• Microbiology and Biotechnology Letters
• /
• v.48 no.3
• /
• pp.322-327
• /
• 2020

Abortive infection systems (Abi) are phage resistance systems that can be prophage-encoded. Here, two genes encoding an Abi system were identified on a prophage sequence contained by the chromosome of the Levilactobacillus brevis strain UCCLBBS124. This Abi system is similar to the two-component AbiL system encoded by Lactococcus lactis biovar. diacetylactis LD10-1. The UCCLBBS124 prophage-derived Abi system (designated here as AbiL₁₂₄) was shown to exhibit specific activity against phages infecting L. brevis and L. lactis strains. Expression of the AbiL₁₂₄ system was shown to cause reduction in the efficiency of plaquing and cell lysis delay for phages of both species.

• Journal of the Korean Society of Safety
• /
• v.37 no.3
• /
• pp.45-51
• /
• 2022

This paper focuses on a recently proposed asymmetric base-isolation coupling control system (ABiCS) for the vibration control of adjacent twin buildings. The ABiCS consists of inter-story diagonal dampers, a connecting damper between the two buildings, and a seismic isolation device at the base floor of one building. To investigate the control characteristics of ABiCS, a parametric study was performed by numerically simulating the 20-story twin buildings. In the parametric study, the control capacities of the inter-story diagonal dampers, connecting damper, and seismic isolation device were considered as varying parameters. The parametric study results indicate that the connecting damper between the two buildings reduces the responses of both buildings only at optimal or near-optimal capacity. In addition, adjusting the stiffness of the base isolation is found to be the most effective method for improving seismic performance and achieving cost-effectiveness. Accordingly, we presented a scenario-based performance improvement approach in which reducing the stiffness of the base isolation device could be an effective technique to improve the seismic performance of both buildings. However, note that checking the maximum allowable displacement of the base isolation device is essential.

• Journal of Life Science
• /
• v.11 no.3
• /
• pp.279-283
• /
• 2001

Amyotrophic lateral sclerosis(ALS) is a progressive neurologic disorder resulting from the degeneration of upper and lower motor neurons, and is inherited in 10% of cases. About 20% of familial ALS, clinically indistinguishable from sporadic ALS, is caused by mutations of Cu/Zn superoxide dismutase on chromosome 21q22.21 inherited as an autosomal dominant trait. We now report a new locus in the non-SOD1 dominantly inherited ALS. We screened a large ALS family with 11 affected individuals and one obligate gene carrier with genome-wide ABI polymorphic markers using the ABI 377 automated system. No evidence of linkage was obtained with the autosomal markers. We next screened this family with X chromosome markers as there was no evidence of male-to-male tran-smission of the disease. Linkage was established with several X chromosome markers with a lod score up to 3.8; almost the maximum possible score in this family. Our finding imply that a gene for the dominant expression of a neuronal degeneration is coded on X chromosome and raise the question of the role of X-linked genes that escape inactivation in this pathogenesis. More importantly, our finding that a gene causing ALS is localized on X-chromosome has direct investigational relevance to sporadic ALS, where epidemiological studies show male gender predominance(1.3:1) and earlier onset in men by 5-10 years.

• Journal of Life Science
• /
• v.14 no.5
• /
• pp.840-846
• /
• 2004

A study for expression of Korean Mistletoe (KM) lectin gene (A,B) in Saccharomyces cerevisiae was done using transforming system of yeast. In order to overexpress the genes efficiently in yeast, two lectin genes (A,B) were re-cloned and modified including Kozak translation initiation sequence using PCR amplification. The constructed plasmids containing modified lectin A and B genes were transformed to S. cerevisea INVSc (MAT G, his3 $\Delta$1, leu2, trpl-289, ura3-52). The transformed cells were identified by DNA sequencing with ABI3700 system and induced with 2% of galactose for recombinant KM lectin (rKM lectin) protein. The rKM lectin A and B proteins were determinated about 29kDa size of protein by SOS-P AGE and western blotting analysis. The expressed recombinant lectin was determinated 1.24∼1.75 $\mu\textrm{g}$ per 1 mg of cytosolic soluble protein by sandwich ELISA method. Moreover the lectin genes were expressed as maximum level at 36 h after galactose induction and lectin A gene was were repressed after 48 h.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2021.05a
• /
• pp.305-307
• /
• 2021

군사 분야에서 사용하는 기존 영상융합체계는 영상에서 미확인 개체를 식별하는 Activity-Based Intelligence(ABI) 기술과 객체들에 대한 지식정보를 관리하는 Structured Observation Management(SOM) 기술을 연동하여 다양한 관점에서 분석하고 있다. 그러나 군사적인 목적을 달성하기 위해서는 미래 정보가 중요하기 때문에 주변 맥락 정보를 통합하여 분석해야 할 필요성이 있으며 이를 위해 주변맥락 정보를 분석하는 딥러닝 모델 적용이 필요하다. 본 논문에서는 딥러닝 모델 기반 영상 판독 시스템 구축을 하기 위한 전처리 과정을 설계하였다. pyhwp 라이브러리를 이용하여 영상 정보 판독 데이터를 파싱 및 전처리를 진행하여 데이터 구축을 진행하였다.

• Korean Journal of Remote Sensing
• /
• v.34 no.6_1
• /
• pp.925-939
• /
• 2018

Absorbed shortwave radiation at the surface is an important component of energy analysis among the atmosphere, land, and ocean. In this study, the absorbed shortwave radiation was calculated using a radiation model and surface broadband albedo data for application to Geostationary Earth Orbit Korea Multi-Purpose SATellite (GEO-KOMPSAT-2A; GK-2A). And the results (GWNU algorithm) were compared with CERES data and calculation results using pyranometer and MODIS (Moderate Resolution Imaging Spectroradiometer) data to be selected as the reference absorbed shortwave radiation. This GWNU algorithm was also compared with the physical and statistical algorithms of GOSE-R ABI and two algorithms (Li et al., 1993; Kim and Jeong, 2016) using regression equation. As a result, the absorbed shortwave radiation calculated by GWNU algorithm was more accurate than the values calculated by the other algorithms. However, if the problem about computing time and accuracy of albedo data arise when absorbed shortwave radiation is calculated by GWNU algorithm, then the empirical algorithms explained above should be used with GWNU algorithm.

• Wind and Structures
• /
• v.34 no.2
• /
• pp.231-257
• /
• 2022

Transmission lines systems are important components of the electrical power infrastructure. However, these systems are vulnerable to damage from high wind events such as hurricanes. This study presents the results from a 1:50 scale aeroelastic model of a multi-span transmission lines system subjected to simulated hurricane winds. The transmission lines system considered in this study consists of three lattice towers, four spans of conductors and two end-frames. The aeroelastic tests were conducted at the NSF NHERI Wall of Wind Experimental Facility (WOW EF) at the Florida International University (FIU). A horizontal distortion scaling technique was used in order to fit the entire model on the WOW turntable. The system was tested at various wind speeds ranging from 35 m/s to 78 m/s (equivalent full-scale speeds) for varying wind directions. A system identification (SID) technique was used to evaluate experimental-based along-wind aerodynamic damping coefficients and compare with their theoretical counterparts. Comparisons were done for two aeroelastic models: (i) a self-supported lattice tower, and (ii) a multi-span transmission lines system. A buffeting analysis was conducted to estimate the response of the conductors and compare it to measured experimental values. The responses of the single lattice tower and the multi-span transmission lines system were compared. The coupling effects seem to drastically change the aerodynamic damping of the system, compared to the single lattice tower case. The estimation of the drag forces on the conductors are in good agreement with their experimental counterparts. The incorporation of the change in turbulence intensity along the height of the towers appears to better estimate the response of the transmission tower, in comparison with previous methods which assumed constant turbulence intensity. Dynamic amplification factors and gust effect factors were computed, and comparisons were made with code specific values. The resonance contribution is shown to reach a maximum of 18% and 30% of the peak response of the stand-alone tower and entire system, respectively.

• IMMUNE NETWORK
• /
• v.6 no.2
• /
• pp.93-101
• /
• 2006

Background: Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that naive Ag-specific $CD4^+$ T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization. Methods: To assess this hypothesis, the response kinetics of DO11.10 TCR $CD4^+$ T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice. Results: Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide $(OVA_{323-339})$, whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low $OVA_{323-339}$ peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group. Conclusion: These results suggest that primary stimulation with a low dose of Ag leads to better memory $CD4^+$ T cell generation after secondary immunization. Therefore, these facts imply that optimally primed $CD4^+$ T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination.

• Biomedical Science Letters
• /
• v.16 no.3
• /
• pp.193-196
• /
• 2010

Multiplex PCR-based short tandem repeat (STR) analysis is considered as a good tool for monitoring bone marrow engraftment after sex-mismatched allogeneic transplantation and provides a sensitive and accurate assessment of the contribution of both donor and/or recipient cells in post-transplantation specimens. Forensic STR analysis and quantitative real time PCR are used to determine the proportion of donor versus recipient each contained within the total DNA. The STR markers were co-amplified in a single reaction by using commercial $PowerPlex^{(R)}$ 16 system and $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ / $Yfiler^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI $PRIS^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The $GeneMapper^{TM}$ ID software were used for size calling and analysis of STR profiles. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA / Y Human Male DNA Quantification Kit The intent of this study was to analyze the ratio of donor versus recipient cells in the post-transplant peripheral blood, spleen, lung and kidney specimens. Specimens were taken from the traffic accident male victim who had been engrafted from bone marrow female donor. Blood and spleen specimens displayed female donor DNA profile. Kidney specimen showed male recipient DNA profile. Interestingly, lung tissue showed mixed profiles. The findings of this study indicate that the forensic STR analysis using fluorescence labeling PCR combined with capillary electrophoresis is quick and reliable enough to assess the ratio of donor versus recipient cells and to monitor the mixed chimeric patterns.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.1
• /
• pp.299-302
• /
• 2013

Methylenetetrahydrofolate reductase (MTHFR) has been reported to be associated with DNA methylation, an epigenetic feature frequently found in gastric cancer. We conducted a case-control study to explore the association of MTHFR C677T polymorphisms with gastric cancer risk and its relation with the DNA methylation of COX-2, MGMT, and hMLH1 genes. Genotyping of P16, MGMT and HMLH1 was determined by methylation-specific PCR after sodium bisulfate modification of DNA, and genotyping of MTHFR C677T was conducted by TaqMan assays using the ABI Prism 7911HT Sequence Detection System. Folate intake was calculated with the aid of a questionnaire. Compared with the MTHFR 677CC genotype, the TT genotype was significantly associated with 2.08 fold risk of gastric cancer when adjusting for potential risk factors. Individuals who had an intake of folate above $310{\mu}g$/day showed protective effects against gastric cancer risk. The effect of MTHFR C677T polymorphisms on the risk of gastric cancer was modified by folate intake and methylation status of MGMT (P for interaction <0.05).