• Research in Plant Disease
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• v.12 no.3
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• pp.235-239
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• 2006

Since the cultivation of resistance cultivar is essential for organic agriculture, Phytophthora late blight resistance of 16 advanced potato clones obtained from the potato breeding laboratory at the National Institute of Highland Agriculture was evaluated. Resistance of the clone was examined through artificial inoculation in the laboratory and in the field. Dominant isolates of the pathogen in Gangwon province produced abundant sporangia on leaves of most clones showing susceptibility. The number of sporangia ranged from $10^{7{\sim}8}sporangia/ml$ in the susceptible clones at 7 days after inoculation. However, one clone is resistant in organic farming fields. Disease incidence was 2.3% using the resistant clone. The area under disease progress curve(AUDPC) was 75.5. Contrarily, disease incidence of the susceptible variety was 100% and the AUDPC was 1773.5 during the same cultivation period. The resistance clone named as 'Haryeong' is considered suitable for organic potato cultivation in Gangwon province in Korea.

• Environmental Engineering Research
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• v.20 no.4
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• pp.377-382
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• 2015

The high concentration of nitrogen and phosphorus in wastewater incorporated with the ability to use carbon dioxide as the carbon source make the microalgae become more attractive in wastewater treatment process. This study evaluates the optimal conditions for the digestion of settelable solids from the recirculating aquaculture system to produce the biomass of the green microalga Scenedesmus sp. After solids separation, aerobic digestion of settleable solids under disperse condition produced nitrate as the final product of consequently ammonification and nitrification processes. With the optimal digestion procedure, nitrate concentration during aerobic digestion in 2000 mL vessel increased from $9.63{\pm}0.65mg\;N/L$ to $58.66{\pm}0.06mg\;N/L$ in 10 days. Thereafter, cultivation of Scenedesmus sp. was performed in 1000 mL Duran bottle with air bubbling. The highest Scenedesmus sp. specific growth rate of $0.321{\pm}0.01/d$ was obtained in treatment using liquid fraction after aerobic digestion as the whole culture medium for Scenedesmus sp. cultivation. With this study, digestion of $8,800{\pm}128.12mg\;dry\;weight/L$ of settleable solids from fish pond finally produced $1,235{\pm}21mg\;dry\;weight/L$ of Scenedesmus sp. biomass.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.1
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• pp.6-10
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• 2001

The production of chitosan from the mycelia of Absidia coerulea was studied to improve cell growth and chitosan productivity. Culture conditions were optimized in batch cultivation (pH 4.5, agitator speed of 250 rpm, and aeration rate of 2 vvm) and the maximum chitosan concentration achieved was 2.3g/L under optimized conditions. Continuous culture was carried out successfully by the formation of new growth spots under optimized conditions, with a chitosan productivity of 0.052g/L(sup)-1 h(sup)-1, which is the highest value to date, and was obtained at a dulution rate of 0.05h(sup)-1. Cell chitosan concentrations reached about 14% in the steady state, which is similar to that achieved in batch culture. This study shows that for the continuous culture of Absidia coerulea it is vital to control the medium composition.

• International journal of advanced smart convergence
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• v.12 no.2
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• pp.153-158
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• 2023

Most agriculture plantings are based on traditional farming and demand a lot of human work processes. In order to improve the efficiency as well as the productivity of their farms, modern agricultural technology was proven to be better than traditional practices. Internet of Things (IoT) is usually related in modern agriculture which provides the farmer with a real-time monitoring condition of their farm from anywhere and anytime. Therefore, the application of IoT with a sensor to measure and monitors the humidity and the temperature in the mushroom farm that can overcome this problem. This paper proposes an IoT based monitoring system forindoor Ganoderma lucidum cultivation at a minimal cost in terms of hardware resources and practicality. The results show that the data of temperature and humidity are changing depending on the weather and the preliminary experimental results demonstrated that all parameters of the system were optimized and successful to achieve the objective. In addition, the analysis results show that the quality of Ganoderma lucidum produced on the research method conforms to regulations in Vietnam.

• Journal of Advanced Navigation Technology
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• v.12 no.4
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• pp.324-331
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• 2008

In this paper, we developed the crops monitoring system using wireless sensor networks. It can acquire the information about the cultivation environments from a temperature, humidity, illumination and soil sensor then use the detailed information for very useful. The acquisition about the cultivation environment information with wireless sensor networks can be made up an environment that an administrator is able to confirm the information and wherever in a remote place instead of conventional way visiting the cultivate place by investing a lot of money and time. Therefore, it could be saving money, time and effort, and reduced the damages from the natural disasters such as a cold-weather damage and drought. In addition, we could be possible preventing from the damages of blight and harmful insects according to the temperature by an analysis. In agriculture field, this system could be supported for making a foundation of the advanced technology developing endless, and, in IT field, it is look forward to preparing the opportunity which creates a new requirement on the new technology.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.209-212
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• 1985

The cell density and packing characteristics of Escherichia coli immobilized in a dual hollow fiber bioreactor consisting of outer silicone membrane for oxygen transport and three inner isotropic polypropylene hollow fibers for substrate transport were investigated. The cells have grown forming the layer like animal tissue in a nearly 100％ packing density. The dry biomass density was 550g/liter of void volume for cell growth, which was the highest among the biomass densities ever reported.

• The Korean Journal of Applied Statistics
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• v.25 no.5
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• pp.733-741
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• 2012

The successful launch of Arirang satellites allow the acquisition of high resolution satellite imagery of Korean territory and enables the transition from the conventional cultivation area survey method to new image based methods adopted in advanced nations. In this study, we suggested reasonable sizes of the primary sampling unit and the secondary sampling unit for the satellite imagery based sampling design in 8 provinces preselected for this research. The PSU size was determined mainly in consideration of intracorrelation that shows the degree of homogeneity within each cluster and the efficiency of the image process. For the SSU size, we considered the relative standard error and the differences between the land cover maps produced by the Ministry of Environment and the satellite imagery processed by the National Statistical Office.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.1
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• pp.47-51
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• 2004

Ceramide III was prepared by the cultivation of Saccharomyces cerevisiae. Ceramide III was partitioned from the cell extracts by solvent extraction and analyzed by Normal Phase High Performance Liquid Chromatography (NP-HPLC) using Evaporative Light Scattering Detector (ELSD). We experimentally determined the mobile phase composition to separate ceramide III with NP-HPLC. Three binary mobile phases of n-hexane/ethanol, n-hexane/lsoprophyl Alcohol(IPA) and n-hexane/n-butanol and one ternary mobile phase of n-hexane/IPA/methanol were demonstrated. For the binary mobile phase of n-hexane/ethanol, the first mobile phase composition, 95/5(v/v), was step-increased to 72/23(v/v) at 3 min. In the binary mobile phase, the retention time of ceramide III was 7.87min, while it was 4.11 min respectively in the ternary system, where the mobile phase composition of n-hexane/IPA/methanol, 85/7/8(v/v/v), was step-increased to 75/10/15(v/v/v) at 3 min. However, in the ternary mobile phase, the more peak area of ceramide III was observed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.171-171
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• 2022

Effect of temperature during the period of 10 days from transplanting (10 DFT) on the growth and development of rice plants was investigated by transplanting semi-adult seedlings six times from 5 April to 15 May at 10-day interval in the field conditions of 2020 and 2021, with aims to investigate the critical temperature for early transplanting of temperate rice. In the two experimental years, mean temperature for 10 DFT appeared 9.1, 10.5, 11.6, 13.8, 13.9, 16.2, 16.4, 16.7, 17.1, 17.8℃ depending on the transplanting date. Mean temperature of 9.1℃ and 10.5℃ for 10 DFT appeared in the April 5 and April 15 transplants in 2020 showed negative or no effect on the increase of rice growth and acceleration of heading date when compared to those of right after transplanting treatments in the same year. Mean temperature of 11.6℃ for 10 DFT appeared in the April 5 transplant in 2021 demonstrated greater biomass from early to heading stage but the same heading date compared to April 15 transplant, indicating that 11.6℃ for 10 DFT had a positive effect on rice growth but no effect on advanced heading. Both more biomass and advanced heading stage were observed when the mean temperature for 10 DFT was 13.8℃ or higher, compared to those of right after transplanting treatments. These findings indicate that effective 10-DFT mean temperature for rice growth exists between 10.5 and 11.6℃, and that for rice development in terms of heading stage lies between 11.6 and 13.8℃ in natural condition. Further field and indoor studies are suggested to narrow down the critical temperature for early transplanting of temperate rice, which will enable to maximize the crop period in high altitude regions with low temperature.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1597-1606
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• 2020

Transcription factor engineering to regulate multiple genes has shown promise in the field of microalgae genetic engineering. Here, we report the first use of transcription factor engineering in Chlorella sp. HS2, thought to have potential for producing biofuels and bioproducts. We identified seven endogenous bZIP transcription factors in Chlorella sp. HS2 and named them HSbZIP1 through HSbZIP7. We overexpressed HSbZIP1, a C-type bZIP transcription factor, in Chlorella sp. HS2 with the goal of enhancing lipid production. Phenotype screening under heterotrophic conditions showed that all transformants exhibited increased fatty acid production. In particular, HSbZIP1 37 and 58 showed fatty acid methyl ester (FAME) yields of 859 and 1,052 mg/l, respectively, at day 10 of growth under heterotrophic conditions, and these yields were 74% and 113% higher, respectively, than that of WT. To elucidate the mechanism underlying the improved phenotypes, we identified candidate HSbZIP1-regulated genes via transcription factor binding site analysis. We then selected three genes involved in fatty acid synthesis and investigated mRNA expression levels of the genes by qRT-PCR. The result revealed that the possible HSbZIP1-regulated genes involved in fatty acid synthesis were upregulated in the HSbZIP1 transformants. Taken together, our results demonstrate that HSbZIP1 can be utilized to improve lipid production in Chlorella sp. HS2 under heterotrophic conditions.