• Molecules and Cells
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• v.41 no.1
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• pp.35-44
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• 2018

Mitochondria are responsible for supplying of most of the cell's energy via oxidative phosphorylation. However, mitochondria also can be deleterious for a cell because they are the primary source of reactive oxygen species, which are generated as a byproduct of respiration. Accumulation of mitochondrial and cellular oxidative damage leads to diverse pathologies. Thus, it is important to maintain a population of healthy and functional mitochondria for normal cellular metabolism. Eukaryotes have developed defense mechanisms to cope with aberrant mitochondria. Mitochondria autophagy (known as mitophagy) is thought to be one such process that selectively sequesters dysfunctional or excess mitochondria within double-membrane autophagosomes and carries them into lysosomes/vacuoles for degradation. The power of genetics and conservation of fundamental cellular processes among eukaryotes make yeast an excellent model for understanding the general mechanisms, regulation, and function of mitophagy. In budding yeast, a mitochondrial surface protein, Atg32, serves as a mitochondrial receptor for selective autophagy that interacts with Atg11, an adaptor protein for selective types of autophagy, and Atg8, a ubiquitin-like protein localized to the isolation membrane. Atg32 is regulated transcriptionally and post-translationally to control mitophagy. Moreover, because Atg32 is a mitophagy-specific protein, analysis of its deficient mutant enables investigation of the physiological roles of mitophagy. Here, we review recent progress in the understanding of the molecular mechanisms and functional importance of mitophagy in yeast at multiple levels.

• Journal of the Optical Society of Korea
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• v.14 no.4
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• pp.431-437
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• 2010

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

• Biomedical Science Letters
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• v.8 no.1
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• pp.29-37
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• 2002

Listeria monocytogenes poses an increasing health risk, which in part is due to increasing health risk, consumption of ready-to-eat food products and the introduction of increasing numbers of food products from regions with different dietary habits. L. monocytogenes can be present in meat, shellfish, vegetables, unpasteurised milk and soft cheese and poses a risk if food containing these products is stored at refrigeration temperature and is not properly heated before consumption, as L. monocytogenes is psychrophilic. Amplified-fragment length polymorphism (AFLP) analysis is the method of genotypic techinique in which adaptor oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification. The amplified fragments are electrophoretically separated to give strain-specific band profiles. Single-enzyme approach that did not require costly equipment or reagents for the fingerprinting of strains of Listeria monocytogenes was developed. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis was used to perform species and strain identification of Salmonella, Shigella, Yersinia and E. coli. By careful selection of AFLP primers, it was possible to obtain reproducible and sensitive identification to strain level. The AFLP patterns of L. monocytogenes are divided by the kinds of specimens in which were isolated. SE-AFLP fragments can be analyzed using standard gel electrophoresis, and can be easily scored by visual inspection, due to the low complexity of the fingerprint obtained by this method. These features make SE-AFLP suitable for use in either field or laboratory applications.

• Journal of ILASS-Korea
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• v.7 no.1
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• pp.14-20
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• 2002

It is well known that the flow and spray characteristics is critical factor on the performance and emission of a direct injection diesel engine. So this study aims to investigate the interaction of flow and spray characteristics. At first, in cylinder flow distributions in swirl adaptor for 4-valve cylinder head of DI Diesel engine were investigated under steady conditions for different SCV angles mounted on the cylinder head with steady rig test and 2-D LDV. And the in-cylinder flow was quantified in terms of mean flow coefficient and swirl ratio/tumble ratio. It was found that the swirl ratio is controlled between 2.3 and 3.8. Then spray characteristics of the intermittent injection were investigated. PDA system was utilized for measurement of a droplet size and velocity. The analyses of the PDA results are carried out with Time Dividing Method. It was found that there is a correlation between the swirl flow and SMD. The droplet size and the velocity were nearly constant value with each SCV angle. And the swirl ratio is higher, SMD smaller. The swirl ratio was helpful factor to the atomization of droplet.

• Journal of Chest Surgery
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• v.25 no.12
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• pp.1399-1403
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• 1992

The evaluation of the effectivess of ongoing cardiopulmonary resucitation efforts is dependent on the commonly used methods, such as the presence of femoral or carotid artery pulsations, arterial blood gas determinations, peripheral arterial pressure and intracardiac pressure monitoring. But recent studies suggest that end-tidal carbon dioxide tension serves as a non-invasive measurement of pulmonary blood flow and therefore cardiac output under constant ventilation. A prospective clinical study was done to determine whether end-tidal carbon dioxide monitoring in open heart surgery under cardiopulmonary bypass could be used as a prognostic indicator of bypass weaning. We monitored end-tidal PCO2 values continuously during cardiopulmonary bypass in 30 patients. "Ohmeda 5210 CO-2 monitor" under infrared absorption method were incorperated into the ventilator circuit by means of a side point adaptor between endotracheal tube and ventilator tubing. 18 patients[Group I ] were res-ucitated from partial bypass followed by aorta cross clamp off and 12 patients[Group II ] from aorta cross clamp off followed by partial bypass. But there was no difference between two groups[p>0.05]. The value of end-tidal carbon dioxide tension during ventricular fibrillation or nearly arrest state was 6.6$\pm$2.9 mmHg, and at the time of spontaneous beating was 19.3$\pm$5.6 mmHg[Mean$\pm$Standard deviation], In conclusion end-tidal carbon dioxide tension monitoring provides clinically useful, continous, noninvasive and supplementary prognostic indicator during cardiopulmonary bypass weaning procedures.rocedures.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.24 no.1
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• pp.1-6
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• 1988

For the purpose of making the detection range of fish detection system more longer and computerizing the system a parametric sound source, a timer and a digitizing circuit for the Apple II computer have been studied. The parametric sound of 5 KHz generated by passing AND gate two signals from carrier signal generator of 200KHz with modulator of 5KHz. This parametric acoustic source of 5KHz difference frequency had more higher directional resolution of 10 degrees than single frequency sound of 200KHz. Peripheral interface adaptor MC 6821 was adopted for interfacing to the Apple II personal computer. The timer consisted of six decade binary coded decimal counters (74 LS 190), and the digitizing circuit consisted of a sample and hold (LF 398) and an A/D converter(ADC 0808). The timer with 10KHz clock pulse had the measuring time from 0.1msec to 100sec. This time measuring range was satisfactory for the aim of the fish finding acoustic system.

• Proceedings of the Korean Information Science Society Conference
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• 2007.10c
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• pp.90-95
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• 2007

유비쿼터스 환경에서는 서비스 사용자가 언제, 어디서나 모든 서비스를 사용할 수 있어야 한다. 즉, 디바이스나 서비스의 상태, 위치 정보 등 특성에 관계 없이 동질의 서비스를 제공해야 한다. 이를 위해 산재한 이질적인 서브네트워크의 상호운용성을 위한 다양한 분산 시스템의 네트워크 프로토콜과 디바이스간의 통신을 통합하기 위한 연구가 필요하다. 서브네트워크의 상호운용성을 위해서는 메시지 전송 및 변환 기능이 요구되며, 서브네트워크의 변화를 동적으로 감지하여 서비스 사용자에게 알려주어야 한다. 따라서 본 연구에서는 유비쿼터스 웹서비스를 지원하기 위한 Web Services on Universal Networks를 제안하였다. 제안하는 WSUN은 Universal Service Broker의 프레임워크를 통해 SOA 기반으로 접근하고 있으며 요구 사항을 정의하여 필요한 구성 요소에 대한 설계하였다. 또한, 동적인 서비스 검색을 위한 Universal Service Registry를 사용한다. 본 논문에서는 DPWS의 동작 시나리오를 통해 이질적인 서브네트워크의 상호운용성을 지원하기 위한 DPWS 어댑터를 설계한다.

• The Transactions of the Korean Institute of Power Electronics
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• v.15 no.6
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• pp.466-476
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• 2010

In this paper, for the notebook adapter with power level below approximately 60W, a novel LLC resonant converter preceded by diode rectifier with a capacitor filter is presented. Due to the switching operation of the auxiliary switching device, the proposed LLC resonant converter can deliver power with the high efficiency characteristics from the universal line(110VAC/220VAC) and load conditions. The operating schemes on the proposed converter are analyzed and described. A 60W prototype product is built, tested and verified.

• Biomedical Science Letters
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• v.9 no.2
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• Proceedings of the Korean Society of Computer Information Conference
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• 2014.07a
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• pp.427-428
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• 2014

화재발생 시 인명 안전을 위하여 초기의 화재감지가 매우 중요한 요인이다. 기존 연기감지기의 경우 일정한 조건만 맞으면 작동하기 때문에 비화재보의 우려가 높다. 특히 차량 매연도 연료가 연소되어 나오는 연기이므로 차량정체 시 트럭 등에서 발생하는 심한 매연에도 반응하여 오작동의 가능성이 높다. 이러한 기존 화재감지기의 문제점을 해결하기 위해 다양한 IR 레이저 기반 연기검출장치가 활용되고 있다. 하지만 IR 레이저 기반 연기검출장치는 100m 거리가 이격된 레이저 발광부와 수광부 구조에 따라 발광부 레이저광선의 각도변화에 따른 수광부 레이저 포인트 위치가 매우 민감하게 변화함에 따라 초기 레이저 포인트의 초점을 정확히 정합하고, 이후 보정하기 위한 고정밀 자동 초점 정합장치가 필요하다. 이에 본 논문에서는 레이저 투광부와 수광부를 분리하여 레이저 송신기, 수신기로 구성되는 고정밀 자동초점 정합장치를 설계하고자 한다.