• KIEAE Journal
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• v.16 no.3
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• pp.137-143
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• 2016

Purpose: Apartment housing should conduct a cyclic repair to keep and maintain the building performance since they are constructed. Therefore, the repair plan would be provided for long term period which explains the repair time, items and repair cost. Residents of apartment housing are responsible to pay for the repair activities. For repair cost, residents would reserve the money for repair little by little continuously until the required repair time because the repair cost takes a big burden for residents and lots of money a time. But, there is no systematic approach to provide the long term repair cost because it is no proper forecast of the repair cost to the upcoming repair time. In this study, it aimed at providing the monthly accumulation of the long term repair cost with the survey data in Seoul. Method: For these, the surveyed data are classified into 6 categories and number of data are 1,918. In addition, it developed the repair cost model for the 24 repair works and the cumulation function which is reflected with the each cost model. Result: This study are shown as follows : First, among the various estimation for the repair cost, the power function has a goodness of fit in statistics. Second, the monthly accumulation would be 12,840 won/household in size of $100,000m^2$ management area and $81.7won/m^2$ in size of the 1,000 household number during 40 years.

• BMB Reports
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• v.40 no.3
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• pp.373-381
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• 2007

Seed storage proteins (SSPs) are important for seed germination and early seedling growth. We studied the accumulation and degradation profiles of four major Arabidopsis 12S SSPs using a 2-DE scheme combined with mass spectrometric methods. On the 2-DE map of 23 dpa (days post anthesis) siliques, 48 protein spots were identified as putative full-length or partial $\alpha$, $\delta$ subunits. Only 9 of them were found in 12 dpa siliques with none in younger than 8 dpa siliques, indicating that the accumulation of 12S SSPs started after the completion of cell elongation processes both in siliques and in developing seeds. The length and strength of transcription activity for each gene determined the final contents of respective SSP. At the beginning of imbibition, 68 SSP spots were identified while only 2 spots were found at the end of the 4 d germination period, with $\alpha$, subunits degraded more rapidly than the $\alpha$ subunits. The CRC $\delta$ subunit was found to degrade from its C-terminus with conserved sequence motifs. Our data provide an important basis for understanding the nutritional value of developing plant seeds and may serve as a useful platform for other species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.2
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• pp.61-65
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• 1971

It is well known that shellfish absorb and accumulate heavy metals in the water environment. When the concentration of the heavy metals in the water is above guideline, we find abnormal accumulation of them in the body of shellfish. This study on green oysters is an instance showing the relationship between the abnormal accumulation of heavy metal and industrial wastewaters containing it. The influence of the industrial wastewaters upon shellfish has been discussed from the statistical, physiological, and saprobiensystematic points of view, and the author wants to make clear of the absorption and accumulation mechanism of the heavy metals and physiological situation. This paper deals with the copper-contents in the meat of oysters which were collected in Changhang Bay and Daechun area, the latter being not supposed to be influenced by the water of the Kum River during the period from October 1970 to february 1971. The mean value of the copper contents in oysters caught at the sampling station in the Changhang Bay area is ranged from 851.5 to 143.1mg Cu/kg wet base, and that of Daechun area varied from 13.0 to 27.4mg Cu/kg wet base, so an evident difference is seen at a significance of $0.1\%$. It appears that one of the environmental factors making copper concentrated in the oysters of the Changhang area is due to the copper-contaning slug being discharged into the sea water by the Changhang Ore Refinery.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.409-419
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• 2016

With increasing social concerns for healthy food, the studies on the cultivation of crops to increase accumulation of functional metabolites in crops have been investigated. Accumulation of the metabolites in crops is highly affected by various types of stress, such as nutrient deficiency, water deficit (WD), extreme temperature and UV-B radiation as well as their own life cycle. This review summarizes the previous studies on the effects of environmental stresses, especially WD and UV-B radiation, on accumulation of functional metabolites in crops. UV-B radiation and WD during specific period (mainly at maturation stage) activates the adaptation and/or defense system in crops, thereby increasing biosynthesis of the metabolites. Although WD and UV-B radiation tend to decrease in crop yield, the decrease can be compensated by the production of high value crops having high content of functional metabolites.

• Archives of Pharmacal Research
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• v.28 no.7
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• pp.823-828
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• 2005

Multidrug resistance (MDR) is one of the most significant obstacles in cancer chemotherapy. One of the mechanisms involved in the development of MDR is the over-expression of P-glycoprotein (P-gp). It is widely known that natural compounds found in vegetables, fruits, plant-derived beverages and herbal dietary supplements not only have anticancer properties, but may also modulate P-gp activity. Therefore, the purpose of this investigation was to examine the effects of naturally occurring products on P-gp function in human breast cancer cell lines, MCF-7 (sensitive) and MCF-7/ADR (resistant). The accumulation of daunomycin (DNM), a P-gp substrate, was greater in the sensitive cells compared to the resistant cells, while the efflux of DNM was higher in the resistant cells compared to the sensitive cells over a period of 2h. The $IC_{50}$ value of DNM in the resistant cells was about 22 times higher than that in the sensitive cells, indicating an over-expression of P-gp in the resistant cells, MCF-7/ADR. All of the compounds tested, with the exception of fisetin, significantly decreased the $IC_{50}$ value of DNM. Biochanin A showed the greatest increase in $[^3H]-DNM$ accumulation, increasing by $454.3{\pm}19.5%$ in the resistant cells, whereas verapamil, the positive control, increased the accumulation by $229.4{\pm}17.6%$. Also, the accumulation of $[^3H]-DNM$ was increased substantially by quercetin and silymarin while it was reduced by fisetin. Moreover, biochanin A, silymarin, and naringenin significantly decreased DNM efflux from MCF-7/ADR cells compared with the control. These results suggest that some flavonoids such as biochanin A and silymarin may reverse MDR by inhibiting the P-gp function.

• Journal of fish pathology
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• v.17 no.1
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• pp.21-28
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• 2004

In late summer of 2001, a new disease "overturn disease" occurred among mud loach (Misgurnus mizolepis) cultured in a farm Chonbuk Province. The fish exhibited a lethargic, erratic and spiral swimming at the water surface. Affected fish showed swollen abdomen with accumulation of air bubbles in the intestinal canal. The bubbles were much larger in the anterior part than in the posterior part. Hematological and histopathological examinations were done. No significant differences were found in RBC count, Ht value and Hb concentration between the diseased and the healthy fish. Gill filaments showed proximal hyperplasia of the respiratory epithelia and fusing of the gill lamella. Hemorrhage and necrosis occurred in the intestinal epithelia and within the lamina propria, and the mucosal epithelia were separated from the muscularis. Hepatocytes underwent atrophy. In the fish experimentally tied between the annal fin and the anus by a thread to confirm accumulation of air bubbles within intestine, accumulation of air bubbles was confirmed. This disease in mud loach appeared to be induced by circulatory disturbances in the intestinal wall owing to accumulation of air bubbles in the alimentary canal after fed high protein diet throughout all culturing period.

• Journal of Periodontal and Implant Science
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• v.52 no.1
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• pp.65-76
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• 2022

Purpose: The present study measured changes in arteriolar and venular capillary flow and structure in the gingival tissues during the development of plaque-induced gingival inflammation by combining dynamic optical coherence tomography (OCT), laser perfusion, and capillaroscopic video imaging. Methods: Gingival inflammation was induced in 21 healthy volunteers over a 3-week period. Gingival blood flow and capillary morphology were measured by dynamic OCT, laser perfusion imaging, and capillaroscopy, including a baseline assessment of capillary glycocalyx thickness. Venular capillary flow was estimated by analysis of the perfusion images and mean blood velocity/acceleration in the capillaroscopic images. Readings were recorded at baseline and weekly over the 3 weeks of plaque accumulation and 2 weeks after brushing was resumed. Results: Perfusion imaging demonstrated a significant reduction of gingival blood flow after 1 and 2 weeks of plaque accumulation (P<0.05), but by 3 weeks of plaque accumulation there was a more mixed picture, with reduced flow in some participants and increased flow in others. Participants with reduced flux at 3 weeks also demonstrated venular-type flow as determined by perfusion images and evidence of the development of venular capillaries as assessed by the velocity/acceleration ratio in capillaroscopic images. After brushing resumed, these venular capillaries were broken down and replaced by arteriolar capillaries. Conclusions: After 3 weeks of plaque accumulation, there was wide variation in microvascular reactions between the participants. Reduced capillary flow was associated with the development of venular capillaries in some individuals. This is noteworthy, as an early increase in venous capillaries is a key vascular feature of cardiovascular disease, psoriasis, Sjögren syndrome, and rheumatoid arthritis-diseases with a significant association with the development of severe gingival inflammation, which leads to periodontitis. Future investigations of microvascular changes in gingival inflammation might benefit from accurate capillary flow velocity measurements to assess the development of venular capillaries.

• Journal of Ginseng Research
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• v.48 no.1
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• pp.103-111
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• 2024

Background: Ginseng (Panax ginseng Mayer) is an important natural medicine. However, a long culture period and challenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainable medicinal supply, research on the association between the transplantation and chaining of metabolic networks, especially the regulation of ginsenoside biosynthetic pathways, is limited. Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurements to evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of genetic alterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtained by MapMan, we constructed a metabolic profile of transplantation Ginseng. Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but following transplantation, the ginsenoside content increased significantly from 6-8 years (in the wild). Glycerolipid metabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids and lipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathways during transplantation Ginseng growth. Conclusion: This study expands our understanding of metabolic network features and the accumulation of specific compounds during different growth stages of this perennial herbaceous plant when growing in transplantation mode. The findings provide a basis for selecting the optimal transplanting time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.324-330
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• 1994

The objective of this study is to obtain the basic data for investigating the potentiality of continuous utili~ation (first cutting in the late fall and regrowth yield in next spring) of forage rape seeded in fall. Fresh yield and the content of nitrogenous compound in leaves and roots were measured during a growth period. Fresh yield accumulation was very low between the late fall(470kg/10a) and the early regrowth period( 1.070kg /IOa). Total nitrogen content in leaves until the wintering period was decreased from 4.71% to 3.70%. while that in roots slightly increased during this period. The highest content(4.84%) in roots was observed in the early regrowth period, and then rapidly decreased as growth advanced. Protein-N was the largest pool of nitrogenous compound in leaves and roots through entire growth period, Its content in leaves decreased until the wintering period (Feb. 4), and then increased until the bolting stage(Apr. 10). Protein-N in roots highly accumulated from the late fall(11.1mg/gDM) to the early regrowth period(l6.9mg/gDM), and then decreased until the early tlowering stage. The content of amino acid-N in leaves showed a little change with a range from 5.7 to 8.5mgIgDM during entire growth period, while that in roots rapidly decreased from early regrowth period. The content of $NO_3$-N decreased from 7.0 to 4.3mglgDM in leaves, while increased from 0.9 to 2.3mg/gDM in roots from the late fall to the wintering period. 7he content of $NH_4$-N was lower and less varillble than other nitrogen compound during entire growth period. The results clearly showed that protein-N was the main storage form and highly accumulated in roots of overwintering forage rape.

• Preventive Nutrition and Food Science
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• v.14 no.2
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• pp.142-147
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• 2009

In this study, the selenium (Se) accumulations of soybean sprouts and mungbean sprouts treated with various concentrations of Se-solutions were evaluated, as part of a broader effort to produce Se-enriched variants of the plants. Four levels of sodium selenate ($Na_{2}SeO_{4}$)-dissolved solutions (i.e. 0, T0; 6, T1; 60, T2; and $600{\mu}g/mL$, T3) were prepared and sprayed onto the plants during cultivation. The effect of different spraying periods on Se accumulation was also assessed by watering plant groups once a day for periods of one, two, or three days. Se solution remaining on the surfaces of the plants was washed out by spraying with distilled water on the final day of cultivation. However, the increase of Se accumulation in the plants was found to depend on both Se-concentration and watering period, and the soybean sprouts were determined to accumulate Se more effectively than the mungbean sprouts. Additionally, with regard to Se accumulation in the plants, the period of application of Se solution was determined to be more important than the concentration of the Se solution applied. The averaged total levels of Se-enrichment in whole soybean sprouts at T0, T1, T2, and T3 were 0.26, 65.86, 179.62, and $525.12{\mu}g/dry$ matter (DM) g, respectively, and the relative equations relating Se enrichment in soybean sprouts (Y) against watering days (X) were Y=32.505X-36.17 (T1), Y=88.46X-92.04 (T2), and Y=251.11X-254.9(T3). The averaged total levels of Se-enrichment in the whole mungbean sprouts at T1, T2, and T3 group were 0.05, 3.64, and $101.43{\mu}g/DM$ g, respectively, and the relative equations relating Se enrichment (Y) to watering days (X) for mungbean sprouts were Y=1.67X-1.3467 at T1 and Y=48.035X-46.907 at T2. The results of this study suggest that soybean sprouts and mungbean sprouts enriched with bioavailable Se can be produced on a large scale by Se supplementation, allowing for the development of healthy functional foods such as Se-enriched mungbean sprout soups and salads, Se-enriched functional drink and food additives, and selenium tablets to promote health.