• Title/Summary/Keyword: Acid Red 14

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Inhibitory Effects of Ethanol Extract of Red Sweet Pepper (Capsicum annuum L.) on Triglyceride Biosynthesis in Rhodosporidium toruloides (Rhodosporidium toruloides에서의 적색 파프리카 에탄올 추출물의 중성지방 억제 효과)

  • Kang, Ju-Won;Lee, Seung-Je;Ahn, Byung-Yong
    • Korean Journal of Plant Resources
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    • v.34 no.2
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    • pp.186-196
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    • 2021
  • In this study, we investigated inhibitory effect of red sweet pepper (Capsicum annuum L.) on triglyceride biosynthesis in Rhodosporidium toruloides. There was no significant difference in the total lipid content of all the experimental groups including 0.02, 0.1 and 0.5% (w/v) of red sweet pepper 70% ethanol extract treatment (RSPE). However, the triglyceride content was significantly decreased in RSPE group campared to the control group. When the formation of lipid droplet in the oleaginous yeast was examined, a small amount of fluorescence was observed compared to the control as the concentration of RSPE increased. The number of cells and free fatty acid increased in a concentration-dependent manner. These results suggest that RSPE has an anti-obesity effect.

Quality Characteristics of Ogapiju Prepared by Different Raw Materials (원료 첨가를 달리한 오가피주의 품질 특성)

  • Choi, Hyang-Sook;Min, Kyung-Chan
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.525-531
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    • 2005
  • Quality characteristics of ogapiju prepared by adding different raw materials such as Acanthopanax(0, 0.1, 0.25, 0.5%), Ganoderma lucidum(0, 0.05%), pine needle (0, 0.05%), and red ginseng(0, 0.05%) were evaluated by chemical analyses and sensory evaluation. Total organic acid contents of samples containing ogapi and other medicinal herbs were higher than that of control group. Major free amino acid was histidine. Contents of total amino acid and free sugar were highest in sample C (Acanthopanax 0.1, G. lucidum 0.05, pine needle 0.50, red ginseng 0.05%). In each sample 31-49 volatile components were identified by solid-phase microextraction method, and 42 components were detected by sniff-test using GC-olfactometry. Ogapiju showed higher content of ${\alpha}-copaene$ than control group ${\alpha}-Pinene$, camphene, ${\beta}-pinene$, sabinene, ${\alpha}-terpinene,\;{\gamma}-terpinene$, p-cymene, terpinolene, ${\alpha}-thujone,\;{\beta}-thujone,\;{\alpha}-terpineol$, carvone, and ${\beta}-ionone$ were not identified in control group. Volatile composition of ogapiju was characterized by higher amount of terpenoids. Green and herbaceous note was stronger in ogapiju than control group. Sensory evaluation indicated that good taste and palatability were highest in sample C.

Mono-granular Compound Fertilizer Acting Slow Release for the Crops Under Vinyl Mulching Cultivation -I. Effect of formulation and granulation on slow release (비닐멀칭작물재배용(作物栽培用) 지효성(遲效性) 전용복비(專用複肥) 개발(開發) -I. 제립(製粒)이 지효성(遲效性)에 미치는 영향(影響))

  • Shin, Jae-Sun;Lim, Dong-Kyu;Seong, Ki-Seog;Kim, Bok-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.97-100
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    • 1987
  • Medium grade mongranular compound fertilizers for the crops under vinyl mulching cultivation were formulated using the principal materials of urea, DAP and MOP in combination with various fillers of zeolite, gypsum and treated human waste and granulated with waste liquid of glutamic acid fermentation as binder. The ratios of $N-P_2O_5-K_2O$ of trial products were 12-10-11.5 for red pepper, 8-8-6 for sesame and 4-9-14 for peanut. The rate of dissolution of trial products was comparatively low in water. The product for red pepper had N dissolution rate of 80 percent during 24 hrs. 70 for peanut and 55 for seasame. The optimum dissolution rate has reported to be 50 percent by the standard laboratory dissolution test for 24 hrs. in water. Therefore, the slowly released characteristics were obtained with the products that had 24 hrs. dissolution of 70 to 60 percent. One time basal application of trial products could readily be justified under muching cultivation.

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Comparison of 10 Different Pre-Enrichment Broths for the Regeneration of Cronobacter spp. (Enterobacter sakazakii ) Infected in Powdered Infant Formula

  • Jung-Whan Chon;Kun-Ho Seo;Hyungsuk Oh;Dongkwan Jeong;Kwang-Young Song
    • Journal of Dairy Science and Biotechnology
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    • v.41 no.3
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    • pp.103-112
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    • 2023
  • This study aimed to assess the effectiveness of 10 different pre-enrichment methods using Real-Time polymerase chain reaction (PCR) in support of the FDA method. When the initial Cronobacter spp. (Enterobacter sakazakii) inoculation was 7.2 CFU/g, the Ct values were observed in the following order: 21.37 (Enterobacteriaceae enrichment [EE] broth), 21.95 (brain heart infusion [BHI]), 22.72 (tryptic soy broth [TSB]), 23.02 (violet red bile lactose [VRBL]), 22.31 (TSB-0.1% sodium pyruvate [SP]), 23.43 (distilled water [DW]), 24.34 (phosphate buffered saline [PBS]), 24.95 (nutrient broth [NB]), 25.82 (TSB-0.6% yeast extract [YE]), and 28.27 (violet red bile glucose [VRBG]). For an inoculation of 1.82% CFU/g of Cronobacter spp. (E. sakazakii), the Ct values were recorded in this sequence: 20.34 (EE broth), 22.16 (TSB-0.6% YE), 22.37 (BHI), 22.71 (VRBL), 22.88 (TSB), 23.01 (DW), 23.19 (NB), 23.79 (TSB-0.1% SP), 24.66 (VRBG), and 24.70 (PBS). Finally, when the inoculum of Cronobacter spp. (E. sakazakii) was 0.182 CFU/g, the Ct values followed this order: 21.93 (VRBL), 23.07 (TSB-0.6% YE), 23.31 (DW), 23.47 (PBS), 23.70 (BHI), 24.14 (TSB-0.1% SP), 25.14 (TSB), 29.00 (VRBG), 31.55 (EE broth), and were undetected in the case of NB. Consequently, these results indicate that there were no significant differences among the 10 different pre-enrichment broths. Future studies should focus on exploring pre-enrichment broths that can improve the limit of detection at very low Cronobacter spp. (E. sakazakii) concentrations and enhance the selective recovery of Cronobacter spp. (E. sakazakii) under acid, antibiotic, cold, and heat damage conditions.

The Effects for insecticide and synthesis of 5,6,8-trichloro-2,4-di-trichloro methyl benzo-1,3-dioxane (5,6,8-Trichloro-2,4-di-trichloromethyl-benzo-1,3-dioxane의 合成과 殺충能에 관하여)

  • Lee, Dae-Soo
    • Journal of the Korean Chemical Society
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    • v.10 no.2
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    • pp.62-66
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    • 1966
  • An insecticide was obtained from condensation of chloral hydrate with 2,4,5-trichloro phenol. The structure of the insecticide was found to be 5,6,8-trichloro 2,4-di-trichloro methyl benzo 1,3-dioxane. The best conditions of the condensation were as follows: 1) The sulfuric acid concentration; $97{\%}$. 2) The mole ratio of sulfuric acid to 2,4,5-trichloro phenol; 14.2. 3)The mole ratio of chloral hydrate to 2,4,5-trichloro phenol; 2.4. 4) The reaction time & reaction temperature;15hrs & $50-55^{\circ}C$.The insecticidal effects of T. D. B against the Citrus Red Mite and Green Peach Aphid were the same of Mydran.

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Effects of Ginseng Extract on Biological Cell Membrane and Artificial Lipid Monolayer (인삼추출물이 생체 세포막 및 artificial lipid monolayer에 미치는 영향)

  • Paik, Kwang-Sei;Lee, Chul-Young;Lee, Kyung-Nam;Song, Sun-Ok;Kang, Doo-Hee
    • The Korean Journal of Physiology
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    • v.10 no.1
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    • pp.7-14
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    • 1976
  • The Present study was conducted to investigate the effects of Ginseng extract on the tension-area curve for stearic acid monolayer. At the same time, the effects of Ginseng extract on osmotic and mechanical fragility of human red cells and histamine release from rabbit leukocytes were studied, The results are summarized as follows. 1. The Ginseng alcohol extract was found to expand liquid expanded phase of stearic acid monolayer, thus it is speculated that this agent may be acting as a surface active substance. 2. Osmotic hemolysis was inhibited by the Ginseng alcohol extract and the same effect was also observed in the presence of Ginseng saponin. However, the Ginseng alcohol extract was found to decrease hematocrit ratio of the RBC suspension, therefore, the inhibition of the osmotic hemolysis by this agent may be secondary effect to the reduced cell volume. 3. The mechanical hemolysis was also inhibited by the Ginseng alcohol extract but the inhibition was independent of changes in hematocrit ratio. 4. Histamine release from rabbit leukocytes was significantly increased in vitro in the presence of the Ginseng alcohol extract.(p<0.05)

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The Korean Traditional Medicine Gyeongshingangjeehwan Reduces Lipid Accumulation in Skeletal Muscle and C2C12 Cells

  • Yoon, Mi-Chung
    • Biomedical Science Letters
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    • v.17 no.4
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    • pp.283-289
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    • 2011
  • Our previous study demonstrated that the Korean traditional medicine Gyeongshingangjeehwan (GGEx) activates AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) critical for fatty acid oxidation in skeletal muscle and C2C12 skeletal muscle cells. Thus, we examined whether GGEx can reduce lipid accumulation in these cells and tissues. After obese and type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats were treated with GGEx, we studied the effects of GGEx on skeletal muscle lipid accumulation. The effects of GGEx and/or the AMPK inhibitor compound C on lipid accumulation and expression of AMPK and $PPAR{\alpha}$ were measured in C2C12 skeletal muscle cells. Compared with lean Long-Evans Tokushima Otsuka rats, obese OLETF rats had increased triglyceride droplets. However, administration of GGEx to OLETF rats for 8 weeks significantly decreased triglyceride droplets in skeletal muscle. Consistent with the $in$ $vivo$ data, GGEx inhibited lipid accumulation, the degree of which was comparable to Wy14,643, the potent activator of $PPAR{\alpha}$. GGEx also increased skeletal muscle mRNA levels of AMPK${\alpha}1$, AMPK${\alpha}2$, and $PPAR{\alpha}$. However, compound C inhibited these effects in C2C12 cells. These results suggest that GGEx suppresses skeletal muscle lipid accumulation and this process may be mediated by AMPK and $PPAR{\alpha}$ activation.

Ripening Related Changes in Hot Pepper Fruit Cell Walls Structural Alterations of Cell Wall Polysaccharides and Separation of Galactosidase Isozymes (고추의 성숙에 따른 세포벽 다당류의 변화와 ${\beta}-Galactosidase$ Isozymes의 분리)

  • Kim, Soon-Dong;Kang, Meung-Su;Kim, Kwang-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.2
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    • pp.157-163
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    • 1985
  • Various cell wall polysaccharides and related enzyme activities in hot pepper fruit were determined at different stages of maturity. The uronic acid content of cell walls decreased between immature green and turning stage fruit and then increased by red ripe stage. In contrast, cellulose content of cell walls changed only a little during ripening. Total neutal sugar content of cell wall material decreased 50% and galactose content of the walls decreased about 80% by the turning stage. Polygalacturonase and ${\beta}-galactosidase$ activities, as well as total hemicellulose from isolated cell walls of ripening hot pepper fruit were studied using gel filtration chromatography. Polygalacturonase activity was not detectable but 5 isozymes of ${\beta}-galactosidase$ were resolved. The activities of the enzymes were relatively high and gel filtration showed that they differed in molecular weight. Hemicellulose content decreased during ripening and softening. The molecular weight profiles shifted from high molecular weight to low molecular weight polymers during ripening. The changes in cell walls that may be associated with fruit softening involve the alteration of hemicellulose prior to the degradation of wall-bound uronic acid. It is suggested that the decrease in cell wall galactose involved changes in turnover of new cell wall components.

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Distribution of potential risky species on phytoplankton at ports in Korea (항만 환경에서 식물플랑크톤 잠재적 위해종의 분포)

  • Kwon, Oh Youn;Kang, Jung-Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.506-510
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    • 2013
  • This study aimed to understand relationship between potential risky species and environmental factors at ports in Korea. During the study periods, 25 potential risky species (red tide and toxic species) representing 20 red-tide species, 5 toxic species were observed in the all ports. Skeletonema costatum (red-tide species) was predominated in all study area. This species showed positive correlation with pH, while negative correlation with dissolved oxygen (p<0.05) at Busan port. Also, this species showed positive correlation with total suspended solids and pH (p<0.05) at Ulsan port. However, Sk. costatum showed positive correlation with nitrate at Incheon port (p<0.01). Pseudo-nitzschia spp. producing amnesic shellfish poison (domoic acid) showed positive correlation with nitrate and silicate in all study areas (p<0.05). Alexandrium spp. (paralytic shellfish poison) and Dinophysis acuminata (diarrhetic shellfish poison) were affected by chemical oxygen demand (p<0.01). Our results indicated that red-tide species were affected by physical factors, while chemical factors affected toxic species.

Quality Characteristics, Changes in Physiochemical Properties and Functional Properties of Camembert Cheese Containing Red Ginseng Powder

  • Lee, Jai-Sung;Bae, Inhyu
    • Food Science of Animal Resources
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    • v.38 no.1
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    • pp.64-77
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    • 2018
  • Effects of quality, physicochemical properties and antioxidants in Camembert cheese added with red ginseng powder (RGP) were investigated. Cheese samples were prepared with 0.05%, 0.10%, 0.15% and 0.20% RGP. and then monitored during ripening at $14^{\circ}C$ for 28 d. The pH of the RGP amended treatment groups increased during the ripening period relative to the control (p<0.05). Moreover, the 1,2-Diphenyl-1-picrylhydrazyle (DPPH) was highest in the 0.15% RGP group from 21 d to 28 d. $ABTS^+$ radical scavenging activity was increased just like DPPH as the ripening period passed, 0.10% treatment was highest at from 7 d to 21 d. 0.15% RGP was contents of ginsenosides : 10,999.7 ppm. The Free fatty acids (FFA), controls with 0.15% treatment, while the total fat (TF) and monounsaturated fat (MuSF) were higher in the control than the 0.15% RGP group (p<0.05). The total free amino acid (FAA) was increased in the control, and 0.15% RGP, and control was highest at then 0.15% RGP. The samples had average contents of fat and protein were 29% and 18-20% respectively. Additionally, the $L^*$ value decreased, while the $a^*$ and $b^*$ values increased as the amount of RGP added increased. Sensory evaluation revealed that texture and total acceptability were higher in the control group at 12 d. Although the addition of RGP did not exert a better effect on the ripening of the camembert cheese, but the ripening grade was similar to that of the common camembert cheese, and the additional function of the cheese was reinforced. Functional cheese could be developed.