cocoon extracted to the degumming solution during the degumming process.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.1103-1109
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• 2010

In this paper in order to analyze the mechanical properties, the permeability and the freezing properties of SB-2 materials which are mainly used with the subbase materials on the rod. For this ends, a series of the physical test, the permeability test and the freezing test were carried out the samples mixed the small aggregate and the big aggregate from which was re-classified the SB-2. From the test results, it was analyzed the characteristics of permeability and the characteristics of freezing of the samples.

• International Journal of Industrial Entomology and Biomaterials
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• v.42 no.1
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• pp.14-20
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• 2021

Mulberry was dried with low temperature vacuum dryer using microwave. The nutritional compositions of microwave-dried mulberry including proximate composition, sugar content, mineral content, total phenolic, flavonoids, and anthocyanin, beta-carotene, vitamin C, and amino acid composition were measured. Sugar contents of mulberry were 42.6 mg/100g (Cheongilppong) and 43.27 mg/100g dw (Gwasang No. 2). The main components of mulberry sugars were fructose and glucose. Mineral analysis showed that K, P, Ca, and Mg were abundant regardless of mulberry cultivars.

• Journal of Ginseng Research
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• v.44 no.1
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• pp.123-134
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• 2020

Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused huge economic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used for the control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypic pesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins (PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary to reveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assembly method. Results: PDS treatment could cause the changes of many gene expressions which regulate its signal pathways. The genes in peroxisome proliferator-activated receptor (PPAR) signaling pathway were significantly downregulated, and then, the downstream fatty acid degradation pathway had also been greatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACB is because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR, and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL, SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradation pathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression of genes associated with catalytic activity, such as cytochrome P450 and other similar genes.

• Animal Bioscience
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• v.34 no.9
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• pp.1559-1568
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• 2021

Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.