• Journal of Environmental Health Sciences
• /
• v.16 no.2
• /
• pp.127-133
• /
• 1990

The purpose of the study was to identify the characteristics of the pathogenic microorganism Actinobacillus actinorayceteracomitans. The experiments of this study consist of the decision of existence or nonexistence of Actinobacillus actinorayceterncoraitans in the air of dental clinics, the effect of sugar on the growth of Actinobacillus actinorayceteracoraitans, and the sensitivity of Actinobacillus actinoraycetemcoraitans to Fluorine and antibiotics. The results of the experiments are as follows. 1. Actinobaeillus actinornyceteracornitans did not exist in the air of 4 dental clinics in Seoul 2. The growth of Actinobacillus actinornycetemcornitans in the media containing glucose or sucrose was faster than in the media without these, and glucose was more effective than sucrose. 3. Actinobacillus actinomyceteracoraitans was not susceptible to Fluorine at concentration between 0 ppm and 300 ppm. 4. Order of sensitivity of Actinobacillus actinomycetemcomitans to antobiotics was as follows: Tetracycline > Kanamycin > Ampicillin.

• Korean Journal of Microbiology
• /
• v.41 no.3
• /
• pp.183-187
• /
• 2005

This study was performed to investigate the hygiene condition of PC room(internet cafe) in Seoul Korea. From July 2004 to December, 34 samples were collected, there's an average of $9.0{\times}10^4$ CFU/ml on keyboards, $2{\times}10$ CFU/ml on mouse and $5{\times}10^3$ CFU/ml on door konbs toilets, suggesting that keyboards and mouse are more contaminated than toilet door knobs. Seven antimicrobial resistant strains were isolated from PC Rooms. Two isolates were resistant to methicillin and erythromycin, while five isolates were resistant to gentamicin, ampicllin, cefotaxim, and chloramphenicol. By identification, these strains were identified as Staphylococcus aureus (2 strains). Actinobacillus ureae (4 strains) and Pasteurella multocida (1 strain), respectively. Pasteurella multocida and Actinobacillus ureae are potentially pathogenic bacteria. Actinobacillus ureae, formerly, known as Pasteurella ureae, is an uncommon of the upper respiratory tract in humans. Pasteurella multocida is a part of the normal flora in the nasopharynx of many domestic animals. We concluded that Staphylococcus aureus is highly resistant to erythromycin and methicillin over $100\;{\mu}g/ml$, while Pasteurella multocida and Actinobacillus ureae is highly resistant to gentamicin, ampicillinover over $100\;{\mu}g/ml$.

• Journal of Life Science
• /
• v.8 no.5
• /
• pp.526-531
• /
• 1998

In this study, the composition and characteristics of poly-$\beta$-hydroxybutyrate (PHB) biosynthesized by Actinobacillus sp. EL-9 are investigated. PHB produced by Actinobacillus sp. EL-9 was identified as the homopolymer of 3-hydroxy-butyric acid (PHB) by infrared spectroscopy and nuclear magnetic resonance spectroscopy analysis. The melting tem-perature (T$_{m}$), and crystallization temperature(T$_{c}$) of PHB was 169.7$^{\circ}C$ and 69.13$^{\circ}C$, respectively. The viscosity on he basis of Brookfield viscometer was 6.01 ㎗/g. The viscosity-average molecular weight estimated by Mark-Ho-wink-Sakurada equation was 1.08$\times$10$^{6}$ ($\pm$3,000).00).

• Journal of Periodontal and Implant Science
• /
• v.26 no.2
• /
• pp.365-375
• /
• 1996

5 serotypes(a, b, c, d, e) of Actinobacillus actinomycetemcomitans showed distinct hybridization patterns(DNA fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7-Kb sized DNA probe. The sizes of hybridized bands in each serotypes were different among serotypes and represented unique patterns of hybridization with the probe used. The serotype a showed two bands of fingerprinting patterns: 23.1 kb and 2.5 kb respectively. Serotype b and c showed single band: 6.6 kb and 9.5 kb, respectively. Serotype d and e showed two bands of hybridization: 23.1 kb and 2.8 kb, and 23.7 kb and 2.1 kb, respectively. The results indicate that this standard fingerpriting patterns of DNA hybridization with 4.7 kb probe can be further used for genotyping clinical isolates of Actinobacillus 8ctinomycetemcomitansand its relevance with periodontal disease activity.

• Journal of the Korean Wood Science and Technology
• /
• v.30 no.3
• /
• pp.26-33
• /
• 2002

The seven strains, Pseudomonas paucimobilis, Pseudomonas cepacia, Staphylococcus auricularis, Staphylococcus saprophyticus, Acidovorax spp., Acinetobacter calcoaceticus, and Actinobacillus capsulatus were tested with three slimicides. Most of the tested bacteria were inhibited with slimicide K (an isothiazolin based compound), even at its low concentration, except for Actinobacillus capsulatus and Staphylococcus auricularis. Both slimicides B (an organic bromine based compound) and S (aldehydes) also couldn't prevent these two strains even at their highest concentration. Five different sizes of plasmid DNA were isolated from Actinobacillus capsulatus. Staphylococcus auricularis, a gram-positive bacteria, showed the slimy substances around its cell distinctively. The results suggest that two strains, Actinobacillus capsulatus, Staphylococcus auricularis, have presumably developed a resistance to the slimicide, by plasmid DNA or slimy substance. Our findings also suggest that not only gram-negative bacteria, but also gram-positive bacteria should not be neglected

• Journal of Periodontal and Implant Science
• /
• v.17 no.1
• /
• pp.103.1-103.1
• /
• 1987

• Journal of Periodontal and Implant Science
• /
• v.20 no.1
• /
• pp.147.1-147.1
• /
• 1990

• Journal of Periodontal and Implant Science
• /
• v.14 no.1
• /
• pp.73-73
• /
• 1984

• Microbiology and Biotechnology Letters
• /
• v.24 no.3
• /
• pp.344-351
• /
• 1996

Microorganisms growing alcoholic distillery wastes were isolated from soil. Of them, the selected strain EL-9 had a capability of accumulating poly-$\beta$-hydroxybutyrate (PHB) when grown in alcoholic distillery wastes. The strain EL-9 was identified as a genus Actinobacillus based on the morphological, cultural, and biochemical characteristics. The strain EL-9 was named temporarily as Actino- bacillus sp. EL-9. The optimal temperature and pH for cell growth of Actinobacillus sp. EL-9 were 30$\circ$C and 7.0, respectively. The optimal medium compositions for cell growth comprised glucose 2%, NH$_{4}$NO$_{3}$ 0.15%, Na$_{2}$HP0$_{4}$-12H$_{2}$O 0.25%, KH$_{2}$PO$_{4}$ 0.25%, MgSO$_{4}$4-7H$_{2}$O 0.15%, FeSO$_{4}$-7H$_{2}$O 0.005%, CaCl$_{2}$-2H$_{2}$O 0.003% and trace element solution 5m/l. To investigate the optimal conditions for PHB production, two-stage culture technique was used. The result showed that the optimal conditions for PHB production are identical those for cell growth. When propionic acid was added as a cosubstrate, PHB/HV copolymer was produced.

• Journal of Periodontal and Implant Science
• /
• v.25 no.3
• /
• pp.679-693
• /
• 1995

The present study has been performed to see the intrafamilial transmission of periodontopathic organism Actinobacillus actinomycetemcomitans in Koreans having various froms of periodontal diseases. 17 clinical isolates from 8 periodontal patients and 20m clinical isolates from their 8 family members were grown anaerobically for the serotyping and the extraction of genomic DNA. The DNA was digested with restriction endonucleases (EcoRI+HindIII) and plasmid pAA 2097(kindly provided by Dr. DiRienzo, Univ. of Pennsylvania) including 4.7kb-size randlomly clone probe for restriction length pleomorphism analysis(RFLP). RFLP patterns of reference serotypes a, b, c, d, and e were used as the genotypes A, B, C, D, and E, respectively for comparison of genotypes of clinical isolates. 28 out of total 37 clinical isoltes belonged to either one of 5 basic gentotypes and 9 remaining isolats did not fall into any types, and hence were designate as non-type(NT). Genotype C were the most frequently found one(35.1%) and genotype B has not isolated. Intrafamilial transmission of bacteria between spouses, brothers and sisters, and parents and their offsprings, resepctively could well be demonstrated by comparing RFLP patterns. There were not any specific genotypes which showed predominance over others in terms of transmission.