• Journal of Life Science
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• v.16 no.5
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• pp.711-715
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• 2006

The objective of this study was focused on the enhancement of pollen germination frequency in peach (Prunus $persica\;_{SIEB}$) under low temperature conditions. The effect of factors such as amino acid, polyamine, and flavonoid on the pollen germination was investigated, and the results are summarized as follows. When amino acid, polyamine or flavonoid was added to the germination medium at $10^{\circ}C$, pollen germination frequency was strongly promoted. Optimum concentration of each supplement for pollen germination enhancement was $100\;mg{\cdot}L^{-1}\;H_3BO_3$, 10 mM asparagine, 10 mM glutamine, 100 mM spermine, $1000\;{\mu}M$ putrescine, and $1.0\;{\mu}M$ kaemferol. The best combination of factors in pollen germination was $100\;mg{\cdot}L^{-1}\;H_3BO_3+10\;mM$ asparagine, followed by $100\;mg{\cdot}L^{-1}\;H_3BO_310mM$ glutamine, $100\;mg{\cdot}L^{-1}\;H_3BO_3+200mM$ spermine, and 10 mM asparagine. These combinations promoted pollen germination by 18% in 'Nagasawa-Hakuho', and 19% in 'Shuho' compared to their germination percentage on the basal medium.