• 대한약침학회지
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• 제27권1호
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• pp.1-13
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• 2024

Background: Diabetic nephropathy (DN) is one of the major complications of chronic hyperglycaemia affecting normal kidney functioning. The ayurvedic medicine curcumin (CUR) is pharmaceutically accepted for its vast biological effects. Objectives: The Curcuma-derived diferuloylmethane compound CUR, loaded on Poly (lactide-co-glycolic) acid (PLGA) nanoparticles was utilized to combat DN-induced renal apoptosis by selectively targeting and modulating Bcl2. Methods: Upon in silico molecular docking and screening study CUR was selected as the core phytocompound for nanoparticle formulation. PLGA-nano-encapsulated-curcumin (NCUR) were synthesized following standard solvent displacement method. The NCUR were characterized for shape, size and other physico-chemical properties by Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS) and Fourier-Transform Infrared (FTIR) Spectroscopy studies. For in vivo validation of nephro-protective effects, Mus musculus were pre-treated with CUR at a dose of 50 mg/kg b.w. and NCUR at a dose of 25 mg/kg b.w. (dose 1), 12.5 mg/kg b.w (dose 2) followed by alloxan administration (100 mg/kg b.w) and serum glucose levels, histopathology and immunofluorescence study were conducted. Results: The in silico study revealed a strong affinity of CUR towards Bcl2 (dock score -10.94 Kcal/mol). The synthesized NCUR were of even shape, devoid of cracks and holes with mean size of ~80 nm having -7.53 mV zeta potential. Dose 1 efficiently improved serum glucose levels, tissue-specific expression of Bcl2 and reduced glomerular space and glomerular sclerosis in comparison to hyperglycaemic group. Conclusion: This study essentially validates the potential of NCUR to inhibit DN by reducing blood glucose level and mitigating glomerular apoptosis by selectively promoting Bcl2 protein expression in kidney tissue.

• 한국작물학회지
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• 제65권2호
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• pp.79-83
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• 2020

본 연구에서 남부 평야지 조기재배 조건에서 생산된 쌀을 색채선별기를 이용한 쌀의 외관특성 향상과 밥맛의 향상을 위해 품질관련 특성을 분석하였다. 해담쌀 백미를 색채선별기에 2회 통과하여 등급별로 분류한 결과, A급은 64.8%, B급 18.0%, C급 17.2%이였다. 분류등급별 쌀을 백미완전미율을 분석한 결과, A급은 72.9%로 색채선별전 쌀 48.4%보다 약 25.4%정도 향상되었다. B등급과 C등급은 원료곡보다 완전미율이 낮았다. 쌀의 백도는 A등급이 45.3으로 색채선별전의 원곡보다 1.7정도 낮아졌다. 색상색차계 적색도 a값은 색채선별전 대비구에 비해 유의하게 낮았다. 아밀로스 함량과 단백질 함량은 색채선별 전후 유의한 차이가 없었으나, 쌀의 호화특성은 정상립으로 분류된 A등급은 최고점도와 최종점도가 각각 266.1RVU와 215.9 RVU로 색채선별전보다 높았고, 밥의 윤기치에서도 68.8로 색채 선별전보다 증가하였다. 이 결과로 볼 때 평야지 조기재배 조건에서 생산된 쌀을 색채선별기를 이용한 쌀의 외관특성을 분류함으로써 완전미율과 밥맛이 향상될 수 있을 것으로 기대된다.