• Applied Biological Chemistry
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• v.22 no.4
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• pp.191-197
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• 1979

${\beta}-Tyrosinase$ was purified and crystallized from cells of Escherichia intermedia A-21 grown in a medium supplemented with 0.2% L-tyrosine. Molecular weight of its subunit, Km value and absorption spectra were determined. Crystallization methods were also studied to eliminate any unnecessary procedures. The results obtained were as follows: 1. The purification procedure included ammonium sulfate fractionation, dialysis against potassium phosphate buffer, pH 6.0 and pH 7.0, and DEAE-Sephadex column chromatography. In the column chromatography, 11 mg of protein was applied per ml of DEAE-Sephadex for efficiency. 2. Steps of protamine sulfate treatment and Sephadex G-150 gel filtration could be eliminated for this enzyme from the known procedures. 3. The purified enzyme was dissolved in 0.01M potassium phosphate buffer containing 2-mercaptoethanol, with a concentration of 20mg/ml. Crystalline enzyme, which appears as hexagonal rods, was obtained by adding solid fine powdered ammonium sulfate to the enzyme solution. 4. Absorption maxima of the enzyme appeared at 340 and 430nm when associated with pyridoxal phosphate. 5. Km value of the enzyme for L-tyrosine was $2.31{\times}10^{-4}M$ and the molecular weight of its subunit was determined by SDS-polyacrylamide electrophoresis to be approximately 50,000.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.2 no.2
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• pp.145-153
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• 2004

H$_2$SO$_4$ and citric acid were used as additives for the electrokinetic remediation experiment to increase removal efficiency of $^{137}$ Cs and $^{60}$ Co from the radioactive soil waste stored for more than 10 years. The average effluent velocity discharged from the elctrokinectic remediation experimental column was 2.0${\times}$10$^{-2}$ cm/min and the discharged soil wastewater volume for 10 days is 3.6 pore volume of the column. 97% of $^{60}$ Co in the column was decontaminated for 10 days of operation, while only 54% of $^{137}$ Cs was decontaminated. These results are considered that the absorption equilibrium coefficient of $^{137}$ Cs is higher than that of $^{60}$ Co. The predicted values of the residual concentration by the proposed mathematical model were well coincided with the experimental results within the experimental error range

• Earthquakes and Structures
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• v.6 no.1
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• pp.89-110
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• 2014

Nonlinear dynamic analyses are carried out to investigate the influence of the pinching hysteretic response of the exterior RC beam-column joints on the seismic behavior of multistory RC frame structures. The effect of the pinching on the local and global mechanisms of an 8-storey bare frame and an 8-storey pilotis type frame structure is evaluated. Further, an experimental data bank extracted from literature is used to acquire experimental experience of the range of the real levels that have to be considered for the pinching effect on the hysteretic response of the joints. Thus, three different cases for the hysteretic response of the joints are considered: (a) joints with strength and stiffness degradation characteristics but without pinching effect, (b) joints with strength degradation, stiffness degradation and low pinching effect and (c) joints with strength degradation, stiffness degradation and high pinching effect. For the simulation of the beam-column joints a special-purpose rotational spring element that incorporates the examined hysteretic options developed by the authors and implemented in a well-known nonlinear dynamic analysis program is employed for the analysis of the structural systems. The results of this study indicate that the effect of pinching on the local and global responses of the examined cases is not really significant at early stages of the seismic loading and especially in the cases when strength degradation in the core of exterior joint has occurred. Nevertheless in the cases when strength degradation does not occur in the joints the pinching may increase the demands for ductility and become critical for the columns at the base floor of the frame structures. Finally, as it was expected the ability for energy absorption was reduced due to pinching effect.

• Journal of the Korean housing association
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• v.3 no.2
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• pp.89-99
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• 1992

We discussed the change of housing environmental problems from the early 1900s to the present in Study(I). This study(II) which secendly research of study(I) analyzed the fixing precess of a lavatory centering around a excretion which was a serious housing environmental problem in 1920 to 1940. The documentary research method was used for this study. Articles of content analysis(N=185) were published in 1920 to 1990 which were The Deng-A daily news article about a excretion and a lavatory. The main content of this study was examined the change, such as the number of whole article, the column number of article by time series. and the content of article by subject. 1. The number of whole article by time series was collected mainly in1920s-1930s. In 1940s-1960s, one-two column of article was appeared generally and three-four, five column of article was appeared in 1970-1980. 2. Contents of article was divided into two classes, excretion and lavatory. Contents of excretion was 1) a use of fertilizer 2) the method of transportation 3) a cost of gathering 4) a place of disposal 5) the problem of cleaning. Contents of lavatory was 1) a public lavatory 2) a flush toilet 3) a sanitary conditions 3. 1) A use of fertilizer was concentrated in 1920s-1930s, and problems of it was solved more or less by change of management method. Transportation of excretion developed such as \ulcornerGue\ulcorner->a coach of tank style->underground transportation->a dung car of absorption style. Disposal place of excretion was a cause of dissatisfaction in 1920s and it is serious problem Today. A duty of excretion gathering was transfered to a private worker in 1978. The accunulation problem of excretion was continued until 1940s-1950s. The management law of waste matters was proclaimed in 1986. 2) A public lavatory was planned in 1924 for the first time, and it is insufficient in these days, A settlement of public lavatory in building which has upward of 20pyung became obligation in 1973. The problem of water contamination which by poor septic tank was happened in 1970s-1980s.

• Journal of Korean Society of Steel Construction
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• v.13 no.1
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• pp.71-79
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• 2001

The concrete filled tubular(CFT) columns have many excellent structural properites. such as high compressive strength high ductility and high absorption capacity However the confinement effect and limiting width-thickness ratio of CFT column have not yet been clarified. Therefore. this paper aims to clarify the confinement effect of steel tubes and strength of concrete filled steel tubular columns. And this paper presents results of a probabilistic analysis based on statistical data for strength of concrete filled steel tubular columns which has been tested in Korea for recent 10 years(1991.1~2000.6).

• Journal of Korean Society of Steel Construction
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• v.22 no.4
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• pp.355-363
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• 2010

The objective of this study is to examine the structural performance on the Rectangular Steel Tube Column-to-H Beam connections using one-side bolts and T-stub. Although a rectangular steel tube comparing with a H-shaped steel has many advantages and is more efficient, its application is limited due to the lack of experiences and connection details. Existing steel moment connections using the rectangular steel tube are mainly using through plate diaphragms. Its processing of construction is so complicated that it is hard to apply in the field. In this study, the structural performance and the earthquake capacity for T-stub connection with one-side bolts were investigated. And it is performed a comparative analysis of strength, rigidity, total rotation and energy absorption capacity for the various connection details.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.6 no.4
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• pp.29-42
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• 1986

This study is an experimental research on the adsorption capacity and the adsorption system utilizing Discarded Automotive Tires(DAT) and Powdered or Granular Activated Carbon(PAC or GAC) for the removal of heavy metals, Ag(I), Cd(II), Cu(II), Zn(II). Batch shaking test was conducted to determine the adsorption capacity of DA T and PAC in removing the heavy metals from aqueous wastes; and laboratory-scale column experiment was performed to present design factors affecting the optimum design of adsorption column with DAT and GAC, through the concept of Bed Deph/Service Time(BDST). As results, DAT has been proven to be a good adsorbent will its adsorption capacity not falling behind PAC or GAC. Factors affecting heavy metals removals were amount of adsorbents, initial concentrations, pH and so on. BDST equations were compared with values presented by the breakthrough data from adsorption system.

• Journal of Korean Society of Steel Construction
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• v.15 no.5 s.66
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• pp.531-539
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• 2003

Unlike field-welded moment frames damaged during the Northridge earthquake, a column-tree moment frame has a tool to control and reduce its seismic behavior. The tool is the girder splice. Girder splices could be designed to be sufficiently ductile and to have a prescribed bending moment capacity. In such a design, during earthquakes, the girder splices would act as ductile "fuses" and limit the magnitude of forces including the bending moment that could be developed in the frame. In Korea, most moment frames arc composed of a column-tree moment frame. Therefore, the elasto-plastic behavior of steel beams with high strength bolted friction splice should be clarified. Furthermore, structural capacities, including energy absorption capacity, must be quantitatively found. This paper discusses an experimental study to clarify elasto-plastic behavior of steel beams with high strength bolted friction splices. A total of 5 specimens were tested. A specimen was fabricated to have a beam splice designed by a full strength method. Other specimens were fabricated to have beam splices with 75%, 50% and 0% capacities compared with the specimen.

• Earthquakes and Structures
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• v.15 no.6
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• pp.617-627
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• 2018

After earthquakes FRP sheets are often used for the rehabilitation of damaged Reinforced Concrete (RC) beamcolumn connections. Connections with minor to moderate damage are often dealt with by applying FRP sheets after a superficial repair of the cracks using resin paste or high strength mortar but without infusion of thin resin solution under pressure into the cracking system. This technique is usually adopted in these cases due to the fast and easy-to-apply procedure. The experimental investigation reported herein aims at evaluating the effectiveness of repairing the damaged beam-column connections using FRP sheets after a meticulous but superficial repair of their cracking system using resin paste. The investigation comprises experimental results of 10 full scale beam-column joint specimens; five original joints and the corresponding retrofitted ones. The repair technique has been applied to RC joints with different joint reinforcement arrangements with minor to severe damage brought about by cyclic loading for the purposes of this work. Aiming at quantitative concluding remarks about the effectiveness of the repair technique, data concerning response loads, loading stiffness and energy absorption values have been acquired and commented upon. Furthermore, comparisons of damage index values and values of equivalent viscous damping, as obtained during the test of the original specimens, with the corresponding ones observed in the loading of the repaired ones have also been evaluated and commented. Based on these comparisons, it is deduced that the technique under investigation can be considered to be a rather satisfactory repair technique for joints with minor to moderate damage taking into account the rapid, convenient and easy-to-apply character of its application.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.1
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• pp.50.2-51
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• 2021

Stars form exclusively in cold and dense molecular clouds. To fully understand star formation processes, it is hence a key to investigate how molecular clouds form out of the surrounding diffuse atomic gas. With an aim of shedding light in the process of the atomic-to-molecular transition in the interstellar medium, we analyze Arecibo HI emission and absorption spectral pairs along with TRAO/PMO 12CO(1-0) emission spectra toward 58 lines of sight probing in and around molecular clouds in the solar neighborhood, i.e., Perseus, Taurus, and California. 12CO(1-0) is detected from 19 out of 58 lines of sight, and we report the physical properties of HI (e.g., central velocity, spin temperature, and column density) in the vicinity of CO. Our preliminary results show that the velocity difference between the cold HI (Cold Neutral Medium or CNM) and CO (median ~ 0.7 km/s) is on average more than a factor of two smaller than the velocity difference between the warm HI (Warm Neutral Medium or WNM) and CO (median ~ 1.7 km/s). In addition, we find that the CNM tends to become colder (median spin temperature ~ 43 K) and abundant (median CNM fraction ~ 0.55) as it gets closer to CO. These results hints at the evolution of the CNM in the vicinity of CO, implying a close association between the CNM and molecular gas. Finally, in order to examine the role of HI in the formation of molecular gas, we compare the observed CNM properties to the theoretical model by Bialy & Sternberg (2016), where the HI column density for the HI-to-H2 transition point is predicted as a function of density, metallicity, and UV radiation field. Our comparison shows that while the model reproduces the observations reasonably well on average, the observed CNM components with high column densities are much denser than the model prediction. Several sources of this discrepancy, e.g., missing physical and chemical ingredients in the model such as the multi-phase ISM, non-equilibrium chemistry, and turbulence, will be discussed.