• Title/Summary/Keyword: Agitation design

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Effect of Process Parameters on Microhardness of Ni-Al2O3 Composite Coatings (Ni-Al2O3 복합코팅의 마이크로 경도에 대한 공정변수의 영향)

  • Jin, Yeung-Jun;Park, Simon
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.6_2
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    • pp.1037-1045
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    • 2022
  • In this study, nanoscale Al2O3 ceramic particles were used due its exceptionally high hardness characteristics, chemical stability, and wear resistance properties. These nanoparticles will be used to investigate the optimal process conditions for the electro co-deposition of the Ni-Al2O3 composite coatings. A Watts bath electrolytic solution of a controlled composition along with a fixed agitation speed was used for this study. Whereas the current density, the pH value, temperature and concentration of the nano Al2O3 particles of the electrolyte were designated as the manipulative variables. The experimental design method was based on the orthogonal array to find the optimum processing parameters for the electro co-deposition of Ni-Al2O3 composite coatings. The result of confirmation experimental based on the optimal processing condition through the analysis of variance ; EDX analysis found that the ratio of alumina increased to 8.65 wt.% and subsequently the overall hardness increased to 983 Hv. Specially, alumina were evenly distributed on Nickel matrix and particles were embedded more firmly and finely in Nickel matrix.

Optimization of Production Medium by Response Surface Method and Development of Fermentation Condition for Monascus pilosus Culture (Monascus pilosus 배양을 위한 반응표면분석법에 의한 생산배지 최적화 및 발효조건 확립)

  • Yoon, Sang-Jin;Shin, Woo-Shik;Chun, Gie-Taek;Jeong, Yong-Seob
    • KSBB Journal
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    • v.22 no.5
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    • pp.288-296
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    • 2007
  • Monascus pilosus (KCCM 60160) in submerged culture was optimized based on culture medium and fermentation conditions. Monacolin-K (Iovastatin), one of the cholesterol lowing-agent which was produced by Monascus pilosus may maintain a healthy lipid level by inhibiting the biosynthesis of cholesterol. Plackett-Burman design and response surface method were employed to study the culture medium for the desirable monacolin-K production. As a result of experimental designs, optimized production medium components and concentrations (g/L) were determined on soluble starch 96, malt extract 44.5, beef extract 30.23, yeast extract 15, $(NH_4)_2SO_4$ 4.03, $Na_2HPO_4{\cdot}12H_2O$ 0.5, L-Histidine 3.0, $KHSO_4$ 1.0, respectively. Monacolin-K production was improved about 3 times in comparison with shake flask fermentation of the basic production medium. The effect of agitation speed (300, 350, 400 and 450 rpm) on the monacolin-K production were also observed in a batch fermenter. Maximum monacolin-K production with the basic production medium was 68 mg/L when agitation speed was 500 rpm. And it was found that all spherical pellets (average diameter of $1.0{\sim}1.5mm$) were dominant during fermentation. Based on the results, the maximum production of 185 mg/L of monacolin-K with the optimized production medium was obtained at pH (controlled) 6.5, agitation rate 400 rpm, aeration rate 1 vvm, and inoculum size 3%.

20세기말 패션 디자인에 나타난 신표현주의적 이미지에 관한 연구

  • 이효진
    • Journal of the Korean Society of Costume
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    • v.40
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    • pp.5-23
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    • 1998
  • The main purpose of this study was intended to analyze the image of N대-Expressionism represented in the late of 20th century fashion design. By the late 1960s and the early 1970s. the prevailing notion of modernity, which had pushed the limits of art beyond previous boundaries, had begun to lose its urgency. Critics called the new pluralistic era which the West was entering Post-Modern. Furthermore, the predominance of America and the New York scene is diminishing, and artistic leadership is now international. Post-Modernism dialectcally made denial of Modernism as likely as New Image Painting and Decorative Pattern Painting Art in 1970's and it was availed as a dialectcal means for the pre-diction of new comings that would be appeared at painting art in 1980's. New Image Painting has been called as Neo-Expressionism. The N대-Expressionists selected human's feature because appeared flankly, directly irregular agitation in the visual effect and they believed human's destructive and amputate body was cruelty. So they express it on the surface canvas. Under the these background, the image of Neo-Expressionism was represented in the late of 20th century fashion design such as the upside-down image of human feature, the image as ameditation on German myth and history, culture, the ecletic image is made of use a mixture of material. The properties of composition, line, color, texture, and form, common to all plastic art, are now more readily recognized and historically valued in every work. That is, individuality, humanity, and the human condition have been at the core of most Western art and Fashion design. Especially Fashion design has been one of the principal instruments used to examine our nature and to promote the notion of growth, self-understanding, and change.

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Synthesis and characterization of poly(vinyl-alcohol)-poly(β-cyclodextrin) copolymer membranes for aniline extraction

  • Oughlis-Hammache, F.;Skiba, M.;Hallouard, F.;Moulahcene, L.;Kebiche-Senhadji, O.;Benamor, M.;Lahiani-Skiba, M.
    • Membrane and Water Treatment
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    • v.7 no.3
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    • pp.223-240
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    • 2016
  • In this study, poly(vinyl-alcohol) and water insoluble ${\beta}$-cyclodextrin polymer (${\beta}$-CDP) cross-linked with citric acid, have been used as macrocyclic carrier in the preparation of polymer inclusion membranes (PIMs) for aniline (as molecule model) extraction from aqueous media. The obtained membranes were firstly characterized by X-ray diffraction, Fourier transform infrared and water swelling test. The transport of aniline was studied in a two-compartment transport cell under various experimental conditions, such as carrier content in the membranes, stirring rate and initial aniline concentration. The kinetic study was performed and the kinetic parameters were calculated as rate constant (k), permeability coefficient (P) and flux (J). These first results demonstrated the utility of such polymeric membranes for environmental decontamination of toxic organic molecules like aniline. Predictive modeling of transport flux through these materials was then studied using design of experiments; the design chosen was a two level full factorial design $2^k$. An empirical correlation between aniline transport flux and independent variables (Poly ${\beta}$-CD membrane content, agitation speed and initial aniline concentration) was successfully obtained. Statistical analysis showed that initial aniline concentration of the solution was the most important parameter in the study domain. The model revealed the existence of a strong interaction between the Poly ${\beta}$-CD membrane content and the stirring speed of the source solution. The good agreement between the model and the experimental transport data confirms the model's validity.

Optimization of Medium and Fermentation Conditions for Mass Production of Bacillus licheniformis SCD121067 by Statistical Experimental Design (Bacillus licheniformis SCD121067 균체 생산성 증가를 위한 통계적 생산배지 및 발효조건 최적화)

  • Jeong, Yoo-Min;Lee, Ju-Hee;Chung, Hea-Jong;Chun, Gie-Taek;Yun, Soon-Il;Jeong, Yong-Seob
    • KSBB Journal
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    • v.25 no.6
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    • pp.539-546
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    • 2010
  • In this work, mass production of Bacillus licheniformis SCD121067 through medium optimization by statistical experimental method was studied. First, galactose, yeast extract and potassium phosphate dibasic were selected as carbon, nitrogen and phosphate sources for mass production of B. licheniformis SCD121067 by using one factor at a time method. Second, according to the result of Plackett-Burman experimental design, key factors was yeast extract and $K_2HPO$. Finally, the response surface methodology was performed to obtain the optimum concentrations of two selected variables. The optimized medium composition consisted of 20 g/L galactose, 36 g/L yeast extract, 0.41 g/L $K_2HPO4$, 0.25 g/L $Na_2CO_3$, 0.4g/L $MgSO_4$ and 0.01g/L $CaCl_2$. Dry cell weight (15.4 g/L) by optimum production medium were increased 10 times, as compared to that determined with basic production medium (1.5 g/L). Fermentation conditions were examined for the mass production of B. licheniformis. The effect of temperature, agitation speed, pH and aeration rate on the mass production of B. licheniformis were also studied in a batch fermenter which was carried out in a 2.5 L bioreactor with a working volume of 1.5 L containing optimized production medium. As a result, dry cell weight of batch culture was 30.7 g/L at $42^{\circ}C$, 300 rpm, pH 8.0 and 2 vvm.

미생물유래 Transglutaminase의 생산을 위한 생물반응기 운전 조건 확립

  • Lee, Hwa-Jeong;Yu, Jae-Su;Jeon, Gye-Taek;Jeong, Yong-Seop
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.239-242
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    • 2000
  • Experimental studies on the effects of impeller to provide the microbial transglutaminase derived from Streptoverticillium mobaraense have been conducted. The optimal production medium was determined by latin-square design, and the effects of aeration and agitation were observed by using different sizes and shapes of impellers for the poduction of transglutaminase. The effects of pH and temperature were also evaluated for the production of transglutaminase in flasks. As a result, pH is more effective than temperature for both enzyme production and growth of the microorganism. The peak enzyme activity for transglutaminase in fermenter was 0.7 U/mL, but this was still well below the avereage enzyme activity, 1,3 U/mL, obtained in flask runs.

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Optimization for Novel Glucanhydrolase Production of Lipomyces starkeyi KSM 22 by Statistical Design

  • PARK, JUN-SEONG;BYUNG-HOON KIM;JIN-HA LEE;EUN-SEONG SEO;KAB-SU CHO;HYUN-JUNG PARK;HEE-KYOUNG KANG;SUN-KYUN YOO;MYUNG-SUK HA
    • Journal of Microbiology and Biotechnology
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    • v.13 no.6
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    • pp.993-997
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    • 2003
  • Response surface methodology was applied to find the optimum conditions for the production of DXAMase (containing both dextranase and amylase activities) based on the cultivation variables (pH, temperature, and agitation rate). The experimental values from the model equation conceded with predicted values in which the predicted values for dextranase and amylase activities were 2.26 and 3.52 U/ml at pH 4, $28^{\circ}C$, 235 rpm, and the corresponding experimental values were 2.41 and 3.68 U/ml, respectively.

Synthesis Conditions of Thiamine Mononitrate and Pharmaceutical Stability of Thiamine Monosalts (질산치아민의 제조조건과 치아민 모노염류의 제제학적 안정성)

  • Park, Hong-Koo
    • YAKHAK HOEJI
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    • v.41 no.5
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    • pp.595-601
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    • 1997
  • The optimal synthesis condition based on the yield of thiamine mononitrate has been attained according to Box-wilson experimental design. The optimal condition was as follows : Molar concentration ratio of $SB1{\cdot}HCL=0.6839$, quantity of $NH_4NO_3=50.09g$, agitation velocity=51.6rpm, reaction temperature=22.8$^{\circ}C$. The stabilities of three kinds of thiamine monosalts(thiamine monoiodide, -monobromide, -monorhodanate) were investigated in sterile solution, compressed tablet and multivitamin capsule, respectively. Assay data are given to show that thiamine monosalts are not significantly higher than that of thiamine mononitrate in sterile solutions and the addition of an acidic stabilizer is unnecessary. The solutions were clear and free of precipitate and didn't discolored after storage at room temperature and 40$^{\circ}C$ for 6 months. The tablets and capsules were assayed initially and at predetermined intervals during storage at room temperature and 40$^{\circ}C$ for 6 months. Thiamine monorhodanate was found to be more stable than other thiamine monosalts in these compressed tablets containing other vitamin of the B complex and the data indicate that thiamine monosalts was similar to that of thiamine mononitrate in the multivitamine capsules.

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Inner harbour wave agitation using boussinesq wave model

  • Panigrahi, Jitendra K.;Padhy, C.P.;Murty, A.S.N.
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.7 no.1
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    • pp.70-86
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    • 2015
  • Short crested waves play an important role for planning and design of harbours. In this context a numerical simulation is carried out to evaluate wave tranquility inside a real harbour located in east coast of India. The annual offshore wave climate proximity to harbour site is established using Wave Model (WAM) hindcast wave data. The deep water waves are transformed to harbour front using a Near Shore spectral Wave model (NSW). A directional analysis is carried out to determine the probable incident wave directions towards the harbour. Most critical threshold wave height and wave period is chosen for normal operating conditions using exceedence probability analysis. Irregular random waves from various directions are generated confirming to Pierson Moskowitz spectrum at 20m water depth. Wave incident into inner harbor through harbor entrance is performed using Boussinesq Wave model (BW). Wave disturbance experienced inside the harbour and at various berths are analysed. The paper discusses the progresses took place in short wave modeling and it demonstrates application of wave climate for the evaluation of harbor tranquility using various types of wave models.

Basic Analysis for Improvement of Mooring Stability Under Long Wave Impact

  • Ha, Chang-Sik;Moon, Seung-Hyo;Lee, Joong-Woo
    • Journal of Navigation and Port Research
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    • v.41 no.5
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    • pp.329-336
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    • 2017
  • This study suggests a general process of analyzing the mooring and cargo handling limit waves, which is an incident to the new energy port under long wave agitation. To reduce damages of ships and harbor structures due to strong wave responses, it is necessary to predict the change of wave field in the mooring berth to make the proper decision by dock master. The berthing area at a new LNG port in the east coast of Korea in this study is frequently affected by oscillations from waves of 8.5~13s periods in the wintertime. The long period waves give difficulties on port operation by lowering the annual berthing ratio. It needs to find the event waves from the real time offshore wave records, which cause over the mooring limits. For that purpose, the wave records from field measurement and offshore wave buoy were analyzed. From numerical simulation, the response characteristics of long period waves in the berthing area were deduced with or without breakwater expansion plan, analyzing the offshore field wave data collected for two years. Some event wave cases caused over the cargo handling and mooring limits as per the standard Korean port design guideline, and those were used for the decision of port operation by dock master, comparing with the real time offshore wave observations.