• 한국식품영양과학회지
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• 제35권8호
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• pp.979-984
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• 2006

과잉 철은 폐경기 여성 및 핀란드 남성에서의 심혈관계질환 증가와 예방통계학적으로 밀접한 관련이 있다고 보고되고 있다. 허혈성 심장질환, 뇌 심혈관계 질환, 암 및 노화의 원인으로 산화적 스트레스가 자유기 반응을 자극하고 지질 과산화 반응 등을 연쇄적으로 촉진시키는데 철이 위험 인자로 인식되고 있다. 그러나 뇌심혈관계 질환 유발의 중요인자인 혈소판 활성화와 관련하여 철로 인한 산화적 스트레스와 항산화작용의 연구는 부족하다고 한다. 산화적 스트레스에서 철 및 과산화수소의 자유기 형성과 관련하여 토끼 혈액에서 분리한 세정 혈소판을 사용하여 연구하였다. 산화적 스트레스를 통해 혈소판 응집을 유도하고 이에 미치는 영향을 연구한 결과에서 $H_2O_2$ 단독 투여시 혈소판 응집작용은 나타나지 않았다. $FeSO_4$ 단독 투여시 농도 의존적으로 혈소판 응집작용이 증가하여 나타내지만, $H_2O_2$ 존재 하에 $FeSO_4$ 투여시 농도 의존적으로 혈소판 응집작용이 증가되어 나타났다. 혈소판 응집을 유도하는 collagen 최적의 농도$(2\;{\mu}g/mL)$보다 낮은 1/10 농도$(2\;{\mu}g/mL)$)에서 $H_2O_2$ 및 $FeSO_4$의 영향은 농도 의존적으로 혈소판 응집작용이 증가되었다. 철 단독 투여시보다 과산화수소와 함께 투여시 농도 의존적으로 혈소판 활성화가 증대되었고 이러한 혈소판 활성화는 NAD/NADP, catalase, glutathione, mannitol, tiron 등에 의해 농도 의존적으로 억제되었고, NADH/NADPH, SOD, aspirin 등에 의해서는 영향이 없었다. 그러므로, 이러한 NAD(H)/NADP(H) cofactor는 혈소판 응집작용을 일으키는 radical을 직접 억제하기보다 radical 생성에 관련하는 것으로 사료된다. 이상의 결과에서 과잉철은 혈소판 활성화에 직접적으로 관여하고 $H_2O_2$ 존재하에 2가 철을 촉매로 하여 Fenton 반응으로 생성된 OH. 자유기가 혈소판 활성화에 중요한 역할을 한다. 그러나 혈소판에서 자유기가 arachidonic acid 대사의 활성화와 인산화 단백질로 인한 세포내 정보전달에 관한 연구가 더 이루어져야 한다고 사료된다.

• 한국안전학회지
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• 제36권2호
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• pp.49-57
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• 2021

In the case of military supplies, any potential failure and causes of failures must be considered. This study is aimed at examining the failure modes of a rotorcraft landing system to identify the priority items. Failure mode and effects analysis (FMEA) is applied to the rotorcraft landing system. In general, the FMEA is used to evaluate the reliability in engineering fields. Three elements, specifically, the severity, occurrence, and detectability are used to evaluate the failure modes. The risk priority number (RPN) can be obtained by multiplying the scores or the risk levels pertaining to severity, occurrence, and detectability. In this study, different weights of the three elements are considered for the RPN assessment to implement the FMEA. Furthermore, the FMEA is implemented using a fuzzy rule base, similarity aggregation model (SAM), and grey theory model (GTM) to perform a comparative analysis. The same input data are used for all models to enable a fair comparison. The FMEA is applied to military supplies by considering methodological issues. In general, the fuzzy theory is based on a hypothesis regarding the likelihood of the conversion of the crisp value to the fuzzy input. Fuzzy FMEA is the basic method to obtain the fuzzy RPN. The three elements of the FMEA are used as five linguistic terms. The membership functions as triangular fuzzy sets are the simplest models defined by the three elements. In addition, a fuzzy set is described using a membership function mapping the elements to the intervals 0 and 1. The fuzzy rule base is designed to identify the failure modes according to the expert knowledge. The IF-THEN criterion of the fuzzy rule base is formulated to convert a fuzzy input into a fuzzy output. The total number of rules is 125 in the fuzzy rule base. The SAM expresses the judgment corresponding to the individual experiences of the experts performing FMEA as weights. Implementing the SAM is of significance when operating fuzzy sets regarding the expert opinion and can confirm the concurrence of expert opinion. The GTM can perform defuzzification to obtain a crisp value from a fuzzy membership function and determine the priorities by considering the degree of relation and the form of a matrix and weights for the severity, occurrence, and detectability. The proposed models prioritize the failure modes of the rotorcraft landing system. The conventional FMEA and fuzzy rule base can set the same priorities. SAM and GTM can set different priorities with objectivity through weight setting.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권2호
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• pp.99-105
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• 2000

Cell-Cell interaction and the extracellular matrix (ECM) are belisved to play essential roles during in vitro culturing of primary hepatocytes in the control of differentiation and in the maintenance of tissue spcific functions. The objective of this study was to examine the effects of degree of cell-cell contact (DCC) on liver sperific function of rat promary hepatocytes. Hepatocyte aggregates with various with various degrees of cell-cell contantact, I. e., dispersed cell, longish aggregate, rugged aggregate, and smooth spheroid were obtained at 1, 5-6, 15-20, and 36-48 hrs, respectively in suspension cultures grown in spinner flasks embedded in Caalginate bead and collagen gel in order. The may result from mass transfer limitation and shear damage caused by agitation during aggregation. The rugged aggregate showed a higer viability and albumin secretion rate than the dispersed cells or the other aggregates. This result indicates the possible enhancement of a bioartificial liver's (BAL) performance using primary hepatocytes and the reduction in time to prepare a BAL through optimization of the immobilization time.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.29-34
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• 2001

The mutant enzymes of N-carbamyl-D-amino aicd amidohydrolase (N-carbamylase) from Agrobacterium radiobacter NRRL B11291, showing a negligible activity, were selected from the library generated by random mutagenesis. From the sequence analysis, these mutants were found to contain the amino acids substitutions at Cys172, Glu47, and Glu146. Previously, Cys172 was reported to be necessary for the enzyme catalysis. The chemical modification of the N-carbamylase by carboxyl group specific chemical reagent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide(EDC), resulted in a loss of activity. The replacement of glutamic acids with glutamines by site-directed mutagenesis led to aggregation of the enzymes. Mutant enzymes fused with maltose binding protein (MBP) were expressed in soluble form, but were inactive. These results indicate that two glutamic acid residues play an important role in structure and function of the N-carbamylase. Multiple sequence alignment of the related enzymes revealed that Glu47 and Glu146 are rigidly conserved, which suggests that tese residues are crucial for the structure and function of the functionally related C-N hydrolases.

• BMB Reports
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• 제48권2호
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• pp.97-102
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• 2015

Neurons in the developing brain form the cortical plate (CP) in an inside-out manner, in which the late-born neurons are located more superficially than the early-born neurons. Fyn, a member of the Src family kinases, plays an important role in neuronal migration by binding to many substrates. However, the role of the Src-homology 2 (SH2) domain in function of Fyn in neuronal migration remains poorly understood. Here, we demonstrate that the SH2 domain is essential for the action of Fyn in neuronal migration and cortical lamination. A point mutation in the Fyn SH2 domain ($Fyn^{R176A}$) impaired neuronal migration and their final location in the cerebral cortex, by inducing neuronal aggregation and branching. Thus, we provide the first evidence of the Fyn SH2 domain contributing to neuronal migration and neuronal morphogenesis.

• Applied Microscopy
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• 제4권1호
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• pp.25-33
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• 1974

Glycine max Merr., Zea mays L.의 엽육세포와 근단세포에서 cytolysome의 기원과 기능에 관하여 추구하였다. Cytolysomes은 원형질막과 세포질의 소포체에서 유래되는데, 원형질막의 함입으로 다소포체인 것과 환상인 다층막성구조로 분화되는 두가지 기본형이 있고, 또 세포질에서는 무과입성 소포체가 세포질의 일부를 분리시키으로서 세포질분리체로 분화된다. 원형질막과 세포질에서 유래된 이들 구조는 각각 막성구조가 증가되면서 액포내로 유리되고 자식성소화과정을 밟아 퇴화되는것 같다.

• Journal of Communications and Networks
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• 제17권5호
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• pp.491-498
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• 2015

Recognition of radar emitter signals is one of core elements in radar reconnaissance systems. A novel method based on singular value decomposition (SVD) and the main ridge slice of ambiguity function (AF) is presented for attaining a higher correct recognition rate of radar emitter signals in case of low signal-to-noise ratio. This method calculates the AF of the sorted signal and ascertains the main ridge slice envelope. To improve the recognition performance, SVD is employed to eliminate the influence of noise on the main ridge slice envelope. The rotation angle and symmetric Holder coefficients of the main ridge slice envelope are extracted as the elements of the feature vector. And kernel fuzzy c-means clustering is adopted to analyze the feature vector and classify different types of radar signals. Simulation results indicate that the feature vector extracted by the proposed method has satisfactory aggregation within class, separability between classes, and stability. Compared to existing methods, the proposed feature recognition method can achieve a higher correct recognition rate.

• Journal of Communications and Networks
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• 제15권2호
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• pp.164-172
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• 2013

We consider the problem of secure and robust clustering for quantized target tracking in wireless sensor networks (WSN) where the observed system is assumed to evolve according to a probabilistic state space model. We propose a new method for jointly activating the best group of candidate sensors that participate in data aggregation, detecting the malicious sensors and estimating the target position. Firstly, we select the appropriate group in order to balance the energy dissipation and to provide the required data of the target in the WSN. This selection is also based on the transmission power between a sensor node and a cluster head. Secondly, we detect the malicious sensor nodes based on the information relevance of their measurements. Then, we estimate the target position using quantized variational filtering (QVF) algorithm. The selection of the candidate sensors group is based on multi-criteria function, which is computed by using the predicted target position provided by the QVF algorithm, while the malicious sensor nodes detection is based on Kullback-Leibler distance between the current target position distribution and the predicted sensor observation. The performance of the proposed method is validated by simulation results in target tracking for WSN.

• BMB Reports
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• 제48권1호
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• pp.13-18
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• 2015

Alzheimer's disease severely compromises cognitive function. One of the mechanisms to explain the pathology of Alzheimer's disease has been the hypotheses of amyloid-pore/channel formation by complex $A{\beta}$-aggregates. Clinical studies suggested the moderate alcohol consumption can reduces probability developing neurodegenerative pathologies. A recent report explored the ability of ethanol to disrupt the generation of complex $A{\beta}$ in vitro and reduce the toxicity in two cell lines. Molecular dynamics simulations were applied to understand how ethanol blocks the aggregation of amyloid. On the other hand, the in silico modeling showed ethanol effect over the dynamics assembling for complex $A{\beta}$-aggregates mediated by break the hydrosaline bridges between Asp 23 and Lys 28, was are key element for amyloid dimerization. The amyloid pore/ channel hypothesis has been explored only in neuronal models, however recently experiments suggested the frog oocytes such an excellent model to explore the mechanism of the amyloid pore/channel hypothesis. So, the used of frog oocytes to explored the mechanism of amyloid aggregates is new, mainly for amyloid/pore hypothesis. Therefore, this experimental model is a powerful tool to explore the mechanism implicates in the Alzheimer's disease pathology and also suggests a model to prevent the Alzheimer's disease pathology.

• 한국위성정보통신학회논문지
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• 제10권3호
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• pp.53-56
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• 2015

최근 스마트폰과 태블릿 PC 등의 무선 랜(WLAN: Wireless Local Area Networks)을 지원하는 디바이스가 급증하고, 이를 이용한 모바일 서비스가 기하급수적으로 빠르게 보급되었다. 이런 상황에 따라 무선 랜은 더 빠른 속도의 데이터 전송에 대하여 요구하였고, 이를 만족하기 위하여 IEEE 802.11n의 표준이 확정되었다. 특히, IEEE 802.11n의 표준에서 A-MPDU(Aggregation MAC Protocol Data Unit)이라는 핵심적인 기술을 발표하였다. 이는 데이터를 전송할 때 발생하는 오버헤드를 감소하여 전송속도 향상에 도움을 주었다. 본 논문에서는 이 A-MPDU 전송하는 데이터 중 TCP 트래픽을 전송할 때 발생하는 문제점에 대하여 논하고, 해결 방안도 제시한다. A-MPDU 방식으로 TCP 데이터를 전송할 때, 특정 MPDU 데이터가 전송이 실패하는 경우 TCP Duplicate ACK을 발생하여 불필요한 TCP 재전송이 발생하게 된다. 이에 TCP가 갖고 있는 TCP cumulative ACK을 이용하여 불필요하게 발생하는 TCP duplicate ACK 생성을 막고 전송효율을 높이는 방안을 해결책으로 제시한다. 이 해결 방안은 여러 개의 TCP ACK을 집적하여 보내는 대신 시퀀스 번호가 가장 높은 TCP ACK을 하나만 대표하여 보내어 불필요한 오버헤드를 감소할 수 있다. 이 방식을 이용하면 기존 표준에서 제안된 방식에 비해 최대 20% 이상의 전체 처리율 향상을 볼 수 있다.