• Journal of Animal Science and Technology
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• 제64권5호
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• pp.950-969
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• 2022

This study aimed to determine the effect of dietary supplementation with Acremonium terricola culture (ATC) on the quality, conventional characteristics, and flavor substances of Hortobágy goose meat. A total of 720 one-day-old goslings were divided into four dietary treatments, each consisting of six cages of 30 goslings. The dietary conditions consisted of the control group and three treatment groups supplemented with 3, 5, or 7 g/kg ATC. In male geese, supplementation with 3 g/kg ATC elevated the crude ash (CA) content of the thigh muscle compared to the control group, and the CA content of the pectoralis major was significantly elevated when geese were supplemented with 5 g/kg ATC (p < 0.05). In females, compared with the control group, supplementation with 7 g/kg ATC enhanced the crude protein (CP) content of the pectoralis major. Supplementation with 7 g/kg ATC also increased the crude fat (CF) content of the pectoralis major in females as well as in both sexes; moreover, this supplementation dose increased the inosinic acid content of the thigh muscle in males and in both sexes. In contrast, supplementation with 5 g/kg ATC decreased the pH of the thigh muscle at 12 h postmortem (p < 0.01). No significant changes in meat color, water loss rate, shear force, moisture content or amino acid (AA) levels were observed after ATC supplementation (p > 0.05). Levels of saturated fatty acids (SFAs) and polyunsaturated FAs (PUFAs) in the pectoralis major and levels of SFAs, monounsaturated FAs (MUFAs), and PUFAs in the thigh muscle were not affected by the supplementation. Overall, ATC supplementation had positive effects on the pH, and CA, CP, CF, inosinic acid contents as well as on the FA composition of gosling meat. The optimal level of ATC supplementation was 7 g/kg in goslings from 1 to 70 days of age.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.165-170
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• 2023

Isolation of the culture broth of a marine-derived Acremonium sp. CNQ-049 guided by HPLC-UV yielded compound 1 (3-phenethyl-2-phenylquinazolin-4(3H)-one), and its inhibitory activities against monoamine oxidases (MAOs), cholinesterases (ChEs), and β-secretase 1 (BACE1) were evaluated. Compound 1 was an effective selective MAO-B inhibitor with an IC₅₀ value of 9.39 µM and a selectivity index (SI) value of 4.26 versus MAO-A. In addition, compound 1 showed a potent selective butyrylcholinesterase (BChE) inhibition with an IC₅₀ value of 7.99 µM and an SI value of 5.01 versus acetylcholinesterase (AChE). However, compound 1 showed weak inhibitions against MAO-A, AChE, and BACE1. The Ki value of compound 1 for MAO-B was 5.22±1.73 µM with competitive inhibition, and the Ki value of compound 1 for BChE was 3.00±1.81 µM with mixed-type inhibition. Inhibitions of MAO-B and BChE by compound 1 were recovered by dialysis experiments. These results suggest that compound 1 is a dual-functional reversible inhibitor of MAO-B and BChE, that can be used as a treatment agent for neurological disorders.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.51.1-51
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• 1999

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1120-1124
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• 2006

Supplementation with rice oil and its major components (oleic acid and linoleic acid) was found to have a significant influence on cephalosporin C (CPC) production and cell growth by A. chrysogenum M35 in shake flask cultures. Five percent (v/v) rice oil had the most robust effect and 5% (v/v) oleic acid was the second most efficient on cell growth, whereas 3% (v/v) linoleic acid was found to be optimal for CPC production. Rice oil, oleic acid, and linoleic acid also significantly improved the rates of glucose consumption. When glucose was almost consumed, CPC production was initiated and, on the addition of rice oil, lipase activity increased steadily to 1.56 U/ml for 4 days. These results suggest that rice oil and fatty acids are used as carbon source to produce CPC by A. chrysogenum M35. Moreover, a mixture, composed of 40% (v/v) oleic acid and 60% (v/v) linoleic acid, had the strongest stimulatory effect on CPC production, due to a synergistic effect of the two fatty acids. Consequently, the maximum CPC titer (7.44 g/l) was improved about 4.5-fold.

• 한국수산과학회지
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• 제50권2호
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• pp.219-221
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• 2017

In 2015, white cottony tufts were observed on gametophytes of the kelp Saccharina japonica. Wet mount and histopathology examination revealed numerous fungal hyphae and mycelium around the gametophytes. The gametophytes surrounded by fungal hyphae were generally round and empty. A specific 610-bp fragment of the internal transcribed spacer (ITS)-5.8S rDNA-ITS gene of fungi was amplified by polymerase chain reaction and the nucleotide sequence showed 100% identity with those of Acremonium sclerotigenum, Acremonium sp. and Ascomycota sp. When fungus-infected gametophytes were mixed with healthy gametophytes, a high transmission rate (100%) resulted. This is the first report of mycosis of gametophytes in Korea.

• 한국미생물·생명공학회지
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• 제20권2호
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• pp.178-182
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• 1992

Cephalosporium acremonium CSA-2.8A3 변이주로 부터 생성된 cephaloporin C를 정제하여 산업적 이용을 위해 ultrafiltration으로 균체를 제거하고 수종의 수지공정을 통하여 소디움염 형태의 cephalosporin C를 정제하였으며 역삼투막에 의한 농축과 분무건조를 행하였다. WA-30, HO-20, XAD-2000, SK-1B column chromatography를 통해 90% 이상의 높은 수율과 순도를 얻을 수 있었고, 특히 상기 수지들을 직렬로 연결하여 사용합으로써 수율은 96%까지 올릴 수 있었다.

• 한국균학회지
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• 제44권4호
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• pp.271-288
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• 2016

The present study reports 16 species of Ascomycota that were previously unknown in Korea, namely Acremonium cellulolyticus (KNU14-25), Acremonium zonatum (KNU14-15), Chaetomium madrasense (KNU14-9), Cladosporium silenes (KNU 14-18-1), Humicolopsis cephalosporioides (KNU15-3), Leptosphaerulina chartarum (KNU14-16), Paecilomyces marquandii (KNU14-8), Paecilomyces tenuis (KNU14-18-2), Paraphaeosphaeria sporulosa (KNU15-2), Penicillium rubidurum (KNU14-12), Pochonia suchlasporia (KNU15-6), Sporothrix inflata (KNU15-8), Thielavia hyrcaniae (KNU15-1), Thielavia terricola (KNU14-23-1), Xylogone sphaerospora (KNU15-7), and Zopfiella longicaudata (KNU15-5). These fungal species were isolated from soil samples collected from various regions of Korea and identified based on their morphological characteristics and rDNA internal transcribed spacer sequence data. Full descriptions and illustrations for each species are provided.

• 한국균학회지
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• 제49권4호
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• pp.477-486
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• 2021

A survey of insect-associated fungi in Korea revealed a novel fungal strain isolated from the bean bug Riptortus clavatus (Heteroptera: Alydidae). Culturally and morphologically, the fungal strain designated KNUF-20-INY03, shares features with members of the genus Acaulium. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer regions (ITS) regions and partial sequences of the translation elongation factor 1-alpha (TEF1-α), and β-tubulin (β-TUB), and large subunit of the nuclear ribosomal RNA (LSU) genes showed that the isolate is part of a clade that includes other Acaulium species, but it occupies a distinct phylogenetic position. Based on the shape, size, and color of its conidia and conidiogenous cells, strain KNUF-20-INY03 is readily distinguishable from the closely related A. acremonium, A. albonigrescens, A. caviariformis, A. pannemaniae, and A. retardatum. The conidial length-to-width ratio (1.6) of the novel isolate is significantly lower than that of A. acremonium (1.9), A. albonigrescens (2.4), and A. pannemaniae (2.4), and KNUF-20-INY03 produces hyaline conidia and elliptical conidiogenous cells while A. caviariformis forms brown conidia and A. retardatum produces flask-shaped conidiogenous cells. Thus, both phylogenetic and morphological analyses indicate that this strain is a novel species in the genus Acaulium, and we propose the name Acaulium microspora sp. nov.

• Mycobiology
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• 제47권1호
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• pp.50-58
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• 2019

Agarum clathratum, a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying A. clathratum was investigated and related ${\beta}$-glucosidase and endoglucanase activities were described. A total of 233 fungal strains were isolated from A. clathratum at 15 sites and identified 89 species based on morphology and a multigene analysis using the internal transcribed spacer region (ITS) and protein-coding genes including actin (act), ${\beta}$-tubulin (benA), calmodulin (CaM), and translation elongation factor (tef1). Acremonium, Corollospora, and Penicillium were the dominant genera, and Acremonium fuci and Corollospora gracilis were the dominant species. Fifty-one species exhibited cellulase activity, with A. fuci, Alfaria terrestris, Hypoxylon perforatum, P. madriti, and Pleosporales sp. Five showing the highest enzyme activities. Further enzyme quantification confirmed that these species had higher cellulase activity than P. crysogenum, a fungal species described in previous studies. This study lays the groundwork for bioremediation using fungi to remove decaying seaweed from populated areas and provides important background for potential industrial applications of environmentally friendly processes.

• 헤리티지:역사와 과학
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• 제14권
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• pp.225-250
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• 1981

경남지방(慶南地方)의 지류(紙類) 및 섬유질유물(纖維質遺物) 보관고내(保管庫內) 공중균(空中菌)의 분포(分布) 조사(調査)를 위하여 중요민속자료(重要民俗資料) 37호(號) 이휴정소장출토복식(二休亭所藏出土服飾) 등(等) 4개소(個所)를 대상으로 하였다. 분리배지(分離培地)는 malt extract agar이었으며 잡균(雜菌)의 증식(增殖)을 방지(防止)하기 위(爲)하여 chloram phenicol을 첨가하여 사용하였다. 4개소 유물보관고(遺物保管庫)에서 분리(分離) 동정(同定)된 공중(空中) 사상균(絲狀菌)은 Cladosprium cladosprioides, Alternaria chlamydo-spora, Aspergillus fumigatus, A. versicolor, Eurotium chevalieri penicillum charlesii Var. rapidum, P. oxalicum, P. viridcatum, Trichoderma viride, Acremonium sp, Mucor sp, yeast 등(等) 8속(屬) 9종(種) 이었으며 또한 4개소(個所)의 유물보관고내(遺物保管庫內)에 분포(分布)된 사상균(絲狀菌) 포자(包子)는 Cladosporium cladosporioides가 제일 많았고 그 다음이 Acremonium sp, penicillium, Aspergillus Trichoderma, Aeternaria, Eurotium의 순서이었다. 또한 지류(紙類) 및 섬유질문화재(纖維質文化財)로부터 사상균(絲狀菌)을 직접 채취(直接 採取)하여 분리(分離) 동정(同定)한 결과(結果) 8속(屬) 14(種)이 검출되었다. 이중에서 가장 많이 분리(分離)된 종(種)은 Clodosporium cladosporioides이었고 그 외에 Acremonium, Penicillium, Asper gillus, Trichoderma 등도 분리(分離) 동정(同定) 되었다. 이중에서 특히 아직 우리 나라에서 미기록종(未記錄種)은 Chacetomium globosum, Mucor racemosus, Pspinalosum, Torulaellisii 등(等) 4종(種)이 발견(發見)되었음을 확인(確認)하였다. 끝으로 화학약제(化學藥劑)에 대(對)한 방균(防菌) 효과(效果) 실험(實驗)은 Aspergillus penicillium, cladosporium Trichoderma 등(等) 8종(種)을 대상으로 하였으며 약품(藥品)은 Benzoic acid, Sorbic acide, Dehydroacetic acid, Thymol 등(等) 4가지 화학약품(化學藥品)을 사용(使用)하여 방균효과(防菌效果)를 조사(調査) 하였다. 동실험결과(同實驗結果) Thymol은 0.5%이상(以上)에서 모든 균(菌)의 생장(生長)을 저지하였으며 기타 3가지의 화학약제(化學藥劑)는 각각(各各)의 농도(濃度)에 따라 억제상황(狀況)이 상이(相異)하게 나타났음을 보고(報告) 드린다.