• 한국미생물·생명공학회지
• /
• 제16권6호
• /
• pp.468-475
• /
• 1988

고초균 (Bacillus subtilis)의 cytidine/2'deoxycytidine deaminase (EC 3.5.4.5)를 로드하는 cdd 유전자를 cdd 결손변이주 B. subtilis ED4O에서 발현시켰다. 이 cdd 유전자는 Bacillus의 λD69 유전자은행으로부터 처음 클로닝된 것으로서 B. subtilis-Escherichia coli 의 shuttle vector pGB 215-110ΔB의 EcoRl/Pvul 부위에 삽입시켰다. 형질전환된 ED4O는 야생주에 비해 3700unit의 강한 cdd 활성을 나타내었으며 이 클론된 백터 pSO100 을 E. coli에서 발현시키면 B. subtilis 비해 2배의 강한 활성을 나타내었다. 겔 여과로 부분정제한 본 효소의 Km치는 1.88$\times$$10^{-4}$M이었으며 Vmax=11.1 $\mu$mol/min/mg 단백이었다. 이 효소는 0.1M mercaptoethanol과 수은에 의해 완전저해되었으며 p-chloromercurybenzoic acid에 대해 Ki=5$\mu$M로 나타났다. 본 효소의 활성상실은 monomer에 함유된 6개의 cysteine 잔기의 일부가 활성단으로 작용하는 과정이 저해되었거나 tetramer로서의 회합과정이 저해되었기 때문인 것으로 추측되었다.

• 드라이브 ㆍ 컨트롤
• /
• 제12권4호
• /
• pp.60-70
• /
• 2015

A front-end loader (FEL) mounted on an agricultural tractor is one of the most commonly used implements for farm work. However, when the tractor carries material using the bucket attached to the FEL on a sloping ground, the materials can spill or roll back over the operator due to the tilted body, thereby requiring the bucket surface to remain level at a constant value regardless of varying slopes. In this study, an active system for controlling the angle of the FEL bucket on a tractor based on the real-time measurement of ground slopes was developed to enable the bucket to constantly remain level. A FEL simulator operated based on an electro hydraulic proportional valve (EHPV) was constructed in the laboratory to develop a proportional-integral-derivative (PID) controller forming a virtual electronic control unit (ECU) on the computer, which could automatically adjust the bucket angles depending on varying input angles while sending SAE-J1939 associated messages via CAN BUS to the EHPV. The different parameter values for the PID controller due to the gravity effect of the bucket were determined using a manual PID tuning method while assuming that the tractor travels on either an ascending slope or a descending slope. The developed PID control-based self-leveling system showed a mean of steady-state errors of within $1^{\circ}$ and a mean of delayed times of ~ 0.8s when the step input of $+20^{\circ}$ was given, implying that the developed system and control algorithm would be effective in maintaining the bucket angle at a certain value. Future studies include the improvement of the control algorithm to reduce such a time delay as well as the application of the developed algorithm to the FEL mounted on a tractor tested at a testing ground.

• 한국습지학회지
• /
• 제6권3호
• /
• pp.83-93
• /
• 2004

본 연구의 목적은 하천환경의 정량적 평가기법을 통해 하천 관리 전략의 틀을 개발하는데 있다. 연구 대상지는 경남 진주시를 관통하여 남강으로 유입되는 6개의 지방 하천이다. 하천환경 평가는 세 가지의 현장 조사를 통해 수행되었다. 첫째, 대상 하천의 제방선으로부터 좌우 50m, 길이 500m 간격을 단위구간으로 하여 20개의 평가항목에 대해서 하천 자연도를 조사하여 5등급으로 평가하였다. 둘째, 수질 및 유량을 8회 조사하여 5등급으로 평가하였다. 셋째, 해당 하천 유역의 토지이용도를 주거지 비율을 따라 5등급으로 평가하였다. 이상의 하천환경의 세 가지 부문인자인 자연도, 수질 그리고 토지이용도를 통합하여 다시 5등급으로 분류하여 최종적인 하천 환경을 평가하였으며 그 결과에 따라 각 하천별 관리 전략을 제시하였다. 하천별 적용 결과를 살펴보면, 판문천과 가좌천은 적극적인 환경복원이 필요한 IV등급으로 평가되었고, 나불천과 용아천은 부분적 복원이 필요한 II등급으로 평가되었으며, 영천강과 독산천은 현 상태 유지가 바람직한 I등급으로 평가되었다. 이상의 대상 하천별 환경 평가는 향후 하천관리 계획수립이나 하천 복원을 위해 중요한 참고자료가 될 것으로 기대된다.

• 한국해양환경ㆍ에너지학회지
• /
• 제14권2호
• /
• pp.81-92
• /
• 2011

부산 수영만의 퇴적환경을 파악하기 위해 고해상도 탄성파 자료(chirp and sparker system)를 활용한 해석을 실시하였다. 연구해역의 퇴적단위는 음향기반을 피복하는 세 개의 퇴적단위(SU1a, SU1b, SU2)로 구성되며 각각의 퇴적단위는 침식 부정합면에 의해 구분된다. 최하부의 SU1a는 음향학적으로 평행하고 연속적인 내부 반사면의 특징을 가지며, 상부의 SU1b는 불규칙한 내부 반사면을 보인다. 최상부 퇴적단위인 SU2는 탄성파 단면상에서 투명 음향상 특징을 가진다. 음향기반 상부경계면은 침식 부정합면의 특징을 보이며 동쪽으로 향하면서 깊어지는데, 이는 인접한 수영강의 영향에 의한 것으로 생각된다. 이 침식면 상부에 퇴적된 SU1a와 SU1b는 저지대를 충진하는 형태로 분포한다. SU2는 연구해역 전반에 걸쳐 광역적으로 나타난다. 본 연구에 활용된 고해상도 탄성파 자료는 수영만의 퇴적환경 및 역사에 대한 중요한 정보를 제공하였으며, 그 결과 해수면 변동, 염하구 환경 및 인접 하천에서의 육상기원 퇴적물 유입 등이 연구해역의 주요 퇴적기작에 해당하는 것으로 해석된다.

• 공업화학
• /
• 제10권6호
• /
• pp.857-862
• /
• 1999

아민화된 poly(ether sulfone)(APES)는 poly(ether sulfone)(PES)를 질산과 황산(촉매)의 혼산으로 니트로화한 poly(ether sulfone)(NPES)를 아민화하여 제조되었다. FT-IR의 분석 결과 1537과 $1351cm^{-1}$에서 $NO_2$의 비대칭 신축진동과 대칭 신축진동에 의한 흡수피크로 니트로화가 되었음이 확인되었다. 또한 NPES의 아민화는 $NO_2$에 의한 흡수피크가 사라지고 3470과 $3374cm^{-1}$에서 $NH_2$기에 의한 비대칭 신축진동과 대칭 신축진동의 흡수피크로 확인되었다. PES(5 g; 21.55 mmol.)에 대한 니트로화의 최적 조건은 반응시간 12시간, 반응온도 $120^{\circ}C$, 질산의 농도 28.00 mmol. 황산의 농도 52.00 mmol. 이었다. APES의 원소분석결과 반복단위당 0.89개의 아민기가 도입되었다. 실리카겔이나 활성탄소보다 흡착속도는 느리나 NO에 대한 흡착능이 우수하였고, 화학적인 흡착속도는 물리적 흡착속도보다 느리며, 화학적 흡착량이 물리적 흡착량 보다 높은 것을 알 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제32권2호
• /
• pp.142-150
• /
• 2006

PURPOSE : The 3rd molar extraction of mandible is common in out-patient office of oral and maxillofacial surgery. And it is dynamic minor operation with changes of vital signs. most of patients are already sensitive about their dental treatment. The changes of emotion are reached to the highest level when patients is laid down to be treated on unit chair. It can be induced to undesirable accidents as to this fear. The undesirable complications are nausea, vomiting, hyperventilation, dyspnea, syncope, shock and so on. The severe changes of vital signs may influence their behavior and make serious medical malpractice or suit such as fracture of dental instruments and injury of proximal area. METHOD AND PATIENTS : A total of 99 selected normal patients were reviewed. Among this, 70 patients(43 men, 27 women with statistical significance) were included in this study. Each steps(pre-anesthesis, 5 minutes after anesthesis, just after mucogingival incision, just after tooth section, just after suture and gauze biting) were investigated for a change of a vital signs. It is analyzed to 2 categories,"Means" and "Tendency". The "Means" is the amount of vital signs changed in comparison with pre-step during operation. That means is the amount of vital changes by each step operation. Next, " Tendency" is changes of vital signs in comparison with step1 during operation. RESULT : This is the changing tendency of vital signs with time. That is active effect of fear and pain. Thus this "Means" and "Tendency" will present a sudden changes of vital signs and it can lead to more safe treatment. CONCLUSION : Thus, the purpose of this study is, through careful operation in each step, to less on patients' complication and increase trust between patient and OMFS. This study is a first article shown with the amount of "Means" and "Tendency" in vital signs, when a third molar of mandible is extracted. This study will be base study of patients with general diseases, because it selected only patients without general diseases.

• 한국응용곤충학회지
• /
• 제29권1호
• /
• pp.31-42
• /
• 1990

벼, 현미 및 백미를 실내에 저장하면서 저곡생태계의 구성, 군집의 천이 및 이에 따르는 군집의 에너지 변환 양상을 1987년 2월부터 1988년 11월까지 조사하였다. 곡물의 온도 변화는 약 4주간의 시차를 두고 저장실의 온도 변화에 따랐다. 벼, 현미, 백미의 함수량은 실내 상대습도가 39-90% 의 범위에서 변화하였음에도 각각 12.7 $\pm$ 0.4, 13.1 $\pm$ 0.4, 및 13.5 $\pm$ 0.3%로 안정된 수준에 있었다. 마다발동물의 군집천이는 벼, 현미, 백미에서 각각 다르게 나타났다. 벼의 경우 우점종이 가루민다드미벌레에서 어물다드미벌레로 천이하였다. 현미 및 백미의 정우에는 밀가루줄명나방에서 쌀바구미, 미동정 기생충, Anisopteromalus calandrae Howard와 거짓쌀도둑의 우점으로, 다시 쌀바구미로 천이하였다. 그러나 백미에서는 호미와는 달리 다드미벌레목 2종의 일시적인 우점 현상이 관찰되었다. 대체적으로 벼에서의 군집은 현미냐 백미에서의 군집에 비해 단순하게 구성되었으며 불안정하였다. 천립중의 약화는 벼와 백미의 경우에는 관찰할 수 없었으나 현미의 경우 마디발동물의 활동이 활발했던 시기에 다소 감소하였다. 그러나 회분함량은 현미, 백미 및 벼에서 공히 증가한 자취를 불 수 없어 저장기간 중 곡물생태계의 에너지의 손실은 미미하였단 것으로 판단되었다.

• Journal of Ginseng Research
• /
• 제34권4호
• /
• pp.314-320
• /
• 2010

The previous reports have showed that ginseng saponins, which are the active ingredients of Panax ginseng, cause the relaxation of artery that are contracted due to a various of hormones or potassium ($K^+$). Recently, we also showed that ginsenosides differentially regulate channel activity. The purpose of this study was to examine whether ginseng saponins affect contraction induced by $K^+$, serotonin (5-HT), or acetylcholine (Ach) in porcine coronary vessel. Treatment with concentrations of ginseng saponins caused a relaxation of 25 mM KCl-induced porcine coronary artery contraction. Also, ginseng saponin induced a significant dose-dependent relaxation of $3\;{\mu}M$ 5-HT-induced porcine coronary artery with the endothelium. In the porcine artery with the endothelium, ginseng saponins induced a relaxation by $3\;{\mu}M$ 5-HT in a concentration-dependent pattern. Ginseng saponins induced relaxation of both 25 mM KCl- and $3\;{\mu}M$ 5-HT-induced coronary artery contraction in the absence and presence of the endothelium. In contrast, treatment with $100\;{\mu}g/mL$ ginseng saponin did not induce relaxation in coronary artery contraction induced by Ach ($0.01\;{\mu}M$ to $30\;{\mu}M$) in the presence of the endothelium, but did cause significant relaxation of coronary artery contractions by Ach ($0.01\;{\mu}M$ to $30\;{\mu}M$) in the absence of the endothelium. These findings indicate that ginseng saponin (> $100\;{\mu}g/mL$) significantly inhibits porcine coronary artery contractions caused by $K^+$, 5-HT, and Ach. Therefore, in this study, we demonstrated that ginseng saponin may show beneficial roles on abnormal coronary contraction.

• Journal of Ginseng Research
• /
• 제44권4호
• /
• pp.570-579
• /
• 2020

Background: Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides. But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated. Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng. Methods: A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100. Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity. The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis. Results: Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α. The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis. RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%). RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan. Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I). Conclusion: we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 1999년도 The 6th International Symposium on the Development of Anti-Cancer Resource from Plants
• /
• pp.46-57
• /
• 1999

Ginseng(Panax ginseng C.A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng callus and hairy roots grow vigorously and may Produce the same or more biologically active compounds for human health than natural ginseng roots. Therefore, ginseng callus and hairy roots can be used for commercial purposes. Polyacetylene, one of anti-cancer compounds in ginseng, was not detected in the callus cultured on the medium containing 2, 4-B, but cells derived from the callus growth was excellent, The ginseng calli cultured on the medium containing 2mg11 CPA and 0.05mg/1 BA was grown vigorously and produced panaxydol, one of ginseng polyacetylene. The biosynthesis of polyacetylene in callus was not affected by addition of NAA and sucrose in media. The SH medium was better than the MS medium for ginseng callus growth and biosynthesis of panaxydol. Another ginseng anti-cancer compounds, ginsenoside-Rg$_3$, Rh$_1$and Rh$_2$ were detected in ginseng hairy roots by heat treatment. Those of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes Rl000 $A_4$T in dark condition after one month of culture. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark(22$^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5 ι Erlenmeyer flasks, 1ι roller drums, 10ι jar-fermenters, and especially in 20ι air-lift .culture vessels. All heat treatments had remarkably different ginsenoside contents. Eleven ginsenosides were determined in heat treatment, eight in freeze dried hairy roots. Contents of ginsenoside-Rbl , Rb2, Rc, Rd. Re, Rf, and Rg$_1$tested in all heat treatments were less than those of freeze dried hairy roots. Contents of glnsenoside-Rg$_2$ in heat treatment for 1 hour at 105$^{\circ}C$ was 4.92mg/g dry wt, 3.9 times higher than 1.27 mg/g dry wt of freeze dried hairy roots. The optimum condition of heat treatment for the production of ginsenoside-Rg$_3$and Rhl was 2 hours at 105$^{\circ}C$, and ginsenoside content was 2.58mg/g dry wt and 3.62mg/g dry wt, respectively. The production of ginsenoside-Rh2 was the highest in heat treatment for 2 hours at 105$^{\circ}C$ among treatments examined, and ginsenoside-Rh$_2$content was 1.08mg/g dry wt.