• The Korean Journal of Malacology
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• v.25 no.2
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• pp.121-126
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• 2009

This study was performed to determine the effect of temperature condition on growth of juvenile abalone Haliotis discus hannai with the different feeds. Two types of feed (dry sea tangle and experimental diet) at water temperatures of 20 and $23^{\circ}C$, and 23 and $26^{\circ}C$ were applied to experimental abalone twice. Forty-five juvenile abalone averaging 10.1 g in the first feeding trial and 11.5 g in the second feeding trial were randomly stocked into 6 of 50 L plastic rectangular containers each. Fishmeal, soybean meal and shrimp head meal was used as the primary protein source in the experimental diet. And dextrin and wheat flour, and soybean oil were used as the primary carbohydrate and lipid sources in the experimental diet, respectively. The dry sea tangle and experimental diet were fed to abalone once a day at the ratio of 1.5-2.0% total biomass of abalone with a little leftover in each experimental condition. Weight gain of abalone was significantly affected by feed type, but not by water temperature in the first feeding trial. Regardless of water temperature, weight gain of abalone fed the experimental diet was significantly higher than that of abalone fed the dry sea tangle. However, weight gain of abalone was significantly affected by water temperature, but not by feed type in the second feeding trial. Weight gain was highest in abalone fed the experimental diet at $23^{\circ}C$, followed by abalone fed the dry sea tangle at $23^{\circ}C$, abalone fed the experimental diet and dry sea tangle at $26^{\circ}C$, which was lowest. Moisture and crude protein content of the edible portion of abalone was significantly affected by feed type, but not by water temperature in the second feeding trial. However, ash content of the edible portion of abalone was significantly affected by water temperature, but not by feed type. In considering these results, it can be concluded that the well formulated feed was superior to the dry sea tangle for growth of juvenile abalone, and water temperature conditions of 20 and $23^{\circ}C$ seemed to be better than $26^{\circ}C$ to improve weight gain of abalone.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.3
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• pp.223-231
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• 2020

Karyotype analysis is a major work in the process of triploid abalone production for the purpose of productivity and quality improvement. However, the metaphase spreads for karyotype analysis have been prepared just from the larvae at trochophore stage, which has restricted the spectrum of sample correction inhibiting more efficient analysis. Here, we investigated the feasibility of preparing metaphase spreads from the larvae at veliger stage that is the next developmental stage of trochophore. For this, diploid and triploid larvae at trochophore and veliger stages from Pacific abalone (Haliotis discus hannai) were subjected to metaphase spread preparation and its efficiencies were measured and compared each other. As the results, although the efficiencies of metaphase spread preparation were significantly lower in the larvae at veliger stage compared to the ones at trochophore stage regardless of ploidy status, we found that the preparation of metaphase spreads, which showed the clear chromosomal images containing the normal number of chromosomes, was possible from the veliger stage larvae. On the other hands, all larvae used in this study regardless of developmental stage and ploidy did not show colchicine sensitivity. Moreover, no significant difference was observed in cell cycle distribution of the cells comprising larvae between two developmental stages regardless of ploidy status. These suggested that the details of protocol to prepare metaphase spreads from abalone larvae should be optimized depending on its developmental stages. Taken together, we demonstrated the feasibility of preparing metaphase spreads from H. discus hannai veliger stage larvae for karyotype analysis.

• Journal of Aquaculture
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• v.8 no.3
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• pp.159-170
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• 1995

Triploid abalone, Haliotis discus hannai, was induced by the cold $(0^{\circ}C\;and\;3^{\circ}C)$ or the heat $(35^{\circ}C\;and\;40^{\circ}C)$ shock procedure with fertilized eggs, 12 min. or 32 min. post fertilization with the various time intervals of shock duration. Fertilization rate of each experimental group was not significantly different from that of corresponding diploid control (P>0.05). However hatching rates and normality rates of triploid larvae were significantly different from those of corresponding diploid control (P <0.05). In heat shock groups at $40^{\circ}C$, fertilization rate of eggs was extremely low $(0\~2.7\%)$ and hatched larvae were not detected in these treatment groups. Incidence of triploidy was confirmed by chromosome count and the highest rates of triploid $(84.0\%)$ revealed in cold $(3^{\circ}C)$ shock with 15 min. treatment duration 12 min. after fertilization. The number of diploid chromosome was 2n: 36, and that of tiploid was 3n=54.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.544-550
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• 2020

This study was conducted to estimate the genetic improvements by selection criteria using the genetic parameters and breeding values for population of abalone Haliotis discus hannai. Genetic parameters and breeding values were estimated using all measurement data of growth traits (shell length, shell width and total weight) at 18 and 30 months old after artificial fertilization for 3,029 individuals produced in April 2014. Growth traits all exhibited moderate heritability (0.253-0.354). So it is considered that family selection will be more advantageous than individual selection. It was found that a higher genetic improvement could be expected when selecting the top 10% based on the breeding values of total weight rather than other traits. In particular, a higher genetic improvement could be expected when selecting the top 10% at 30 months old than 18 months old after artificial fertilization. This seems to be because the selection differential and heritability were higher at the 30 months old. Therefore, by estimating genetic parameters and breeding values of a population for production of the next generations by stage of growth, if they are used properly in selection and mating according to the improvement direction, it is considered that more breeding effects can be expected.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.2
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• pp.108-112
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• 2007

A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

• Journal of Aquaculture
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• v.16 no.4
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• pp.257-261
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• 2003

The effects of different stocking densities on the growth of juvenile abalone, Haliotis discus hannai and water quality in the recirculating system with rotating biological contactor, were assessed. The trials were conducted using total weight 32 g juveniles abalone for 60 days at stocking densities of 5, 10, 15 kg/$m^2$. The animals were fed sufficient amounts of the pellet diet of 30.4% protein during the experimental period. Daily feeding rate (DFR) and survival rate (SR) of 5 kg/$m^2$ showed 0.76% and 85.5% those were not significantly differed 10 kg/$m^2$ showing 0.75% and 96.0% (P>0.05). DFR and SR of 15 kg/$m^2$ showing 0.38% and 31.2% were significantly lower than 5 and 10 kg/m2 (P<0.05). Daily growth rate (DGR) and feed efficiency (FE) showed the highest as 0.19% and 24.2% in 5 kg/$m^2$ the lowest as 0.05% and 14.3% in 15 kg/$m^2$ (P<0.05). According to, these results was an inverse relationship between growth and stocking density. The optimum density of juvenile abalone from this experiment is 5 to 10 kg/$m^2$ in the recirculating system.

• The Korean Journal of Malacology
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• v.30 no.3
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• pp.243-248
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• 2014

This study histologically describes the gonadal development and reproductive cycle of the abalone, Haliotis discus hannai inhabiting Jindo on the southern seashores of Korea. Gonads displayed histologically definitive seasonal changes. The female ratio (F/F + M) was 57% (n = 154/271). The gonad index (GI) of both males and females was the highest (3.3 and 3.9) in June and was the lowest (1.1 and 1.1) in December. The condition index (CI) and meat weight rate (MWR) were highest in May and lowest in October. The pattern of changes in the GI, CI and MWR were similar to the pattern of seasonal changes in gonadal tissues. The reproductive cycle was divided into an inactive stage (November-December), early active stage (January-March), late active stage (March-April), ripe stage (May-June) and spent and degenerative stage (July-October). The main spawning period of Haliotis discus hannai was July to August at Jindo in 2013.

• Journal of Aquaculture
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• v.13 no.1
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• pp.55-62
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• 2000

A 10-week feeding trial was conducted to investigate the effects of several yeasts with or without chemical treatment (protoplasted) in formulated diets on growth and body composition of juvenile abalone(Haliotis discus hanai). There replicate groups of the abalone average weighing 210 mg were fed one of eight isonitrogenous (30%) and isolipidic (4.4%) diets containing 3% Kluyveromyces fragilis protoplasted K. fragilis Candida utilis protoplasted C. utilis, Saccharomyces cerevisiae protoplasted S. cerevisiae or brewer's yeast. In addition these formulated diets were compared with commercial diet. Survival rate and proximate analysis of soft whole body of abalone were not significantly affected by the different dietary yeasts and commercial diet (P>0.05) Body weight gain and soft body weight control diet and diets containing S. cerevisiae or brewer's yeast (P<0.05) Shell length of abalone fed yeast and commercial diet (P<0.05) Th results suggest that protoplasted K. fragilis as an additive in this formulated diet can improve weight gain of abalone.

• The Korean Journal of Malacology
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• v.32 no.2
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• pp.103-109
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• 2016

The effect of different feeding rate on the growth and survival rate of 3-year-old abalone, Haliotis dicsus hannai were investigated in marine net cage culture. Feeding rate is determined that was fed 5, 10, 15 and 20 percentage to abalone at body weight once daily : 5 daily feeding rate (DFR), 10 DFR, 15 DFR and 20 DFR. After that, it was conducted to reared during thirteen month with two replicates in net cage culture. In the growth performance of reared abalone (initial mean shell length $73.77{\pm}11.27mm$) in net cage culture, that the absolute growth rate (ARG), daily growth rate (DGR) and specific growth rate (SGR) to the shell length and shell breadth, as well as weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to body weight of 5 DFR were higher than those of different groups (P < 0.05). Also, survival rate of all feeding rate groups was not significant, but it was showed bellow 30 percents. Therefore, these results is showed that the daily feeding rate for natural feed of 3-year-old abalones reared in net cage culture should be to supply among five to ten percents.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.570-575
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• 2001

Three feeding experiments were conducted to investigate the effects of herbs (Obosan-C or Obosan-O) as an additive in formulated diets on growth and body composition of juvenile abalone (Haliotis discus hannai), Three replicate groups of abalone initially average weighing 206 mg, 827 mg and 432 mg were fed the each one of experimental diet containing $0\%\;or\;1\%$ Obosan for 10 (experiment 1), 7 (experiment 2) and 19 weeks (experiment 3), respectively. In addition, an imported commercial diet for abalone was also employed to compare growth performance of juvenile abalone. Weight gain of abalone fed the diet containing Obosan-O was significantly (P<0.05) higher than that of abalone fed the control diet in the experiment 3. Moisture, crude protein and ash contents of soft whole body were not influenced by dietary herbs (P>0.05), whereas crude lipid content of abalone fed the diets containing Obosan-C was significantly (P<0.05) lower than that of abalone fed the control diet in the experiment 2. The results indicate that Obosan-O as an additive in formulated diet can improve body growth of juvenile abalone.