• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.273-276
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• 2004

The adhesion of hydrocarbon degrading bacteria(HDB) differing in surface hydrophobicity was investigated. Cell wall hydrophobicity was modified chemically and physiologically. Modified adhesion deficient mutant of HDB was selected in a soil column assay. Physiologically and chemical modification increased cell surface hydrophobicity. Cell surface characteristics including BATH and FTIR were measured. Physiological modification using ampicillin was not stable, but chemical modification was stable. Hydrocarbon degrading efficiency was measured of TPH modified and unmodifed HDB.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 추계학술발표회
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• pp.360-362
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• 2003

The adhesion of hydrocarbon degrading bacteria(HDB) differing in surface hydrophobicity was investigated. Cell wall hydrophobicity was modified chemically and physiologically. Modified adhesion deficient mutant of HDB was selected in a soil column assay Physiologically and chemical modification increased cell surface hydrophobicity. Cell surface charcteristis including BATH and zeta potential were measured. Physiological modification using ampicillin was not stable, but chemical modification was stabel. Hydrocarbon degrading potential was measured for modified and unmodifed HDB.

• 한국초지조사료학회지
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• 제34권1호
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• pp.60-67
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• 2014

본 연구는 조사료의 반추위 발효가 진행됨에 따른 볏짚표면에 부착된 섬유소 분해 박테리아의 군집변화와 섬유소 소화율을 비교 관측하기 위하여 볏짚의 in situ 반추 발효를 실시하였다. 그리고 부착 박테리아의 군집 변화를 측정하기 위하여 RT-PCR 기법을 이용하여 F. succinogenes. R. albus와 R. flavefaciens의 군집을 모니터링 하였다. 본 연구를 수행하기 위하여 in situ 볏짚 발효를 0. 2, 4, 8, 12, 24시간 실시하였을 때 반추위내 볏짚의 in situ 분해는 발효 시간이 진행됨에 따라 가속화되어 발효 8~12시간 사이에 최고 분해 속도를 나타내었으나, F. succinogenes, R. flavefaciens과 R. albus는 모두 발효 0~1시간 사이에 볏짚 표면에 부착이 80% 이상 완료되어 이후 발효가 계속 진행되는 동안 일정 수준의 군락을 유지하는 것이 발견되었다. 그리고 반추위내 유입된 조사료의 표면에 초기 부착과정을 관찰하기 위하여 0, 5, 10, 30 및 60분 간격으로 볏짚의 in situ 샘플을 채취하여 조사하였을 때 F. succinogenes, R. flavefaciens 및 R. albus의 군락 모두 볏짚이 반추위 유입 후 5분 내에 상당량의 수가 부착함을 발견하였다. 또한 조사료의 반추위 발효 용이성에 따른 섬유소 분해 박테리아의 부착 정도를 관찰하기 위하여 0, 2, 4 및 8% NaOH를 처리한 볏짚을 12 및 24시간 in situ 배양 볏짚의 소화율과 부착 박테리아의 군집 변화를 관측하였을 때, 볏짚의 NaOH 처리 농도가 높아짐에 따라 in situ 소화율이 증가하였으며, 동시에 부착된 박테리아 군집의 증가 경향이 F. succinogenes, R. flavefaciens 및 R. albus의 3균주 모두 배양 12시간에 나타났으나 배양 24시간에서는 각기 다른 양상을 나타냈다. 따라서 본 연구결과는 반추위내 섬유소 발효과정에서 섬유소 분해 박테리아의 부착은 조사료의 반추위 유입 초기에 반드시 이루어지고, 발효 시간이 진행됨에 따라 조사료 표면에 안정된 군락을 형성하며, 섬유소 분해가 가속화된다는 사실을 보여 주었다.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.178-186
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• 2009

To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

• 대한치과보철학회지
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• 제42권4호
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• pp.373-385
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• 2004

Statement of problem: The microbial adhesion on the surface of materials used in prosthodontics and restorative dentistry significantly influences microbial infection. Purpose: The purpose of this study was to evaluate the effect of how the degree of surface roughness of acrlyic resin affect the adhesion of bacteria. Material and methods: Resins were finished with $50{\mu}m$ and $250{\mu}m$ aluminium oxide particles by using sandblaster, by using stone point, and high polished with $Opa^{(R)}$ and Lace $motor^{(R)}$. The surface of acrylic resin attached by bacteria was directly touched on the surface of BHI agar, which was incubated. Bacteria colonies formed on BHI agar were counted in accordance with the degree of the surface roughness. Results: 1. The viable cell number of Streptococcus mutans increased on the acrylic resins incubated in BHI broth than in PBS. 2. The viable cell number of Streptococcus mutans increased on the acrylic resins incubated without agitation than with agitation, washed three times than six times, and incubated in broth added with 5% sucrose than without sucrose. 3. When Streptococcus mutans incubated in BHI broth, the number of Streptococcus mutans colonies formed on BHI agar was the largest on the acrylic resins finished with $250{\mu}m$ aluminium oxide particle using sandblaster. But when incubated in BHI broth containing sucrose, the number of colonies formed on that was the largest on the acrylic resins high polished using $Opal^{(R)}$ and Lace $motor^{(R)}$. 4. When Streptococcus sanguis was incubated in BHI broth with or without sucrose, the number of Streptococcus mutans colonies formed on BHI agar was the largest on the acrylic resins finished with $250{\mu}m$ aluminium oxide particle using sandblaster. 5. When Actinomyces viscosus was incubated in BHI broth with or without sucrose, the number of Streptococcus mutans colonies formed on BHI agar was the largest on the acrylic resins high polished using $Opal^{(R)}$ and Lace $motor^{(R)}$. Conclusion: These results indicated that when acrylic resins attached by bacteria were touched on the surface of BHI agar, the number of bacterial colonies formed on the agar was dependent on the bacterial species. Also, the result of this study was showed that increase in the surface roughness and the addition of sucrose increased retention of microbial cells.

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1731-1739
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• 2012

In this study, seven strains isolated from mustard leaf kimchi were screened for their tolerance to simulated gastric and bile juices, the adhesive properties to Caco-2 cells, and the inhibition ability of Salmonella Typhimurium ATCC 29631 adhesion. Lactobacillus acidophilus GK20, Lactobacillus paracasei GK74, and Lactobacillus plantarum GK81, which were resistant to bile as well as gastric juices, possessed high bile-salt hydrolase (BSH) activity towards both sodium glycocholate and sodium taurocholate. The strongest in vitro adherence of $53.96{\pm}4.49%$ was exhibited by L. plantarum GK81 followed by L. acidophilus GK20 with adhesion levels of $40.72{\pm}9.46%$. The adhesion of these strains was significantly (p < 0.05) reduced after exposure to pepsin and heating for 30 min at $80^{\circ}C$. Addition of $Ca^{2+}$ led to a significant (p < 0.05) increase of the adhesion of L. acidophilus GK20, but the adhesion ability of L. plantarum GK81 was not different from the control by the addition of calcium. In the competition and exclusion experiment, the adhesion inhibition of S. Typhimurium by L. plantarum GK81 strain was much higher than the other strains. Moreover, the exclusion inhibition of S. Typhimurium by L. acidophilus GK20 was considerably high, although the inhibition activity of this strain was lower than L. plantarum GK81.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.592-600
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• 2021

Probiotics can be processed into a powder, tablet, or capsule form for easy intake. They are exposed to frequent stresses not only during complex processing steps, but also in the human body after intake. For this reason, various coating agents that promote probiotic bacterial stability in the intestinal environment have been developed. Silk fibroin (SF) is a material used in a variety of fields from drug delivery systems to enzyme immobilization and has potential as a coating agent for probiotics. In this study, we investigated this potential by coating probiotic strains with 0.1% or 1% water-soluble calcium (WSC), 1% SF, and 10% trehalose. Under simulated gastrointestinal conditions, cell viability, cell surface hydrophobicity, and cell adhesion to intestinal epithelial cells were then measured. The survival ratio after freeze-drying was highest upon addition of 0.1% WSC. The probiotic bacteria coated with SF showed improved survival by more than 10.0% under simulated gastric conditions and 4.8% under simulated intestinal conditions. Moreover, the cell adhesion to intestinal epithelial cells was elevated by 1.0-36.0%. Our results indicate that SF has positive effects on enhancing the survival and adhesion capacity of bacterial strains under environmental stresses, thus demonstrating its potential as a suitable coating agent to stabilize probiotics throughout processing, packaging, storage and consumption.

• 생명과학회지
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• 제33권4호
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• pp.334-342
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• 2023

가자미식해에서 분리된 유산균 40종은 Lactobacillus plantarum spp., Leuconostoc mesenteroides spp., Lactobacillus brevis spp., and Weisella paramenteroides spp.로 확인됐으며, 40종 중 10종을 선별해 검사에 사용했다. 본 연구에서는 가자미식해에서 분리된 유산균을 프로바이오틱스로 활용하기 위해 산 및 인공위액 저항성, 담즙산 저항성, 자가 응집성, 공동 응집성, 세포 표면 소수성 등의 프로바이오틱 연구를 수행하였다. 분리한 유산균주는 산성 및 인공 위액에 대해 저항성을 보여 높은 생존율을 나타내었으며, L. plantarum GS11이 가장 뛰어난 저항성을 보였다. 또한 담즙산 저항성 측정 결과 모든 유산균주가 10⁸~10⁹ log CFU/ml의 생균수로, 100% 이상의 생존력을 보여주었다. 그리고 세포 표면 부착능을 간접적으로 측정하기 위해 응집력 평가를 한 결과, autoaggregation 능력을 46% 이상 나타내었다. 세포 표면 소수성 평가를 위해 Xylene 부착능을 측정한 결과 분리된 유산균주에서 32.2%의 소수성을 가지는 B. subtilis 보다 더 뛰어난 세포 부착률을 보여주었다. 이와같이 유산균은 프로바이오틱스로 활용될 만한 유의미한 결과를 보였으며, L. plantarum GS12와 L. plantarum GS13을 제외한 유산균에서 항균 활성이 나타났다. 따라서 가자미 식해에서 분리된 유산균은 다양한 프로바이오틱스 특성을 가진 프로바이오틱스로 활용할 수 있을 것으로 판단되어진다.

• 치위생과학회지
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• 제18권2호
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• pp.69-75
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• 2018

우리 연구의 목적은 DUWL에서 배출되는 물에서 분리한 균주의 부착 능력과 바이오필름 형성 능력을 확인하고 두 능력 사이 관계를 확인하는 것이다. DUWL로부터 분리한 12균주를 실험에 사용하였다. 각 균주의 부착 능력을 확인하기 위해, 12-well plates의 각 well에 멸균된 glass coverslip, R2A 액체 배지, 그리고 $1{\times}10^8CFU/ml$의 농도로 조정된 세균 현탁액을 넣고 $26^{\circ}C$ 배양기에서 7일 동안 배양하였다. 배양 후, glass coverslip에 부착한 정도에 따라 1~3점으로 점수를 부여하였다. 분리 균주의 바이오필름 형성 능력을 확인하기 위해, 96-well polystyrene flat-bottom microtiter plate에 R2A 액체 배지와 세균 현탁액을 넣고 $26^{\circ}C$에서 7일 동안 배양하였다. 배양 후, plate에 형성된 바이오필름은 R2A 액체 배지에 현탁했고, 현탁액을 R2A 고체 배지에 도말하였다. $26^{\circ}C$에서 7일 배양한 후 세균의 집락을 계수하고 CFU/ml를 계산하였다. DUWL로부터 총 56균주가 분리되었으며, 12속과 31종을 포함하였다. 실험에는 속당 1균주씩 선택하여 총 12균주가 사용되었다. 12균주 중에 S. echinoides, M. aquaticum, C. pauculus의 부착 능력 점수는 +3으로 가장 높았다. 바이오필름 축적량은 C. pauculus가 가장 많았고, M. testaceum이 가장 적었다. 대부분의 부착 능력이 높은 균주는 바이오필름 형성 능력 또한 높았다. 본 연구의 결과는 DUWL 바이오필름의 형성 기전을 파악하는데 도움을 주며 나아가 바이오필름 형성을 억제하는 방법의 개발에 기본적인 정보를 제공할 수 있을 것이다.

• The Journal of Advanced Prosthodontics
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• 제3권2호
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• pp.81-84
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• 2011

PURPOSE. The aim of this in vitro study was to investigate the adhesion of initial colonizer, Streptococcus sanguis, on resin, titanium and zirconia under the same surface polishing condition. MATERIALS AND METHODS. Specimens were prepared from Z-250, cp-Ti and 3Y-TZP and polished with $1 {\mu}m$ diamond paste. After coating with saliva, each specimen was incubated with Streptococcus sanguis. Scanning electron microscope, crystal violet staining and measurement of fluorescence intensity resulting from resazurin reduction were performed for quantifying the bacterial adhesion. RESULTS. Surface of resin composite was significantly rougher than that of titanium and zirconia, although all tested specimens are classified as smooth. The resin specimens showed lower value of contact angle compared with titanium and zirconia specimens, and had hydrophilic surfaces. The result of scanning electron microscopy demonstrated that bound bacteria were more abundant on resin in comparison with titanium and zirconia. When total biofilm mass determined by crystal violet, absorbance value of resin was significantly higher than that of titanium or zirconia. The result of relative fluorescence intensities also demonstrated that the highest fluorescence intensity was found on the surface of resin. Absorbance value and fluorescence intensity on titanium was not significantly different from those on zirconia. CONCLUSION. Resin specimens showed the roughest surface and have a significantly higher susceptibility to adhere Streptococcus sanguis than titanium and zirconia when surfaces of each specimen were polished under same condition. There was no significant difference in bacteria adhesion between titanium and zirconia in vitro.