• Development and Reproduction
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• v.10 no.2
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• pp.115-125
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• 2006

The present experiment was performed to confirm the effects of selenium on maturation and viability of mouse oocyte. Maturation of oocytes was observed by microscope, Germinal vesicle breakdown(GVBD) and polar body formation(PB) were confirmed at 2.5, 13 hours after in vitro culture. Viability of oocytes was observed by microscope. Normal and abnormal oocytes were distinguished by morphological change in vitro culture for 72 hours. Glutathione(GSH) content of collected oocytes from individual stage also was measured by glutathione assay using spectrophotometer. The results obtained were as follows; The low concentration of selenium($0.005\;{\mu}g/mL{\sim}0.5\;{\mu}g/mL$) increased the maturation rate of germinal vesicle(GV) oocytes to GVBD and PB oocytes. The high concentration of selenium($5\;{\mu}g/mL$) decreased the maturation rate. The low concentration of selenium increased the viability rate of PB oocytes. The high concentration of selenium did not affect the viability rate. The low concentration of selenium increased the GSH content in PB oocytes. The high concentration of selenium decreased GSH content. GSH content in PB oocyte was much higher than that in GVBD oocyte. The results indicate that the low concentration of selenium increases the maturation rate by helping quality elevation of oocyte and minimizing damages of oxidative stress generated from metabolism process. The low concentration of selenium also increases the viability rate by increasing GSH content.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.229-229
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• 2017

This research study was conducted to analyze the characteristics of different rice cultivars in abnormal temperature conditions (low temperature) for ripening period abnormalities, and to investigate the physiological causes behind the abnormalities. Four Korean high quality japonica-type rice cultivars, Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), Hwawang (HW) were used in the experiment. The following day after flowering, they were then moved into two phytotrons under natural daylight with 65% RH but controlled at different temperatures - one at $19/29^{\circ}C$ (night/day) and the other at $13/23^{\circ}C$ as the low - temperature study on ripening. For the cultivars at $13/23^{\circ}C$ (low temperature study), JB and JJ had a ripening rate of 93% which is similar to the ripening rates of cultivars at $19/29^{\circ}C$ at 45 days after heading (DAH). In contrast, GY and HW recorded lower ripening rates of 86% and 57% respectively. However, when the cultivars at $13/23^{\circ}C$ were harvested at 61 DAH (when the accumulated temperature reached $1100^{\circ}C$), the difference in ripening rates compared to the 4 cultivars of $19/29^{\circ}C$ harvested at 45 DAH was not obvious (JB 94%, JJ 97%, GY 97%, HW 88%). Starch content showed little difference among the 4 cultivars at different temperature conditions while amylose content was higher for cultivars at $13/23^{\circ}C$ compared to those at $19/29^{\circ}C$. In addition, the enzyme activities of starch biosynthesis were about 5~10 days slower in cultivars at $13/23^{\circ}C$ compared to cultivars at $19/29^{\circ}C$. The grain-filling rate showed highly significant correlations with the enzyme activities of Sucrose synthase ($R^2=0.70^{***}$), ADP glucose pyrophosphorylase ($R^2=0.63^{***}$), UDP glucose pyrophosphorylase ($R^2=0.36^{***}$), Starch synthase ($R^2=0.51^{***}$), and Starch branching enzyme ($R^2=0.59^{***}$). Among the enzymes, Sucrose synthase activity had the highest correlation coefficient with grain-filling rate. In conclusion, the activity of enzymes such as Sucrose synthase, UDP glucose pyrophosphorylase, ADP glucose pyrophosphorylase, Starch synthase, Starch branching enzyme in starch biosynthesis is proven to be highly related to the grain filling process. Notably, the decrease in the activity of Sucrose synthase and Starch branching enzyme and the late increase in ADP glucose pyrophosphorylase activity at low temperature in the ripening stage are considered to be disadvantageous as they delay ripening and increased amylose content.

• Journal of Korean society of Dental Hygiene
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• v.9 no.4
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• pp.593-605
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• 2009

Objectives : In an aging society, as the necessity of the elderly oral health care was increased, the oral health dimensions was a lot more visible important to a community public health service for the quality of life improvement. In oral health care of the elderly, the periodic scaling treatment was required to manage periodontal tissue care. Methods : So, the 319 elderly people were selected by a random sampling method, those who are visitors of 5 public health centers in the South Jeolla Province. based on the findings of personal interview questionnaires and oral health states from these elderly subjects, we made a comparative analysis of oral health states of the elderly scaling program subjects. Results : 1. Among the participants, 52.4% of the elderly benefited from scaling care project otherwise 47.6%, the subjects with periodontal diseases were 78.4%, whereas 21.6% of the ones who don't. 2. In regard to perceptions of oral cavity abnormal symptoms, findings revealed that the teeth smart sensation with something cold was 'yes' 62.7%, 'No' 37.3%, gingival bleeding was 'yes' 61.4%, 'No' 38.6%, oral odor(halitosis) was 'yes' 63.3%, 'No' 36.7%, and dried mouth was 'yes' 63.3%, 'No' 36.7%. 3. The study data showed 73.2% of periodontal disease subjects, and 24.6% of no periodontal diseases responded that they have hyperesthesia and 67.6% of periodontal diseases, 39.1% no periodontal diseases responded that they have gingival bleeding. 4. In comparison of the presence of periodontal disease with scaling service program state, it is show that the elderly scaling service program was significant statistically in Elderly's periodontal disease prevention. according to analysis, 52.4% of the subjects with and 34.8% of no periodontal diseases received the Elderly scaling service program(p<0.05). Conclusions : Therefore, oral health care of the elderly, a community public health service the periodic scaling treatment was required to manage periodontal tissue care.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1417-1425
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• 2014

The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.5993-5999
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• 2015

Background: Molecular testing for human papillomavirus (HPV) is the most objective and reproducible of all cervical cancer screening tests and also less demanding in terms of training and quality assurance. However, there is an impending need for cost effective molecular HPV testing methods with sampling ease, easy storage measures and minimum turn around times suitable for a low resource setting. Objective : Our aim was to evaluate the feasibility of using a fast transfer analysis (FTA) mini elute cartridge for cervical sampling to identify high risk HPV by real time PCR and to compare molecular HPV testing and Pap cytology testing to predict histologically confirmed cervical precancer (CIN 2+ lesions) in a cervical cancer prevention program. Materials and Methods: This was conducted as a pilot study (n=200) on women sampled using FTA mini elute cartridges, genotyped by two different real time PCR assays, detecting 13 high risk HPV (HR HPV) species, including HPV16 along with its physical DNA status. Results obtained from each of the tests were compared and analysed using suitable statistical tests. Results: With FTA mini elute cartridge samples HR HPV positivity was seen in 48/200 (24%). Of these, presence of HPV 16 DNA was observed in 28/48 (58.3%) women. High risk HPV was positive in 20% (37/185) of women with benign cytology and 73.3% (11/15) of women with abnormal cytology findings. A very significant correlation (${\chi}^2=22.090$ ; p=0.000) was observed between cytology and HR HPV findings showing an increasing trend of HR HPV prevalence in 50% (1/2) of LSIL, 75% (3/4) of HSIL and 100% (3/3) of SCC. Of the CIN 2+ lesions identified by histopathology, 88.9% (8/9) had HR HPV. A significant association (${\chi}^2=11.223$ ; p=0.001) of HR HPV and histopathologically confirmed CIN 2+ lesions was found. Sensitivity of the two tests were comparable but specificity of Pap testing was better (90.7% vs 70.4%) to predict histopathologically diagnosed cervical precancers. Conclusions: The current study explored the feasibility of using a FTA mini elute cartridge for cervical sampling for the first time in India as a part of a community based cervical cancer prevention program. We suggest that FTA based sampling is suitable and feasible for real time based HPV testing. Molecular HR HPV testing can be more sensitive and useful to identify high risk women requiring Pap testing which is more specific to detect histologically confirmed cervical precancer.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.341-349
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• 2014

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

• Economic and Environmental Geology
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• v.50 no.5
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• pp.401-414
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• 2017

To prevent the damages from earthquakes, various researches have been conducted around the world focusing on earthquake prediction and forecasting for several decades. Among various precursory phenomena, changes in groundwater level and quality are considered to be reliable for estimating the time of earthquake occurrence and its magnitude. In effects, some countries impacted by frequent earthquakes have established and operated the groundwater monitoring network for earthquake surveillance and prediction. In Korea, recently researches have begun for using groundwater monitoring techniques for earthquake prediction. In this paper, the groundwater monitoring networks of China, Japan, and the United States were reviewed focusing on the facilities and results of researches to deduce the tasks for earthquake prediction researches using groundwater monitoring techniques in Korea. In results, research needs are suggested in the implementation of groundwater monitoring networks for specifically earthquake surveillance with the real-time monitoring and the measures to quantify the degrees of abnormal changes in the relationship of distance from the earthquake epicenter.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.60-64
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• 2009

Effects of $CO_2$ concentration (500, 800, 1,000 and 1,500 ppm) on the initiation and development of fruit body of Grifola frondosa on sawdust cultivation were studied. Optimum concentrations of carbon dioxide for the initiation and development of the fruit body showed the ranges from 500 to 800 ppm. Fruit body initiation was accelerated at lower than 800 ppm $CO_2$ exposure but the maturing of the fruit body was not influenced by above treated $CO_2$ concentrations. The higher ratio of primordial formation, faster fruit body initiation and higher yield were obtained at below 800 ppm of $CO_2$ level, whereas over 1,000 ppm of $CO_2$ levels showed abnormal and lower quality of fruiting bodies. Based on the above results, it is concluded that the favorable $CO_2$ level for bag culture of G. frondosa was below 800 ppm.

• Journal of Korean Public Health Nursing
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• v.2 no.2
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• pp.5-20
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• 1988

This study is a series of reviews in connection with the application of the Questionnaire THI, in order to intend to provide such datum to be of a help as the screening-test by means of getting through comparative analysis, and finding out existence of any disease appeared as a result of the general medical examinations, and the psychosomatic symptoms, through the questionnaire THI. This study had been researched through the 507 insureds of the life insurance residing in Seoul and in the suburbs of Seoul, during the period 5 Jan. 1987 to 27 Feb. through the questionnaires. Exception of the 88 insincere respondents out of the total objectives, the 419 questionnaires were nanlized through percentage, Mean, T-test, ANOVA test, and Discriminant Analysis. 1. The Subjective Health Problem of the Objectives: The average of the health problem appliation quorum appeared to be 1. 81, the articles of both nervousness and aggressiveness appeared to be each 2.25 and 2.04. It tells that the state of appliation is heavy. It was very significant statistically the relation between the elements of sex, ages, educational backgrounds and occupations. 2. The result of the General Examination of the Objectives: The number of the persons with abnormal findings was 300 persons among the total of 419 persons as a result of the general medical examinations, and it was applicable to $73.5\%$. By each age and marital state were statistically significant. 3. Comparison of the result of the General Examination and Subjective Health of those Objectives: As a result of discriminant analysis of the independent variables through the result of the general examination, the greatest element for influence appeared to be the age, and then, regular examination, sex, and the somatic complaints of the THI in order. Accordingly, it is considered to be able to function as the screening test of the general examination, the THI. In case of a greater unit of group required a medical examination, It is considered to be of a help for the health management service for more effective and precise in quality and economic, only if a medical examination is carried out after selecting the somatic subjective problem and research in advance to the application of the THI prior to the examination.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.213-221
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• 2018

We analyzed the skin coloration and anthocyanin composition of 'Kyoho' and 'Heukboseok' grape berries to determine the cause of poor coloring in 'Kyoho' berry skins under high temperature (HT) at veraison. Although the skin coloration inhibited in both 'Kyoho' and 'Heukboseok' berries under HT for 30 days from veraison, the total anthocyanin content in 'Heukboseok' berry skins increased to the level of control after the end of temperature treatment, but 'Kyoho' did not increase. Malvidin derivatives were most significantly reduced in 'Kyoho' berry skins, followed by those of delphinidin and petunidin. Among individual anthocyanins, diglucosides and acylated malvidin derivatives were most decreased in 'Kyoho' berry skins. Acylated and tri-hydroxylated anthocyanins were reduced more than those of non-acylated and dihydroxylated, respectively. All different types of anthocyanin components in 'Kyoho' berry skins decreased by HT, and they were similar to that of total anthocyanin. In 'Heukboseok' berry skins, accumulations of all different types of anthocyanins were inhibited by HT, and increased to the level of control after the end of the treatment. These results suggest that the poor coloration of 'Kyoho' under HT at veraison was not caused by the decrease of specific anthocyanins but because the whole anthocyanin biosynthesis was suppressed by HT.