• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2007년도 학술발표회
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• pp.27-31
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• 2007

This study relates to low and medium molecular weight isoflavone-${\beta}$-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-B-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-B-D-glucan for anti-cancer and immunoenhancing effect. In acordance with one aspect of the present study, it deals with a method of producing isoflavone-${\beta}$-D-glucan, which comprises the followings; 1) culturing and separating mushroom mycelia, 2) producing low-medium molecular weight isoflavone-${\beta}$-D-glucan from high molecular weight one. The cytotoxicity on human cnacer cell line (Caco-2, MCF-7), the expression of Cyclin D, Bcl-2, Bax protein, p21 protein, p53 protein in MCF-7 cells assessed by SDS-PAGE and immunoblotting, and other immuno related factors such as TNF-${\alpha}$ and IL-1B activities were examined. Structural identification of isoflavone-${\beta}$-D-glucan which showed cytotoxicity against cancer cell and immunoenhancing effects was carried by separation with DEAE-cellulose column chromatography, TLC, HPLC, IR, NMR. Clinical test for the cancer patients (n=119) for 6 month was carried out, and immunoenhancing factors (NK. cell number, ratio of T4/T8) were checked. We concluded the identified isoflavone-${\beta}$-D-glucan has immuno enhancing effects and could be useful for cancer chemoprevention.

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.845-851
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• 2006

Spent compost wheat straw is an available by-product from edible mushroom production, which constitutes a potential pollutant and is cost effective for disposal. This study was conducted to determine the nutritive value as ruminant feed of spent wheat straw compost from Agaricus bisporus mushroom production. The compost was provided from a mushroom farm, the casing soil was removed from the whole compost, and then it was sun dried and sampled for chemical analysis. An experiment was conducted, in which four wheat straw-based diets comprising control (I), 10% spent straw (II), 20% spent straw (III) and 30% spent straw (IV) were tested in a cross-over design using 8 sheep. Dry matter intake (DMI) was 74.0, 73.8, 70.2 and 57.1 and organic matter intake (OMI) was 62.7, 63.4, 58.0 and 44.4 g per kg $BW^{0.75}$ for diets I, II, III and IV, respectively, which, were significantly (p<0.05) lower for diet IV. Digestible OMI was respectively 33.1, 32.6, 30.6 and, 20.2 g per kg $BW^{0.75}$ on the four diets which were significantly (p<0.05) different between the treatments. Inclusion of spent compost straw up to 20% of the diet did not affect the digestibility of DM, OM, CF, ADF and NDF, but the diet containing 30% compost straw had statistically (p<0.05) lower digestibilities. Nitrogen balance was also significantly (p<0.05) different between the treatments.

• 한국버섯학회지
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• 제8권1호
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• pp.33-36
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• 2010

볏짚 대체 배지를 개발하기 위해 밀짚 혼용 비율에 따른 볏짚 대체 효과를 구명한 결과는 다음과 같다. 후발효 배지의 화학적 성분을 분석한 결과 밀짚을 20% 혼용한 배지의 질소 함량과 C/N율은 각각 2.2%와 16.7이고, 볏짚 단용 배지는 2.0%, 17.9로 조사되었으며, 볏짚 단용 배지보다 초발이소요일수가 빠르고, 수량도 30% 증수되어 대체 효과를 기대할 수 있었다.

• 한국식품조리과학회지
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• 제25권1호
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• pp.98-105
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• 2009

This study examined the effects of adding four different amounts (0%: control, 4%: M-4, 8%: M-8, 12%: M-12) of button mushroom (Agaricus bisporous) powder on the quality characteristics of cookies. The bulk density and pH of the dough, and firmness, color, spread factor, consumer acceptability of cookies, and total phenol compound content and free radical scavenging activity of mushroom powder and cookies were measured. While the bulk density and pH of the dough as well as spread factor of the cookies significantly decreased, the firmness, L values and total phenol compound contents and free radical scavenging activity of the cookies significantly increased with increasing mushroom powder content(p<.05). The consumer acceptability scores for the button mushroom cookie groups ranked significantly(p<.05) higher than those of the control group in general acceptability, appearance, flavor, color, taste, and texture. This study suggests that button mushroom powder is a good ingredient for increasing the acceptability and functionality of cookies.

• Food Quality and Culture
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• 제3권1호
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• pp.6-10
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• 2009

This study examined the effects of adding Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes on the quality characteristics of cookies and multiple purposes of wheat powders. The water content, crude protein content, mineral content, color value and sensory evaluation of mushroom cookies and multiple purposes of wheat powders were measured. In the case of cookies, the water content was 1.10-1.24% and crude protein content was 8.20-9.80%. General preferences, such as appearance, color, taste and texture, increased when mushroom was added to the cookies, especially flavor, which showed a much higher preference. In the studies where mushroom was added to wheat powders, the water content was 0.57-0.92% and crude protein content was 11.90-12.80%. The addition of mushroom to both of the cookies and multiple purposes of wheat powders resulted in a remarkable increase in the mineral content, especially Fe, K. In addition, an increase in the amount of added mushrooms resulted in an increase in the hunter L and a values; however, this also resulted in a decrease in the b value.

• 한국균학회지
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• 제7권2호
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• pp.75-82
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• 1979

1978년(年) 여름부터 가을까지 소백산(小白山) 및 영주지방(榮州地方)에서 탐집(探集)된 고등균류(高等菌類)와 1976년(年) 안동(安東)에서 탐집(探集)된 것중 미동정(未同定)이 었든 것을 이번에 동정(同定)하였다. 그 결과(結果) Macrocystidia의 한국(韓國) 미기록속(未記錄屬)과 10개(個)의 미기록종(未記錄種)을 다음과 같이 확인(確認)하였다. Hygrocybe turunda (Fr.) Karst., Hygrophorus lucorum Kalchbr., Macrocystidia cucumsi (Fr.) Heim var. latifolia (Lange) Imazeki et Hongo, Agaricus subrufescens Peck, Psathyrella hydrophila(Bull. ex Fr.) A.H. Smith, Stropharia rugosannulata Farlow f. lutea Hongo, Inocybe bresadolae Mass., I. multicoronata A.H. Smith, Russula farinipes Romell 과 Lactarius sakamotoi Imai.

• Mycobiology
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• 제36권1호
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• pp.50-54
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• 2008

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by $^{13}C-NMR$. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By $^{13}C-NMR$ analysis, the immuno-stimulating substance was identified as ${\beta}-(1{\rightarrow}3)\;(1{\rightarrow}6)$-glucan, composed of a backbone with $(1{\rightarrow}3)$-linked D-glucopyranosyl residues branching a $(1{\rightarrow}6)$-linked D-glucopyranosyl residue. The ${\beta}$-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

• 생명과학회지
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• 제8권1호
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• pp.27-31
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• 1998

Isoprenoid quinones은 미생물의 호흡계나 관합성의 전자전달계의 필수적인 구성성분으로, 그들의 화학적분류 뿐만 아니라 생리학적인 중요성으니 충분히 인식되어 있다. 본실험에서는 Trichoderma, Gliocladium, Verticillium, Aspergullus 및 Agaridus bisporus, 및 Agaricus biporus, Lenrinus edodes, Pleurotus ostrearus igniarius, Phellinus chrysolona를 포함한 일부 식용버섯의 ubiquinone type을 결정하였다. 대부분의 불완전균휴는 Q-10($H_2$)를, 본 실험에 사용된 식용버섯 전체는 Q-9을 main ubipuinone type을 가지는 것으로 나타났다. 따라서 upiquinone type은 진균류에 있어서도 화학적 분류의 유용한 수단으로 간주되며, 보다 광범위한 연구결과의 축척이 필요한 것이다.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1331-1337
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• 2006

A mathematical model has been proposed for the batch culture of Agaricus blazei with mixed carbon sources of glucose and dextrin. In the proposed model, the metabolism of A. blazei was divided into three parts: cell growth, exopolysaccharides (EPS) production, and another EPS production pathway activated by dextrin hydrolysis. Each pathway was described mathematically and incorporated into the mechanistic model structure. Batch cultures were carried out with six different carbon source compositions. Although parameters were estimated by using the experimental data from the two extreme cases such as glucose only and dextrin only, the model represented well the profiles of glucose, cell mass, and EPS concentrations for all the six different carbon source mixtures, showing a good interpolation capability. Of note, the lag in EPS production could be quite precisely predicted by assuming that the glucose-to-cell mass ratio was the governing factor for EPS production.

• 한국균학회지
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• 제46권2호
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• pp.105-113
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• 2018

본 연구에서는 2016년 5월에서 9월까지 제주 동부지역에서 채집된 317종의 버섯들 중 국내 미기록된 4종을 보고하고자 한다. 미기록종으로 추정되는 버섯의 정확한 동정을 위하여 채집된 자실체에서 분리한 DNA의 염기서열을 버섯의 유전자은행을 통하여 비교 분석한 결과 Agaricus flocculosipes, Amanita concentrica, Coprinopsis strossmayeri, Favolus acervatus로 동정되었으며 이들은 모두 국내 미기록종으로 확인되었다. 한편 현미경을 이용하여 버섯의 미세구조를 관찰한 결과는 모두 DNA 염기서열을 분석하여 동정한 버섯과 일치하였다.