• 식물조직배양학회지
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• 제27권1호
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• pp.39-45
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• 2000

전위유전자 표지를 이용한 2배체 야생근연종 감자의 유용 유전자 cloning 체계를 개발하기 위하여 옥수수의 진위유전자 Ac를 조작하여 전위효소는 생산하나 이동은 불가능하도록 제작된 immobilized Ac (iAc)와 자체 이동은 불가능하나 iAc의 작용에 의해 이동이 가능한 Ds를 Agrobacterium tumefaciens를 이용한 형질전환에 의해 2배체 근연종 감자 (Solanum tuberosum Group Phureja) 계통 1.22에 도입하였다. iAc및 Ds 삽입 binary vector들을 보유하는Agrobacterium 계통들로 형질전환 처리된 1.22 기내배양신초의 잎과 줄기 절편의 경우 50mg/L 의 kanamycin이 첨가된 배지에서도 캘러스를 형성하였으며, 잎 절편으로부터 재분화신초가 획득되었다. 형질전환 처리되지 않은 1.22 신초는 100mg/L의 kanamycin이 첨가된 배지에서 전혀 발근이 되지 않았으나, 형질전환 처리에 의해 획득된 재분화 신초는 동일 배지에서 발근이 되었다. iAc와 Ds의 염기배열에 대해 특이적인 oligonucleotide primer들을 이용하여 형질전환 처리 획득 식물들로부터 추출된 DNA에 대한 PCR분석을 실시한 결과, 사용된 primer들의 iAc 와 Ds의 염기배열에 있어서의 위치에 의해 예상되는 크기의 DNA들이 형성되어 iAc 와 Ds의 1.22 genome내 도입이 확인되었다.

• Journal of Power Electronics
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• 제8권1호
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• pp.1-9
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• 2008

This paper presents the design and analysis of a new input AC current wave shaping AC-DC converter for cost effective harmonic mitigation under varying loads. The proposed converter consists of a delta-polygon connected autotransformer based twelve-pulse AC-DC converter and a small rating passive shunt filter tuned at $11^{th}$ harmonic frequency. The proposed AC-DC converter eliminates the most dominant $5^{th},\;7^{th}$ and $11^{th}$ harmonics and reduces higher order harmonics; thereby, resulting in an improved power quality at AC mains. Moreover, the design of the autotransformer is modified to make it suitable for retrofit applications, where presently a 6-pulse diode bridge rectifier is used. To validate the proposed approach, various power quality indices are presented under varying loads. Experimental results obtained on the developed converter are given to validate the model and design of the proposed converter.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.11-15
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• 1999

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1,700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.51-57
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• 1985

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1, 700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• 생명과학회지
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• 제23권5호
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• pp.609-615
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• 2013

글리세올린 I은 다양한 피부 질환의 예방과 치료에 유용한 물질로 주목을 받아왔다. 그러나 피부 멜라닌 형성에 결정적 역할을 담당하는 포유류의 아데니닐 고리화 효소(이하 mAC)에 대한 직접적인 결합 형태와 상호 작용에 대한 연구는 현재까지 연구 보고된 사례가 없었다. 글리세올린 I 의 mAC 활성 부위에 대한 결합 작용 기작을 규명하기 위하여 우선 글리세올린 I과 SQ22,536 (mAC에 결합하여 mAC의 활성을 억제하는 것으로 이미 잘 알려진 단일 화합 물질)의 mAC에 대한 결합 친화도와 결합 형태를 비교 분석 하였다. 글리세올린 I은 mAC의 활성부위에 존재하는 Asp 1018, Trp 1020, Asn 1025와 각각 3개의 수소결합을 형성하는 것으로 분석되었고 SQ22,536은 mAC 활성부위의 Asp 1018, Asn 1025와 2개의 수소결합을 형성하여 글리세올린 I이 상대적으로 우월하게 결합하는 것으로 분석되었다. 글리세올린 I은 또한 포스콜린(forskolin)에 의해서 유도되는 세포내 멜라닌 형성 2차 신호전달 물질인 cyclic AMP의 생성과 이로인해 유발되는 단백질 인산화 효소 A의 인산화를 효과적으로 억제하는 것을 멜라노마 세포 실험을 통하여 확인하였다. 또한 글리세올린 I 을 장시간 세포에 투여하여도 세포의 생존률에는 영향을 주지 않음을 확인하였다. 본 연구 통하여 규명된 글리세올린 I의 mAC 활성 억제 효능 및 멜라닌 생성 신호전달 기작을 조절하는 성질을 이용하여 향후 흑색종과 같은 다양한 피부 질환 치료제 개발 및 미백화장품 핵심 물질 개발에 활용될 수 있을 것으로 사료된다.

• 한국독어학회지:독어학
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• 제7집
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• pp.105-125
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• 2003

Personal- und Reflexivpronomen stehen in einer komplementaren Beziehung zueinander. Die vorliegende Arbeit zeigt, welches von beiden Pronomen verschiedenen AcI-Konstruktionen jeweils richtig ist Vor allem stelle ich hier die These auf, dass Grammatik in erster Linie ein Regelwerk ist und dass dernzufolge $S\"{a}tze$ mit gleicher Struktur immer einheitlich und konsistent mit denselben Regeln $erkl\"{a}rt$ werden sollten: (1) a. Der Gefangen $l\"{a}sst$ den Polizisten auf ${\ast}sich_i/ihn_i$ achten. b. $Hans_i{\;}l\"{a}sst$ Maria zu $sich_i/{\ast}ihm_i$ kommen. (2) a. $Peter_i$ sieht Hans von ${\ast}sich_i/ihm_i$ betrogen. b. $Hans_i{\;}f\"{u}hlt$ die Freundin von $sich_i/{\ast}ihm_i$ weggezogen. Bei diesen Beispielen liegt der Verteilungsunterschied der Pronomina nicht an Grammatikregeln sondern am individuellen Sprachgebrauch. Ferner wird hier darauf hingewiesen, dass die AcI-Verben mit Ausnahme des Verbs lassen a1s 3-wertige Verben so wie die $S\"{a}tze$ in Beispiel (3) zu behandein sind D.h., die Struktur $f\"{u}r$ AcI-Konstruktionen sieht so aus wie (4b), nicht wie (4a). Betrachten wir dies noch einmal an einem Beispiel: (3) a. Ich sehe mich $m\"{u}de$ b. Ich $f\"{u}hle$ mich viel besser.

• 한국초전도ㆍ저온공학회논문지
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• 제7권3호
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• pp.29-33
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• 2005

Critical current$(I_c)$ degradation of High $T_c$ Superconducting(HTS) tapes and AC loss under mechanical load is one of the hottest issues in HTS development and application. Mechanical load reduces the critical current of superconducting wire, and the $I_c$ degradation affects the AC loss of the wire. We measured the $I_c$ degradation and AC loss under tension and bending of Bi-2223 tapes made by 'Powder-in-Tube' technique at 77K with self-field. Also, we have studied the frequency characteristics on self-field AC loss in multi-filamentary Bi-2223/Ag tape at 77K. The measurement results and discussions on the relationship between $I_c$ degradation and AC loss are presented.

• Journal of Electrical Engineering and Technology
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• 제4권1호
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• pp.111-117
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• 2009

This paper presents the characteristics of leakage currents flowing through zinc oxide (ZnO) surge arrester elements under the combined direct-current (DC) and 60 Hz alternating-current (AC) voltages. The current-voltage characteristic curves (I-V curves) of the commercial ZnO surge arrester elements were obtained as a function of the voltage ratio a. At constant peak value of the combined DC and AC voltage, the resistive leakage current of the ZnO blocks was significantly increased as the voltage ratio $\alpha$ increased. The I-V curves under the combined DC and AC voltages were placed between the pure DC and AC characteristics, and the cross-over phenomenon in both the I-V curves and R-V curves was observed at the low current region. The ZnO power dissipation for DC voltages was less than that for AC voltage in the pre-breakdown region and reversed at higher voltages.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.76-76
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• 2003

We report here the results on N-acetyl-N'-methylamide of 4-fluoroproline (Ac-Flp-NHMe) calculated using the ab initio molecular orbital method with the self-consistent reaction field (SCRF) theory at the HF level with the 6-31+G(d) basis set to investigate the stereoelectronic effects on the conformational preference of proline depending on the cis/trans peptide bonds and down/up puckerings along the backbone torsion angle $\square$ in the gas phase, chloroform, and water. In the gas phase, all potential energy surfaces for Ac-Flp-NHMe are quite similar to those of Ac-Pro-NHMe, except that up-puckered conformations are more stabilized than down-puckered ones. In chloroform and water, polyproline structures become dominant, whose populations are larger than those of Ac-Pro-NHMe. In chloroform and water, the populations of polyproline II (i.e., tF conformations) are quite similar to each other, but those of polyproline I (i.e., cF conformations) are larger by 5％ in water than in chloroform. In particular, all cis populations for Ac-Flp-NHMe in the gas phase, chloroform, and water are decreased than those of Ac-Pro-NHMe.

• 한국초전도ㆍ저온공학회논문지
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• 제9권4호
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• pp.41-45
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• 2007

Superconductor is developed for applications in high-power devices such as power-transmission cables, transformers, motor and generators. In such applications, HTS tapes are subjected to various kinds of stress or strain. AC loss is also important consideration for many large-scale superconducting devices. In the fabrication of the devices, the critical current $(I_c)$ of the high temperature superconductor degrades due to many reasons including the tension applied by bending and thermal contraction. These bending or tension reduces the $I_c$ of superconducting wire and the $I_c$ degradation affects the AC loss of the wire. The $I_c$ degradation and AC loss (self field loss) of Bi-2223 HTS and Coated conductor were measured under tension and bending conditions at 77K and self-field.