• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.18-24
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• 2019

Isoflavones, a group of secondary metabolites present in plants and especially abundant in soybeans, are garnering increasing interest because of their medicinal properties. Numerous environmental factors influence isoflavone accumulation in plants. Successful attempts to increase isoflavone contents in soybean seeds have not been widely reported. In this report, we examined an effective method for producing a high amount of isoflavones during soybean seed germination, by treating the seed with 4 abiotic factors. Soybean seeds were exposed to ultrasound (50 Hz) and heat ($40^{\circ}C$) for 30minutes, then germinated for 5 days in darkness. Another sample was subjected to water deficit during germination in darkness, and the other sample was subjected to light conditions during the germination period. The results showed that the ultrasound treatment increased isoflavone content during soybean germination. Three days after germination, the ultrasound-treated seeds presented the highest isoflavone content (6,042.1 ug/g dry weight) compared to the other treatments, which was also 1.3-fold increases over the content of the raw seed's before germination. With respect to each type of isoflavone group, aglycone (partially genistein) content was markedly higher, whereas glucoside was lower than that of the untreated samples. In other experiment, total isoflavone content after heat, water deficit, and light treatments was 5,600.0, 4,740.1, and 5,631.4 ug/g, respectively. These present work suggests that ultrasound treatment is a novel approach for improving the production of isoflavones in soybean seeds during germination.

• Journal of Wetlands Research
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• v.12 no.3
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• pp.21-37
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• 2010

The abiotic factors and the vegetation naturalness of the 13 tributary wetlands around Namgang-River were assessed to investigate the present state and to present the conservation counter plan for the wetland ecosystem of the tributaries. Assessment indices for the abiotic factors were estimated based on the dominant land use types of the riparian zone, ecological function of the upper levee, levee slope structure, artificiality and utilization intensity of the waterfront, artificiality of the revetment structure, diversity of the substrate, and severance of the transverse. The assessment index of Omi-cheon is the highest among 13 tributaries. The second was Banseong-cheon and third was Hyangyang-cheon, followed by Nabul-cheon and Yeongcheon-gang and Sugok-cheon, Jungchon- cheon, and Daegok-cheon in their order and Munsan-cheon got the lowest assessment index. Assessment indices for the vegetation naturalness were estimated based on the vegetation diversity, exotic species dominance, annual herb dominance, naturalness and peculiarity of the vegetation, and species diversity. The assessment index of Omi-cheon is the highest among 13 tributaries. The second was Sugok-cheon and third was Banseong-cheon, followed by Yeongcheon-gang, Jungchon- cheon, Jinae-cheon, Nabul-cheon, and Jisu-cheon in their order, and Yonga-cheon got the lowest assessment index. The grades of the stream naturalness were estimated based on the the naturalness indices for the abiotic factors and the vegetation naturalness. The grades of Omi-cheon is the highest among 13 tributaries as the grade I. Those of Banseong-cheon, Sugok-cheon, Yeongcheon-gang, Nabul-cheon and Jungchon-cheon, Hyangyang-cheon, Jinae-cheon, Jisu-cheon, Daegok-cheone, and Munsan-cheon and Doksan-cheon were grade II in their order, and Yonga-cheon got the lowest as the grade III. It was suggested that restoration of the simple and flat substrate, create the natural vegetation on the levee slope constructed with concrete or stone wall, and rehabilitation of the eco-bridge were demanded to improve the grades of the stream naturalness through the restoration of the tributaries for the diverse aquatic wildlife, high vegetation diversity and species diversity with the vegetation consisted of perennial herbs and trees.

• Journal of Ecology and Environment
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• v.38 no.1
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• pp.1-14
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• 2015

Whilst it is clear that increasing temperatures from global environmental change will impact the positions of alpine treelines, it is likely that a range of regional and local scaled factors will mediate the overall impact of global scale climate drivers. We summarized 12 categories of abiotic and biotic factors as 4 groups determining treeline positions. First, there are global factors related to climate-induced growth limitation and carbon limitation. Second, there are seven regional and local factors related to treeline dynamics including frost stress, topography, water stress, snow, wind, fire and non-fire disturbance. Third, species-specific factors can control treeline dynamics through their influence on reproduction and life history traits. Fourth, there are positive feedbacks in structuring the dynamics of treelines. Globally, the commonly accepted growth limitation hypothesis is that growth at a treeline is limited by temperature. Meanwhile, positive feedbacks between canopy cover and tree establishment are likely to control the spatial pattern and temporal dynamics of many treelines. The presence of non-linear dynamics at treelines has implications for the use of treelines as barometers of climate change because the lagged responses and abrupt shifts inherent in non-equilibrium systems may combine to mask the overall climate trend.

• Journal of Environmental Science International
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• v.12 no.12
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• pp.1215-1225
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• 2003

The purpose of this research was to investigate the characteristics of urban ecotopes and to classify ecotopes systematically from them. Total of 15 characteristics for classification of ecotopes were selected, and there were categorized 3 factors, that is abiotic, biotic and anthropological factors. The ecotope types in the study area were classified into 67. The classification of ecotope was made with SPSS for Windows Version 10.0 on the basis of the 15 characteristics. As the results of cluster analysis using the average linkage method between groups, groups of ecotope type were divided into 15 clusters. It was known that there was not a great difference in an affinity as the result of overlapping the maps of ecotope type and land use type. This research suggested characteristics for classification of ecotopes, but there was a limit to Set the objective method for grade classification because of lacking in the basic data, the research of characteristics will be accomplished continuously.

• Journal of Wetlands Research
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• v.24 no.4
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• pp.354-366
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• 2022

To analyze the distribution of plant communities growing in river wetlands and the relationship between biotic and abiotic environmental factors, plant communities and environmental factors were investigated in river wetlands in the Boseong River. The Boseong River Wetland, the research site, consists of Hwapyeong Wetland, Bangujeong Wetland, and Seokgok Wetland. From June to September 2022, a plant community survey was conducted from the perspective of physiognomical vegetation, and the coverage of the emerging species followed the Braun-Blanquet scale. Plant species and the coverage of each species were recorded in the quadrant for plant community survey, and the cover of the quadrant, the total number of species, and the number of exotic species were measured as biological factors. As abiotic factors, altitude, orientation, inclination, soil texture, litter layer depth, dominant species diameter at breast height, and topography were recorded. In a total of 50 square plots, the most common Salix koreensis and Phragmites japonicus communities were found, and the community with the highest Shannon species diversity index was Phragmites japonicus-Echinochloa caudata community. As a result of ordination analysis by DCCA, the most significant clusters were separated according to topographic factors such as leeve, leeve slope, upper floodplain, lower floodplain, upper waterside, middle waterside, lower waterside, river island and opem water. As rare plants that need to be preserved in river wetlands, Hydrocharis dubia and Penthorum chinense were found in lower waterside, and it was found that the management of the river in the reservoir is necessary in line with the topographical distribution of ecosystem-disrupting plants, such as Paspalum distichum var. indutum.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.1
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• pp.104-115
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• 2015

This study was conducted during April-June in 2008 and 2010, following the Taean Oil Spill. We measured year-to-year changes in benthos species abundances and composition in 16 intertidal zone regions of Chungcheongnam-do. In total, 154 species were found 2008 and 134 in 2010; the mean abundance per area was $403ind./m^2$ and $242ind./m^2$, respectively. In 2008, the 10 dominant species included four species of arthropods, three species of annelids, and three species of mollusks. In 2010, dominant species included five species of annelids, three species of mollusks, one species of arthropods, and one other species. We used bray-curtis similarity to group species and found two groups in 2008 and five in 2010, complementing our NMDS analysis. Finally, we tested correlations between abiotic and biotic factors, and implemented a BIO-ENV analysis, which showed that sediment type, MZ (Phi), and organic content are important environmental factors affecting benthos in the Chung-nam tidal flats.

• Ocean Science Journal
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• v.42 no.2
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• pp.69-79
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• 2007

A vertical transect with 4 km length was established for the macrofaunal survey on the Chokchon macrotidal flat in Kyeonggi Bay, Incheon, Korea, 1994. Tidal elevation (m) and sediment mean grain size $(\phi)$ were inversely predicted by the transfer functions from the faunal assemblages. Three methods: weighted average using optimum value (WA), tolerance weighted version of the weighted average (WAT) and maximum likelihood calibration (MLC) were employed. Estimates of tidal elevation and mean grain size obtained by using the three different methods showed positively corresponding trends with the observations. The estimates of MLC were found to have the minimum value of sum of squares due to errors (SSE). When applied to the previous data $(1990\sim1992)$, each of three inference models exhibited high predictive power. This result implied there are visible relationships between species composition and faunas' critical environmental factors. Although a potential significance of the two major abiotic factors was re-affirmed, a weak tendency of biological interaction was detected from faunal distribution patterns across the flat. In comparison to the spatial and temporal patterns of the estimates, it was suggested that sediment characteristics were the primary factors regulating the distribution of macrofaunal assemblages, rather than tidal elevation, and the species composition may be sensitively determined by minute changes in substratum properties on a tidal flat.

• Molecules and Cells
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• v.40 no.10
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• pp.697-705
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• 2017

The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

• Korean Journal of Microbiology
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• v.22 no.3
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• pp.157-165
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• 1984

Seasonal and spatial variations in propagule numbers of Trichoderma species were investigated every other month for one year in deciduous and coniferous forest soils and evaluated the relationships of Trichoderma spp. populations to soil environmental factors. The total population of Trichoderma spp. increased until summer and then declined until winter. The yearly mean frequency of Trichoderma spp. exceeded 1.4% of total fungal propagules in two sites. Decreases of absolute an relative propagule numbers of Trichoderma spp. with increasing soil depth were found and variation in Trichoderma spp. propagules caused by differences in soil depth ($0{\sim}50cm$) was greater than that caused by differences in sampling time. The most common species occurring in two sites was T. viride, followed by T. polysporum, T. koningii, and T. hamatum. Individual species of Trichoderma showed diferent abundance trend in accordance with sampling time. T. viride was dorminant from spring to autumn, while T. polysporum dominated over the other speicies in winter. Variations in propagule number of Trichoderma sppp. were principally mediated by the actions of biotic environmental factors rather than by the direct effects of abiotic factors. In multiple-regression analyses, 48% of the total vaiation in Trichoderma spp. propagules in deciduous site could be accounted for by total fungal propagules and soil CMCase actvity. In coniferous site, 65% of total variation could be accounted for by total fungal and bacterial propagules, moisture content and organic carbon content.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.275-281
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• 2013

Low temperature is one of the major environmental factors that affect productivity including reduced growth and budding of vines, and changes of metabolic processes in grape (Vitis spp.). To screen the specific expression of abiotic stress-related genes against cold treatment in 'Kyoho' and 'Campbell Early' grapevines, expression of various defense-related genes was investigated by RT-PCR and real-time PCR. Among the 67 genes analyzed by RT-PCR and real-time PCR, 17 and 16 types of cDNA were up-regulated, while 5 and 6 types were down-regulated in cold-treated 'Kyoho' and 'Campbell Early' grapevines, respectively. Genes encoding carotene (Cart3564 and Cart4472), chalcone isomerase (CHI), cytochrome P450 (CYP), flavonol synthase (FLS), endo-${\beta}$-glucanase precursor (Glu), glutathione peroxidase (GPX), glutathione-S-transferase (GST), leucine-rich repeats (LRR), manganese superoxide dismutase (Mn-SOD), phenylalanine ammonia lyase (PAL), polygalacturonase-inhibiting protein (PGIP), proline rich protein 2 (PRP2), small heat shock protein (sHSP), temperature induced lipocalin (TIL), and thaumatin-like protein (TLP) were up-regulated, while those encoding CBF like transcription factor (CBF1), chitinase-like protein (CLP), cold induced protein (CIP), glycerol-3-phosphate acyltransferase (GPAT), and mitogen-activated protein kinase (MAPK) were down-regulated by low temperature treatment in both in 'Kyoho' and 'Campbell Early'.