• Proceedings of the Plant Resources Society of Korea Conference
• /
• 1998.10a
• /
• pp.48-50
• /
• 1998

• Journal of Applied Biological Chemistry
• /
• v.50 no.1
• /
• pp.1-5
• /
• 2007

Chemical properties of spray-dried powders separated based on molecular weight from crude protein binding polysaccharide (CP-SD) of Agraricus blazei were examined. Contents of ${\beta}$-glucan in SD-1, SD-2 and SD-3 were 18.67%, 48.24%, and 37.15% respectively, and SD-2 (10-150 kDa) showed the highest molecular weight. Obtained ${\beta}$-glucans were not pure glucan, but was determined to be an acidic proteo-heteroglycan with a large amount of glucose (74.46-80.05%), galactose (8.91-15.2%), and mannose (4.9-5.46%). Composition of their amino acids was mainly aspartic and glutamic acids. FT-IR spectrum revealed SD-1, SD-2 and SD-3 were structures of ${\beta}$-1,3-glucans and ${\alpha}$-1,6-glucans at 890 and 930 $cm^{-1}$, respectively, signals of ${\alpha}$-1,6-glucans for CP-SD was not found. Useful CP-SD was recovered from A. blazei for preparation of three powder types as food materials.

• Journal of Mushroom
• /
• v.9 no.4
• /
• pp.198-201
• /
• 2011

Cobweb disease symptoms were observed in a mushroom farm in Buye, Korea during a disease survey in 2008-2011. Five isolates of Cladobotryum sp. were obtained from the infected caps and stipes. These isolates of Cladobotryum sp. were identified as C. mycophilum based on their morphological, cultural characteristics and analysis of the ITS sequences. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. Optimal temperature and pH for mycelial growth on MEA is $23^{\circ}C$ and 6.0. Microscopically the spores of the fungus are large and most 2~3 celled produced on vertically branched conidiophores. Mushroom caps turned dark brown and shrunk due to soft rot. Testing of sensitivity to selected fungicides showed that isolate was highly resistance to Mancozeb and Thiophanate-methyl, moderately sensitivity to Iprodione, and highly sensitivity to Benomyl, Prochloraz-Mn and Carbendazim.

• Food Science and Preservation
• /
• v.2 no.2
• /
• pp.225-232
• /
• 1995

In order to study the effect of modified atmosphere packaging(M.A.P.) and controlled atmosphere(C. A.) storage on keeping freshness of mushrooms (Agricus-bisporus). Mushrooms was packaged with polyethylene(P.E.) film (40${\mu}{\textrm}{m}$, 60${\mu}{\textrm}{m}$, 80${\mu}{\textrm}{m}$) and C.A. conditions(CO2 concentration of 2%, O2 concentration of 2%) and storage at 0$\pm$1$^{\circ}C$, RH(relative hummidity) 92$\pm$1%. Gas composition in film was changed rapidly at early of storage, but it kept a constant level after 14 days of storage, and then kept at the level of 6~9% CO2 and 2~5% O2. Weight loss was 5% in non-packed mushrooms after 3 days of storage, but P.E. film, CO2 treated, C.A. conditions were 5% after 21 days of storage. Flesh firmness of the mushrooms was continuously decreased throughout storage period and the lowest of flesh firmness changed was 80${\mu}{\textrm}{m}$-P.E. film packing. Discoloration of the piteous of mushrooms appears to be the most Important factors to determine its marketability, L value of it appears to be of the high values at P.E. film packing and C.A. conditions during period. Large amount of ethanol and acetaldehyde were produced from the 7 days during storage, large contents of mannitol and trehalose were at the 14 days and 7 days during storage.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.16 no.4
• /
• pp.333-336
• /
• 1987

The Aroma components analysis of raw and boiled mushroom (Agaricus bisporus) by GC, GC-MS is as follows; 1) The volatile aroma component of raw mushroom was identified as 13 Kinds, ana among them, 4 Kinds of aroma component such as 1-octen-3-ol (73.13%), 2-octenol (7.44%), 3-octanone (3.09%), 3-octanol (1.42%)-consists 85.08% of total aroma component. 2) Meanwhile, the volatile aroma component of toiled one was indentified as 13 Kinds and 5 of them 1-octen-3-ol (68.86%), 2-octenol (8.42%), 3-octanone (3.15%), Benzyl alcohol (3.23%), 3-octanol (1.97%)-consists 86.23% of total aroma component.

• Journal of Mushroom
• /
• v.12 no.4
• /
• pp.237-243
• /
• 2014

Spawn is the vegetative growth of the mycelium and serves as the inoculum or seed for cultivating mushrooms. Various cereal grains, such as wheat, millet, barley, sorghum, brown rice, rye, and oat were assessed to compare their characteristics and suitability for spawn production of button mushroom (Agaricus bisporus). The mycelial growth rates, density, and the number of completely colonized grains were measured from the twentieth day of inoculation. Wheat grains showed fastest mycelial growth with 8.4 cm followed by rye, oat, barley with 8.2, 7.5 and 7.3 cm, respectively. In the mycelial density, foxtail millet, barley, and sorghum were best compared with that of wheat grains. Especially, the number of grains which were completely colonized by mycelia were greatest in foxtail millet with 5,123 grains followed by proso millet, and wheat with 3,052 and 914, respectively. Based on the results obtained, barley, foxtail millet, and sorghum grains would be appropriate substituting for wheat grain in spawn production of button mushroom.

• Journal of environmental and Sanitary engineering
• /
• v.2 no.3 s.3
• /
• pp.7-11
• /
• 1987

A Comparison on the fatty acid composition of 7 species of Korean edible mushroom(Agaricus bisporus, Tricholoma matsutake, Lentinus edodes, Pleurotus ostreatus, Ramaria botrytis, Sarcodon asparatus, Calocybe gambosa) were investigated by gasliquid Chromatography. The results were obtained as follows: 1) The major fatty acid in all samples were linoleic $(77.33-35.53\%)$, oleic $(39.69-1.58\%)$ and palmitic$(22.51-7.31\%)$ acid, 31 2) The content of linoleic acid was the highest in Agaricus Bisporus, Pleurotus ostreatus and Lentinus edodes meanwhile, the content of oleic acid of these mushrooms was significantly low compared with others. 3) Quantity of myristic acid was higher in comparison with fatty acid composition from common lipid source.

• Environmental Mutagens and Carcinogens
• /
• v.19 no.2
• /
• pp.112-115
• /
• 1999

The action mechanism of the inhibitory effect of some natural products on the DNA strand break and DNA damage was investigated in vitro and in vivo. In the E. coli chromosomal DNA strand break experiment in vitro, three mushroom water extracts were effective on the DNA strand breaking by daunorubicin. Phellinus linteus water extract inactivated daunorubicin, a DNA strand breaking agent, but did not protect DNA from daunorubicin-induced DNA strand breaking. Agaricus blazei water extract inhibited DNA strand breaking action of daunorubicin not only by daunorubicin inactivation, but also by DNA protection from daunorubicin. An inhibitory effect of Ganoderma lucidum water extract on the DNA strand break was based on the DNA protection rather than daunorubicin inactivation. In vivo mutagen assay system (SOS-chromotest), among three mushroom water extracts Phellinus linteus water extract was the most effective one on the inhibition of DNA damage by 4-NQO. The results suggest that all three mushroom water extracts inhibit daunorubicin-induced DNA damage and in vivo DNA damaging action of 4-NQO by the reaction of mutagen inactivation or DNA protection from the mutagen.

• Journal of Environmental Health Sciences
• /
• v.21 no.4
• /
• pp.87-95
• /
• 1995

The concentrations of cadmium, metallothionein(MT), superoxide dismutase(SOD), and lactate dehydrogenase(LDH) were investigated in liver and kidney of rats which were fed the water containing 100 ppm cadmium chloride with basal diet and 5% Agaricus bisporus diet during 16 weeks. Cadmium concentrations in liver and kidney increased during 16 weeks, and there were significantly higher accumulation of cadmium in the kidney than in the liver. The concentrations of MTs in liver and kidney decreased linearly during 16 weeks, but there was no significant difference between control and experimental group. MT concentrations of liver were significantly higher than those of kidney. The superoxide dismutase activities and lactate dehydrogenase activities were not affected by the diet, but there was a significant difference by the duration of administration. These data indicate that the kidney is a major target organ of chronic cadmium poisoning, and suggest that Cd-induced hepatic injury, via release of Cd-MT, may play an important role in the nephrotoxicity. In conclusion, induction of MT occurs in both the liver and the kidney after administration of $CdCl_2$. However, the kidney is less responsive than the liver to the induction of MT by cadmium, which may contribute to making the kidney the target organ of toxicity during chronic Cd exposure.

• Korean journal of applied entomology
• /
• v.10 no.1
• /
• pp.59-62
• /
• 1971

The highest fresh weight of Agaricus bisporus was obtained at pH 7.5 of casing soil. Above or below this pH level, the yields were significantly reduced. Stimulatory effect of calcium on the mycelial growth depended upon pH of casing soil, In neutral or slightly alkaline soil, the stimulatory effect was increased. The effects of supplementing calcium carbonate or hydrated lime to casing soil were assumed not only the influence of pH of casing soil, but also stimulatory effect of calcium ion on mycelial growth of A. bisporus.