• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3507-3511
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• 2012

Objective: The aim of this study was to investigate possible mechanisms of LOX gene effects on invasion and metastasis of breast cancer cells by RNA interference. Methods: LOX-RNAi-LV was designed, synthesized, and then transfected into a breast cancer cell line (MDA-MB-231). Expression of LOX, MMP-2 and MMP-9 was determined by real-time PCR, and protein expression of LOX by Western blotting. Cell migration and invasiveness were assessed with Transwell chambers. A total of 111 cases of breast cancer tissues, cancer-adjacent normal breast tissues, and 20 cases of benign lesion tissues were assessed by immunohistochemistry. Results: Expression of LOX mRNA and protein was suppressed, and the expression of MMP-2 and MMP-9 was significantly lower in the RNAi group than the control group (P<0.05), after LOX-RNAi-LV was transfection into MDA-MB-231 cells. Migration and invasion abilities were obviously inhibited. The expression of LOX protein in breast cancer, cancer-adjacent normal breast tissues and benign breast tumor were 48.6% (54/111), 26.1% (29/111), 20.0% (4/20), respectively, associations being noted with clinical stage, lymph node metastasis, tumor size and ER, PR, HER2, but not age. LOX protein was positively correlated with MMP-2 and MMP-9. Conclusion: LOX displayed an important role in invasion and metastasis of breast cancer by regulating MMP-2 and MMP-9 expression which probably exerted synergistic effects on the extracellular matrix (ECM).

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2016년도 춘계학술대회
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• pp.641-644
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• 2016

유선 네트워크와 달리 무선 네트워크 환경에서의 브로드캐스팅은 단일 전송으로 다수의 노드들에게 데이터를 한 번에 송신할 수 있다. 즉, 무선 네트워크에서 특정 노드의 전방향적 브로드캐스팅은 인접한 모든 노드들에게 동시에 도달한다. 본 논문에서는 무선 네트워크에서 브로드캐스팅 시 전체 송신 전력을 최소화하는 최소 전력 브로드캐스팅 문제를 해결하기 위한 탐욕 알고리즘을 제안한다. 제안된 알고리즘은 각 노드간의 거리로 구성된 거리 행렬과 인접 노드의 수를 갖는 인접 행렬을 이용한다. 데이터를 수신한 노드 중 인접 노드를 가장 많이 가지고 있는 노드가 우선적으로 데이터를 전송한다. 제안된 알고리즘의 성능평가는 컴퓨터 시뮬레이션을 이용하여 네트워크상의 모든 노드로 브로드캐스팅 시 소요되는 송신 전력 관점에서 랜덤 방식과 비교하며, 실험 결과에서 제안된 알고리즘은 랜덤 방식보다 성능이 우수함을 보인다.

• 예술인문사회 융합 멀티미디어 논문지
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• 제8권8호
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• pp.825-834
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• 2018

방송은 상호연결망에서 사용되는 가장 기본적인 정보전달 기법으로 크게 일-대-다 방송과 다-대-다 방송으로 나눌 수 있다. 일-대-다 방송은 메시지를 갖고 있는 한 노드에서 다른 모든 노드로 메시지를 전송하는 것이고, 다-대-다 방송은 메시지를 갖고 있는 각각의 노드들이 다른 모든 노드들로 메시지를 전송하는 것이다. 그리고 단위 시간 당 전송 포트를 사용하는 방법에 따라 단일 포트 통신 방식(SLA)과 멀티 포트 통신 방식(MLA)으로 나눌 수 있다. 단일 포트 통신 방식은 단위 시간에 메시지를 가지고 있는 노드가 이웃한 다른 하나의 노드로만 메시지를 전송하는 것이고, 멀티 포트 통신 방식은 단위 시간에 메시지를 가지고 있는 노드가 이웃한 모든 노드로 메시지를 전송하는 것이다. 매트릭스 하이퍼큐브는 하이퍼큐브와 동일한 노드 개수를 가지면서 하이퍼큐브보다 망비용이 개선된 연결망이다. 본 논문에서는 매트릭스 하이퍼큐브의 방송 기법을 분석한다. 먼저 매트릭스 하이퍼큐브에서의 일-대-다 방송 알고리즘과 다-대-다 방송 알고리즘을 제안한다. 그리고 SLA 기법을 이용한 일-대-다 방송 시간이 2n+1임과 MLA 기법을 이용한 일-대-다 방송 시간이 $2{\lceil}{\frac{n}{2}}{\rceil}+1$임을 보인다. 또한 SLA 기법을 이용한 다-대-다 방송 시간이 $5{\times}2^{\frac{n}{2}}-2$(n=짝수), $5{\times}2^{\frac{n-1}{2}}+2$(n=홀수)임을 증명한다.

• 대한치과보철학회지
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• 제32권4호
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• pp.593-607
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• 1994

The purpose of this study is to evaluate the effect of the bone morphogenetic protein, bone matrix gelatin and collagen matrix on the amount and shape of generating new bone adjacent to the implant. Implants were inserted in the mandible of adult dogs at 2 months after teeth extraction. Artificial bony defects, 3mm in width and 4mm in depth were made at the mesial and distal side of implant. Experimental groups were divided into three groups ; Group 1 : Defects filled with collagen matrix and bone morphogenetic protein, Group 2 : Defects filled with bone matrix gelatin. Control group : Defects filled with only collagen matrix. After implantation, the animals were sacrificed at 1,3,5 and 10 weeks for light microscopic examination. For the fluorescent microscopic examination. each tertracycline Hcl and calcein were injected at 1, 3, 5, 8 and 10 weeks after implantation. The results obtained were as follows : 1. The molecular weight of bovine BMP was about 18,100 by hydroxyapatite chromatography. 2. Osseointegration was observed in experimental groups 1 & 2, and BMG and BMP had an excellent bone forming capability as a filling materials to the repair of the bone defects. 3. The degree of healing of bone defect area, the experimental group 1 showed more prominent bone formation than control group, and the control group showed fibrous connective tissue between the implant and the bone. 4. In the fluorescent microscopic findings, bone remodelling was observed regenerative lamellar bone at defect area in experimental group 1, and partial remodelling in experimental group 2, In the control group, fibrous connective tissue was observed between the implant and bone surface and sign of remodelling was not apperaed. Above results suggest that BMP has rapid osteoinductive property and can be used clinically as a bone substitute on bone defects around implants.

• Journal of Yeungnam Medical Science
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• 제23권1호
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• pp.82-89
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• 2006

최근 여러 종양에서 단백분해효소의 분비와 암의 악성도에 대한 연구가 이루어져 왔으며, 이를 신세포암종 환자의 예후 측정인자로 사용하려는 시도가 진행되고 있다. 이에 저자들은 제 4형 collagenase 중 대표적인 MMP-9의 발현정도를 정상 신조직과 신세포암종 조직에서 비교하였고, 또 암의 침윤 및 전이정도와의 관계와 다른 임상적 인자들과의 상관성을 분석하여 암의 단계적 진행과정에서의 MMP-9의 발현변화에 대하여 조사하였다. 그 결과 정상 신 조직에 비해 신세포암종 조직에서 MMP-9의 발현이 증가되며 암의 크기가 크고 혈관침범이 있으며 병기가 높을수록 MMP-9의 발현이 증가됨을 관찰할 수 있었다. 이는 MMP-9 발현의 증가가 신세포암종의 발생과정 및 암의 후기 진행에 관여함을 시사하므로 향후 신세포암종의 예후척도로 사용되어 치료방침을 결정하는데 도움을 줄 수 있을 것으로 생각한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권5호
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• pp.386-391
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• 2010

Introduction: Bone resorption is a unique function of osteoclasts. Osteoclasts are a specialized macrophage polykaryon whose differentiation is regulated principally by macrophage colony-stimulating factors, receptor activator of nuclear factor ${\kappa}B$ ligand (RANK) ligand, osteoprotegerin (OPG), and interleukins (IL). Reflecting the integrin-mediated signals, osteoclasts develop a specialized cytoskeleton that allows it to establish an isolated micro-environment between itself and the bone, wherein matrix degradation occurs by a process involving proton transport. The levels of IL-1, IL-6, OPG, and prostaglandin $E_2$ ($PGE_2$) expression were evaluated to study the correlations between dental implant teeth and the adjacent teeth. Materials and Methods: The exudate of the gingival crevice acquired from dental implants, adjacent teeth, opposite teeth and contralateral teeth of 24 patients. Results: 1. The levels of IL-1, IL-6, OPG and $PGE_2$ expression in dental implant teeth were higher than those of the contralateral teeth. 2. IL-1 revealed a higher expression level in the adjacent teeth than in dental implant teeth. 3. The dental implant teeth and adjacent teeth did not show a remarkable difference in the level of IL-1 expression. 4. All the other cytokines were strongly expressed in the dental implant compared to the adjacent teeth. Conclusion: These results suggest that there might be close correlation between dental implant teeth and adjacent teeth in terms of the expressions of cytokines that affect the development and regulation of osteoclasts.

• Journal of Microbiology
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• 제43권5호
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• pp.411-416
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• 2005

The high-throughput sequencing of microbial genomes has resulted in the relatively rapid accumulation of an enormous amount of genomic sequence data. In this context, the problem posed by the detection of promoters in genomic DNA sequences via computational methods has attracted considerable research attention in recent years. This paper addresses the development of a predictive model, known as the dependence decomposition weight matrix model (DDWMM), which was designed to detect the core promoter region, including the -10 region and the transcription start sites (TSSs), in prokaryotic genomic DNA sequences. This is an issue of some importance with regard to genome annotation efforts. Our predictive model captures the most significant dependencies between positions (allowing for non­adjacent as well as adjacent dependencies) via the maximal dependence decomposition (MDD) procedure, which iteratively decomposes data sets into subsets, based on the significant dependence between positions in the promoter region to be modeled. Such dependencies may be intimately related to biological and structural concerns, since promoter elements are present in a variety of combinations, which are separated by various distances. In this respect, the DDWMM may prove to be appropriate with regard to the detection of core promoter regions and TSSs in long microbial genomic contigs. In order to demonstrate the effectiveness of our predictive model, we applied 10-fold cross-validation experiments on the 607 experimentally-verified promoter sequences, which evidenced good performance in terms of sensitivity.

• Steel and Composite Structures
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• 제24권6호
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• pp.727-740
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• 2017

The present paper studies the stability analysis of the continuously graded CNT-Reinforced Composite (CNTRC) panel stiffened by rings and stringers. The Stiffened Panel (SP) subjected to axial and lateral loads is reinforced by agglomerated CNTs smoothly graded through the thickness. A two-parameter Eshelby-Mori-Tanaka (EMT) model is adopted to derive the effective material moduli of the CNTRC. The stability equations of the CNRTC SP are obtained by means of the adjacent equilibrium criterion. Notwithstanding most available literature in which the stiffener effects were smeared out over the respective stiffener spacing, in the present work, the stiffeners are modeled as Euler-Bernoulli beams. The Generalized Differential Quadrature Method (GDQM) is employed to discretize the stability equations. A numerical study is performed to investigate the influences of different types of parameters involved on the critical buckling of the SP reinforced by agglomerated CNTs. The results achieved reveal that continuously distributing of CNTs adjacent to the inner and outer panel's surface results in improving the stiffness of the SP and, as a consequence, inclining the critical buckling load. Furthermore, it has been concluded that the decline rate of buckling load intensity factor owing to the increase of the panel angle is significantly more sensible for the smaller values of panel angle.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권1호
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• pp.24-39
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• 2000

Various methods and graft materials have been used to fill in the defect adjacent to the implants and considered as clinically acceptable. But it is not clear whether the regenerated bone increases the implant-bone contact and supports the implant. The purpose of this study is to evaluate regenerated bone surrounding implants using bone morphogenetic protein(BMP) and demineralized freeze-dried bone(DFDB), and the interfaces between implants and regenerated bone. bBMP was extracted and partially purified from the bovine bone matrix using heparine chromatography. Demineralized freeze-dried bone was made from the dog. Inactive insoluble collagenous bone matrix(IBM) of dog was used as carrier of bBMP. Interfaces of titanium coated epoxy resin implants were processed for demineralized section for transmission electron microscopy(TEM) and those of screw type implants were for nondemineralized section for light and fluoromicroscopic examination. Implants were inserted in the inferior border of mandible of adult dogs and artificial bony defects($3{\times}3{\times}4mm$) were made at the mesial and distal side of implants. Defects were filled with BMP(BMP group) and DFDB(DFDB group). For the fluoromicroscopic examination, the fluorescent dyes(oxytetracycline, calcein green, alizarin red) were injected 2, 4, 6, 8, 12 weeks after implantation. The experimental animals were sacrificed at the 6th and the 12th week and their mandible were extirpated and processed for examination with light microscopy, fluoromicroscopy and TEM. The obtained results were as follows : 1. By the light microscopic findings, the defects were filled with woven bone at the 6th week and compact bone at the 12th week, and the osseointegrations were seen in both groups. There was no histological difference between them. 2. On the basis of the histomorphometric analysis, BMP group(6th week: 40.25%, 12th week: 56.04%) had higher bony contact ratio than DFDB group(38.37%, 42.63%). There was significant difference between two groups at the 12th week(p<0.05). 3. The amount of bone formation in BMP group was more prominent than in DFDB group. Significant difference was noted among two groups at the 6th and the 8th week(p<0.05). 4. By the transmission electron microscopic findings, $0.4-2{\mu}m$ soft tissue layer was found in adjacent to the interfaces and over the collagen fibrils of bone at the 6th week. However, about 100nm amorphous layer was noted at the interface or collagen fibrils directly extended to the titanium surface at the 12th week. There was no significant difference between two groups. 5. These results suggest that BMP and DFDB can be used as good graft materials in the regeneration of bone adjacent to implant, and BMP is more valuable as a bone inducer than DFDB.

• 대한기계학회논문집A
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• 제28권9호
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• pp.1284-1296
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• 2004

In order to investigate effects of anisotropic fiber packing on stresses in composites, a Volume Integral Equation Method is applied to calculate the elastostatic field in an unbounded isotropic elastic medium containing multiple orthotropic inclusions subject to remote loading, and a Mixed Volume and Boundary Integral Equation Method is introduced for the solution of elastostatic problems in unbounded isotropic materials containing multiple anisotropic inclusions as well as one void under uniform remote loading. A detailed analysis of stress fields at the interface between the isotropic matrix and the central orthotropic inclusion is carried out for square, hexagonal and random packing of orthotropic cylindrical inclusions, respectively. Also, an analysis of stress fields at the interface between the isotropic matrix and the central orthotropic inclusion is carried out, when it is assumed that a void is replaced with one inclusion adjacent to the central inclusion of square, hexagonal and random packing of orthotropic cylindrical inclusions, respectively, due to manufacturing and/or service induced defects. The effects of random orthotropic fiber packing on stresses at the interface between the isotropic matrix and the central orthotropic inclusion are compared with the influences of square and hexagonal orthotropic fiber packing on stresses. Through the analysis of plane elastostatic problems in unbounded isotropic matrix with multiple orthotropic inclusions and one void, it will be established that these new methods are very accurate and effective for investigating effects of general anisotropic fiber packing on stresses in composites.