• Journal of Microbiology
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• 제34권2호
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• pp.117-123
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• 1996

Restriction-polymorphic patterns of nuclear-ITS were examined for the genetic relationships among 12 bisidiomycetous mushrooms to Aphyllophorales and Agaricales. The taxonomic affinity of Ganoderma species with the family Polyporaceae also was examined. With 13 restriction endonucleases, 159 restriction characters were generated form the 12 species examined. UPGMA and neighbor-joining analyses separated the 12 species into two genetically distinct groups that correspond to orders (Agaricales and Aphyllophorales) where each species is included. This result indicates that there is clear genetic demarcation between Agaricales and Aphyllophorales. Dendrograms constructed by several data analyses showed that even though Ganoderma species are somewhat in intermediate taxonomic position between the Polyporaceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

• Mycobiology
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• 제50권4호
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• pp.219-230
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• 2022

Agaricales species form pileate-stipitate fruiting bodies and play important roles in maintaining the terrestrial ecosystem as decomposers, symbionts, and pathogens. Approximately 23,000 Agaricales species have been known worldwide, and 937 species have been recorded in the Republic of Korea. However, most of them were identified solely based on morphological characteristics that often led to misidentifications. The specimens collected from 2018 to 2020 in the Republic of Korea were identified based on phylogenetic analysis of the internal transcribed spacer (ITS) sequences. Their identities were confirmed by microscopic characteristics. As a result, 14 Agaricales species were discovered for the first time in the Republic of Korea. They belonged to nine genera: Agaricus, Calocybe, Cortinarius, Hygrocybe, Inocybe, Lepista, Leucoagaricus, Marasmius, and Psathyrella. Detailed macroscopic and microscopic descriptions were provided to help distinguish these species. The morphological and molecular data provided in this study will serve as reliable references for the identification of Agaricales species.

• 한국균학회지
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• 제47권4호
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• pp.281-289
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• 2019

Since 2013, more than 100 Entoloma (Entolomataceae, Agaricales) collections have been made during field investigations of mushroom flora in Korea. Among these collections, three Entoloma species were identified as new records from Korea. Morphological examinations were made based on the macro- and micro-morphological features of the isolates collected during field visits. To ensure the identity of the isolates at the species level, DNA sequences from four gene regions (rpb2, ITS, 28S, and mtSSU) were compared. To the best of our knowledge, these are the first records of E. aprile, E. chytrophilum, and E. hirtipes in Korea. Comprehensive descriptions, photographs, and phylogenetic examinations are presented here.

• Current Research on Agriculture and Life Sciences
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• 제22권
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• pp.29-39
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• 2004

This study was conducted to provide a fundamental information for commercial, medical usage and mushroom gene preservation and to investigate the diversity of indigenous mushroom on Bonghwa-Gun. A total of 3 classes 2 subclasses 13 orders 45 families 64 genera and 118 species including saprophytic and ectomycorrhizal fungi was investigated. The mushroooms are classified into 17 families 32 genera and 80 species in Agaricales, 13 families 17 genera and 22 species in Aphllophorales, 2 families 2 genera and 2 species in Heterobasidiomycetes, 9 families 9 genera and 10 species in Gasteromycetidae. There were edible mushroom of 46 species at Moonsu and Chungryang Mountain. The mushroom occurrence was closely related to vegetation and soil environmental conditions.

• 한국균학회지
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• 제49권2호
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• pp.139-153
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• 2021

The Inocybaceae is a family of ectomycorrhizal fungi belonging to the Agaricales order. Most species in this family produce secondary metabolites, such as muscarine, psilocybin, and other compounds. In the present study, we conducted taxonomic studies on the Inocybaceae based on morphological and molecular phylogenetic analyses for the first time in Korea. As a result, seven species of Inocybaceae were newly recorded: Inocybe caroticolor, I. fuscidula, I. grammatoides, I. latibulosa, I. stellata, Inosperma shawarense, and Mallocybe malenconii. Among them, the genera Inosperma and Mallocybe were described for the first time in Korea. Relevant morphological characteristics, phylogenetic trees, and photographs are provided.

• Mycobiology
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• 제48권4호
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• pp.288-295
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• 2020

Species of Laccaria (Hydnangiaceae, Agaricales, and Basidiomycota) are well-known ectomycorrhizal symbionts of a broad range of hosts. Laccaria species are characterized by brown, orange, or purple colored basidiocarps, and globose or oblong, echinulate and multinucleate basidiospores. While some Laccaria species are easily identified at the species level using only the morphological characteristics, others are hard to distinguish at the species level due to small differences in morphology. Heretofore, ten Laccaria species have been reported in Korea. While studying the fungal diversity in the National Parks of Korea, two new Laccaria species were discovered. Species identification was done based on molecular analyses (ITS, 28S rDNA, rpb2, and tef1), then were confirmed by their corresponding morphologies. The two newly discovered Laccaria species are proposed here as Laccaria macrobasidia and Laccaria griseolilacina. The unique morphological characters of L. macrobasidia that distinguish it from its closely related species are orange-brown colored basidiocarp, long basidia and the absence of cheilocystidia. L. griseolilacina is characterized by a light grayish lavender-colored pileus and the absence of cheilocystidia. Two new species are described and illustrated in the present paper.

• Mycobiology
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• 제48권1호
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• pp.58-69
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• 2020

Meghalaya, (in India), in the region of the mega-biodiversity hotspots, is home to a plethora of wild mushrooms. The present study concerns the exploration of the order Agaricales, which includes rare gilled mushrooms considered endangered under IUCN A4c criteria, due to the declining habitat. Electron microscopy of the gill sections revealed an abundance of clamp connections, hyphal cell walls, cystidia, and basidia. This rare species which belongs to the family Cyphellaceae, exhibits morphological and molecular differences from the Cyphella spp. Phylogenetic analysis revealed that it formed a clade under the genus Campanophyllum of the order Agaricales, confirmed by both Neighbor Joining (NJ) and Bayesian phylogenetic analysis. Being nutritionally potent along with its efficient antioxidant value, the fungal extract shows significant rise of two-fold in the antimicrobial activity along with the commercial antibiotics. The compound, Phenol, 2, 4-bis (1, 1-Dimethylethyl) (2, 4-DTBP) showed in ample range in the fungal extract along with aliphatic hydrocarbons, terpene, alcohol and volatile organic compounds on further characterization in GCMS. The present study indicates the endangered Campanophyllum proboscideum could be a rich source of natural antioxidants and an effective pharmaceutical agent.

• Mycobiology
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• 제43권3호
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• pp.239-257
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• 2015

We conducted five times surveys, in June, September and October in 2012; June and September 2013, to catalog the mushroom flora in Ulleung-gun, Republic of Korea. More than 400 specimens were collected, and 317 of the specimens were successfully sequenced using the ribosomal DNA internal transcribed spacer barcode marker. We also surveyed the morphological characteristics of the sequenced specimens. The specimens were classified into 2 phyla, 7 classes, 21 orders, 59 families, 122 genera, and 221 species, and were deposited in the herbarium of Korea National Arboretum. Among the collected species, 72% were saprophytic, 25% were symbiotic, and 3% were parasitic. The most common order was Agaricales (189 specimens, 132 species), followed by Polyporales (47 specimens, 27 species), Russulales (31 specimens, 22 species), Boletales (10 specimens, 7 species), and so on. Herein, we also reported the first Bovista species in Korea, which was collected from Dokdo, the far-eastern island of Korea.

• 한국균학회지
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• 제38권2호
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• pp.192-196
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• 2010

본 연구는 한국산 주름버섯목 중에서 아직 미발표된 2속과 2종에 대해 상세하게 기술하고 미세구조를 도해하였다. 저자들은 한국 내 미기록속인 까마귀버섯속과 노을버섯속에 대한 속 특징기술과 미기록종인 까마귀버섯과 살구노을버섯에 대한 종 특징기술을 하였으며 한국명을 신칭하여 보고하고자 한다. 본 연구에 사용된 표본은 국립농업과학원 식물균류표본보존센타(HCCN)에 보존되어 있다.

• 한국균학회지
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• 제44권4호
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• pp.220-232
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• 2016

2009년부터 2011년, 2015년까지 변산반도 국립공원의 외생균근성 버섯을 조사한 결과, 총 1문 1강 5목 13과 33속 131종 3,624개체가 조사되었으며, 주름버섯목(Agaricales)이 6과 9속 49종 1,343개체, 무당버섯목(Russulales) 1과 2속 35종 854개체, 그물버섯목(Boletales) 4과 19속 40종 805개체, 꾀꼬리버섯목(Cantharellales) 1과 2속 5종 609개체 및 사마귀버섯목(Thelephorales) 1과 1속 2종 13개체의 순으로 발생되어 대부분의 외생균근성 버섯은 주름버섯목, 무당버섯목 및 그물버섯목 등 3목에 속하는 것으로 나타났다. 가장 많이 조사된 외생균근성 버섯은 무당버섯과로 35종이었으며, 그물버섯과(34종) 및 광대버섯과(25종)순으로 나타났다. 개체수는 무당버섯과가 854개체로 가장 많았고 광대버섯과(754개체) 및 그물버섯과(652개체) 순으로 조사되었다. 월별 발생에서는 대부분의 외생균근성 버섯 및 우점 외생균근성 버섯의 종수 및 개체수는 7월에 집중하는 것으로 나타났다. 고도별에서는 100 m 이하에서 가장 많은 종수 및 개체수가 조사되었고 300 m 이상에서는 현저히 적어지는 것으로 나타났다. 대부분의 외생균근성 버섯 및 우점 외생균근성 버섯은 평균온도 $23.0{\sim}25.9^{\circ}C$, 최고온도 $28.0{\sim}29.9^{\circ}C$, 최저온도 $21.0{\sim}22.9^{\circ}C$, 상대습도 77.0~79.9%, 강수량 300.0 mm 이상인 시기에 다양한 종 및 개체수 발생이 높은 것으로 나타났다.