• The Korean Journal of Mycology
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• v.21 no.4
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• pp.285-292
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• 1993

For the enzymatic conversion of 5'-XMP to 5'-GMP, partially purified XMP aminase from Escherichia coli was coupled with the yeast, Saccharomycrs cerevisiae, capable of ATP regeneration through glycolytic pathway. In order to elevate the level of XMP aminase in E. coli, $guaB^{-}(IMP\;dehydrogenase-less)$ mutant were introduced, and the yeast used as ATP supplier was treated by some method to increase its membrane permeability. The optimum conditions for efficient conversion reaction by energy-coupled system were investigated. As the results, a CH 41, $guaB^-$ mutant of E. coli K-12, showed 2.75 fold increase in the level of XMP aminase, compared with its parent cell. And the lyophylized yeast was the most effective at the ATP supplier. The optimum temperature and pH of conversion reaction were $40{\circ]C$ and pH 7.4, and the highest conversion ratio was shown under the reaction condition of 100 mM glucose, 100 mM inorganic phosphate and 6 mM AMP. When 36 units/ml XMP aminase used under the above conditions, the amount of 60 mg/ml yeast was sufficient to be used. Under the optimum condition, 71% of 1.8 mM(65.6 mg/100 ml) 5'-XMP was converted to 5'-GMP within 8 hr.

• Proceedings of the KSR Conference
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• 2011.05a
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• pp.910-917
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• 2011

CBTC(Communication Based Train Control) car-borne equipment, a part of the communication based train control system, mainly consists of automatic train protection(ATP) functions, automatic train operation(ATO) functions as well as the interface functions with other equipment including CBTC wayside equipment and train control management system etc. The CBTC car-borne software implementing ATP/ATO functions is a real-time embedded software requiring a high level of safety and reliability. To satisfy the requirements of the CBTC car-borne software, the model-based design techniques are applied with SCADE(Safety-Critical Application Development Environment) to the development of the CBTC car-borne software. In this paper, we illustrate the process modeling the car-borne ATP/ATO functions satisfying system requirement specification with system requirement management, modeling and document generation tools etc. supported by SCADE. In addition, the developed models corresponding to the ATP/ATO functions are applied to the train with CBTC car-borne equipment through its corresponding EN-50128 standards-compliant C code generated by the code generator. It is shown from the test result that the ATP/ATO models developed by SCADE work well while the trains are running in driverless operation mode.

• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.157-162
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• 1991

Conditions for glutathione production in E. coli cells which possess pGH501 (2 gshI+gshII) were studied. In terms of ATP supply for the glutathione synthesis, two different systems have been constructed and compared. When the acetate kinase reaction of E. coli was used for ATP generation, 20 mM of L-cysteine was completely converted to glutathione by toluene-treated E. coli cells (100 mg/ml) harboring pGH501 within 2 h at $37^{\circ}C$. However, considering the economical aspects, the glycolytic pathway of yeast was chosen as a better system for ATP generation. The optimal concentrations of reactants for glutathione production were determined to be as follows; 80 mM L-glutamate, 20 mM L-cysteine, 20 mM glycine, 20 mM $MgCl_2$, 50 mM potassium phosphate buffer (pH 7.5), 400 mM glucose, polyoxyethylene stearylamine ($5\;\mul/ml$), toluene-treated E. coli HB101/pGH501 (100 mg/ml), and dried yeast cells (400 mg/ml). The conversion ratio of L-cysteine to glutathione was 80% (about 5 mg/ml) under optimal condition within 6 h at $37^{\circ}C$.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.1092-1099
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• 2011

This paper is concerned with the ATO speed profile generation method in the moving block system. The ATO speed profile is calculated using the acceleration limit and the jerk limit to improve ride comfort. In addition, the speed limit and stop distance provided from ATP profile should be considered to ensure safety. In the moving block system, the speed limit and the stop position are frequently changed in real-time. Therefore, the ATO speed profile should be regenerated immediately according to change of the speed limit and the stop position, within the acceleration limit and the jerk limit. In this paper, the ATO speed profile generation method is proposed, which considers not only frequent changes of the speed limit and the stop position but also acceleration limit and jerk limit. Futhermore the simulation result is presented to verify usefulness of this method.

• Proceedings of the KSR Conference
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• 2009.05b
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• pp.631-637
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• 2009

The railway signaling systems operated in Korea are ATS system, ATP system for the low speed line and ATC system for the high speed line. The integrated onboard signaling system for the next-generation high-speed rail is progressed as the need for reducing a driver's load and controlling a train properly with the automatic translation of the onboard signaling system according to a trackside signaling system. In this paper the configuration of the integrated onboard signaling system and the interfaces of the subsystems is presented.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.54 no.6
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• pp.303-308
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• 2005

As the cost of photovoltaic(PV) generation systems continues to decrease, utility interactive systems are becoming more economically viable. Furthermore, increases in consumer awareness correspond to a willingness to pay a premium price for clean electrical energy generated using renewable energy resources. Especially, PV generation systems, in particular, is undergoing a rapid expansion-showing an industrial growth of approximately 40$\%$ per year in the worldwide, as PV cell and systems technology improve new markets become increasingly accessible. This has resulted in an increased demand for the simulation scheme and operational technologies of utility interactive PV devices and systems. The simulation schemes that can be applied to the utility interactive PV generation systems readily and cheaply under various conditions considering the sort of solar cell, the capacity of systems and the converter system as well are strongly expected and emphasized among researchers. So far, authors have been introducing the simulation method of PV generation systems with several papers. In this paper, authors introduce the simulation way of PV generation systems using EMTP type simulators; EMTP/ATP, EMTDC/PSCAD, RTDS, with each examples. And, by connecting the voltage amplifier to the RTDS a novel simulation method which is extremely close to the real condition of PV generation system is also introduced.

• Medical Lasers
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• v.10 no.4
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• pp.195-200
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• 2021

Evidence shows that nerve injury triggers mitochondrial dysfunction during axonal degeneration. Mitochondria play a pivotal role in axonal regeneration. Therefore, normalizing mitochondrial energy metabolism may represent an elective therapeutic strategy contributing to nerve recovery after damage. Photobiomodulation (PBM) induces a photobiological effect by stimulating mitochondrial activity. An increasing body of evidence demonstrates that PBM improves ATP generation and modulates many of the secondary mediators [reactive oxygen species (ROS), nitric oxide (NO), cyclic adenosine monophosphate (cAMP), and calcium ions (Ca²⁺)], which in turn activate multiple pathways involved in axonal regeneration.

• Proceedings of the KSR Conference
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• 2011.05a
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• pp.363-367
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• 2011

In this paper a simulator for Integrated Onboard Signalling System(IOSS) will be presented and illustrated. IOSS which is integrated with there signalling systems such as ERTMS/ETCS Level 1 ATP(Automatic Train Protection), ATC(Automatic Train Control) and ATS(Automatic Train Stop) is a signalling system for HEMU-400X(Highspeed Electric Multiple Unit - 400km/h eXperiment). HEMU-400X is under development as the next generation high-speed train in Korea. Before conducting a trial run of HEMU-400X with IOSS, we must carry out functional test of IOSS. The simulator is suggested in this paper for testing and verification of IOSS. The simulator can help to test all function of IOSS although a real train and trackside equipments are not existed. Also the simulator can make a fault in trackside equipment intentionally. In that scenario, we can figure out how IOSS handle emergency situations.

• Tuberculosis and Respiratory Diseases
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• v.43 no.2
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• pp.123-127
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• 1996

Secretion of surfactant phospholipid can be stimulated by a variety of agonists acting via at least three different signal transduction mechanisms. These include the adenylate cyclase system with activation of cAMP-dependent protein kinase; activation of protein kinase C either directly or subsequent to activation of phosphoinositide-specific phospholipase C and generation of diacylglycerols and inositol trisphosphate; and a third mechanism that involves incresed $Ca^{2+}$ levels and a calmodulin-dependent step. ATP stimulates secretion via all three mechanisms. The protein kinase C pathway is also coupled to phopholipase D which, acting on relatively abundant cellular phospholipids, generates diacylglycerols that further activate protein kinase C. Sustained protein kinase C activation can maintain phosphatidylcholine secretion for a prolonged period of time. It is likely that interactions between the different signaling pathways have an important role in the overall physiological regulation of surfactant secretion.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.823-829
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• 2002

The thrombosis importantly came to the front as the risk factor of these circulation system's disease. SilsoSanGami(SSG) was used for investigating the inhibitory effect on platelet-activating factor-induced platelet aggregation about drugs that used to improvement various symptoms created by the thrombosis in oriental medicine. In this study, the water-extracted SSG was investigated for its possible antithrombotic action on platelets. The antithrombotic activity of water-extracted SSG was deduced from its ability to suppress platelet aggregation, ATP-exocytosis, and the generation of prostaglandin E₂ and thromboxane A₂ by human platelets, stimulated with arachidonic acid. Water-extracted SSG dose-dependently suppressed the aggregation of human platelets, the release of endogenous ATP, and the formation of PGE₂ and TXB₂, both the latter usually detected to estimate the activity of COX and TXS, respectively. Since the IC/sub 50/ values necessary to inhibit COX (115 ㎍/㎖ SSG) and TXS(74 ㎍/㎖ SSG) were in the same range, inhibition of COX is suggested to be the primary target of water-extracted SSG, thus suppressing the formation of PGE₂ which is metabolized by TXS to TXA₂. We considerated that SSG has practical applicational value of clinical trial in the thrombosis caused by platelet aggregation.