• International Journal of Oral Biology
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• 제33권2호
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• pp.71-76
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• 2008

The neurotrophin plays an important role in the development, differentiation and survival of the nervous system in vertebrates. It exerts its cellular effects through two different receptors, the Trk receptor tyrosine kinase neurotrophin receptor and the p75 neurotrophin receptor, a member of the tumor necrosis factor receptor superfamily. Trk and p75 neurotrophin receptors utilize specific target proteins to transmit signals into the cell. An ankyrin-rich membrane spanning protein (ARMS) was identified as a new p75 interacting protein and serves as a novel downstream target of p75 neurotrophin receptor. We sought to delineate the interaction between p75 and ARMS by deletion constructs of p75 and green fluorescent protein (GFP)-tagged ARMS. We examined the interaction between these two proteins after overexpressing them in HEK-293 cells. Using both Western blot analysis and immunocytochemistry followed by confocal laser scanning microscopy, we found out that the intracellular domain of the p75 neurotrophin receptor was important for the interaction with ARMS. The results from this study suggest that ARMS may play an important role for mediating the signals from p75 neurotrophin receptor into the cell.

• 항공우주기술
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• 제6권2호
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• pp.111-118
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• 2007

현재 개발 중인 KSLV-I은 2단으로 구성된 발사체이며, 1/2단은 어댑터 링을 사용하여 연결한다. 단 조립 후에 1/2단 통신을 위해 사용되는 케이블은 외부로 노출된다. 또한 1/2단 분리를 위하여 어댑터 링에 장착되는 8개의 파이로 볼트도 외부로 노출된다. 이처럼 외부로 노출되는 케이블이나 작동 시 파편 및 충격으로 인한 피해가 우려되는 부분에는 부품과 구조물을 보호하기 위해 일반적으로 카울을 사용하고 있다. KSLV-에서는 용도에 따라 2종의 카울(엄비리컬 카울, 파이로 볼트 카울)을 설계하였다. 카울은 외부로 노출되었기 때문에 공기 유동으로 발생되는 열과 압력 하중을 받게 된다. 따라서 카울은 열과 압력 하중에 대한 구조 강도를 해석을 통하여 검증하여야 한다. 본 연구에서는 열 하중을 제외한 압력 하중에 대한 구조 해석을 수행하였다. 구조 해석 결과 파이로 볼트 카울은 구조해석 결과로부터 구조 강도면에서 안전함을 확인하였지만, 엄비리컬 카울의 경우 주어진 압력하중으로 인해 파손됨을 확인하였다. 하중 조건을 만족시킬 수 있도록 엄비리컬의 카울의 형상을 변경하였으며 추가적으로 구조 해석을 수행하여 하중 조건을 만족함을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2355-2362
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• 2012

The SH2B1 adaptor protein is recruited to multiple ligand-activated receptor tyrosine kinases that play important role in the physiologic and pathologic features of many cancers. The purpose of this study was to assess SH2B1 expression and to explore its contribution to the non-small cell lung cancer (NSCLC). Methods: SH2B1 expression in 114 primary NSCLC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. Additionally, 15 paired NSCLC background tissues, 5 NSCLC cell lines and a normal HBE cell line were evaluated for SH2B1 expression by RT-PCR and immunoblotting, immunofluorescence being applied for the cell lines. Results: SH2B1 was found to be overexpressed in NSCLC tissues and NSCLC cell lines. More importantly, high SH2B1 expression was significantly associated with tumor grade, tumor size, clinical stage, lymph node metastasis, and recurrence respectively. Survival analysis demonstrated that patients with high SH2B1 expression had both poorer disease-free survival and overall survival than other patients. Multivariate Cox regression analysis revealed that SH2B1 overexpression was an independent prognostic factor for patients with NSCLC. Conclusions: Our findings suggest that the SH2B1 protein may contribute to the malignant progression of NSCLC and could offer a novel prognostic indicator for patients with NSCLC.

• Molecules and Cells
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• 제40권10호
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• pp.706-713
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• 2017

Osteoclasts are bone-resorbing cells that are derived from hematopoietic precursor cells and require macrophage-colony stimulating factor and receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) for their survival, proliferation, differentiation, and activation. The binding of RANKL to its receptor RANK triggers osteoclast precursors to differentiate into osteoclasts. This process depends on RANKL-RANK signaling, which is temporally regulated by various adaptor proteins and kinases. Here we summarize the current understanding of the mechanisms that regulate RANK signaling during osteoclastogenesis. In the early stage, RANK signaling is mediated by recruiting adaptor molecules such as tumor necrosis factor receptorassociated factor 6 (TRAF6), which leads to the activation of mitogen-activated protein kinases (MAPKs), and the transcription factors nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and activator protein-1 (AP-1). Activated NF-${\kappa}B$ induces the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), which is the key osteoclastogenesis regulator. In the intermediate stage of signaling, the co-stimulatory signal induces $Ca^{2+}$ oscillation via activated phospholipase $C{\gamma}2$ ($PLC{\gamma}2$) together with c-Fos/AP-1, wherein $Ca^{2+}$ signaling facilitates the robust production of NFATc1. In the late stage of osteoclastogenesis, NFATc1 translocates into the nucleus where it induces numerous osteoclast-specific target genes that are responsible for cell fusion and function.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.457-468
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• 2022

It has been demonstrated that APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is involved in the regulation of several growth-related signaling pathways and thus closely associated with the development and progression of some cancers. Diallyl trisulfide (DAT), a garlic-derived bioactive compound, exerts selective cytotoxicity to various human cancer cells through interfering with pro-survival signaling pathways. However, whether and how DAT affects survival of human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we tested the hypothesis of the involvement of APPL1 in DAT-induced cytotoxicity in HCC HepG2 cells. We found that Lys 63 (K63)-linked polyubiquitination of APPL1 was significantly decreased whereas phosphorylation of APPL1 at serine residues remained unchanged in DAT-treated HepG2 cells. Compared with wild-type APPL1, overexpression of APPL1 K63R mutant dramatically increased cell apoptosis and mitigated cell survival, along with a reduction of phosphorylation of STAT3, Akt, and Erk1/2. In addition, DAT administration markedly reduced protein levels of intracellular TNF receptor-associated factor 6 (TRAF6). Genetic inhibition of TRAF6 decreased K63-linked polyubiquitination of APPL1. Moreover, the cytotoxicity impacts of DAT on HepG2 cells were greatly attenuated by overexpression of wild-type APPL1. Taken together, these results suggest that APPL1 polyubiquitination probably mediates the inhibitory effects of DAT on survival of HepG2 cells by modulating STAT3, Akt, and Erk1/2 pathways.

• International Journal of Oral Biology
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• 제47권3호
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• pp.33-40
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• 2022

Store-operated Ca²⁺ entry (SOCE) represents one of the major Ca²⁺ entry routes in non-excitable cells. It is involved in a variety of fundamental biological processes and the maintenance of Ca²⁺ homeostasis. The Ca²⁺ release-activated Ca²⁺ (CRAC) channel consists of stromal interaction molecule and Orai; however, the role and action of Homer proteins as an adaptor protein to SOCE-mediated Ca²⁺ signaling through the activation of CRAC channels in non-excitable cells still remain unknown. In the present study, we investigated the role of Homer2 in the process of Ca²⁺ signaling induced by the interaction between CRACs and Homer2 proteins in non-excitable cells. The response to Ca²⁺ entry by thapsigargin-mediated Ca²⁺ store depletion remarkably decreased in pancreatic acinar cells of Homer2^-/- mice, as compared to wild-type cells. It also showed critical differences in regulated patterns by the specific blockers of SOCE in pancreatic acinar cells of Homer2^-/- mice. The response to Ca²⁺ entry by the depletion in Ca²⁺ store markedly increased in the cellular overexpression of Orai1 and STIM1 as compared to the overexpression of Homer2 in cells; however, this response was remarkably inhibited by the overexpression of Orai1, STIM1, and Homer2. These results suggest that Homer2 has a critical role in the regulatory action of SOCE activity and the interactions between CRAC channels.

• 대한의생명과학회지
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• 제18권3호
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• pp.181-192
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• 2012

Toll-like receptors (TLRs) induce innate immune responses that are essential for host defense against invading microbial pathogens. In general, TLRs have two major downstream signaling pathways; myeloid differential factor 88 (MyD88) and Toll/IL-1R domain-containing adaptor inducing IFN-${\beta}$ (TRIF) leading to the activation of NF-${\kappa}B$ and IRF3. Numerous studies demonstrated that certain phytochemicals possessing anti-inflammatory effects inhibit NF-${\kappa}B$ activation induced by pro-inflammatory stimuli including lipopolysaccharide and tumor necrosis factor-${\alpha}$ ($TNF{\alpha}$). However, the direct molecular targets for such anti-inflammatory phytochemicals are not fully identified. In this paper, we will discuss about the molecular targets of phytochemicals in TLRs signaling pathways. These results present a novel anti-inflammatory mechanism of phytochemicals in TLRs signaling.

• 전자공학회논문지S
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• 제34S권7호
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• pp.9-18
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• 1997

In this paper, we implemented the communication module in the Telephone Network Access Subsystem(TNAS) of the Advanced Communications Processing System(ACPS). We defined some kinds of communication tasks and related resources like several queues which are executed in real-time operating system, and implemented the procedures for processing the user information. Through traffic modeling and simulation, the performance of the Service Processing board Assembly(SPA) is evaluated in the aspets of system utilization and buffer size. The ACPS should accommodate various public networks such as public switch telephone network, packet switchen data network, frame realy netork, and ATM network. The communications module proposed in this paper could be used inthe interface beween the SPA and the High Speed Network Adaptor of other network interface subsystems.

• 한국분무공학회지
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• 제20권2호
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• pp.76-81
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• 2015

Swirl flow has an impact on the stabilization of the flame by the recirculation flow, improvement of the combustion efficiency. The swirl flow in the gun type burner is created by the spinner which is inside the airtube that guide the combustion air. Burner has generally the combustion device composed electronic spark plug, injection nozzle, combustion device adaptor, and spinner. These inner components change the air flow behavior passing through airtube. So, this study analyzed exhaust flow characteristics of the gun type burner according to the ratio of airtube diameter. Turbulence characteristics by the spinner was mean velocity, turbulence intensity, kinetic energy, shear stress and flattness factor of the air flow of axial direction and tangential direction from the exit of the airtube.

• 한국분무공학회지
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• 제20권2호
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• pp.70-75
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• 2015

Swirl flow in the gun type burner has an impact on the stabilization of the flame, improvement of the combustion efficiency. The swirl flow is created by the spinner which is inside the airtube that guide the combustion air. Gun type burner has generally the inner devices composed electronic spark plug, injection nozzle, combustion device adaptor, and spinner. These inner components change the air flow behavior passing through airtube. So, this study conducted the measurement using by hot-wire anemometer and analyzed effect of the swirl number of spinner on the exhaust air of gun type burner. Turbulence characteristics come up in this study was mean velocity, turbulence intensity, kinetic energy, shear stress and flattness factor of the air flow with the change of the distance of axial direction and tangential direction from the exit of the airtube.