• The Korean Journal of Pharmacology
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• v.20 no.1 s.34
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• pp.23-32
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• 1984

The effects of morphine on the transmembrane potential and the short circuit current in the isolated frog skin were studied under different experimental conditions. The measurem ents of the transmembrane potential and the short circuit current were carried out according to Ussing and Zerahn's method. Experimental results were summerized as follows: 1) $5{\pm}10^{-3}$M of morphine markedly depressed the transmembrane potential and the short circuit current of the naive preparation. The peak of these inhibitory effects of morphine was observed about 1 hour after administration of the drug. 2) However $10^{-4}$M of naloxone did not affect these effects of morphine. 3) Decrease of $K^+$, increase of $K^+$ or $Ca^{2+}$ in the perfusate, markedly potentiated the inhibitory action of morphine on both transmembrane potential and short circuit current of the frog skin, and addition of $Mn^{2+}$ to the solution depressed the effect of morphine on the transmembrane potential, while the inhibitory effect of morphine on the short circuit current was diminished in the $Ca^{2+}$-free ringer solution, and increase of $Mg^{2+}$ concentration depressed those effect of morphine on both electrical parameters. 4) In the morphine treated preparations, transmembrane potential and short circuit current were decreased in the early phase of drug treatment ($1{\sim}2$ days), but gradually increased to the significantly high level from the control (48 days after treatment). In these preparations, the effects of morphine on both electrical parameters were also potentiated in the early phase, but markedly diminished in the late phase of treatment. From the above results, it is postulated that the pharmacological actions of morphine as well as development of the tolerance by morphine may be partially related to the changes of ion fluxes and/or permeabilities of skin by the drug.

• Korean Journal of Health Education and Promotion
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• v.22 no.2
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• pp.155-171
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• 2005

Objectives: The purpose of this study is to find out how well the primary school students are practicing the health promotion behaviors and the factors relating their health promotion behavior. Methods: This study was carried out by using questionnaires with 1,215 students attending at a 6th grade, 16 primary schools which arranged the school health teachers in Kyoungpook, Chilkok Gun from the 21th through 22th of December 2004. Among them, 1,189 were included in final analysis. The collected data was analyzed through frequency, percentage mean and standard deviation, multiple regression. Results: According to the data from multiple regression of analysis which has the health promotion behavior practice as a subordinate variable, in male students' case the degree of health promotion behavior practice becomes high in proportion to their parents' interest in health, to their perceived self efficacy, and in case of the barriers of the health promotion behavior practice. As to the female students, their health behavior practice becomes high in proportion to their parents' interest in health. It also becomes high in proportion to the perceived health status, the understanding of the importance of the health, internal health locus of control in personality, the perceived self efficacy and the perceived benefit of the health promotion behavior. But in case of the barriers of the health promotion behavior, it was the same as the male students' case. Conclusions: As the results of study, it showed that the knowledge to importance of health, the self efficacy, the knowledge of benefit of health promotion behaviors and the health concern to the children of the parents were related to health promotion behaviors of the primary school students. So, through the systematic development of health education program, we induced to desirable direction for the changeable factors of actions to health promotion for the health, and through the connective guidelines between the School and the Home, we have to adapt to effective health promotion program for the health management of the young boys and girls.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.26 no.1
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• pp.19-34
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• 2013

Objective : This study was performed to evaluate the effect of immune reaction inductive substances such as PMA, LPS, DPE, DNCB and WP, the whitman pigra extracting substance at simultaneously on the translocation of $NF{\kappa}B$ towards to the nucleus and the mRNA expression patterns of various cytokine genes in THP-1 cells, monocytes of human. Methods : To analyze the cytokine genes expressions, the RT-PCR method was used, and measuring TNF-${\alpha}$ that had been secreted during cell culture by the ELISA method. The morphological changes were observed during THP-1 cell by a scanning electron microscope and the quantitative distribution of $NF{\kappa}B$ in the cell that was analyzed through immunocytochemistry and a confocal microscopy. Results : WP showed different influences onto the mRNA expression patterns of cytokine genes with PMA, LPS. DPE and DNCB according to the types of immune inductive substances in the THP-1 cells. Upon treating PMA and DPE on the THP-1 cells at the same time or either additionally treating WP thereon, the movement of $NF{\kappa}B$ increase towards the nucleus from cell cytoplasm was able to be observed. The expressions of IL-$1{\alpha}$ and IFN-${\gamma}$ induced by PMA and PMA+DNCB were suppressed by WP while the expression of TGF-${\beta}$ was promoted. Regarding the secretion pattern of TNF-${\alpha}$ according to the treatment of PMA, its secretion amount was incredibly increased by concurrent treatment of WP, however, in case of co-treatment of WP with PMA and DNCB, it was found that the secretion amount of TNF-${\alpha}$ decreased. Conclusions : In this study, the WP extracting substance was confirmed that it had an influence on expression patterns of cytokine genes according to the actions of a variety kinds of immune reaction inductive substances treated on the THP-1 cells. Especially, WP co-treatment with PMA and DNCB was suppressed the expression of inflammatory cytokines, such as IL-$1{\alpha}$, IFN-${\gamma}$ and TNF-${\alpha}$.

• The Korean Journal of Physiology and Pharmacology
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• v.5 no.4
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• pp.343-351
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• 2001

This study was undertaken to investigate the mechanism of lipopolysaccharide (LPS) and nitric oxide (NO) as a regulator of vascular smooth muscle cell (VSMC) proliferation. VSMC was primarily cultured from rat aorta and confirmed by the immunocytochemistry with anti-smooth muscle myosin antibody. The number of viable VSMCs were counted, and lactate dehydrogenase (LDH) activity was measured to assess the degree of cell death. Concentrations of nitrite in the culture medium were measured as an indicator of NO production. LPS was introduced into the medium to induce the inducible nitric oxide synthase (iNOS) in VSMC, and Western blot for iNOS protein and RT-PCR for iNOS mRNA were performed to confirm the presence of iNOS. Inhibitors of iNOS and soluble guanylate cyclase (sGC), sodium nitroprusside (SNP) and L-arginine were employed to observe the action of LPS on the iNOS-NO-cGMP signalling pathway. LPS and SNP decreased number of VSMCs and increased the nitrite concentration in the culture medium, but there was no significant change in LDH activity. A cell permeable cGMP derivative, 8-Bromo-cGMP, decreased the number of VSMCs with no significant change in LDH activity. L-arginine, an NO substrate, alone tended to reduce cell count without affecting nitrite concentration or LDH level. Aminoguanidine, an iNOS specific inhibitor, inhibited LPS-induced reduction of cell numbers and reduced the nitrite concentration in the culture medium. LY 83583, a guanylate cyclase inhibitor, suppressed the inhibitory actions of LPS and SNP on VSMC proliferation. LPS increased amounts of iNOS protein and iNOS mRNA in a concentration-dependent manner. These results suggest that LPS inhibits the VSMC proliferation via production of NO by inducing iNOS gene expression. The cGMP which is produced by subsequent activation of guanylate cyclase would be a major mediator in the inhibitory action of iNOS-NO signalling on VSMC proliferation.

• Journal of the Ergonomics Society of Korea
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• v.32 no.6
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• pp.481-487
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• 2013

Objective: The present study aims to evaluate the visual reactions of users when they are playing games of different flow levels, and to explore the visual variables that can sensitively reflect the different flow levels. Background: The flow is defined as a psychological state where interface users feel their actions in a virtual setting identical to those in real environment. To measure the flow states of users, the questionnaire-based FSS(Flow State Scale) has mostly been used. However, this method is a qualitative test that has limits in terms of the accuracy of users' flow experiences. Therefore, more accurate methods to measure users' flow experiences are required. Method: Ten subjects participated in the experiment, where the independent variables were three games with different flow levels(puzzle games, dot drawing and coloring) and the time frame(the first and last 10 seconds in game playing), whereas the dependent variables included the pupil size and the frequency and duration of eye blinking. This study was a within-subject design. Each participant performed three types of games with different flow levels 3 times for each for 10 minutes, and their visual reactions to each game were measured. Results: The higher the flow cause the bigger pupil size(p<0.01) and the lower eye blinking frequency(p<0.1), indicating that different types of games lead to different flow levels. The pupil size during the last 10 seconds when the flow level was higher was bigger by 2.1% compared with that during the first 10 seconds in game playing(p<0.1), and the eye blinking frequency decreased by 12%(p<0.01). Conclusion: It was found out that the pupil size and the frequency and duration of eye blinking were psychophysiological indices for evaluating users' flow experiences, which could quantify the flow states users go through. The psychophysiological variables capable of measuring diverse aspects of the flow need diversifying to be applicable to precise measurement of the flow. Application: These studies are warranted for both quantitative analysis of flow levels and qualitative improvement of cyber leisure in line with development of healthy games.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.513-522
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• 2017

Rapid urbanization is being portrayed around the world au causing difficulty to manage public hygiene and maintain public order. Common problems often detected in urban areas including traffic congestion and the deterioration of the ecological environment are also being exacerbated. Despite all the effort being made by the government and local governments to manage urban facilities, urban residents are subjected to various levels of difficulty, due to the number of facilities to be managed exceeding the number of management personnel and the lack of an adequate management system. The aim of this study is to propose a management method using smart phones to improve the maintenance efficiency of urban facilities. First, the one-stop maintenance of urban facilities, including facilities information management, maintenance management and history management, is made possible by using smart phones to collect and transfer pictures and the locations of the urban facilities. Second, maintenance management is based on the location and picture information of the subject, not the location of the smart phones, which enables a prompt understanding of and actions to be taken for the facility. This method is especially effective as the smart phone application sends the facility status information directly to the maintenance personnel. Third, all of the information and figures relating to the facilities is managed using a database, resulting in the easy utilization of the history management and data. Fourth, all of the urban residents have access to this information via smart phone applications and, therefore, expanding the role of the facility maintenance personnel is made possible without any additional investment in infrastructure. Lastly, the location-based information enables the management of roads, trees and trails.

• Journal of the Economic Geographical Society of Korea
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• v.17 no.1
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• pp.1-27
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• 2014

This paper aims at exploring the multi-scalar processes through which the Gumi Industrial Complex was developed in the late 1960s and the early 1970s. Existing studies, influenced by the "Developmental State Thesis", tend to see the industrialization processes of South Korea either by focusing on the socio-politico-economic processes at the national scale or in terms of the plan rationality of the national bureaucrats. This paper, however, denies this perspective on the basis of the strategic relational approach to the state and the multi-scalar perspective. In particular, it argues that the state actions for national industrialization have been the outcome of complex interactions, conflicts and negotiations among social forces, acting in and through the state, and at diverse geographical scales. This paper attempts to empirically prove this argument on the basis of a case study on the construction processes of Gumi Industrial Complex. The development of Gumi Industrial Complex cannot be solely explained in terms of either the plan rationality of the national bureaucrats or the political motivation related to the fact that Gumi was the hometown of President Park Jung-Hee. This paper argues that the development of Gumi Industrial Complex was heavily influenced by the role of the following actors; place-dependent local actors in Gumi and the multi-scalar agents, such as the Korean-Japanese businessmen and the national parliament members elected in the Gumi electoral district.

• The Korean Journal of Physiology
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• v.6 no.1
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• pp.11-18
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• 1972

In an attempt to observe the Possible radioprotective actions of single or combined application of reduced glutathione (GSH) and cold exposure in mammals, the albino mouse was subjected to GSH injection, cold exposure at $-1{\pm}0.2C$ and whole-body X-irradiation with 900r either singularly or in combination, or the X-irradiation following the cold exposure and/or GSH injection. The levels of intrinsic NP-SH and NP-SS of the liver, brain and heart were measured at one hour after each application, and the results were compared with the control, i.e., non-irradiated and non-cold exposed normal animal. NP-SH was measured by the Ellman's method, and NP-SS was measured by the electrolytic reduction method described by Dohan and Woodward. The results thus obtained are summarized as follows: 1) The levels of NP-SH in the liver, brain and heart of the normal mouse was $6.35{\pm}0.61,\;2.65{\pm}0.15\;and\;3.17{\pm}0.10{\mu}\;mol/gm\;wet\;wt.$, respectively, and NP-SS was $3.09{\pm}0.11,\;2.95{\pm}0.20\;and\;0.18{\pm}0.24{\mu}\;mol/gm\;wet\;wt.$, respectively. 2) Though there were some degrees of difference among the tissues studied, a general tendency of (1) elevated NP-SH and NP-SS levels in the GSH injection group, (2) similar or slightly elevated NP-SH and NP-SS levels in the cold-exposed group, and (3) markedly decreased levels of NP-SH and NP-SS in the X-irradiated group, was observed. When GSH was injected prior to the X-irradiation, NP-SH and NP-SS in all the tissues studied showed generally higher values than in the group where the X-irradiation was given alone, and the values were close to the normal. In the group where the cold exposure was applied immediately after the X-irradiation, no significant difference was observed in the NP-SH and NP-S5 levels comparing with the X-irradiation group. On the contrary, when GSH was injected immediately prior to the X-irradiation or cold exposure, NP-SH and NP-SS levels were either similar to, or higher than, the normal value.

• Steel and Composite Structures
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• v.6 no.6
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• pp.459-477
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• 2006

A theoretical research project is undertaken to develop integrated analysis and design tools for long span composite beams in modern high-rise buildings, and it aims to develop non-linear finite element models for practical design of composite beams. As the first paper in the series, this paper presents the development study as well as the calibration exercise of the proposed finite element models for simply supported composite beams. Other practical issues such as continuous composite beams, the provision of web openings for passage of building services, the partial continuity offered by the connections to columns as well as the behaviour of both unprotected and protected composite beams under fires will be reported separately. In this paper, details of the finite elements and the material models for both steel and reinforced concrete are first described, and finite element studies of composite beams with full details of test data are then presented. It should be noted that in the proposed finite element models, both steel beams and concrete slabs are modelled with two dimensional plane stress elements whose widths are assigned to be equal to the widths of concrete flanges, and the flange widths and the web thicknesses of steel beams as appropriate. Moreover, each shear connector is modelled with one horizontal spring and one vertical spring to simulate its longitudinal shear and pull-out actions based on measured load-slippage curves of push-out tests of shear connectors. The numerical results are then carefully analyzed and compared with the corresponding test results in terms of load mid-span deflection curves as well as load end-slippage curves. Other deformation characteristics of the composite beams such as stress and strain distributions across the composite cross-sections as well as distributions of shear forces and slippages in shear connectors along the beam spans are also examined in details. It is shown that the numerical results of the composite beams compare well with the test data in terms of various load-deformation characteristics along the entire deformation ranges. Hence, the proposed analysis and design tools are considered to be simple and yet effective for composite beams with practical geometrical dimensions and arrangements. Structural engineers are strongly encouraged to employ the models in their practical work to exploit the full advantages offered by composite construction.

• Journal of the Korean earth science society
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• v.28 no.6
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• pp.699-706
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• 2007

The purpose of this study is to examine the perceptions of pre-service teachers, which directly affect the concepts and ways of thinking of students who are studying Earth science. This work further seeks to identify pre-service Earth science teachers' Earth science-centered ways of thinking regarding the components and the process of water cycle. The concept sketch method was used to survey 50 pre-service teachers who majored in Earth science education at a National Teachers' University. The survey analyzed the preservice teachers' perceptions of a subordinate concept of Earth systems from the applied components of water cycle, and to code the applied concepts with the components of the water cycle based on a subordinate concept of Earth systems including Hydrosphere, Atmosphere, Geosphere, and Biosphere. The preservice teachers only perceived the components of the water cycle as ones in Hydrosphere and Atmosphere. In Biosphere, many participants lack the knowledge about how the actions of plants and animals, and human beings impact the water cycle. About the process of water cycle, the majority of the participants only perceived evaporation and precipitation as the process of cycling: their understanding about the underground flow of water was more lacking than the surface flow.