• Journal of Applied Biological Chemistry
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• 제53권3호
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• pp.132-138
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• 2010

B. pseudomycoides로 부터 alanine racemase로 추정되는 유전자를 분리한 다음 6xHistidine 이 결합된 pET-21 운반체를 이용하여 E. coli BL21(DE3)에서 발현시키고 생화학 특성을 조사하였다. 재조합된 alanine racemase는 affinity chromatography를 이용하여 분리하였으며 SDS-PAGE 분석에서 약 46 kDa의 단일밴드를 나타내었다. 분리된 효소는 여러 아미노산 중에서 L-alanine에 대하여 가장 높은 활성도를 보이고 D-alanine에 대하여 두번째로 높은 활성도를 보였다. 따라서 분리된 효소는 alanine racemase로 판단되었다. 분리된 효소는 D-cysteine에 하여 상당히 저해가 되었다. 효소의 최적 활성도는 pH 9.0 근처에서 관찰되었고 산성의 조건보다는 알칼리의 조건에서 보다 안정하였다. 보효소인 PLP 0.3mM의 첨가에 의해 효소의 활성도는 약 70% 가량 증대되었다.

• 한국농공학회논문집
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• 제49권4호
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• pp.35-41
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• 2007

The most agricultural concrete structures for the irrigation and drainage are exposed to the underwater condition at the irrigation period and they take the influence on very severe cold in the winter. Therefore, it is impossible to use repair materials used to the general concrete structures. The research need the development of the repair material for a performance enhance of the agricultural underwater concrete structures. This research evaluated the mechanical and durability performance of the latex modified repair mortar for underwater concrete structures which peformed the repair in the underwater according to the characteristic of the agricultural concrete structure. The latex modified repair mortar is a material that minimize the effect of the ecosystem, environment and the segregation. In this research, the construction condition of the latex modified repair mortar for agricultural concrete structures was considered and the test specimens made in the underwater condition. Test results was then compared with target performance and commercial repair mortar. Experimental test results indicated that the mechanical and durability performance of latex modified repair mortar for agricultural underwater concrete structure satisfied all target performance. Also, the latex modified repair mortar resulted in better repair performance than the commercial repair mortar.

• 한국유기농업학회지
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• 제19권spc호
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• pp.135-138
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• 2011

In traditional organic rice culture practices, control of weeds is a big problem. This study was conducted to increase the rice production. Results showed that Plant height, SPAD, root length and weight were higher in pot raised seedling than broadcasting method except number of panicles. High plant density caused reduction in plant height, number of culm and chlorophyll content of the rice. No lodging was observed in both methods. Thickness of third internode and culm length was more in pot raised seedlings at both sites. When organic material was used rice yield increased by 3.81%. Higher rice production (10%) was recorded in the fields planted with pot raised seedlings. From the above study it could be conclude that the pot raised seedlings perform better than the seedlings raised by broadcasting methods.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.3105-3105
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• 2001

Ingests and fecal contamination on a poultry carcass is a food safety hazard due to potential microbiological contamination. A visible/near-infrared (NIR) spectrometer was used to discriminate among pure ingesta and fecal material, breast skin contaminated with ingesta or fecal material and uncontaminated breast skin. Birds were fed isocaloric diets formulated with either maize, mile, or wheat and soybean meal for protein requirements. Following completion of the feeding period (14 days), the birds were humanely processed and eviscerated to obtain ingests from the crop or proventriculus and feces from the duodenum, ceca, and colon portion of the digestive tract. Pure feces and ingesta, breast skin, and contaminated breast skin were scanned from 400 to 2500 nm and analyzed from 400 to 900 nm. Principal component analysis (PCA) of reflectance spectra was used to discriminate between contaminates and uncontaminated breast skin. Results indicate that visible (400 to 760 nm) and NIR 760-900 nm spectra can detect contaminates. From PCA analysis, key wavelengths were identified for discrimination of uncontaminated skin from contaminates based the evaluation of loadings weights.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권1호
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• pp.7-12
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• 2022

Farming practices that balance environmental friendliness with biodiversity are increasingly valuable. Wild plants on farmlands compete for nutrients with crops and create a crucial microhabitat and resources for animals such as natural enemies. Investigating farmlands and their surrounding plants with limited human and material resources has become an essential aspect of evaluating the agricultural ecosystem services. This study investigated plants in six agricultural long-term ecological research sites in Taiwan from 2017 to 2020 to determine the ideal season for investigation. Cluster analysis was performed to group habitats with similar plant composition, and the species-area curves of the clusters in each season were created. The results indicated that the agricultural ecosystem could be divided into farmlands, banks, orchards, and tea gardens. The habitats were divided into farmland, bank, Chia-Yi orchard, Gu-Keng orchard, and tea garden clusters. Ground plant cover can be investigated all year with at least 18 quadrats. However, if human and material resources are limited, 10 quadrats should be the minimum for farmlands in autumn and for the other microhabitats in spring. The minimum number of quadrats is 10 for banks, 17 for orchards, and 9 for tea gardens.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2003년도 추계 학술발표회 요지
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• pp.60-61
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• 2003

• Animal Bioscience
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• 제34권9호
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• pp.1559-1568
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• 2021

Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.

• 한국농공학회논문집
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• 제50권1호
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• pp.95-106
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• 2008

Eco-friendly material and implementation methods have become important along with the existing standardized maintenance concept for the irrigation and flood control. However, the intrinsic function of the hydraulic structure is water supply and disaster prevention. Therefore, the material and work methods should be considered both eco-friendliness, durability and safety which are prerequisite elements to maintain the engineering function. In this study eco-friendly material and work methods would be classified into the vegetation based system, eco-friendly concrete system and stone-material system according to the characteristics of material. The quality standard for durability followed KS specification and related regulations. The quality standard for safety and eco-friendliness was set after literature review, and the database was developed using the standard. The structure applicable to onsite - the eco-friendly material and work method were classified based on the function and material of the hydraulic structure. Finally, database has been established for convenient management and selection of the proper material and work method. The eco-friendly material and work method could be searched easily for the convenience of the users, and the web-based data system has been developed for continuous registration of the material and work methods to be developed in the future.

• 한국농공학회논문집
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• 제49권5호
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• pp.3-9
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• 2007

In this study, the effect of the purification materials is analyzed and tested by Flow 3D and Hydraulic model test. Three dimension numerical analysis led from the research that sees abnormal form and the size back of the water purification material conferred the flowing water conduct inside the test channel against the test condition. Comparison it analyzed the flux distribution, a water depth of the channel which establishes the water purification materials the cross section, an interval of the water purification material, a conference with general channel, it change executed. As a result, the cross section ratio of the purification materials against and a flux change from the test which it sees. The interval of the purification materials in order to prevent three dimension that follows in decrease of increase and flux must decide an interval.

• 한국농공학회논문집
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• 제57권6호
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• pp.141-152
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• 2015

Most of agricultural structures are consisted of complex components and exposed to various boundary conditions. There have been no ways to express those structures exactly for model experiment. As an alternative, 3D printer can produce any type of solid model. However, there are limited informations related to structural experiments using 3D printer. The object of this study gives the basic informations to structural engineers who try to use 3D printer for model experiment. When PLA was used as a supplier for 3D printer, the outcomes showed less heat deformation to compare with ABS. To test the material properties, two kinds of experiments (three-point flexibility test and compression test) were executed using universal testing machine. In three-point flexibility test, plastic hinge and its deformation were developed as observed in material such as steel. The behavior was in a linear elastic state, and elastic bending modulus and yield force were evaluated. In the compression test using unbraced columns with hinge-hinge boundary condition, the constant yield forces were observed regardless of different lengths in all columns with same section size, whereas the compressive elastic modulus was increased as the length of column was increased. The suggested results can be used for model experiments of various agricultural structures consisted of single material.