• 제목/요약/키워드: Acer tegmentosum M.

검색결과 26건 처리시간 0.017초

Identification of Three Competitive Inhibitors for Membrane­Associated, $Mg^{2+}-Dependent$ and Neutral 60 kDa Sphingomyelinase Activity

  • Kim Seok Kyun;Jung Sang Mi;Ahn Kyong Hoon;Jeon Hyung Jun;Lee Dong Hun;Jung Kwang Mook;Jung Sung Yun;Kim Dae Kyong
    • Archives of Pharmacal Research
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    • 제28권8호
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    • pp.923-929
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    • 2005
  • Methanol extracts of domestic plants of Korea were evaluated as a potential inhibitor of neutral pH optimum and membrane-associated 60 kDa sphingomyelinase (N-SMase) activity. In this study, we partially purified N-SMase from bovine brain membranes using ammonium sulfate. It was purified approximately 163-fold by the sequential use of DE52, Butyl-Toyopearl, DEAE-Cellulose, and Phenyl-5PW column chromatographies. The purified N-SMase activity was assayed in the presence of the plant extracts of three hundreds species. Based on the in vitro assay, three plant extracts significantly inhibited the N-SMase activity in a time- and concentration-dependent manner. To further examine the inhibitory pattern, a Dixon plot was constructed for each of the plant extracts. The extracts of Abies nephrolepis, Acer tegmentosum, and Ginkgo biloba revealed a competitive inhibition with the inhibition constant (Ki) of $11.9 {\mu}g/mL,\;9.4{\mu}g/mL,\;and\;12.9{\mu}g/mL$, respectively. These extracts also inhibited in a dose-dependent manner the production of ceramide induced by serum deprivation in human neuroblastoma cell line SH-SY5Y.

Salidroside의 RAW 264.7 세포에서 $NF{-\kappa}B$ 불활성화를 통한 LPS에 (Inhibition of LPS induced iNOS, COX-2 and cytokines expression by salidroside through the $NF{-\kappa}B$ inactivation in RAW 264.7 cells)

  • 원소정;박희준;이경태
    • 생약학회지
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    • 제39권2호
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    • pp.110-117
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    • 2008
  • In this study, we investigated the anti-inflammatory effects of salidroside (SAL) isolated from the MeOH extract of Acer tegmentosum Maxim heartwood in RAW 264.7 macrophage cells. SAL pretreatment significantly inhibited nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) productions in the lipopolysaccharide (LPS)-induced RAW 264.7 cells. Western blot and RT-PCR analyses revealed that SAL inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, SAL reduced the release and the mRNA expressions of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6). Furthermore, nuclear factorkappa B ($NF{-\kappa}B$) luciferase reporter assay was performed to know the involvement of SAL in the production of pro-inflammatory cytokines, we confirmed that LPS-induced transcription activity of $NF{-\kappa}B$ was inhibited by SAL. Taken together, our data indicate that anti-inflammatory property of salidroside might be the result from the inhibition of iNOS, COX-2, $TNF-{\alpha}$ and IL-6 expressions via the down-regulation of $NF{-\kappa}B$ activity.

RAW 264.7 대식세포와 염증유도 동물모델에서 산겨릅나무 추출물의 항산화 및 항염증 효과 (In Vitro and In Vivo Anti-Oxidative and Anti-Inflammatory Activities of Acer tegmentosum Maxim Extracts)

  • 이초은;정현희;조진아;이선영
    • 한국식품영양과학회지
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    • 제46권1호
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    • pp.1-9
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    • 2017
  • 산겨릅나무 $80^{\circ}C$ 열수 및 95% 에탄올 추출물을 제조하여 항산화 및 항염증 효과를 확인한 결과 추출물은 우수한 항산화 효과를 나타내었고 RAW 264.7 세포에서도 항염증 효과를 보였으며, 그중 95% 에탄올 추출물의 효능이 더 큰 것으로 나타났다. 따라서 효능이 더 뛰어난 산겨릅나무 에탄올 추출물을 이용하여 in vivo 실험에서 추출물의 세포 보호효과 및 항염증 효능을 재확인하였다. ICR mouse에 일주일간 100 mg/kg B.W 산겨릅나무 에탄올 추출물을 경구투여한 후 LPS를 처리하여 염증반응 및 산화적 스트레스를 유도시켜 Comet assay, serum tumor necrosis $factor(TNF)-{\alpha}$$interferon-{\gamma}$, 장점막세포에서의 $TNF-{\alpha}$와 interleukin-6에 대한 생성능을 측정한 결과 산겨릅나무 에탄올 추출물은 DNA 손상을 억제하였으며 사이토카인 생성을 유의적으로 억제하여 항산화와 항염증 효과를 보였다. 따라서 본 연구에서 사용한 산겨릅나무의 에탄올 추출물은 산화적 스트레스 및 염증을 억제하는 효과를 나타내는 세포보호 기능적 소재로서 가능성이 있는 것으로 생각한다.

지리산국립공원 칠선계곡지역의 식생구조 (Vegetation Structure of the Chilseon Valley in the Jirisan National Park)

  • 추갑철;안현철;조현서;김임규;박은희;박삼봉
    • 한국환경생태학회지
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    • 제23권1호
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    • pp.22-29
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    • 2009
  • 지리산국립공원 칠선계곡지역의 식생구조를 파악하고자 천왕봉-선녀탕 구간 계곡부에 방형구($400m^2$) 23개를 설정하여 식생을 조사하였다. 식물군집을 분류한 결과 23개 조사구는 구상나무군집, 들메나무-당단풍군집으로 분류되었다. 천왕봉-마폭포구간에는 대부분이 구상나무가 우점하고 있었으며, 일부 지역에서 잣나무, 당단풍, 마가목 등의 침엽수와 낙엽수종들이 혼효하고 있었다. 수종간의 상관관계에서는 가문비나무와 마가목, 주목, 쇠물푸레, 음나무; 거제수나무와 물갬나무, 층층나무; 구상나무와 잣나무; 국수나무와 사스래나무, 산겨릅나무, 산벚나무; 당단풍과 사스래나무; 들메나무와 바위말발도리, 시닥나무; 마가목과 음나무; 물갬나무와 층층나무; 비목나무와 조록싸리, 졸참나무, 쪽동백나무; 생강나무와 전나무; 서어나무와 조록싸리; 쇠물푸레와 시닥나무, 잣나무, 주목; 시닥나무와 주목; 신갈나무와 철쭉; 졸참나무와 쪽동백나무 등의 수종들 간에는 높은 정의 상관관계가 인정되었고, 구상나무와 바위말발도리; 생강나무와 잣나무의 사이에는 부의 상관관계가 인정되었다. 조사지의 군락별 종다양성 지수(H')는 $2.7478{\sim}2.9573$ 범위로 비교적 높게 나타났다.

The Pattern of Natural Regeneration by Canopy Gap Size in the Mixed Broadleaved-Korean Pine Forest of Xiaoxing'an Mountains, China

  • Jin, Guangze;Tian, Yueying;Zhao, Fengxia;Kim, Ji Hong
    • 한국산림과학회지
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    • 제96권2호
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    • pp.227-234
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    • 2007
  • The forest canopy gap has been well known as a substantial process of forest cyclic regeneration and important role in stand structure, dynamics, and biodiversity of the forest ecosystem. Based on 3,600 $5m{\times}5m$ square grids in a 9ha permanent experimental plot, the study was conducted to evaluate the regeneration pattern of woody species by developmental stage {seedlings (<1 m of height), saplingI (>1 m of height, <2 cm of DBH), and saplingII (2 cm$<200m^2$), $201-400m^2$, $400-600m^2$, $601-800m^2$, and $>800m^2$) in the mixed broadleaved-Korean pine forest. The results indicated that the regenerating trees of Populus ussuriensis occurred only in the canopy gap area, considered to be a typical gap-dependent species. The regeneration of Ulmus japonica, Ulmus laciniata, and Maackia amurensis could be generally satisfied with the gap size of $201-600m^2$, Betula costata and Prunus padus with gap size of $401-800m^2$, Picea koraiensis with gap size of $201-800m^2$, Fraxinus mandshurica and Syringa reticulata var. mandshurica with smaller than $800m^2$, respectively. Acer ukurunduense and Acer tegmentosum were likely to have no problem with the gap size to make gap regeneration. Acer mono and Tilia amurensis looked more capable of regenerating in the closed canopy disregarding the upper crown condition. The regeneration of Pinus koraiensis and Abies nephrolepis had no trouble under the canopy condition in less than $800m^2$of gap size. The density of regenerating shrubs was rather high, especially under the closed canopy, considered to be associated with great amount of regeneration production in such shade tolerant species as Lonicera maackii, Corylus mandshurica, Euonymus pauciflorus, and Philadelphus schrenkii under the closed canopy. Pearson correlation coefficient was computed to compare the similarity among non-gap area and five gap size classes by developmental stages for trees and shrubs. The similarity coefficients among closed canopy and the gap size classes were mostly significantly correlated to each other with a few exceptions.

The Pattern of Seed Rain in the Broadleaved-Korean Pine Mixed Forest of Xiaoxing'an Mountains, China

  • Jin, Guangze;Xie, Xingci;Tian, Yueying;Kim, Ji Hong
    • 한국산림과학회지
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    • 제95권5호
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    • pp.621-627
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    • 2006
  • This study was conducted to understand the pattern and characteristics of seed rain in the broadleaved-Korean pine mixed forest. We established 287 $0.5m^2$ circular seed traps and collected and identified fallen seeds in the traps every two weeks in $150m{\times}150m$ sample plot of the permanent nine hectare of experimental plot in 2005. The overall average density of seed rain was $864.2/m^2$. The seeds of Betula costata Betula costata had the highest number of fallen seeds as $676.0/m^2$ (78.2%), followed by Abies nephrolepis as $57.5/m^2$, B. platyphylla as $37.9/m^2$, Tilia amurensis as $32.2/m^2$, Acer ukurunduense as $17.0/m^2$, A. tegmentosum $14.8/m^2$, and so on. Pinus koraiensis was recorded only $2.5/m^2$ of fallen seeds mainly owing of Korean pine had low rate of purity due to the animal and microbiological predation. Most of seed dispersal have started from the middle to late August and come to an end on the middle of November. The peak time of seed dispersal varied depending on the species. The rate of pure seed by dispersal time varied according to the species, thereupon the aspect of predation and the rate of blasted seed which had influence on the rate of purity also varied according to the species. The density of Korean pine seed rain in the forest gap was significantly different at $P{\leq}0.05$ from in the closed canopy. But the other species had no difference among canopy coverage.