• Animal Bioscience
• /
• v.37 no.8
• /
• pp.1463-1473
• /
• 2024

Objective: Three experiments were conducted to evaluate the effects of Saccharomyces yeast postbiotics (SYP) in feeds for sows on the growth of offspring (Exp. 1), for nursery pigs on their growth (Exp. 2), and for nursery and finishing pigs on their growth (Exp. 3). Methods: Exp. 1 had 80 sows at breeding assigned to 4 groups with SYP at 0, 0.050, 0.175, and 0.500 g/kg. Offspring were fed a common diet for 126 d. Exp. 2 had 144 barrows at 8 kg body weight (BW) allotted to CON (no SYP); YPC (SYP at 0.175 g/kg; d 0 to 42); and YPD (SYP at 1.25, 0.75, and 0 g/kg; d 0 to 7, d 8 to 21, and d 22 to 42, respectively) with 8 pens/treatment (6 pigs/pen). Exp. 3 had 96 barrows at 8 kg BW allotted to CON (no SYP); YPN (SYP at 0.175 g/kg; d 0 to 42); YPF (SYP at 0.100 g/kg; d 43 to 119); and YPA (SYP at 0.175 and 0.100 g/kg; d 0 to 42 and d 43 to 119, respectively) with 8 pens/treatment (3 pigs/pen). Results: In Exp. 1, increasing SYP increased (p<0.05, quadratic) the sow body score (maximum at 0.30% SYP), reduced (p<0.05, quadratic) the days-wean-to-estrus (minimum at 0.27% SYP), and increased (p<0.05) offspring BW at weaning and their average daily gain (ADG) and feed efficiency (G:F) at d 126. In Exp. 2, ADG, average daily feed intake (ADFI), and G:F of YPC were the greatest (p<0.05). The ADG and ADFI of YPD were greater (p<0.05) than CON. Fecal score of YPC and YPD was smaller (p<0.05) than CON. In Exp. 3, YPA had the greatest (p<0.05) ADG and YPN and YPF had greater (p<0.05) ADG than CON. Conclusion: SYP enhanced sow performance, offspring growth, growth of nursery and growing pigs with the greater efficacy at 0.27 to 0.32 g/kg feed.

• Korean Circulation Journal
• /
• v.54 no.8
• /
• pp.499-512
• /
• 2024

Background and Objectives: Arterial dissection during endovascular therapy rarely occurs but can be lethal. A fabric-based covered graft stents yield poor clinical outcomes. A novel balloon-expandable stent with biodegradable film graft for overcoming these issues was evaluated in a rabbit iliac artery model. Method: Eighteen rabbits with iliac artery dissections were induced by balloon over-inflation on angiography (Ellis type 2 or 3) and treated using the test device (3.0×24 mm). Subsequently, survived twelve animals underwent histologic examinations and micro-computed tomography (CT) at 0, 2, 4, and 8 weeks and 3, 6, 9, and 12 months and angiography at one-year. Results: There were no adverse cardiovascular events during the one-year. Early-stage histologic examination revealed complete sealing of disrupted vessels by the device, exhibiting mural hematoma, peri-stent red thrombi, and dense infiltration of inflammatory cells. Mid- and long-term histologic examination showed patent stents with neointimal hyperplasia over the stents (% area stenosis: 11.8 at 2 weeks, 26.1 at 1 month, 29.7 at 3 months, 49.2 at 9 months, and 51.0 at 1 year), along with mild peri-strut inflammatory response (Grade: 1-2 at mid-term and 0-1 at long-term). The graft film became scarcely visible after six months. Both CT and angiography revealed no instances of thrombotic occlusion or in-stent restenosis (% diameter stenosis: 5.7 at 2 weeks, 12.3 at 1 month, 14.2 at 3 months, 25.1 at 9 months, and 26.6 at 1 year). Conclusions: The novel balloon-expandable stent with a biodegradable film graft demonstrates feasibility in managing severe artery dissection and preventing lethal vascular events in animal model.

• Korean Journal of Poultry Science
• /
• v.27 no.1
• /
• pp.51-61
• /
• 2000

This study was conducted to estimate the general production performance, heritabilities, and genetic phenotypic correlations on several economic traits by multiple trait animal model in Korean native chicken. The data were collected from the records of 5,192 pullets produced from 2,029 dams and 250 sires in Daejon Branch, National Livestock Research Institute from April 10. 1994 to February 10. 1997. Body weights of Red Brown (RB), Yellow Brown (YB), Grey Brown (GB) and Black (B) strains were 1,649.9, 1,439.6, 1,455.6 and 1,575.3g at age of 150 days and 1,923.5, 1,689.6, 1,812.4 and 1,924.0g at age of 270 days, respectively. The ranges of coefficient of variation for body weight were 11.52 to 14.02 at age of 150 days and 12.82 to 14.59% at age of 270 days, respectively. The first eggs of RB, YB, GB and B strains were produced at 147.4, 146.5, 151.1 and 152.1 days, respectively and the coefficients of variation were 7.80 to 8.41%. Egg weight at 270 days were 49.7, 47.6, 48.8 and 48.7 g for RB, YB, GB nd B strains, and coefficients of variation were 6.99 to 7.94% . The number of eggs at age of 270days were 75.8, 78.0, 76.7 and 68.8 for RB, YB, GB and B strains, respectively, and coefficient of variations were 23.87 to 29.89%. On heritability estimates in RB, YB, GB and strains body weight were 0.40, 0.10, 0.09 and 0.57 for RB, YB, GB and B strains at age of 150 days, and 0.23, 0.09, 0.38 and 0.24 at age of 270 days were 0.39, 0.47, 0.60 and 0.62, and egg weights at 270 days and age at 1st egg were 0.36, 0.12, 0.38 and 0.26, number of egg production at 270 days were 0.44, 0.36, 0.58 and 0.49, respectively. The genetic correlation coefficients of body weight with the age of first egg, egg weight and number of egg production at 270days were -0.07∼0.67, -0.24∼0.70 and 0.12∼0.41, respectively ; age of first egg with number of egg production at 270days and egg weight were -0.75∼0.91 and 0.34∼0.97 ; and egg weight with number of egg production at 270days were 0.18∼0.68. The phenotypic correlation coefficients of body weight with at age of first egg, egg weight and number of egg production at 270days were -0.01∼0.74, -0.04∼0.72 and 0.25∼0.57 ; age of first egg with number of egg production at 270days and egg weight were 0.26∼0.52 and 0.52∼0.92, respectively ; and egg weight with number of egg production at 270days were 0.34∼0.91.

• Child Health Nursing Research
• /
• v.6 no.2
• /
• pp.132-143
• /
• 2000

The purpose of this correlational study was to find the correlation between the infant temperament, mother-infant interaction, and child-rearing environment. The subjects of this study were 37 dyads of healthy mothers and healthy infants. Data were collected from 15th of March to 3rd of September, 1999. Convenient sampling was done at Obstetric wards of one University hospital, and demographic data were collected before discharge. At one month and three month postpartum, we visited subject's home, and collected the data on the infant temperament, and also video taped the mother-infant interaction during feeding. In addition, child-rearing environment was checked by researcher according to HOME(Home Observation for Measurement of the Environment) at three months postpartum. Mother-infant interactions were rated according to the NCAST Feeding Scale later. Data were analyzed by window SPSS program, and correlations between the infant temperament, mother-infant interaction, and child-rearing environment were analyzed by Pearson's correlational coefficient. The results were as follows: 1. Infant temperament. 1) Among the subscales of infant temperament, mothers perceived cuddliness and amenability most positively at one month, and responsivity and amenability most positively at three months. 2) In subscale analysis of stability, amenability, responsivity, and persistence were stable with the time. 3) Significant relationships were found between the malleability and amenability, between the malleability and responsivity at one month, and also between the malleability and amenability, and between the malleability and cuddliness at three months. 2. Correlations of infant temperament and mother-infant interaction. 1) There was no significant relationship between the infant temperament and mother-infant interaction at one month. 2) There was a significant relationship between the infant temperament and mother-infant interaction at three months(r=.335, p<.05). In subscale analysis, there were significnt relationships between the total score of infant temperament and maternal sensitivity to infant's cues(r=.372, p<.05), and between the total score of infant temperament and maternal response to infant's distress (r=.331, p<.05). 3. Correlations of infant temperament and child-rearing environment. 1) There was no significant relationship between the total score of infant temperament at one month and total score of HOME at three months. In subscal analysis, the total score of infant temperament at one showed significant relationships with the organization of environment(r=.413, p<.05), and the emotional, verbal response at three months(r=.337, p<.05). 2) There was a significant relationship between the total score of infant temperament at three months and the total score of HOME at three months (r=.599, p<.01). In subscal analysis, the total score of infant temperament at three months showed significant relationships with the organization of environment(r=.410, p<.05), maternal involvement(r=.482, p,.01), and the emotional, verbal response(r=.695, p<.01) at three months. 4. Correlations of mother-infant interaction and child-rearing environment. There was a significant relationship between the maternal score of mother-infant interaction at one month and three months and the total score of HOME at three months (r=.474, p<.01; r=.452, p<.01). In conclusion, it was proved that infant temperament had significant relationships with mother-infant interaction and child-rearing environment, especially when the infants were getting older. This showed the possibility for changeability of infant teperament by the maternal factors.

• Toxicological Research
• /
• v.15 no.1
• /
• pp.79-87
• /
• 1999

To know the stress response and antioxidative effect of sulfur containing compounds, we observed the expression of the stress protein (heat shock protein; inducible protein) from mouse tissues and evaluated the protective effects to hydroxyl radical in mouse brain cell culture. Cysteine, methionine or sodium sulfide was fed by oral administration of 1 ml/per 6hr/three times with 1 mM, 2mM or 3mM to mouse, respectively. After that, the stress proteins were extracted from mouse tissues and analyzed the features of expression. The stress proteins by sulfur containing compounds were showed different aspects in the kinds and concentrations of their compounds, and in the tissues of mouse. In the liver, the stress proteins were appeared at different time on the concentration of sulfur containing compounds and had less than 20 KDa as small molecules. In general, the molecular weights of stress protein in liver, the stress proteins were appeared at different time on the concentration of sulfur containing compounds and had less than 20 KDa as small molecules. In general, the molecular weights of stress protein in the spleen were evaluated from 32KDa to 50KDA, and the induced times were relatively late at high concentration of cysteine, early at low concentration of methionine or sodium sulfide. The stress proteins in mouse muscle were detected mostly between 24hr after treatment of sulfur containing compounds. Their molecular weights were 15~24KDa. In the antioxidative effects of sulfur containing compounds to hydroxyl radical, cell viabilities were measured by 63.2% at 10 $\mu\textrm{M}$, 65.5% at 50 $\mu\textrm{M}$, 68.6% at 100 $\mu\textrm{M}$, 78.3% at 150 $\mu\textrm{M}$, or 83.0% at 200 $\mu\textrm{M}$ of cysteine, respectively. At addition of methionine, the cell viabilities were assessed as 58.1% at 10 $\mu\textrm{M}$, 62.8% at 50 $\mu\textrm{M}$, 75.7% at 100 $\mu\textrm{M}$, 78.6% at 150 $\mu\textrm{M}$, and 79.2% at 200 $\mu\textrm{M}$ after 4hrs exposure with 20mU/ml glucose oxidase (GO) system, while the numbers of live cells to hydroxyl radicals in treatment of sodium sulfide were showed 48.6% at 10 $\mu\textrm{M}$, 54.8% at 100 $\mu\textrm{M}$, 51.8% at 150 $\mu\textrm{M}$, and 51.6% at 200 $\mu\textrm{M}$ in the neuronal cells. In the inhibitory effects on the proliferation of tumor cells, percentages of dead cells of the CT-26 or HeLa cell were generally less than 30% even 48hr after addition of sulfur containing compounds. Conclusively, the results of these experiments indicate that stress protein by sulfur containing compounds can be used as physiological indicator for animal nutrition and for environment, and also that cysteine and methionine can play critical roles as an antioxidant.

• Korean Journal of Veterinary Research
• /
• v.15 no.2
• /
• pp.187-198
• /
• 1975

Although considerable research has been done on the blood chemistry of domestic animals, little work has been made of the changes associated with age. Moreover, the records about physiology of the goat were not much available in Korea, and a comprehensive survey of the blood values of the Korean native goat has not been made. The object of the present investigation was to make good this deficiency and to suggest standards for the blood chemical values of Korean native goats from birth to maturity. The goats were kept under average farming conditions in Korea. Blood samples were collected from the jugular vein at birth, at one and four days, at one, two, three and four weeks, and at two, three, six, nine and twelve months of age. The results obtained in this work were summarized as follows: 1. The highest concentration of serum glucose was observed at birth and it decreased gradually until three months of age, showing a steady state thereafter. 2. The concentration of total serum protein was a little higher at one day of age than at birth and showed a steady state until seven days of age; then it decreased slightly at two weeks of age and recovered at three weeks of age and showed a steady state until twelve months of age. The concentration of serum albumin was increased a little at one day of age and revealed a steady state thereafter. The concentration of serum globulin showed three phases - a fall during the first two weeks to reach minimum, a rise to the fourth week, and a fall at two months of age, showing a steady state thereafter. The lowest value of albumin fraction was observed at birth, then the value increased gradually until three months of age and revealed a stealer state thereafter. The highest value of globulin fraction was observed at birth, then the gradual decrease of the value was seen until three months of age; thereafter the value showed a steady state. 3. Albumin/globulin ratio was the minimum at birth, then it increased to reach a maximum at two weeks of age and decreased a little thereafter. 4. The concentration of total serum cholesterol showed a gradual increase during the first three months and fell to reach adult revel at six months of age. 5. Urea nitrogen in serum decreased during the first week to reach a minimum, then it increased at three months of age, and showed a fell to mature level at six mouths of age. 6. The concentration of serum creatinine was not affected by age. 7. The concentration of total serum calcium was a little higher during the first two weeks than the other period during the first year of life. 8. The concentration of serum inorganic phosphorus showed a gradual rise to reach a maximum and a gradual fall to reach adult level at nine months of age. 9. No significant sex differences of serum chemical values were recognized.

• Journal of Biosystems Engineering
• /
• v.26 no.4
• /
• pp.315-322
• /
• 2001

Agricultural products can be damaged due to the vibration of transporting trailer on the off-road. So, this study was conducted to identify the vibration characteristics of the agricultural products transporting trailer by measuring the vertical acceleration according to positions on the trailer loaded with agricultural products. The results of this study can be summarized as follows: 1. At non-operating state of engine, the larger vertical acceleration was occurred at rear side compared with front side in the case of 4.5Hz of vibration frequency. But, in the case of 53.5Hz of frequency, the maximum vertical acceleration at front side of trailer was higher than value at rear side. So, the maximum acceleration at front side of the trailer was increased with the increase in frequency. 2. At operating state of engine, the maximum vertical acceleration at front side of the trailer was increased with the increase in frequency. 2 At operating state of engine, the maximum vertical acceleration delivered through the hitch from the engine was occurred at front side of the trailer as $3.0\times10^{-3}m/s^2$, in the case of 8.75Hz of frequency. But, in the case of 102.5Hz of high frequency, the maximum vertical acceleration was occurred at rear side of the trailer. 3. When the power tiller loaded with pear of 325kg was travelling on the artificial uneven road of 3cm height, the maximum acceleration was occurred at rear side of the trailer as $4.7\times10^{-3}m/s^2$at 3.75Hz of frequency. But, that was occurred at diagonal of the trailer 43.5Hz and 91.25Hz, which meant that there was rolling and pitching on the trailer. 4. At operating state of engine, the mean acceleration of the trailer delivered through the hitch according to the increase in frequencies was showed the maximum value at range of 40-90Hz. At rear side of traiㅣer, the maximum value was occurred at about 40Hz, and that was reduced according to the increase in frequencies and diminished at about 100Hz. 5. When the power tiller loaded with pear of 32.5kg was travelling on the artificial uneven road of 3cm height, the mean acceleration by the increase in frequencies was showed lower level at rear side than front side of the trailer. This was opposite configuration to the Hinsch’s results tested with air-conditioned truck. This means that the shorter length of the trailer, the more effect of engine vibration is transferred to the front side of trailer.

• Journal of agricultural medicine and community health
• /
• v.23 no.2
• /
• pp.205-213
• /
• 1998

In order to find out the status of routine-immunization in a rural area, this study was performed, through analyzing the data which was obtained from the immunization register of infants who was born at Su-Dong myun in 1996 and 1997, managed by Su-dong Myun health subcenter. The results are as follows. 1. B.C.G immunization rate was the highest such as 52.2% within 1 month and next order such 34.8% at 2 month in 1996. In 1997, the highest such as 73.8%, almost all, within 1 month. 2. D.P.T immunization rate in 1996 showed, almost all, the highest at 3 month(79.4%) for 1st dose and at 5 month(78.4%) for 2nd dose. However, the rate for 3rd dose showed the highest at 7 month(51.4%), and next order at 8 month(35.1%) and at 6 month(13.5%). D.P.T immunization rate in 1997, similarly showed the highest at 3 month(81.8%) for 1st dose, at 5 month(71.2%) for 2nd dose and at 7 month(71.4%) for 3rd dose. 3. Hepatitis B immunization rate showed the highest at birth at once or within one week(87.0%) for 1st dose in 1996 and (94.7%) in 1997. The rate for 2nd dose showed the highest at 2 month(51.7%) in 1996 and (50.0%) in 1997, and next order at 1 month(44.8%) in 1996 and (34.4%) in 1997. The rate for 3rd dose showed the highest at 3 month(54.8%) in 1996 and 5 month(54.8%) in 1997, and next order at 5 month(25.8%) in 1996 and at 3 month(26.0%) in 1997. 4. Measles immunization rate was 76.1% in 1996. The rate(76.1%) by the kind of vaccine was the highest with measles-MMR(34.8%), and with MMR(32.6%) and next order with measles(8.7%). The rate by measles immunization time(month) was the highest such as 35.0% at 9 month and 10 month respectively and the rate by MMR was the highest at 16 month(35.5%), and 15 month(22.5%), 13 month (12.9%) and 14 month(12.9%) in next order.

• Bulletin of the Korean Chemical Society
• /
• v.22 no.2
• /
• pp.164-170
• /
• 2001

The crystal structures of fully dehydrated Ni2+- and Tl+ -exchanged zeolite X (Ni17Tl58-X, and Ni12Tl68-X; X=Si100Al92O384) have been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd3 at $21(1)^{\circ}C$ (a=24.380(4) $\AA$, 24.660(4) $\AA$, respectively). Their structures have been refined to the final error indices R1=0.037 and R2=0.043 with 485 reflections, and R1=0.039 and R2=0.040 with 306 reflections, respectively, for which I >36(I). In Ni17Tl58-X, 17 Ni2+ ions per unit cell were found at only two sites: 15 at site I at the center of the hexagonal prism (Ni-O=2.203(9) $\AA)$ and the remaining 2 at site II near single six-oxygen rings in the supercage (Ni-O=2.16(3) $\AA).$ Fifty-eight Tl+ ions were found at five crystallographic sites: 28 at site II (Tl-O=2.626(8) $\AA)$, 2 at site I' in the sodalite cavity near the hexagonal prism (Tl-O=2.85(1) $\AA)$, another 2 at site II' in the sodalite cavity (Tl-O=2.77(1) $\AA).$ The remaining 26 were found at two nonequivalent Ⅲ' sites with occupancies of 23 and 3. In Ni12Tl68-X, 12 Ni2+ ions per unit cell were found at two sites: 10 at site I (Ni-O=2.37(2) $\AA)$ and the remaining 2 at site II (Ni-O=2.13(2) $\AA).$ Sixty-eight Tl+ ions were found at five crystallographic sites: 28 at site II (Tl-O=2.63(1) $\AA)$, 12 at site I' (Tl-O=2.62(1) $\AA)$, 2 at site II' (Tl-O=3.01(2) $\AA)$, and the remaining 26 at two III' sites with occupancies of 23 and 3. It appears that Ni 2+ ions prefer to occupy site I and II, in that order. The large Tl+ ions occupy the remaining sites, I', II, II' and two different III' sites. In both crystals, only the Ni2+ ions at site II were reduced and migrated to the external surface of zeolite X when these crystals were treated with hydrogen gas.

• Radiation Oncology Journal
• /
• v.17 no.4
• /
• pp.299-306
• /
• 1999

Purpose : The human genetic disorder ataxia-telangiectasia (AT) is a multisystem disease characterized by extreme radiosensitivity. The recent identification of the gene mutated in AT, ATM, and the demonstration that it encodes a homologous domain of phosphatidylinositol 3-kinase (PI3-K), the catalytic subunit of an enzyme involved in transmitting signals from the cell surface to the nucleus, provide support for a role of this gene in signal transduction. Although ionizing radiation was known to induce c-fos transcription, nothing is known about how ATM or PKCI mediated signal transduction pathway modulates the c-fos gene transcription and gene expression. Here we have studied the effect of PKCI on radiation sensitivity and c-fos transcription in normal and AT cells. Materials and Methods: Normal (LM217) and AT (AT5BIVA) cells were transfected with PKCI expression plasmid and the overexpression and integration of PKCI was evaluated by northern blotting and polymerase chain reaction, respectively. 5 Gy of radiation was exposed to LM and AT cells transfected with PKCI expression plasmid and cells were harvested 48 hours after radiation and investigated apoptosis with TUNEL method. The c-fos transcription activity was studied by performing CAT assay of reporter gene after transfection of c-fos CAT plasmid into AT and LM cells. Results: Our results demonstrate for the first time a role of PKCI on the radiation sensitivity and c-fos expression in LM and AT cells. PKCI increased radiation induced apoptosis in LM cells but reduced apoptosis in AT cells. The basal c-fos transcription activity is 70 times lower in AT cells than that in LM cells. The c-fos transcription activity was repressed by overexpression of PKCI in LM cells but not in AT cells. After induction of c-fos by Ras protein, overexpression of PKCI repressed c-fos transcription in LM cells but not in AT cells Conclusion: Overexpression of PKCI increased radiation sensitivity and repressed c-fos transcription in LM cells but not in AT cells. The results may be a. reason of increased radiation sensitivity of AT cells. PKCI may be involved in an ionizing radiation induced signal transduction pathway responsible for radiation sensitivity and c-fos transcription. The data also provided evidence for novel transcriptional difference between LM and AT cells.