• Title/Summary/Keyword: Agricultural Biotechnology

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Studies on QTLs for Bakanae Disease Resistance with Populations Derived from Crosses between Korean japonica Rice Varieties

  • Dong-Kyung Yoon;Chaewon Lee;Kyeong-Seong Cheon;Yunji Shin;Hyoja Oh;Jeongho Baek;Song-Lim Kim;Young-Soon Cha;Kyung-Hwan Kim;Hyeonso Ji
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.201-201
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    • 2022

  • Rice bakanae disease is a serious global threat in major rice-cultivating regions worldwide causing high yield loss. It is caused by the fungal pathogen Fusarium fujikuroi. Varying degree of resistance or susceptibility to bakanae disease had been reported among Korean japonica rice varieties. We developed a modified in vitro bakanae disease bioassay method and tested 31 Korean japonica rice varieties. Nampyeong and Samgwang varieties showed highest resistance while 14 varieties including Junam and Hopum were highly susceptible with 100% mortality rate. We carried out mapping QTLs for bakanae disease resistance with four F2:F3 populations derived from the crosses between Korean japonica rice varieties. The Kompetitive Allele-Specific PCR (KASP) markers developed in our laboratory based on the SNPs detected in Korean japonica rice varieties were used in genotyping F2 plants in the populations. We found four major QTLs on chromosome 1, 4, 6, and 9 with LOD scores of 21.4, 6.9, 6.0, and 60.3, respectively. In addition, we are doing map-based cloning of the QTLs on chromosome 1 and 9 which were found with Junam/Nampyeong F2:F3 population and Junam/Samgwang F2:F3 population, respectively. These QTLs will be very useful in developing bakanae disease resistant high quality rice varieties.

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Inheritance of Pigeonpea Sterility Mosaic Disease Resistance in Pigeonpea

  • Daspute, Abhijit;Fakrudin, B.;Bhairappanavar, Shivarudrappa B.;Kavil, S.P.;Narayana, Y.D.;Muniswamy, Muniswamy;Kaumar, Anil;Krishnaraj, P.U.;Yerimani, Abid;Khadi, B.M.
    • The Plant Pathology Journal
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    • v.30 no.2
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    • pp.188-194
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    • 2014

  • A comprehensive study was conducted using PPSMV resistant (BSMR 736) and susceptible (ICP 8863) genotypes to develop a segregating population and understand the inheritance of PPSMV resistance. The observed segregation was comparable to 13 (susceptible): 3 (resistant). Hence, the inheritance was controlled by two genes, SV1 and SV2, with inhibitory gene interaction.

  • https://doi.org/10.5423/PPJ.NT.10.2013.0104 인용 PDF KSCI

Enzymatic Synthesis of Novel $\alpha$-Amylase Inhibitors via Transglycosylation by Thermotoga maritima Glucosidase

  • Kim, Sung-Hee;Lee, Myoung-Hee;Yang, Sung-Jae;Kim, Jung-Woo;Cha, Hyun-Ju;Cha, Jae-Ho;Nguyen, Van Dao;Park, Kwan-Hwa
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.302-307
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    • 2008

  • Novel amylase inhibitors were synthesized via transglycosylation by Thermotoga maritima glucosidase (TMG). TMG hydrolyzes acarbose, acarviosine-glucose, and maltooligosaccharide by releasing $^{14}C$-labeled glucose from the reducing end of each molecule. When TMG was incubated with acarviosine-glucose (the donor) and glucose (the acceptor), two major transfer products, compounds 1 and 2, were formed via transglycosylation. The structures of the transfer products were determined using thin-layer chromatography (TLC), high-performance ion chromatography (HPIC), and $^{13}C$ nuclear magnetic resonance (NMR) spectroscopy. The results indicate that acarviosine was transferred to glucose at either C-6, to give a $\alpha-(1{\rightarrow}6$) glycosidic linkage, or at C-3, to produce an $\alpha-(1{\rightarrow}3$) glycosidic linkage. The transfer products showed a mixed-type inhibition against porcine pancreatic $\alpha$-amylase; therefore, they may be useful not only as inhibitors but also as acarbose transition-state analogs to study the mechanism of amylase inhibition.

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