• Title/Summary/Keyword: Agricultural R and D

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A Priority Analysis of Excellent Agricultural R&D Performance Considering Region-wide Diffusion Effect (지역파급성을 고려한 농업R&D 우수성과 순위 분석)

  • Yun, Jin-Woo;Chae, Yong-Woo;An, Yong-jin;Kim, Seong-Sup
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.471-478
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    • 2020
  • This study aimed to suggest implications for the future development of agricultural R&D through a priority analysis of excellent agricultural R&D performance considering productivity, marketability, and effects on region, which are important factors of agricultural R&D evaluation. The subjects of analysis were R&D of Rural Development Administration (RDA), which was selected as national R&D excellent Performance from 2006 to 2018, and the priority was analyzed using Analytic Hierarchy Process (AHP). The results are as follows. First, the relative importance of the criteria decreased in the order of marketability(0.423) > regional impact(0.360) > productivity(0.216). Second, as a result of analyzing the relative importance of the sub-criteria, the possibility of commercialization(0.515) was high in the category of marketability. The possibility of technology expansion(0.538) was high in the category of effects on region and the quality improvement(0.464) was high in the category of productivity. Third, the excellent agricultural R&D performance that had high relative importance was the technologies associated with improving the agricultural competitiveness of horticultural crops.

Induction of pathogenicity mutants from Elsinoe fawcettii, the causal fungus of citrus scab by genetic transformation

  • Hyun, Jae-Wook;Lee, Seung-Chan;Kim, Dong-Hwan;Kim, Kwang-Sik;Lim, Han-Chul
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.68.2-69
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    • 2003
  • Genetic transformation carried out to induce the pathogenicity mutants from the two isolates, Elsinoe fawcettii R-34 and MUD of citrus scab fungus to hygromycin resistant by transferring plasmides (pUCATPH) that contain hygB gene. We produced protoplast for transformation by using of combinations of available enzymes including ${\beta}$-D-glucanase, ${\beta}$ -glucuronidase, Iyticase and driselase. The protoplasts regenerated at 64 $\mu\textrm{g}$/ml of hygromycin B but not 128 $\mu\textrm{g}$ in sensitivity test to identify the concentration of useful marker for the selection of transformants. Approximately 1200 and 67 hygromycin resistant isolates from strain R-34 and strain MUD, respectively, were isolated on PDA added with 200 $\mu\textrm{g}$ /ml of hygromycun B. Fifty seven and 4 of hygromycin resistant isolates from strain R-34 and MUD, respectively, did not produce necrotic lesions on the leaf in detached-leaf assay. Finally, 9 isolates were isolated from strain R-34, and these Isolates produced non or very few symptoms on seedlings of citrus in greenhouse pathogenicity test. And it's very interesting that some isolates produced melanose-like symptom on very young leaves which it was not typical symptom and somtimes produced on only expanded leaf.

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Cultural Characteristics of Mycelial Growth by an Entomogenous Fungus, Cordyceps pruinosa Petch (붉은자루동충하초의 균사생육에 관한 연구)

  • Hong, In-Pyo;Nam, Sung-Hee;Jung, I-Yeon;Sung, Gyoo-Byung;Nam, Hack-Woo;Chang, Seung-Jong;Hyeon Hyur;Lee, Min-Woong;Guo, Shun-Xing
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.116-120
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    • 2003
  • This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of C. pruinosa. C. pruinosa showed the most favorable growth on the MCM medium. The optimal condition for the mycelial growth was obtained at 25$^{\circ}C$ and pH 5.0, respectively. Carbon sources such as arabinose, mannose, xylose were favorable for stimulating a mycelial growth and fruiting bodies of C. pruinosa. Ammonium nitrate, ammonium citrate and ammonium tartrate of nitrogen sources also appeared to be good in the mycelial growth and fruiting body formation.

Studies on Physiology, Ecology and Protection of Citrus Canker Caused by Xanthomonas axonopodis pv, citri

  • Lee, Seong-Chan;Hyun, Jae-Wook;Kim, Dong-Hwan;Kim, Kwang-Sik;Lim, Han-Chul
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.124-124
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    • 2003
  • Citrus canker is very important disease in international trade of citrus. The disease was usually take place from late of June, and severe middle of July to middle of August, though disease occurrence was affected by environmental conditions. In pathogenicity test, three varieties, orange, lemon and kiyomi among 7 varieties, were succeptible, two varieties, satsuma mandarin and iwasachi, intermediate resistant. On the other hand, shiranuhi and yuzu were resistant relatively. The pathogen, Xanthomonas axonopodis pv. citri, grew well in PD broth adjusted to pH 7.0 at 26$^{\circ}C$. It's growth was best in medium containing group of monosaccharide as a carbon source and group of ammonium as a nitrogen source. Tow isolates were resistant to streptomycin among 11 isolates isolated from diseased leaves in field in Jeju-Do. The streptomycin sensitives isolate was controlled by in greenhouse test. On the other hand, the resistant and sensitive isolates were controlled by treatment with copper sulfate, the control value is 88.7% and 90.6%, respectively.

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Study on Agricultural Science Convergence R&D Agenda under the Fourth Industry Revolution (4차 산업혁명 관련 농학계 융·복합 R&D 어젠다 연구)

  • Lee, Dong-Hoon;Kim, So-jin;Jang, Keum-Il;Sa, Tong-min;Yoo, Do-il
    • The Journal of the Korea Contents Association
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    • v.19 no.7
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    • pp.323-334
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    • 2019
  • The purpose of this study is to propose agenda concerning the direction of agricultural science convergence research and development (R&D) under the fourth industrial revolution. For this study, we apply the Analytic Hierarchy Process (AHP) targeted at experts in the fields of agricultural academia and research operating R&D currently. Results suggest the following agendas; first, human resource training toward future is more emphasized rather than fragmentary technology innovation. Second, a flexible road map for agricultural science R&D need to be made for responding to short and long term issues relevant to the innovation. Third, mutual exchange and cooperative system need to be constructed between academia and research in order to create synergy effects. Finally, both institutional improvement and humanistic literacy should be emphasized for rapidly changing conditions and better human life under the fourth industrial revolution.

Specific Detection of Vairimorpha spp. in Lepidoptera by Multiplex Polymerase Chain Reaction

  • Choi, Ji-Young;Je, Yeon-Ho;Kim, Jong-Gill;Choi, Young-Cheol;Kim, Won-Tae;Kim, Keun-Young
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.138-140
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    • 2003
  • We report the development of a multiplex PCR-based procedure for rapid, sensitive, and specific detection of entomopathgenic microspordia and differentiation of the genus Nosema and Vairimorpha and identification of the vairimorpha necatrix. (omitted)

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