• Toxicological Research
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• 제35권3호
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• pp.241-248
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• 2019

Pesticide exposure may induce biochemical alterations including oxidative stress and lipid peroxidation. However, in the context of developmental origin of health and disease, putative trans-generational effect of exposure to pesticides are insufficiently studied. We therefore aimed to evaluate the biochemical effect of gestational exposure to four pesticides on female Wistar rats and their offspring at adult age. We studied 30 female nulliparous Wistar rats divided into 5 equal groups. Group 1 served as the control group and received distilled water while group 2, 3, 4 and 5 received orally pesticide 1 (imidacloprid), pesticide 2 (chlorpyrifos), pesticide 3 (imidacloprid + lambda cyhalothrin) and pesticide 4 (oxamyl) respectively once daily throughout gestation at a dose equivalent to 1/10 lethal dose 50. The mothers were followed up until one month post gestation. The offspring were followed up from birth until adult age (12 weeks). In all animals at each time point we evaluated malondialdehyde (MDA), oxidative stress and liver function enzymes. There was similar variation of total body weight in all the groups during and after gestation. However, Female Wistar rats of the exposed groups had significant alterations in liver SOD (-30.8% to +64.1%), catalase (-38.8% to -85.7%) and GSH (-29.2% to -86.5%) and; kidney catalase (> 100%), GSH (> 100%). Moreover, MDA, alanine transaminase (ALT) and aspartate transaminase (AST) levels were significantly higher in pesticide exposed rats compared to the control group. Similar alterations in antioxidant enzymes, MDA and liver function enzymes were observed in offspring of treated rats evidenced at weaning and persisting until adult age. Exposure to pesticides causes oxidative stress and lipid peroxidation in exposed female Wistar rats and their offspring. The persistence in offspring at adult age suggests transgenerational adverse effects.

• Journal of Oral Medicine and Pain
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• 제41권2호
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• pp.48-53
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• 2016

Purpose: The purpose of this study was to compare the differences in clinical signs and symptoms, and psychological profiles of temporomandibular joint osteoarthritis (TMJ OA) between juvenile and adult patients. Methods: Two-hundred eighty-three TMJ OA patients who visited the Orofacial Pain Clinic of Seoul National University Dental Hospital were classified by juvenile (153 patients; mean age $14.2{\pm}1.7$ years, range 9-16 years) and adult (130 patients; mean age $34.0{\pm}2.8$ years, range 30-40 years) groups, and compared the clinical symptoms based on the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) axis I guidelines including Graded Chronic Pain (GCP) scale, mandibular range of motion, and the associated symptoms. Psychological profiles were also evaluated using the Symptom Checklist 90-Revision (SCL-90-R). Results: Juvenile patients reported lower pain intensity and a lower prevalence of headache and clenching than adult patients. Their mandibular range of motion was also higher than adult patients. Juvenile patients showed a lower percentage of patients with T-score above 50 in somatization (SOM), obsessive-compulsive (O-C), interpersonal sensitivity (I-S), and paranoid ideation (PAR) than adults. Based on the GCP scale, the percentage of the high disability group was lower in juveniles. Conclusions: Juvenile TMJ OA patients generally showed milder clinical symptoms than adults. Adult patients showed higher prevalence of psychological problems and higher disability than juvenile patients. Age should be considered in evaluation and treatment of TMJ OA patients to achieve better treatment results and understanding its pathophysiology.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.247-253
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• 2013

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.217-226
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• 2015

A proper development of the epididymis during the early postnatal development is required for successful fertility in the adult male. Direct cell-cell communication via connexin (Cx) molecules is a common way of cellular interactions to achieve normal development of a given tissue consisting of different cell types. The present research was attempted to determine the effect of exogenous exposure to estrogenic agonist or antiandrogen at the weaning age on expression of Cx isoforms in the adult corpus epididymis. Male rats were subcutaneously administrated with estradiol benzoate (EB) or flutamide (Flu) at the weaning age. The tissue was collected at 4 months of age. Expressional levels of Cx isoforms were determined by a quantitative real-time PCR. Statistical comparison showed significant increases of Cxs31, 32, 37, 40, and 43 transcript amounts by a treatment of $0.015{\mu}g$ of EB /kg body weight (BW). A treatment of $1.5{\mu}g$ of EB /kg BW caused a significant decrease of Cx43 gene expression but increases of Cxs26, 31, 32, 37, and 40 transcript levels. Exposure to $500{\mu}g$ of Flu/kg BW induced an increase of Cx37 expression but significant decreases of Cxs43 and 45 mRNA levels. Expression of Cx37 was increased by a treatment of 5 mg of Flu/kg BW, while transcript levels of Cxs26, 30.3, 31, 31.1, 32, and 43 were significantly decreased by same treatment. These results demonstrate that exposure to steroidal compounds at the early developmental age alters expression of Cx isoforms in the adult corpus epididymis.

• 복식문화연구
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• 제8권5호
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• pp.754-770
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• 2000

The definite objects of this study are as follows ; 1. The study presents concrete objects of the shape of adult women's neck and shoulder after comparing and analyzing the features of five age groups ; the former young age, the latter young age, the former middle age, the latter middle age, the latter middle age and the old age. 2. The study presents concrete objects of adult women's neck and shoulder by the body types ; bend-forward type, straight type and lean-back type in order to be examined the body types. The results of this study are as follows ; 1. The shape of neck and shoulder needs the several concrete objects of each types because of the variable factors in size and body types. But the concrete objects of the types referred in five age groups contain all the important factors and enable to design the body suitable clothes. 2. This study shows that generally bend-forward type contains rising shoulder and lean-back type contains drooping shoulder, and straight type contains average value of neck and shoulder. The results of this study developed the body-suitable clothes of bend-forward type, straight type and lean-back type.

• Applied Microscopy
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• 제16권2호
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• pp.75-91
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• 1986

Differentiation of the rat testis was studied by light and electron microscope from the fetal stage up to the newborn or adult stage. The purpose of the present study is to investigate the ultrastructural changes of seminiferous tubules and interstitial tissue during the developmental process. The results were as follows: the seminiferous tubule diameter began to increase from birth and was fully developed at 30 to 40 days of age through intratubular cell proliferations. Basement membrane and myoid cells lining the seminiferous tubules were differentiated at 17 days gestation. At the fetal stage, seminiferous tubules were primarily composed of Sertoli cells and the differentiation of Sertoli and germ cells progressed from the newborn stage. Spermatids and immature spermatozoa are appeared at 40 days of age, so from this time, spermatogenesis occurred actively until the adult stage. Sertoli cells aided germ cell differentiation and phagocytosed the parts of the spermatid cytoplasm. Leydig ce]] development follows a biphasic pattern: a fetal phase and then an adult phase from 20 days of age. In conclusion, the rat testis is already developed to some extent by the fetal stage and is functional after 50 days of age. Therefore, these findings indicate that differentiation of Sertoli and Leydig cells precedes the onset of spermatogenesis.

• 한국임상수의학회지
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• 제14권1호
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• pp.30-36
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• 1997

It is well estabilished that IL-4, IL-6, $IFN{\gamma}$ and immunoglobulin production are increased as a consequence of advancing age, and endothelial cells increase their expression of pro-inflammatory surface protein, such as ICAM-1, as a consequence of contact with inflammatory cytokines, including IL-1, $TNF{\alpha} or INF{\gamma}.$ This study was designed to define the relationship between age-associated changes of cytokines and immunoglobulin production and ICAM-1 expression in aged mice. Serum from aged mice had elevated IL-6 and immunoglobulin levels compared to mature adult controls and activation-induced production of $IFN{\gamma}$ of splenocytes from aged mice were increased above normal adult level. By immunoperoxidase staining, ICAM-1 in hearts of normal adult mice was expressed occasionally at low levels, but in aged mice, the number of positive blood vessels for ICAM-1 were increased and also the immunoreactivity were stronger. Therefore, these finding indicate that the altered expression of ICAM-1 eith normal aging may actually be correlative to these age-related changes in cytokiness and immunoglobulin production.

• 대한가정학회지
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• 제43권6호
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• pp.13-25
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• 2005

The purpose of this study was to analyse craniofacial shape by 3D scanner for Korean adult head shape by age group. The heads of 836 adults were measured by 3D scanner, and the 25 obtained measurements were analyzed by statifical methods. The results can be summarized as follows. 1. From the basic statistical data analysis, the vertex-tagion and the length between the pupils were the longest in the subjects in their twenties, and decreased in elderly groups. 2. According to the analysis of the craniofacial proportion, the typical adult Korea head type was short-headed. 3. Statistically noticeable differences were found in the ectocanthion- opistcranion by the measurement of the left and the right sides of face in the age groups of the 20s, 30s, 40s, and 50s. 4. The order of factor analysis was as follows: the horizontal length, the perpendicular length and the width from the highest in the age group of the 20s, 30s, 40s, and 50s.

• 대한가정학회지
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• 제40권10호
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• pp.231-241
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• 2002

This study is for research of relationship between shoes wearing by adult woman and foot type. The research was made through questionnaire sheets of general matters (age, body weight, height & occupation) and shoes wearing status (hill height, toe shape, wearing time) of 216 adult women reside in the metropolitan zone and the foot type was classified from foot measurement and the relationship between shoes wearing and foot type was analyzed. According to the result of analysis it is as follows : 1) In the shoes type prefer for they prefer for lower hill as more ages and were preferring shoes with higher hill as the taller women. 2) The instep height is lower and medial foot angle was increased as their ages are more. Majority of foot type was appeared with standard type and the broad type was indicated more by age of 20s. The classification by toe the square type was indicated by majority and in the age of 30-40s the egyptian type was more 3) As the age and weight get increased he height of arch become lower and indicated to transit to flat-foot. That is to say they prefer for round toe in case of fiat-foot, and for square toe in case of normal and, choice of shoe toe shape it indicated by more impact by the height of arch rather than toe length.

• 한국의류산업학회지
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• 제19권1호
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• pp.49-60
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• 2017

In this study, change trends and specific times for change in body size were sought to be identified by using manual measurements for Korean adult women at 20 to 89 of age, along with examinations of change trends and specific times of change in body shape based on body index values. The examinations of means, deviations, and variation coefficients revealed that there was a tendency of most height related measures to decrease. In addition, combined with decrease in height, growth in weight led to increased BMI, thus resulting in increase in measures of circumference, thickness, and breadth in the torso area. The BMI trends showed that with increasing age, BMI increased, reaching the peak between the ages of 55 and 59 and then decreasing from 64 of age, with the steepest increase in BMI at ages from 50 to 54. The examination of differences in the body flatness ratio for different parts of body found that with increasing age the ratio decreased, indicating a tendency for gradual change to round body shapes. The analysis of 3D body shape found that there were more back-bends and lowered shoulder levels with age, accompanied with a tendency for more bended legs and more inclined-forward necks. The key trends in changes in body measurements by age and detailed estimates of 3D standard body shape drawn out from this study are expected to significantly contribute to establishing a basic database for generating patterns reflecting age features of Korean adult females.