• 한국유기농업학회지
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• 제23권3호
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• pp.423-453
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• 2015

This study started with two questions. The first question is 'Why does organic agriculture want to be linked with cooperative?' The second is 'What is the characteristics of the mutual relationship between organic agriculture and cooperative?', especially in Wonju. Up to now, there have been various kinds of discussion about cooperation between organic agricultural campaign and cooperative in Wonju. But there are few concrete data showing what role cooperative plays in the cyclical process of production-distribution-consumption of organic agriculture. Thus, we conducted a survey applying the Delphi technique to the 35 organic agricultural specialists active in Wonju. First, small-scale family farm producers established a social economic cooperative network in around 2003 to perform 'the activities of innovators' through forming an organization. Producers moved from the stage in which they were supported one-sidedly by consumers to the stage where they could 'lead' cooperation. Each farm organization built a vertical integration, and horizontal cooperative systems with other farm organizations. But, practical cooperative activities have remained in the doldrums. They tend to show trends deviating from the principles of organic agriculture and cooperative. Second, the relationship between producers and consumers in Wonju can be described as 'producer-consumer cooperative type'. In Wonju, the producers' organization is not a sub-contractor which simply deliver organic agricultural products to producers' cooperative, but is an equal subject which creates its own value chain. The cooperative (cooperative organization) leads distribution and consumption of organic agricultural products and mutual communication. But, the closeness between producers and consumers has got loosened. And, it is problematic that the proportion of local food consumption in Wonju is very low. There is a very strong tendency to ride the central distribution system of producers' cooperative coalition. Consequently, it is necessary for producers' cooperatives in Wonju to run a local food distribution system based on the 'cooperative system among cooperatives'.

• BMB Reports
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• 제39권1호
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• pp.22-25
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• 2006

The gene encoding MPB83 from Mycobacterium bovis Vallee111 chromosomal DNA was amplified by using polymerase chain reaction (PCR) technique, and the PCR product was approximately 600bp DNA segment. Using T-A cloning technique, the PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-83 was constructed successfully. pGEM-T-83 and pET28a(+) were digested by BamHI and EcoRI double enzymes. The purified MPB83 gene was subcloned into the expression vector pET28a(+), and the prokaryotic expression vector pET28a-83 was constructed. Plasmid containing pET28a-83 was transformed into competence Escherichia coli BL21 (DE3). The bacterium was induced by isopropyl-$\beta$-D-thiogalactopyranoside (IPTG) and its lysates were loaded directly onto sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), approximately 26 kDa exogenous protein was observed on the SDS-PAGE. The protein was analyzed using Western-blotting. The results indicated that the protein was of antigenic activity of M. bovis. The results were expected to lay foundation for further studies on the subunit vaccine and DNA vaccine of MPB83 gene in their prevention against bovine tuberculosis.

• Asian-Australasian Journal of Animal Sciences
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• 제6권2호
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• pp.197-203
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• 1993

The protection of dry matter and nitrogen from rumen degradation of dhaincha seed (Sesbania aculeate) meal was studied using nylon bag technique. The dhaincha seed meal was subjected to various heat treatments that included oven drying, autoclaving and boiling. Similar experiment was conducted with fish meal as reference for comparison. The oven-dried dhaincha meal was found to retain more dry matter and nitrogen than was found boiled or autoclaved meal. While autoclaving appeared to improve nitrogen and dry matter retention to some extent, boiling seemed to cause more loss of dry matter from nylon bag. Heat treatment caused high retention of nitrogen by fish meal. The calculated effective protein degradation was 80.4% and 83.2% for the oven dried fish meal and dhaincha seed meal whereas same values were 74.2% and 86.7% for autoclaved fish and dhaincha seed meal respectively at the outflow rate of 4.4% per hour. The in vitro study revealed higher digestibility for heat treated samples by pepsin. The dry matter, nitrogen and ash content of dhaincha seed meal were 85.93%, 5.93% and 7.31% respectively.

• 농업과학연구
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• 제47권4호
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• pp.1097-1107
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• 2020

Assessment of soil carbon stock is essential for climate change mitigation and soil fertility. The digital soil mapping (DSM) is well known as a general technique to estimate the soil carbon stocks and upgrade previous soil maps. The aim of this study is to calculate the soil carbon stock in the top soil layer (0 to 30 cm) in Jeolla Province of South Korea using the DSM technique. To predict spatial carbon stock, we used Cubist, which a data-mining algorithm model base on tree regression. Soil samples (130 in total) were collected from three depths (0 to 10 cm, 10 to 20 cm, 20 to 30 cm) considering spatial distribution in Jeolla Province. These data were randomly divided into two sets for model calibration (70%) and validation (30%). The results showed that clay content, topographic wetness index (TWI), and digital elevation model (DEM) were the most important environmental covariate predictors of soil carbon stock. The predicted average soil carbon density was 3.88 kg·m-2. The R2 value representing the model's performance was 0.6, which was relatively high compared to a previous study. The total soil carbon stocks at a depth of 0 to 30 cm in Jeolla Province were estimated to be about 81 megatons.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.443-452
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• 1993

Direct freezing tests of soybean seed by liquid nitrogen were carried out at various moisture contents and the following important conclusions were drawn from the results of temperature measurements of soybean seed and photographs of bubbles generated on its surface : 1) Assuming that the temperature gradient in a soybean seed is negligible because of its small seed size and the freezing ratio is followed the Heiss's formula, and a differential equation based on the heat energy balance was introduced . The equation was easily solved by the Runge-Kutta-Gill method and the predicted values of the temperature were in good agreement with the observed data. 2) The photographs of bubble generation during freezing showed the boiling mode was nucleate, and then the most suitable formula on the nucleate boiling heat transfer was introduced from many formulate proposed up to now by fitting the calculated values based on the formula to the observed data. The formula used for the predict on of the seed temperature was as follows: $\frac{{\partial}T_s}{\partial\theta}\;=\;-\frac{{\alpha}(T_s\;-\;T_L)^{3.3}}{W(C_s\;-\;\frac{{\delta}m(CT_s\;+\;{\sigma})}{T_s^2})}$ where C = difference of the specific heat between pure ice and water m=moisture content of soybean seed $T_s$ = seed temperature $T_L$ = Temperature of liquid nitrogen W = mass of soybean seed $\alpha$ = proportional constant $\delta$ = constant depends on variety or the type of seed $\theta$ = time $\sigma$ = latent heat of melting of pure ice This study will give important information in the hydro-freezing technique by liquid nitrogen, available as a new technique of processing agricultural products in the near future.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.227-233
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• 2017

Purpose: Morphological properties of soybean roots are important indicators of the vigor of the seed, which determines the survival rate of the seedlings grown. The current vigor test for soybean seeds is manual measurement with the human eye. This study describes an application of a machine vision technique for rapid measurement of soybean seed vigor to replace the time-consuming and labor-intensive conventional method. Methods: A CCD camera was used to obtain color images of seeds during germination. Image processing techniques were used to obtain root segmentation. The various morphological parameters, such as primary root length, total root length, total surface area, average diameter, and branching points of roots were calculated from a root skeleton image using a customized pixel-based image processing algorithm. Results: The measurement accuracy of the machine vision system ranged from 92.6% to 98.8%, with accuracies of 96.2% for primary root length and 96.4% for total root length, compared to manual measurement. The correlation coefficient for each measurement was 0.999 with a standard error of prediction of 1.16 mm for primary root length and 0.97 mm for total root length. Conclusions: The developed machine vision system showed good performance for the morphological measurement of soybean roots. This image analysis algorithm, combined with a simple color camera, can be used as an alternative to the conventional seed vigor test method.

• 농촌지도와개발
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• 제23권3호
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• pp.309-320
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• 2016

This study has systematically classified established the agricultural management risks from the agricultural company's position and analyzed their counterplan situation in order to seek measures to counteract this. The AHP & IPA technique were used as an analysis method concerning the evaluation results. The counterplan situation was deduced through an interview investigation of 112 farmers who produce cabbage, white radish, onion, garlic, chili, and 6 other such items in order to stereotype the risks. If the agricultural companies' management capacity is raised in accordance to the risk counterplan deduced from this study, and the risks of agricultural management activity are avoided ahead of time or counter means are activated, we can anticipate the stabilization of agricultural management.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.213-219
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• 2000

Two experiments were conducted to determine ileal digestibilities for the amino acids contained in sesame meal using the regression technique and then applying the values obtained in a growth trial using growing-finishing pigs. For the digestibility trial, four, 20 kg crossbred (Yorkshire $\times$ Landrace $\times$ Beijing Black) barrows were fitted with a simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75 percent sesame meal. For the growth trial, 210 crossbred (Yorkshire $\times$ Landrace $\times$ Henan Min) growing pigs ($21.8{\pm}1.4kg$), were fed corn-soybean meal based diets supplemented with 0, 3, 6, 9, or 12% sesame meal. Three pens (7 gilts and 7 castrates) were assigned to each treatment. With the exception of arginine and phenylalanine, the digestibility coefficients for the indispensible amino acids declined as the level of sesame meal in the diet increased. There was little agreement between the amino acid digestibilities determined with the regression technique and values previously published for sesame meal determined with the direct method. Daily gain and feed conversion both declined (linear effect p=0.02 and 0.06 respectively) as the level of sesame meal in the diet increased.

• Journal of Biosystems Engineering
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• 제41권3호
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• pp.208-220
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• 2016

Purpose: Fusel oil is a potent volatile aroma compound found in many alcoholic beverages. At low concentrations, it makes an essential contribution to the flavor and aroma of fermented alcoholic beverages, while at high concentrations, it induced an off-flavor and is thought to cause undesirable side effects. In this work, we introduce Fourier transform near-infrared (FT-NIR) spectroscopy as a rapid and nondestructive technique for the quantitative determination of fusel oil in the Korean alcoholic beverage "soju". Methods: FT-NIR transmittance spectra in the 1000-2500 nm region were collected for 120 soju samples with fusel oil concentrations ranging from 0 to 1400 ppm. The calibration and validation data sets were designed using data from 75 and 45 samples, respectively. The net analyte signal (NAS) was used as a preprocessing method before the application of the partial least-square regression (PLSR) and principal component regression (PCR) methods for predicting fusel oil concentration. A novel variable selection method was adopted to determine the most informative spectral variables to minimize the effect of nonmodeled interferences. Finally, the efficiency of the developed technique was evaluated with two different validation sets. Results: The results revealed that the NAS-PLSR model with selected variables ($R^2_{\upsilon}=0.95$, RMSEV = 100ppm) did not outperform the NAS-PCR model (($R^2_{\upsilon}=0.97$, RMSEV = 7 8.9ppm). In addition, the NAS-PCR shows a better recovery for validation set 2 and a lower relative error for validation set 3 than the NAS-PLSR model. Conclusion: The experimental results indicate that the proposed technique could be an alternative to conventional methods for the quantitative determination of fusel oil in alcoholic beverages and has the potential for use in in-line process control.

• 농업과학연구
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• 제47권4호
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• pp.933-940
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• 2020

Land-use change matrix data is important for calculating the LULUCF (land use, land use change and forestry) sector of the national greenhouse gas inventory. In this study, land cover changes in 2004 and 2019 were compared using the Wall-to-Wall technique with a land cover map of Sejong City from the Ministry of Environment. Sejong City was classified into six land use classes according to the Intergovernmental Panel on Climate Change (IPCC) guidelines: Forest land, crop land, grassland, wetland, settlement and other land. The coordinate system of the land cover maps of 2004 and 2019 were harmonized and the land use was reclassified. The results indicate that during the 15 years from 2004 to 2019 forestlands and croplands decreased from 50.4% (234.2 ㎢) and 34.6% (161.0 ㎢) to 43.4% (201.7 ㎢) and 20.7% (96.2 ㎢), respectively, while Settlement and Other land area increased significantly from 8.9% (41.1 ㎢) and 1.4% (6.9 ㎢) to 35.6% (119.0 ㎢) and 6.5% (30.3 ㎢). 79.㎢ of cropland area (96.2 ㎢) in 2019 was maintained as cropland, and 8.8 ㎢, 1.7 ㎢, 0.5 ㎢, 5.4 ㎢, and 0.4 ㎢ were converted from forestland, grassland, wetland, and settlement, respectively. This research, however, is subject to several limitations. The uncertainty of the land use change matrix when using the wall-to-wall technique depends on the accuracy of the utilized land cover map. Also, the land cover maps have different resolutions and different classification criteria for each production period. Despite these limitations, creating a land use change matrix using the Wall-to-Wall technique with a Land cover map has great advantages of saving time and money.