• Asian-Australasian Journal of Animal Sciences
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• 제30권5호
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• pp.603-621
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• 2017

The current review focuses on characterization and conservation efforts vital for the development of breeding programmes for indigenous beef cattle genetic resources in Southern Africa. Indigenous African cattle breeds were identified and characterized using information from refereed journals, conference papers and research reports. Results of this current review reviewed that smallholder beef cattle production in Southern Africa is extensive and dominated by indigenous beef cattle strains adaptable to the local environment. The breeds include Nguni, Mashona, Tuli, Malawi Zebu, Bovino de Tete, Angoni, Landim, Barotse, Twsana and Ankole. These breeds have important functions ranging from provision of food and income to socio-economic, cultural and ecological roles. They also have adaptive traits ranging from drought tolerant, resistance to ticks and tick borne diseases, heat tolerance and resistance to trypanosomosis. Stakeholders in the conservation of beef cattle were also identified and they included farmers, national government, research institutes and universities as well as breeding companies and societies in Southern Africa. Research efforts made to evaluate threats and opportunities of indigenous beef cattle production systems, assess the contribution of indigenous cattle to household food security and income, genetically and phenotypically characterize and conserve indigenous breeds, and develop breeding programs for smallholder beef production are highlighted. Although smallholder beef cattle production in the smallholder farming systems contributes substantially to household food security and income, their productivity is hindered by several constraints that include high prevalence of diseases and parasites, limited feed availability and poor marketing. The majority of the African cattle populations remain largely uncharacterized although most of the indigenous cattle breeds have been identified.

• 한국식품영양학회지
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• 제32권3호
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• pp.216-225
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• 2019

The objective of this study was to compare nutrients intake, health indices, and prevalence of chronic diseases by occupation in middle-aged men, using results from National Health and Nutrition Examination Survey. The subjects were divided into two groups by their occupation (Office worker (OW) and Agri-fishery worker (AFW)) and their percentages were 75.5% and 24.5% respectively. In health-related factors, drinking rate was higher in OW (p<0.001), while smoking rate was higher in AFW (p<0.001). Walking and strength exercise were both higher in OW group. In dietary behavior, the rate of skipping breakfast and eating out was higher in OW (p<0.001). Food supplement usage were consumed by OW more than AFW (p<0.001). Daily energy intake was higher in AFW (p<0.05). There were significant differences in nutrient density per 1,000 kcal of minerals and vitamins between the two groups (p<0.05~p<0.001), except calcium, potassium, vitamin A and thiamin. In body weight, BMI, waist circumference and blood pressures, OW was higher than AFW (p<0.05~p<0.001). Whereas, HDL and LDL-cholesterol were higher in AFW (p<0.001). The prevalence of diabetes was higher in AFW (p<0.001). The results of this study can be used as data to establish nutrition and health strategies for occupation in middle-aged men.

• 농업과학연구
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• 제49권4호
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• pp.1067-1083
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• 2022

The objectives of this study were to classify the types of elderly households and to compare the characteristics of their dietary lifestyle. Panel data surveyed by Korea Rural Economic Institute (KREI) for Food Purchase Attitudes over three years (2019 - 2021) were utilized for the analysis. Through a factor analysis, five common factors were extracted out of 19 basic variables related to dietary style, which indicate two kinds of consumer competency index (safe diet, traditional diet) and three kinds of purchase frequency (healthy food, meat & fish, fresh seafood). Applying the cluster analysis method, by using socioeconomic variables along the five common factors, elderly households aged 60 or older were grouped into four types. As a result, Type 1 elderly households accounted for 50.8%, Type 2 for 16.2%, Type 3 for 27.8%, and Type 4 for 5.2% out of all 870 elderly households. Type 1 is characterized as a low-income vulnerable class with a poor diet, Type 2 as a middle-income class with a healthy food-oriented diet, whereas Type 3 was classified as a middle-income class with a meat-oriented diet, and Type 4 as a high-income class with diverse dietary culture. It is necessary to expand the agri-food voucher pilot project to the entire country and also increase the monthly subsidy for the Type 1 elderly households. Implementing community kitchen projects for elderly single-person households, promoting senior internships by providing incentives to companies that employ retirees, the provision of education by local governments on a safe and balanced diet for Types 2 and 3, and the promotion of an elderly-friendly social environment are also recommended.

• 생명과학회지
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• 제33권11호
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• pp.897-904
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• 2023

본 연구는 Bacillus velezensis K10의 유전체 분석결과에 따른 유전자의 고유한 특성을 이용하여 유전자마커를 탐색하고 확립하여 산업현장에서 활용하기 위해서 수행하였다. B. velezensis K10은 총 4,159,835 bps를 유지하였으며, 5,136개의 단백질을 발현하는 것으로 조사되었다. B. velezensis K10은 표준균주에 비교하여 전반적으로 외부요인에 기인되는 유전자의 이동이 훨씬 많은 것으로 나타났다. 이와 같은 양상에 기인하여 B. velezensis K10은 특유의 유전적 서열을 유지할 수 있는 것으로 추정되었다. 선발마커 발굴을 위해 recombinase, integrase, transposase, phage-related genes, 등 유전자 변이 유발이 쉬운 게놈상의 영역에 대해 탐색을 실시하였다. 그 결과, 선발마커로써 가능성이 높은 9개 부위를 확보하였다. 후보마커는 일부 다른 영역에서 특이성을 보이는 영역들이 존재했지만, phage 연관 유전자들에서 매우 특이성이 높았기 때문에, 모두 phage 관련 부위에서 모두 탐색되었다. 종간 후보 선발마커로써 B. licheniformis K12, B. velezensis K10, B. subtilis, B. cereus 등에 대해 PCR 분석을 실시하였다. 그 결과 BV3, BV5~9까지 총 6 프라이머 세트에서 B. velezensis K10 특이적인 PCR 산물이 형성되는 것을 확인하였다. 다른 한편으로, subspecies 수준에서 분석 결과, BV3, 5, 8, 9 등 4 프라이머 세트에서 B. velezensis K10 특이적인 PCR 산물이 형성되는 것을 관찰했다. 분석된 마커 중에서 BV5와 8가 가장 특이성이 높았기 때문에 종(species) 및 아종(sub-species) 수준에서 B. velezensis K10 유전자 선발마커로써 BV5와 8을 활용 가능할 것으로 제의된다.

• 생명과학회지
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• 제34권7호
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• pp.465-475
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• 2024

Lactiplantibacillus plantarum K9은 굼벵이에서 분리된 다양한 생리활성물질에 기인하여 프로바이오틱스 균주로 활용 가능한 유산균이다. L. plantarum K9 유전체 분석결과로써 박테리아 염색체와 3 plasmid가 존재하는 것으로 나타났다. L. plantarum K9의 핵심 대사경로 분석 결과 해당과정, 오탄당대사(pentose phosphate pathway)는 정상적으로 수행되는 것으로 나타났다. 그러나 포도당신생합성과 ED pathway의 핵심 효소인 fructose-1,6-bisphosphatase (EC: 3.1.3.11)와 6-phosphogluconate dehydratase (EC: 4.2.1.12) / 2-keto-de- oxy-6-phosphogluconate (KDPG) aldolase (EC: 4.2.1.55)가 각각 결여되어 있기 때문에 포도당신생합성과 ED pathway는 수행하지 못하는 것으로 제의된다. 또한, TCA 회로에서 fumarate 및 malate를 형성하는 일부 효소만 존재하는 반면에 나머지 TCA 회로에 연관되는 효소들이 모두 결여되어 있었기 때문에 TCA 회로는 진행되지 못하는 것으로 추정되었다. 산화적 전자전달계는 NADH dehydrogenase complex I과 cytochrome reductase complex IV에 해당하는 요소들을 보유하고 있기 때문에 class IIB 타입(bd-type)의 전자전달시스템을 수행할 것으로 예측되었다. 종합적으로, L. plantarum K9은 lactic acid 동형발효를 수행하며, 포도당신생합성 및 오탄당대사가 가능하며, class IIB 타입(bd-type) 산화적 전자전달시스템에 의해 에너지 대사를 수행하는 것으로 제의된다. 따라서, L. plantarum K9은 다른 유산균주에 비교하여 lactic acid 생성량이 비교적 높아 생리활성도가 우수할 것으로 제의된다. 다른 한편으로, L. plantarum K9은 산화적 전자전달이 가능한 것으로 추정되어 산소에 대한 내성이 높아서 배양 특성이 양호하여 프로바이틱스로써 활용가능성이 높은 것으로 제의된다.

• 농촌계획
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• 제18권2호
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• pp.47-56
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• 2012

Recently urban areas have been advanced in the aspects of convenience, traffic, and cultural environments, but they have faced various problems including environmental issues, traffic congestion, and increasing stress. In contrast, rural areas are in charge of various functions, conservations of natural environments and traditional cultures. Rural life style may be beneficial to urbanites. As urbanites are increasingly interested in leisure activities, such as experiences of tradition cultures and education, safe foods and rural tourism are gaining attention as alternative ways of satisfying their desires. In other words, the rural tourism not only provides urbanites with leisure spaces by playing a role in relations between urban and rural areas, but also acts as a nonfarm income to the rural residents. With the changes of time, the number of current rural experience tourism on a nationwide is increasing and competition among locations is getting intensive. Particularly, despite various rural tourism villages in operation, there is not a standard in the dice for experience cost and accommodation costs. Accordingly, the aim of the study was to estimate urbanites' willingness to pay(WTP) for rural experience tourism and to provide basic data for qualitative growth and revitalization with regard to the tours. The estimated WTP for rural experience tourism was found to be 5,600won for experience, 5,600won for food, 42,000won for accommodation, and 13,000won for purchasing farm products, respectively. This trend could be similarly found (there were slight differences in food cost) on all analyses, such as the research of the current situations of rural tourism villages in Gyeongnam province, the pilot survey and the estimation depending on whether or not urbanites experienced the tours. In other words, the WTP for urban hands-on experience tours estimated by this study is considered highly significant in terms with possibility of its application in the sites. It is concluded that the urbanites' WTP for rural experience tourism obtained by this study will contribute to the setup of standard index of rural tourism, the qualitative development of rural hands-on experiences, and the raise of nonfarm income.

• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.56-64
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• 2011

This study was conducted to evaluate the use of exogenous enzymes as a potential means of improving the ruminal digestion (i.e., degradability) of alfalfa hay and rice straw. Twenty six enzyme-additives were examined in terms of protein concentration and enzymic activities on model substrates. The exogenous enzymes contained ranges of endoglucanase, xylanase, ${\beta}$-glucanase, ${\alpha}$-amylase, and protease activities. Six of the enzyme additives were chosen for further investigation. The enzyme additives and a control without enzyme were applied to mature quality alfalfa hay substrate and subsequently incubated in rumen batch cultures. Five of the enzyme additives (CE2, CE13, CE14, CE19, and CE24) increased total gas production (GP) at 48 h of incubation compared to the control (p<0.05). The two additives (CE14 and CE24) having the greatest positive effects on alfalfa hay dry matter, neutral detergent fibre (NDF) and acid detergent fibre (ADF) degradability were further characterized for their ability to enhance degradation of low quality forages. The treatments CE14, CE24, a 50:50 combination of CE14 and CE24 (CE14+24), and control (no enzyme) were applied to mature alfalfa hay and rice straw. For alfalfa hay, application of the two enzyme additives, alone and in combination, increased GP compared to the control at 48 h fermentation (p<0.05), whereas only CE14 and CE14+24 treatments improved GP from rice straw (p<0.05). Rumen fluid volatile fatty acid concentrations throughout the incubation of rice straw were analyzed. Acetate concentration was slightly lower (p<0.05) for CE14${\times}$CE24 compared to the control, although individually, CE14 and CE24 acetate concentrations were not different from the control. Increases (p<0.05) in alfalfa hay NDF degradability measured at 12 and 48 h of incubation occurred only for CE14 (at 12 h) and for CE14+24 (at 12 and 48 h). Similarly, ADF degradability increased (p<0.05) with CE14 and CE14+24. As for rice straw, increased DM degradability was observed at 12 and 48 h of incubation for all enzyme treatments with an exception for CE14 at 12 h. The degradability of NDF was improved by all the enzyme treatments at either incubation time, while ADF degradability was only enhanced at 48 h. Overall, the enzymes led to enhanced digestion of mature alfalfa and there was evidence of improved digestibility of rice straw, an even lower quality forage.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.965-973
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• 2011

Our objective was to determine the differences in the rate and extent of dry matter digestion between barley subjected to differing agronomic variables. Two malting barley varieties, Copeland and Metcalfe were seeded at rates of 200 and 400 plants/$m^2$. Each of these varieties received nitrogen fertilizer at rates of 0, 30, 60 and 120 kg/ha, resulting in a total of 20 different barley grain samples. Samples were ground through a 6mm screen and approximately 3 g of each weighed into 50 ${\mu}m$ Dacron bags and sealed. The bags were incubated in three ruminally cannulated Holstein cattle for periods of 0, 3, 6 and 24 h. Using the data obtained from these incubations, rates of digestion were able to be predicted. The soluble fraction ranged from 0.229-0.327, the slowly degradable fraction ranged from 0.461-0.656, and the undegradable fraction ranged from 0.038-0.299. The rates of digestion ranged from 0.127-0.165 $h^{-1}$ and the effective degradability ranged from 0.527-0.757. At the Canora location, the Copeland samples which received 120 kg/ha of nitrogen fertilizer had a significantly lower (p = 0.013) soluble fraction than the rest of the samples at that location. A significant interaction (p = 0.009) was seen between the seeding rate and nitrogen fertilizer application with samples from the Canora location, as well as significant differences (p = 0.029) between nitrogen application rates in samples from the Indian head location. The rate of digestion of samples from the Indian head location differed (p = 0.020) between the two seeding rates, with samples seeded at 200 seed/$m^2$ having a slightly higher rate of degradation. Differences in the effective degradability were seen between the different nitrogen application rates with samples from both the Canora and Indian head locations, as well as an (p = 0.004) interaction between the seeding rate and nitrogen fertilizer application rate. Although there was not a clear correlation between the different variables, both nitrogen application and seeding rate did have a significant effect on the rates and extent of digestion across each of the four locations.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.929-939
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• 2011

The effects of micronization on in situ and in vitro nutrient disappearances of wheat, barley and corn were investigated in a series of experiments. In Experiment 1, chemical composition and in situ dry matter disappearance (DMD) of six varieties of wheat were determined. In addition, an in vitro study was completed using ground micronized and unmicronized wheat (var. Kansas). In Experiment 2, three varieties of wheat (Kansas, Sceptre and Laura) and in Experiment 3, three cereal grains (wheat, barley and corn) were either micronized for 1 min to attain internal kernel temperatures of 90-100$^{\circ}C$ or not (controls), and DM, protein and starch disappearances were estimated. In Experiment 2, an in vitro study was also completed using ground micronized and unmicronized wheat (var. Kansas). Wheat samples varied with respect to crude protein (10.0-21.2%), starch (61.6-73.9%), NDF (8.5-11.8%), volume weight (753-842 g/L) and kernel hardness (0.0-32.0). Rate (p = 0.003) and extent (p = 0.001) of in situ DMD differed among wheat varieties. Correlations between in situ kinetics, and chemical and physical properties of wheat varieties showed that protein content was negatively correlated with the rate of disappearance ($r^2$ = -0.77). Micronization of all grains markedly reduced (p = 0.001) the rate and extent of DM, and protein disappearances as compared to control samples. Micronization increased (p<0.05) the digestion of starch in wheat. However, release of ammonia into the incubation medium was markedly reduced (p<0.05), suggesting that micronization increased the resistance of protein to microbial digestion. Disappearances of DM, protein and starch differed (p = 0.001) among cereal grains with wheat>barley>corn. Micronization reduced the rate of DM disappearance (p = 0.011) and slowly degradable protein fractions (p = 0.03), however, increased (p = 0.004) slowly degradable starch fractions of all three cereals. Examination of in situ samples by scanning electron microscopy confirmed that microbial colonization focused on starch granules in micronized grains, and that the protein matrix exhibited resistance to microbial colonization. These results suggest that micronization may be used to increase the ruminal escape value of protein in cereal grains, but may lead to increased starch digestion if grains are finely ground.

• 한국식품영양과학회지
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• 제16권4호
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• pp.343-349
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• 1987

신선한 도시락의 취식과 식중독 위험을 덜 수 있는 도시락 보관조건을 확립하기 위하여 도시락 보관 중 밥의 온도변화를 조사하고 밥통과 찬통사이에 단열재를 끼워 보관하면서 찬의 온도변화, 김치의 pH값 변화와 밥의 호기성 세균수 및 응축수 생성 등에 미치는 영향을 조사하였다. 더운 밥을 도시락 밥통에 담았을 때 밥의 온도는 $70^{\circ}C$로 이 온도는 도시학내의 생활세포의 살균효과가 있으나 더운 도시락 위에 놓인 찬통의 온도가 $53^{\circ}C$까지 상승되어 밥의 신선도 유지에 큰 영향을 준다. 도시락밥통과 찬통 사이에 단열재를 놓은 경우 찬의 온도는 보관온도보다 $2{\sim}3^{\circ}C$ 상승하는 정도로 단열효과가 있어서 찬통 중 김치의 pH값이 단열재를 사용하지 않은 경우보다 높게 유지되었다. 밥을 퍼담고 바로 뚜껑을 닫은 경우는 10분 통안 식힌 후 뚜껑을 닿은 경우보다 호기성 세균수가 훨씬 적었고, 밥을 솥안에서 휘저어 식힌 후 퍼담으면 응축수의 생성량이 적었다. 도시락밥통 상하에 단열재를 놓아 보자기에 싸서 보관하면 겨울철에도 차가운 느낌이 없이 도시락밥을 먹을 수 있었다.