• BMB Reports
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• v.54 no.11
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• pp.563-568
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• 2021

Cancer cells predominantly generate energy via glycolysis, even in the presence of oxygen, to support abnormal cell proliferation. Suppression of PDHA1 by PDK1 prevents the conversion of cytoplasmic pyruvate into Acetyl-CoA. Several PDK inhibitors have been identified, but their clinical applications have not been successful for unclear reasons. In this study, endogenous PDHA1 in A549 cells was silenced by the CRISPR/Cas9 system, and PDHA1^WT and PDHA1^3SD were transduced. Since PDHA1^3SD cannot be phosphorylated by PDKs, it was used to evaluate the specific activity of PDK inhibitors. This study highlights that PDHA1^WT and PDHA1^3SD A549 cells can be used as a cell-based PDK inhibitor-distinction system to examine the relationship between PDH activity and cell death by established PDK inhibitors. Leelamine, huzhangoside A and otobaphenol induced PDH activity-dependent apoptosis, whereas AZD7545, VER-246608 and DCA effectively enhanced PDHA1 activity but little toxic to cancer cells. Furthermore, the activity of phosphomimetic PDHA1 revealed the complexity of its regulation, which requires further in-depth investigation.

• Molecules and Cells
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• v.27 no.5
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• pp.547-556
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• 2009

Parathyroid hormone is the most important endocrine regulator of calcium concentration. Its N-terminal fragment (1-34) has sufficient activity for biological function. Recently, site-directed mutagenesis studies demonstrated that substitutions at several positions within shorter analogues (1-14) can enhance the bioactivity to greater than that of PTH (1-34). However, designing the optimal sequence combination is not simple due to complex combinatorial problems. In this study, support vector machines were introduced to predict the biological activity of modified PTH (1-14) analogues using mono-substituted experimental data and to analyze the key physicochemical properties at each position that correlated with bioactivity. This systematic approach can reduce the time and effort needed to obtain desirable molecules by bench experiments and provide useful information in the design of simpler activating molecules.

• Journal of Families and Better Life
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• v.19 no.1
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• pp.253-268
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• 2001

This study is designed to extent the investigation of the Kyongbuk Womens policy the area of family life and womens economic activity. Results indicated that the primary barriers of getting a job for married women was child rearing and the diffency of working information. On the family life, communication patterns were contributed to the problem of the relationship between child and parent, and spouse relationship. And also, school violence was founded the hot problem. The results underscore the importance of child rearing support system, and affirmative action in womens employment enviroment, Family Life Educator and Family Counselor.

• Research in Community and Public Health Nursing
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• v.28 no.4
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• pp.410-420
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• 2017

Purpose: This study was to identify factors explaining health empowerment of older adults with high-risk of cardio-cerebrovascular diseases. Methods: Using a structured questionnaire, data were collected from 148 older adults residing in two areas who have one or more of the following diseases; hypertension, diabetes mellitus, hyperlipidemia, and atherosclerosis. Data were analyzed using descriptive statistics, independent t-test, analysis of variance (ANOVA), Pearson correlation analysis, and hierarchical multiple regression. Results: Health empowerment had a positive correlation with health literacy and social support. Perceived health status and social activity participation showed significant contribution in explaining health empowerment. Conclusion: This study showed that enhancing perceived health status and social activity participation are vital in an effort to improve health empowerment of the population. Main findings of this study can contribute to developing health empowerment interventions to improve health behavior practices of the older adults.

• Journal of Families and Better Life
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• v.28 no.4
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• pp.103-115
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• 2010

This study approached the leisure with the step of life cycle, analyzing the process of leisure career and leisure development in individual life. The leisure life cycle leads the other related studies by examining the concepts of continuity, change, leisure socialization, and leisure career. It also analyzes the patterns and the structures of the use of life time, using '2004 Time Use Survey' from the Korea National Statistical Office. As a result, this study suggests new 3 steps of leisure life cycle: participating and expanding stages, selective and concentrating stage, and continuing and absorbed stage. These steps set up the goal of leisure program by the periods. They also can help setting the direction of leisure activities, and can be used as basic data that can make up the leisure activity program models step by step. Moreover, the development of model can be used as valuable information to draw up the plans that can prepare for aging society and demand the support of policy.

• Journal of Plant Biology
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• v.37 no.2
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• pp.159-166
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• 1994

Direct current (DC) applied to cotyledons during a 16 h inductive dark period inhibited the flowering in the short-day plant, Pharbitis nil Choisy cv. Violet. The inhibitory effect of DC was more profound when the current flowed from roots to cotyledons, showing its polarity-dependent action. The second half on the inductive dark period was more sensitive to DC stimulus. The flowering was significantly depressed only when DC stimuli were applied to the translocation path of the floral stimulus from the induced cotyledon to the apex, suggesting that the transport of floral stimulus was damaged by the DC treatment. The vegetative apex culture bioassay system showed that a significant level of the floral stimulus activity existed in the phloem exudate from the cotyledons which would fail to form their own floral buds. These results strongly support the hypothesis that DC partially impede, at least temporarily, the transmission path of the floral stimulus from florally-induced cotyledon to the apex, rather than depressing in situ synthesis of the floral stimulus.

• Journal of Agricultural Extension & Community Development
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• v.15 no.3
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• pp.417-432
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• 2008

The purpose of this study were to discuss on the importance of community youth development programs and review the enhancement of competence in youth The research methods used the extensive review of related literatures. Based on the major results, the implications and suggestions were as follows. First, community youth activity will need to provide the opportunities of enhancing the competencies and a full complement of positive connections to youth programs. Second, communities promote to engage youth as partners with adults in the process of positive youth development. Third, youth development organizations operate to support the programs to prevent risky-behaviors or treat specific problems in terms of community youth contexts. Finally, collaboration and network are necessarily required among youth, adults, family, school, organizations within the community. Changes to work and workplace are so significant that policies refer to 'the knowledge society' at the same time 'information society'. Future workers will need to have the skills of information and communications management and control.

• Advances in Traditional Medicine
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• v.8 no.2
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• pp.187-191
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• 2008

Trema orientalis Linn. is commonly grown in many parts of Bangladesh. Its leaves have been used for analgesic and anti-diarrhoeal activity in traditional medicine. This study evaluates the potential analgesic and anti-diarrhoeal activity of methanol and aqueous extracts of leaves in experimental acetic acid induced writhing and castor oil induced diarrhoea in mice. The aqueous extract of leaves showed significant (P < 0.001) analgesic effect in acetic acid induced writhing in mice at a dose of 500 mg/kg body weight In castor oil induced antidiarrhoeal screening both extract increased latent period (P < 0.025) and decrease the number of stool (P < 0.025) at the dose of 500 mg/kg body weight comparable with that of the standard drug loperamide. The results provide a support for the use of this plant in traditional medicine and suggest its further investigation.

• School Mathematics
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• v.3 no.2
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• pp.447-473
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• 2001

Since the greater part of mathematical concepts have been developed in the direction of “from the concrete and realistic aspects to the abstract level”, children should be secured to learn mathematics genetically with various manipulative materials. The aim of this study is to instigate the active use of geoboards in mathematics classroom. To achieve this arm, we first embodied the several significances on the use of geoboards in mathematics instruction. And we then performed an instruction that children discover and justify the formula related to the area of trapezoid by exploring with geoboards, and analyzed the instructional episode to support our assertion about some secure merit accompanied by using geoboards. From this study, we obtained the conclusion that geoboard activity contains many significances such as children can explore congruence, symmetry, similarity, fundamental properties of figures, and pattern. Futhermore, geoboard activity enable children to transform a figure into other equivalently, develop spatial sense, have basic experiences for coordinate geometry, build a concrete model to explain abstract ideas, and foster the ability of problem solving and mathematical thinking.

• Journal of the Korean Applied Science and Technology
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• v.27 no.1
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• pp.37-43
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• 2010

The objective of this study was to evaluate the antifungal properties of xanthorrhizol (extract from curcuma xanthorrhiza) against Candida albicans. Some of the commercial products were evaluated for their ability to inhibit growth of C. albicans. OTC product containing povidone iodide and cosmetic grade product with surfactants were tested. Antifungal activity was shown in 1.56% of OTC product, however, there was no antifungal effect in cosmetic product. For the comparison, we tested several materials. Povidone iodide, lemon tea tree oil and xanthorrhizol showed antifungal activities against C albicans in 0.25, 0.062 and 0.007%, respectively. We also tested the antifungal effects of povidone iodide and xanthorrhizol in surfactant base. Test results revealed that 2.5% of povidone iodide and 0.156% of xanthorrhizol showed similar antifungal effects. These findings support the application of xanthorrhizol for vaginal cleanser or personal cares using antifungal effect.