• Applied Biological Chemistry
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• v.48 no.2
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• pp.166-172
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• 2005

In order to prepare liqueur of Acanthopanax koreanum, changes in major constituents by soaking below 0.5 cm size dried sample 700 g in 10 l of $15{\sim}95%$ spirit solution for 70 days were investigated. Color b was increased according to lower ethanol concentration and longer soaking periods. Extract was increased gradually with soaking periods, and the content was $0.6{\sim}0.7%$ (w/v) with stem, $1.0{\sim}1.5%$ (w/v) with root. Eleutheroside B and E were extracted rapidly within 20 days of soaking, moreover were increased according to ethanol concentration within 15% to 70%. Acantoic acid was extracted rapidly $2.8{\sim}22.6\;{\mu}g/ml$ with stem, and $560{\sim}1,700\;{\mu}g/ml$ with root within 5 to 10 days. For preparation of liqueur of Acanthopanax koreanum, it is necessary to soak more portion of dried root with $60{\sim}80%$ ethanol concentration for $30{\sim}50$ days, and then to blend after aging for 13 weeks.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.95-95
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• 2003

• Applied Biological Chemistry
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• v.44 no.1
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• pp.24-29
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• 2001

Extracts of Acanthopanax koreanum Nakai were decreased as the ethanol concentration was increased, but eleutherosides were increased. Extracts were increased as the extraction time was prolonged. Compared to 15 hrs extraction, extract yield and eleutherosides for 5 hrs extraction were 90.6% and 96%, respectively. Yields of extract and eleutherosides were 6.5% and 75% at $100^{\circ}C$. The optimum extraction conditions were obtained from the samples of 4 years' tree harvested at September, which were water and 50% ethanol as solvents, and $90^{\circ}C$ for 5 hrs extraction. Soluble solids, minerals, free sugars, free acids, free amino acids and total eleutherosides in the extract of Acanthopanax koreanum were $48.2{\sim}47.4%$, $4.5{\sim}5.3%$, 11.2711.80%, $3.02{\sim}3.18%$, 185.33 mg/100 $g{\sim}$ 348.14 mg/100 g and 990.84 mg/100 $g{\sim}$l,416.10 mg/100 g, respectively. The extract was viscous and yellowish brown liquid.